LOCUS       sequence-356067-        8613 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encoding E. coli BetaGalactosidase with an N-terminal M9 
            nuclear localization sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8613)
  AUTHORS   Chowdhury R, Sau A, Musser SM
  TITLE     Super-resolved 3D tracking of cargo transport through nuclear pore 
            complexes.
  JOURNAL   Nat Cell Biol. 2022 Jan;24(1):112-122. doi: 
            10.1038/s41556-021-00815-6. Epub 2022 Jan 10.
  PUBMED    35013558
REFERENCE   2  (bases 1 to 8613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-021-00815-6"; journalName: "Nat Cell Biol"; date: 
            "2022-01"; volume: "24"; issue: "1"; pages: "112-122"
COMMENT     Sequence Label: pM9-BetaGal-8C
FEATURES             Location/Qualifiers
     source          1..8613
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      48..503
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(135..154)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     345..366
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(595..1410)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(595..1410)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1318..1337
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1402..1421
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1532..2120
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2021..2040
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2274..2291
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2306..2445
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2371..2390)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2531..2553
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2547..2738)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3547..4629)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4591..4610
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4630..4707)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     4913..4932
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     5020..5039
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5020..5038
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5039..5063
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5078..5100
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5111..5128
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5138..5155
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             5162..5206
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             5222..5236
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             5284..5295
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             8493..8510
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8563..8581)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      join(8577..8613,1..11)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag
       61 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      121 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
      181 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
      241 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
      301 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
      361 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
      421 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
      481 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
      541 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgaatta attcttagaa
      601 aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata
      661 tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat
      721 ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa
      781 tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc
      841 cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt
      901 acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg
      961 agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa
     1021 ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc
     1081 taatacctgg aatgctgttt tcccggggat cgcagtggtg agtaaccatg catcatcagg
     1141 agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct
     1201 gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc
     1261 tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc
     1321 gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctaga
     1381 gcaagacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc
     1441 agacagtttt attgttcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1501 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1561 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1621 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1681 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1741 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1801 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1861 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1921 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1981 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     2041 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     2101 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     2161 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     2221 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     2281 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
     2341 gtatttcaca ccgcaatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     2401 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
     2461 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     2521 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     2581 aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt
     2641 tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag
     2701 cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga
     2761 tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt
     2821 actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg
     2881 atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat
     2941 gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg
     3001 cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat
     3061 gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc
     3121 ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac
     3181 aggagcacga tcatgcgcac ccgtggggcc gccatgccgg cgataatggc ctgcttctcg
     3241 ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg
     3301 aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa
     3361 atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata
