LOCUS       sequence-356436-       10456 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of the cAMP sensor cAMPFIRE-H under a CAG 
            promotor w/ WPRE translation enhancer..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10456)
  AUTHORS   Massengill CI, Bayless-Edwards L, Ceballos CC, Cebul ER, Cahill J, 
            Bharadwaj A, Wilson E, Qin M, Whorton MR, Baconguis I, Ye B, Mao T, 
            Zhong H
  TITLE     Sensitive genetically encoded sensors for population and subcellular
            imaging of cAMP in vivo.
  JOURNAL   Nat Methods. 2022 Nov;19(11):1461-1471. doi: 
            10.1038/s41592-022-01646-5. Epub 2022 Oct 27.
  PUBMED    36303019
REFERENCE   2  (bases 1 to 10456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-022-01646-5"; journalName: "Nat Methods"; date: 
            "2022-11"; volume: "19"; issue: "11"; pages: "1461-1471"
COMMENT     Sequence Label: pCAGGS_cAMPFIRE-H
FEATURES             Location/Qualifiers
     source          1..10456
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          661..1677
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1601..1623
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1685..1704
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1754..1763
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1758..1767
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1772..1774,1775..1777,1778..2290)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIE"
     CDS             4676..5410
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     CDS             5468..6202
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     intron          6351..6483
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     6466..6485
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    6492..7080
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6545..6565)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6963..6974)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7107..7124)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7113..7224
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7320..7340)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7320..7338)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7359..7375)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7477..7496)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7542..7597
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7596..7615)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7958..7974)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7982..7998
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8006..8012,8013..8030,8031..8036))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8051..8072
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8131..8326
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8177..8312
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8332..8466
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8369..8388)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8423..8442
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8534..8551)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8705..9293)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8785..8804)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9464..10255,10256..10324))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10087..10106
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10325..10429)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggsgcgcg ccrggsgggg gggggggggg sggggggsgg
      541 ggsggggsgr ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
      601 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
      661 ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg
      721 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct
      781 ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa
      841 agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc
      901 gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc
      961 tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg
     1021 ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
     1081 tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac
     1141 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt
     1201 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
     1261 gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc
     1321 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
     1381 cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc
     1441 ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
     1501 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg
     1561 cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
     1621 accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
     1681 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt cggtaccgcg
     1741 ggcccagctt gccgccacca tggcggccgc aatggtgagc aagggcgagg agctgttcac
     1801 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     1861 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     1921 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgtcct ggggcgtgca
     1981 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     2041 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     2101 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     2161 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactact ttagcgacaa
     2221 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca
     2281 caacatcgag gacggcggcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     2341 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa
     2401 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgcccgata
     2461 tcagcccgtg ggaactcatg agatggagga ggagttggcc gaagctgtgg ccctgctctc
     2521 ccagcggggg cctgacgccc tgctcactgt ggcacttcga aagcccccag gtcagcgcac
     2581 ggatgaagag ctggacctca tctttgagga gctgctgcac atcaaggctg tggcccacct
     2641 ctccaactcg gtgaagcgag aattagcggc tgttctgctc tttgaaccac acagcaaggc
     2701 agggaccgtg ttgttcagcc agggggacaa gggcacttcg tggtacatta tctggaaggg
     2761 atctgtcaac gtggtgaccc atggcaaggg gctggtcacc accctgcatg agggagatga
     2821 ttttggagag ctggctctgg tgaatgatgc accccgggca gccaccatca tcctgcgaga
     2881 agacaactgt catttcctgc gtgtggacaa gcaggacttc aaccgtatca tcaaggatgt
     2941 ggaggcaaag accatgcggc tggaagaaca tggcaaagtg gtgctggtgc tgacaagagc
     3001 ctctcagggc gccggccctt cccgaccccc aaccccaggc aggaaccggt atacagtgat
     3061 gtctggcacc ccagagaaga tcctagagct tctgttggag gccatgggac cagattccag
     3121 tgctcatgac ccaacagaga cattcctcag cgacttcctc ctgacccaca gggtcttcat
     3181 gcccagcgcc caactctgcg ctgcccttct gcaccacttc catgtggagc ctgcgggtgg
     3241 cagcgagcag gagcgcagca cctacgtctg caacaagagg cagcagatct tgcggctggt
     3301 cagccagtgg gtggccctgt atggctccat gctccacact gaccctgtgg ccaccagctt
     3361 cctccagaaa ctctcagacc tggtgggcag ggacacccga ctcagcaacc tgctgaggga
     3421 gcagtggcca gagaggcggc gatgccacag gttggagaat ggctgtggga atgcatctcc
     3481 tcagatgaag gcccggaact tgcctgtttg gctccccaac caggacgagc cccttcctgg
     3541 cagcagctgt gccatccaag ttggggataa agtcccctat gacatctgcc ggccagacca
     3601 ctcagtgttg accctgcagc tgcctgtgac agcctccgtg agagaggtga tggcagcgtt
     3661 ggcccaggag gatggctgga ccaaggggca ggtgctggtg aaggtcaatt ctgcaggtga
     3721 tgccattggc ctgcagccag atgcccgtgg tgtggccaca tctctggggc tcaatgagcg
     3781 tctctttgtt gtcaacccac aggaagcgca tgagctgatc ccacaccctg accagctggg
