LOCUS       sequence-356318-        2034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SHIP1-delta-C2-Enzyme domain flanked by RBP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Sudan R, Fernandes S, Srivastava N, Pedicone C, Meyer ST, Chisholm 
            JD, Engelman RW, Kerr WG
  TITLE     LRBA Deficiency Can Lead to Lethal Colitis That Is Diminished by 
            SHIP1 Agonism.
  JOURNAL   Front Immunol. 2022 May 4;13:830961. doi: 10.3389/fimmu.2022.830961.
            eCollection 2022.
  PUBMED    35603158
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Immunol."; date: "2022-05-4"; volume: "13:830961. doi"; pages: " 
            10.3389/fimmu.2022.830961. eCollection 2022"
COMMENT     Sequence Label: pS97splitRBP_S1dC2
FEATURES             Location/Qualifiers
     source          1..2034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             631..654
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     RBS             1084..1092
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             1690..1713
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 atgggtagct cacaccatca ccatcaccat tcgagctcgg gcgacgtagt gtcgcatatc
       61 gcaagcgata acgtcaaggg aggtgagatg gctgccgagt tcattgcgaa agccttaaag
      121 ggcaaaggga acgtcgtgga attagagggc atcccaggag cgtcggcagc tcgtgaccgc
      181 ggaaaagggt ttgacgaggc tattgctaag tacccagaca tcaagatcgt ggccaaacaa
      241 gccgccgact ttgatcgcag taagggtttg agcgttatgg aaaacatctt acaggcgcaa
      301 ccgaaaattg atgcagtgtt cgcgcaaaat gatgagatgg ctttgggtgc aattaaagca
      361 attgaggctg cgaaccgcca aggtattatc gtggtcggct tcgacggtac agaggacgcg
      421 ttgaaggcga ttaaagaagg gaagatggcg gcgaccattg cacaacagcc ggccttgatg
      481 ggctcgcttg gggtcgagat ggctgataaa tatcttaagg gcgaaaaaat ccctaatttt
      541 atccccgctg aattgaaatt gatcactaaa gaaaatgtgc aagggggttc tgcctctgga
      601 gggacaagtg gtggatcgag tgcggccggt ttggaggttt tattccaggg tccagcggcc
      661 gcgaacaagc actcagagca gccggagccc gacatgatca ccatcttcat cggcacctgg
      721 aacatgggta acgccccccc tcccaagaag atcacgtcct ggtttctctc caaggggcag
      781 ggaaagacgc gggacgactc tgcggactac atcccccatg acatttacgt gatcggcacc
      841 caagaggacc ccctgagtga gaaggagtgg ctggagatcc tcaaacactc cctgcaagaa
      901 atcaccagtg tgacttttaa aacagtcgcc atccacacgc tctggaacat ccgcatcgtg
      961 gtgctggcca agcctgagca cgagaaccgg atcagccaca tctgtactga caacgtgaag
     1021 acaggcattg caaacacact ggggaacaag ggagccgtgg gggtgtcgtt catgttcaat
     1081 ggaacctcct tagggttcgt caacagccac ttgacttcag gaagtgaaaa gaaactcagg
     1141 cgaaaccaaa actatatgaa cattctccgg ttcctggccc tgggcgacaa gaagctgagt
     1201 ccctttaaca tcactcaccg cttcacgcac ctcttctggt ttggggatct taactaccgt
     1261 gtggatctgc ctacctggga ggcagaaacc atcatccaga aaatcaagca gcagcagtac
     1321 gcagacctcc tgtcccacga ccagctgctc acagagagga gggagcagaa ggtcttccta
     1381 cacttcgagg aggaagaaat cacgtttgcc ccaacctacc gttttgagag actgactcgg
     1441 gacaaatacg cctacaccaa gcagaaagcg acagggatga agtacaactt gccttcctgg
     1501 tgtgaccgag tcctctggaa gtcttatccc ctggtgcacg tggtgtgtca gtcttatggc
     1561 agtaccagcg acatcatgac gagtgaccac agccctgtct ttgccacatt tgaggcagga
     1621 gtcacttccc agtttgtctc caagaacggt cccgggactg ttgacagcca aggacaggga
     1681 tccggtggct tagaagtatt attccaaggt ccaggttcgt caggagggac ggcatctgga
     1741 gggaaagaag gcaaaaccat cggtctggtc atcagtacct tgaataaccc cttctttgta
     1801 accttgaaaa atggcgctga ggagaaagcg aaggaactgg gatacaagat catcgtagag
     1861 gattcccaga atgactcctc gaaggagtta tccaacgttg aggatttaat tcaacagaag
     1921 gtcgacgtac tgctgattaa ccctgtagat tcggatgctg ttgtaaccgc cattaaggaa
     1981 gcaaatagca agaacattcc agttattact attgatcgct cggccaacgg gtaa
//