     3421 agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct
     3481 ctcaagggca tcggtcgaga tcccggtgcc taatgagtga gctaacttac attaattgcg
     3541 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     3601 ggccaacgcg cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac
     3661 cagtgagacg ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa
     3721 gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg
     3781 gatataacat gagctgtctt cggtatcgtc gtatcccact accgagatgt ccgcaccaac
     3841 gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac
     3901 cagcatcgca gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga
     3961 catggcactc cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata
     4021 tttatgccag ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag
     4081 cgcgatttgc tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc
     4141 atgggagaaa ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg
     4201 aacattagtg caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat
     4261 gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac
     4321 gccgcttcgt tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt
     4381 aatcgccgcg acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat
     4441 cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc
     4501 cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac
     4561 cacgcgggaa acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac
     4621 tggtttcaca ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg
     4681 aaaggttttg cgccattcga tggtgtccgg gatctcgacg ctctccctta tgcgactcct
     4741 gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg
     4801 gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca
     4861 cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt
     4921 cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc
     4981 cggcgtagag gatcgagatc gatctcgatc ccgcgaaatt aatacgactc actatagggg
     5041 aattgtgagc ggataacaat tcccctctag aaataatttt gtttaacttt aagaaggaga
     5101 tatacatatg caccatcatc atcatcattc ttctggtctg gtgccacgcg gttctggtat
     5161 gaaagaaacc gctgctgcta aattcgaacg ccagcacatg gacagcccag atctgggtac
     5221 cgacgacgac gacaaggcca tggctgatat cggatccgga ggtggtggaa gctacaatga
     5281 ttttgggaat tacaacaatc agtcttcaaa ttttggaccc atgaagggag gaaattttgg
     5341 aggcagaagc tctggcccct atggcggtgg aggccaatac tttgcaaaac cacgaaacca
     5401 aggtggctat ggcggttcca gcagcagcag tagctatggc agtggcagaa gatttggggg
     5461 cgggctggta ccgaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     5521 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt
     5581 tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg ctggaggccg atcttcctga
     5641 ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac ggttacgatg cgcccatcta
     5701 caccaacgtg acctatccca ttacggtcaa tccgccgttt gttcccacgg agaatccgac
     5761 gggttgttac tcgctcacat ttaatgttga tgaaagctgg ctacaggaag gccagacgcg
     5821 aattattttt gatggcgtta actcggcgtt tcatctgtgg tgcaacgggc gctgggtcgg
     5881 ttacggccag gacagtcgtt tgccgtctga atttgacctg agcgcatttt tacgcgccgg
     5941 agaaaaccgc ctcgcggtga tggtgctgcg ctggagtgac ggcagttatc tggaagatca
     6001 ggatatgtgg cggatgagcg gcattttccg tgacgtctcg ttgctgcata aaccgactac
     6061 acaaatcagc gatttccatg ttgccactcg ctttaatgat gatttcagcc gcgctgtact
     6121 ggaggctgaa gttcagatgt gcggcgagtt gcgtgactac ctacgggtaa cagtttcttt
     6181 atggcagggt gaaacgcagg tcgccagcgg caccgcgcct ttcggcggtg aaattatcga
     6241 tgagcgtggt ggttatgccg atcgcgtcac actacgtctg aacgtcgaaa acccgaaact
     6301 gtggagcgcc gaaatcccga atctctatcg tgcggtggtt gaactgcaca ccgccgacgg
     6361 cacgctgatt gaagcagaag cctgcgatgt cggtttccgc gaggtgcgga ttgaaaatgg
     6421 tctgctgctg ctgaacggca agccgttgct gattcgaggc gttaaccgtc acgagcatca
     6481 tcctctgcat ggtcaggtca tggatgagca gacgatggtg caggatatcc tgctgatgaa
     6541 gcagaacaac tttaacgccg tgcgctgttc gcattatccg aaccatccgc tgtggtacac
     6601 gctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc aatattgaaa cccacggcat
     6661 ggtgccaatg aatcgtctga ccgatgatcc gcgctggcta ccggcgatga gcgaacgcgt
     6721 aacgcgaatg gtgcagcgcg atcgtaatca cccgagtgtg atcatctggt cgctggggaa
     6781 tgaatcaggc cacggcgcta atcacgacgc gctgtatcgc tggatcaaat ctgtcgatcc
     6841 ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc acggccaccg atattatttg
     6901 cccgatgtac gcgcgcgtgg atgaagacca gcccttcccg gctgtgccga aatggtccat
     6961 caaaaaatgg ctttcgctac ctggagagac gcgcccgctg atcctttgcc aatacgccca
     7021 cgcgatgggt aacagtcttg gcggtttcgc taaatactgg caggcgtttc gtcagtatcc
     7081 ccgtttacag ggcggcttcg tctgggactg ggtggatcag tcgctgatta aatatgatga
     7141 aaacggcaac ccgtggtcgg cttacggcgg tgattttggc gatacgccga acgatcgcca
     7201 gttctgtatg aacggtctgg tctttgccga ccgcacgccg catccagcgc tgacggaagc
     7261 aaaacaccag cagcagtttt tccagttccg tttatccggg caaaccatcg aagtgaccag
     7321 cgaatacctg ttccgtcata gcgataacga gctcctgcac tggatggtgg cgctggatgg
     7381 taagccgctg gcaagcggtg aagtgcctct ggatgtcgct ccacaaggta aacagttgat
     7441 tgaactgcct gaactaccgc agccggagag cgccgggcaa ctctggctca cagtacgcgt
     7501 agtgcaaccg aacgcgaccg catggtcaga agccgggcac atcagcgcct ggcagcagtg
     7561 gcgtctggcg gaaaacctca gtgtgacgct ccccgccgcg tcccacgcca tcccgcatct
     7621 gaccaccagc gaaatggatt tttgcatcga gctgggtaat aagcgttggc aatttaaccg
     7681 ccagtcaggc tttctttcac agatgtggat tggcgataaa aaacaactgc tgacgccgct
     7741 gcgcgatcag ttcacccgtg caccgctgga taacgacatt ggcgtaagtg aagcgacccg
     7801 cattgaccct aacgcctggg tcgaacgctg gaaggcggcg ggccattacc aggccgaagc
     7861 agcgttgttg cagtgcacgg cagatacact tgctgatgcg gtgctgatta cgaccgctca
     7921 cgcgtggcag catcagggga aaaccttatt tatcagccgg aaaacctacc ggattgatgg
     7981 tagtggtcaa atggcgatta ccgttgatgt tgaagtggcg agcgatacac cgcatccggc
     8041 gcggattggc ctgaactgcc agctggcgca ggtagcagag cgggtaaact ggctcggatt
     8101 agggccgcaa gaaaactatc ccgaccgcct tactgccgcc tgttttgacc gctgggatct
     8161 gccattgtca gacatgtata ccccgtacgt cttcccgagc gaaaacggtc tgcgctgcgg
     8221 gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc gacttccagt tcaacatcag
     8281 ccgctacagt caacagcaac tgatggaaac cagccatcgc catctgctgc acgcggaaga
     8341 aggcacatgg ctgaatatcg acggtttcca tatggggatt ggtggcgacg actccctgag
     8401 cccgtcagta tcggcggaat tccagctgag cgccggtcgc taccattacc agttggtctg
     8461 gtctcaaaaa taagcttgcg gccgcactcg agcaccacca ccaccaccac tgagatccgg
     8521 ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag
     8581 cataacccct tggggcctct aaacgggtct tga
//