     3841 gcccactgtg ggctctgctg aggggctgga cctggtgagt gccaaggacc tggcaggcca
     3901 gctgacggac cacgactgga gcctcttcaa cagtatccac caggtggagc tgatccacta
     3961 tgtgctgggc ccccagcatc tgcgggatgt caccaccgcc aacctggagc gcttcatgcg
     4021 ccgcttcaat gagctgcagt actgggtggc caccgagctg tgtctctgcc ccgtgcccgg
     4081 cccccgggcc cagctgctca ggaagttcat taagctggcg gcccacctca aggagcagaa
     4141 gaatctcaat tccttctttg ccgtcatgtt tggcctcagc aactcggcca tcagccgcct
     4201 agcccacacc tgggagcggc tgccccacaa agtccggaag ctgtactccg ccctcgagag
     4261 gctgctggat ccctcatgga accaccgggt ataccgactg gccctcgcca agctctcccc
     4321 tcctgtcatc cccttcatgc cccttcttgc agcagacatg gccgccattc atgagggaaa
     4381 ccacacacta gtggagaatc tcatcaactt tgagaagatg agaatgatgg ccagagccgc
     4441 gcggatgctg caccactgcc gaagccacaa ccctgtgcct ctctcaccac tcagaagccg
     4501 agtttcccac ctccacgagg acagccaggt ggcgaggatt tccacatgct cggagcagtc
     4561 cctgagcacc cggagtccag ccagcacctg ggcttatgtc cagcagctga aggtcattga
     4621 caaccagcgg gaactctccc gcctctcccg agagctggag ccagctagcg agctcatgga
     4681 cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     4741 gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga
     4801 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     4861 ggacgagctg tacaagggtg gcagcggtgg catggtgagc aagggcgagg agctgttcac
     4921 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     4981 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagc tgatctgcac
     5041 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgggct acggcctgca
     5101 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     5161 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     5221 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     5281 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     5341 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca
     5401 caacatcgag ccgaattttg tcttcctgat cggcgccgca ggaatactct tcgtatctag
     5461 cgagctcatg gacggcggcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     5521 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa
     5581 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     5641 cactctcggc atggacgagc tgtacaaggg tggcagcggt ggcatggtga gcaagggcga
     5701 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     5761 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     5821 gctgatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctggg
     5881 ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa
     5941 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     6001 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     6061 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     6121 caacagccac aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt
     6181 caagatccgc cacaacatcg agccgaattc cctcgaggtt aacgcggcgc gcctctaatc
     6241 taggggatcc accggaagcc acctgcatgc ggccgcatga tcagctggat gcatcgatca
     6301 cgcgtaccgg tgctcgaggt accgatgaat agctaaggtc gaggccgcag gtaagtatca
     6361 aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac
     6421 tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca
     6481 caggtgtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     6541 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     6601 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     6661 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     6721 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     6781 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     6841 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     6901 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     6961 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     7021 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     7081 ctggaattcg agctcggtac gatcagcctc gactgtgcct tctagttgcc agccatctgt
     7141 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     7201 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     7261 tggggtgggg caggacagca agggggagga ttgggaagac aatagcccag cttttgttcc
     7321 ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga
     7381 aattgttatc cgctaattca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg
     7441 tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta
     7501 tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt
     7561 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
     7621 aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat
     7681 gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc
     7741 tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt
     7801 ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc
     7861 cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag
     7921 atccctcgac ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     7981 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     8041 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     8101 agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa ccatagtccc
     8161 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     8221 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     8281 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc taacttgttt
     8341 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     8401 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     8461 tggatccgct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     8521 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     8581 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     8641 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     8701 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     8761 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     8821 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     8881 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     8941 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     9001 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     9061 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     9121 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     9181 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     9241 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     9301 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     9361 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     9421 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     9481 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     9541 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     9601 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     9661 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     9721 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     9781 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     9841 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     9901 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     9961 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
    10021 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
    10081 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    10141 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    10201 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    10261 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    10321 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    10381 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    10441 gaaaagtgcc acctgg
//
