LOCUS       sequence-356573-        5538 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Recombinant protein synthesis of human HSP90AB1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5538)
  AUTHORS   Ayoub CA, Wagner CS, Kuret J
  TITLE     Identification of gene networks mediating regional resistance to 
            tauopathy in late-onset Alzheimer's disease.
  JOURNAL   PLoS Genet. 2023 Mar 27;19(3):e1010681. doi: 
            10.1371/journal.pgen.1010681. eCollection 2023 Mar.
  PUBMED    36972319
REFERENCE   2  (bases 1 to 5538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2023-03-27"; volume: "19(3):e1010681. doi"; pages: "
            10.1371/journal.pgen.1010681. eCollection 2023 Mar"
COMMENT     Sequence Label: pT7C-HSP90AB1
FEATURES             Location/Qualifiers
     source          1..5538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             51..73
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             92..109
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             119..136
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     rep_origin      complement(2418..2930)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2612..2633)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2824..2843
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(2957..2975)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3043..3147
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3148..3216,3217..4008)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3366..3385)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4179..4767
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4668..4687
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4921..4938
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4953..5092
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5018..5037)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5178..5200
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5436..5455
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac
       61 tttaagaagg agatatacca tgggcagcag ccatcatcat catcatcaca gcagcggcct
      121 ggtgccgcgc ggcagccata tgactagtat gcctgaggaa gtgcaccatg gagaggagga
      181 ggtggagact tttgcctttc aggcagaaat tgcccaactc atgtccctca tcatcaatac
      241 cttctattcc aacaaggaga ttttccttcg ggagttgatc tctaatgctt ctgatgcctt
      301 ggacaagatt cgctatgaga gcctgacaga cccttcgaag ttggacagtg gtaaagagct
      361 gaaaattgac atcatcccca accctcagga acgtaccctg actttggtag acacaggcat
      421 tggcatgacc aaagctgatc tcataaataa tttgggaacc attgccaagt ctggtactaa
      481 agcattcatg gaggctcttc aggctggtgc agacatctcc atgattgggc agtttggtgt
      541 tggcttttat tctgcctact tggtggcaga gaaagtggtt gtgatcacaa agcacaacga
      601 tgatgaacag tatgcttggg agtcttctgc tggaggttcc ttcactgtgc gtgctgacca
      661 tggtgagccc attggcaggg gtaccaaagt gatcctccat cttaaagaag atcagacaga
      721 gtacctagaa gagaggcggg tcaaagaagt agtgaagaag cattctcagt tcataggcta
      781 tcccatcacc ctttatttgg agaaggaacg agagaaggaa attagtgatg atgaggcaga
      841 ggaagagaaa ggtgagaaag aagaggaaga taaagatgat gaagaaaagc ccaagatcga
      901 agatgtgggt tcagatgagg aggatgacag cggtaaggat aagaagaaga aaactaagaa
      961 gatcaaagag aaatacattg atcaggaaga actaaacaag accaagccta tttggaccag
     1021 aaaccctgat gacatcaccc aagaggagta tggagaattc tacaagagcc tcactaatga
     1081 ctgggaagac cacttggcag tcaagcactt ttctgtagaa ggtcagttgg aattcagggc
     1141 attgctattt attcctcgtc gggctccctt tgaccttttt gagaacaaga agaaaaagaa
     1201 caacatcaaa ctctatgtcc gccgtgtgtt catcatggac agctgtgatg agttgatacc
     1261 agagtatctc aattttatcc gtggtgtggt tgactctgag gatctgcccc tgaacatctc
     1321 ccgagaaatg ctccagcaga gcaaaatctt gaaagtcatt cgcaaaaaca ttgttaagaa
     1381 gtgccttgag ctcttctctg agctggcaga agacaaggag aattacaaga aattctatga
     1441 ggcattctct aaaaatctca agcttggaat ccacgaagac tccactaacc gccgccgcct
     1501 gtctgagctg ctgcgctatc acacctccca gtctggagat gagatgacat ctctgtcaga
     1561 gtatgtttct cgcatgaagg agacacagaa gtccatctat tacatcactg gtgagagcaa
     1621 agagcaggtg gccaactcag cttttgtgga gcgagtgcgg aaacggggct tcgaggtggt
     1681 atatatgacc gagcccattg acgagtactg tgtgcagcag ctcaaggaat ttgatgggaa
     1741 gagcctggtc tcagttacca aggagggtct ggagctgcct gaggatgagg aggagaagaa
     1801 gaagatggaa gagagcaagg caaagtttga gaacctctgc aagctcatga aagaaatctt
     1861 agataagaag gttgagaagg tgacaatctc caatagactt gtgtcttcac cttgctgcat
     1921 tgtgaccagc acctacggct ggacagccaa tatggagcgg atcatgaaag cccaggcact
     1981 tcgggacaac tccaccatgg gctatatgat ggccaaaaag cacctggaga tcaaccctga
     2041 ccaccccatt gtggagacgc tgcggcagaa ggctgaggcc gacaagaatg ataaggcagt
     2101 taaggacctg gtggtgctgc tgtttgaaac cgccctgcta tcttctggct tttcccttga
     2161 ggatccccag acccactcca accgcatcta tcgcatgatc aagctaggtc taggtattga
     2221 tgaagatgaa gtggcagcag aggaacccaa tgctgcagtt cctgatgaga tcccccctct
     2281 cgagggcgat gaggatgcgt ctcgcatgga agaagtcgat tagaagggcg aattctgcag
     2341 atatccatca cactggcggc cgctcgagtg atccggctgc taacaaagcc cgaaaggaag
     2401 ctgagttggc tgctgccacc ccggttgata atcagaaaag ccccaaaaac aggaagattg
     2461 tataagcaaa tatttaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     2521 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     2581 aagaatagac cgagataggg ttgagtttgt tccagtttgg aacaagagtc cactattaaa
     2641 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     2701 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     2761 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     2821 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     2881 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ggggatcaat
     2941 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     3001 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     3061 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     3121 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     3181 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     3241 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     3301 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     3361 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     3421 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     3481 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     3541 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     3601 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     3661 ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta
     3721 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     3781 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     3841 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     3901 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     3961 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     4021 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     4081 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     4141 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     4201 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     4261 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     4321 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     4381 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     4441 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     4501 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     4561 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     4621 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     4681 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     4741 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     4801 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     4861 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     4921 agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat
     4981 ctgtgcggta tttcacaccg caatggtgca ctctcagtac aatctgctct gatgccgcat
     5041 agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca
     5101 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     5161 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     5221 acgcgcgagg cagtcgatcg acgctctccc ttatgcgact cctgcattag gaagcagccc
     5281 agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg
     5341 gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc
     5401 atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca
     5461 gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag
     5521 atctcgatcc cgcgaaat
//
