LOCUS       sequence-356683-       10643 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human PD-1 extracellular domain and transmembrane 
            domain fused to BTLA intracellular domain followed by mEGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10643)
  AUTHORS   Xu X, Hou B, Fulzele A, Masubuchi T, Zhao Y, Wu Z, Hu Y, Jiang Y, Ma
            Y, Wang H, Bennett EJ, Fu G, Hui E
  TITLE     PD-1 and BTLA regulate T cell signaling differentially and only 
            partially through SHP1 and SHP2.
  JOURNAL   J Cell Biol. 2020 Jun 1;219(6). pii: 151801. doi: 
            10.1083/jcb.201905085.
  PUBMED    32437509
REFERENCE   2  (bases 1 to 10643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-06-1"; volume: "219(6). pii: 151801. doi"; 
            pages: " 10.1083/jcb.201905085"
COMMENT     Sequence Label: pHR-PD-1ecto&tmd-BTLAicd-mGFP
FEATURES             Location/Qualifiers
     source          1..10643
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(38..54)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(39..55)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             145..378
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(403..421)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(404..421)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(711..730)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(944..963)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1063..1085
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1123..1141)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1209..1313
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1314..1382,1383..2174)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1532..1551)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2345..2933
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2834..2853
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3087..3104
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3174..3194)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3179..3508
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3359..3494
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4642..4707
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4837..4857
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5282..5416
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5319..5338)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5373..5392
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5584..6217
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6264..6389
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6886..7119
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7304..7348
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7633..7750
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7903..8310
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      9304..9313
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9310..9312,9313..9315,9316..10029)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9355..9376)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9616..9635)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9963..9984
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(10090..10643,1..35)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10143..10163)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10561..10572)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagggaa
       61 ttaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
      121 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
      181 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
      241 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
      301 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
      361 tgtggaaaat ctctagcagc atctagaatt aattccgtgt attctatagt gtcacctaaa
      421 tcgtatgtgt atgatacata aggttatgta ttaattgtag ccgcgttcta acgacaatat
      481 gtacaagcct aattgtgtag catctggctt actgaagcag accctatcat ctctctcgta
      541 aactgccgtc agagtcggtt tggttggacg aaccttctga gtttctggta acgccgtccc
      601 gcacccggaa atggtcagcg aaccaatcag cagggtcatc gctagccaga tcctctacgc
      661 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc
      721 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg
      781 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc
      841 accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat
      901 gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc tgctctgatg
      961 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     1021 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     1081 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     1141 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     1201 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     1261 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     1321 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     1381 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     1441 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     1501 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     1561 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     1621 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     1681 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     1741 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     1801 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     1861 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     1921 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     1981 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     2041 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     2101 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     2161 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     2221 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     2281 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2341 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2401 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2461 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     2521 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     2581 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2641 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2701 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     2761 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     2821 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     2881 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     2941 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3001 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3061 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     3121 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt
     3181 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3241 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     3301 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     3361 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     3421 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     3481 cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat
     3541 gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact
     3601 catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc
     3661 cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat
     3721 acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc
     3781 cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt
     3841 actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca
     3901 cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat
     3961 cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc
     4021 aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta
     4081 tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt
     4141 cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct
     4201 ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga
     4261 cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga
     4321 cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta
     4381 aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg
     4441 acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg
     4501 acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg
     4561 ccccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct
     4621 acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta
     4681 ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag
     4741 tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat
     4801 gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa
     4861 gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat
     4921 agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag
     4981 aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac
     5041 atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa
     5101 aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat
     5161 agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt
     5221 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     5281 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     5341 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     5401 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
     5461 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
     5521 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
     5581 agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc
     5641 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
     5701 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
     5761 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
     5821 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
     5881 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt
     5941 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
     6001 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
     6061 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     6121 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     6181 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     6241 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     6301 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     6361 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     6421 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     6481 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     6541 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     6601 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     6661 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     6721 agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     6781 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     6841 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     6901 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     6961 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     7021 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     7081 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     7141 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     7201 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     7261 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     7321 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     7381 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     7441 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     7501 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     7561 agacagagac agatccattc gattagtgaa cggatctcga cggtatcgcc aaatggcagt
     7621 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     7681 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     7741 tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggatcg ataagcttga
     7801 tatcgaattc ctgcagcccc gataaaataa aagattttat ttagtctcca gaaaaagggg
     7861 ggaatgaaag accccacctg taggtttggc aagctagctg cagtaacgcc attttgcaag
     7921 gcatggaaaa ataccaaacc aagaatagag aagttcagat caagggcggg tacatgaaaa
     7981 tagctaacgt tgggccaaac aggatatctg cggtgagcag tttcggcccc ggcccggggc
     8041 caagaacaga tggtcaccgc agtttcggcc ccggcccgag gccaagaaca gatggtcccc
     8101 agatatggcc caaccctcag cagtttctta agacccatca gatgtttcca ggctccccca
     8161 aggacctgaa atgaccctgc gccttatttg aattaaccaa tcagcctgct tctcgcttct
     8221 gttcgcgcgc ttctgcttcc cgagctctat aaaagagctc acaacccctc actcggcgcg
     8281 ccagtcctcc gacagactga gtcgcccggg ggggatctgg agctctcgag aattctcatg
     8341 cagatcccac aggcgccctg gccagtcgtc tgggcggtgc tacaactggg ctggcggcca
     8401 ggatggttct tagactcccc agacaggccc tggaaccccc ccaccttctc cccagccctg
     8461 ctcgtggtga ccgaagggga caacgccacc ttcacctgca gcttctccaa cacatcggag
     8521 agcttcgtgc taaactggta ccgcatgagc cccagcaacc agacggacaa gctggccgct
     8581 ttccccgagg accgcagcca gcccggccag gactgccgct tccgtgtcac acaactgccc
     8641 aacgggcgtg acttccacat gagcgtggtc agggcccggc gcaatgacag cggcacctac
     8701 ctctgtgggg ccatctccct ggcccccaag gcgcagatca aagagagcct gcgggcagag
     8761 ctcagggtga cagagagaag ggcagaagtg cccacagccc accccagccc ctcacccagg
     8821 ccagccggcc agttccaaac cctggtggtt ggtgtcgtgg gcggcctgct gggcagcctg
     8881 gtgctgctag tctgggtcct ggccgtcatc tgctccagaa ggcaccaagg aaagcaaaat
     8941 gaactctctg acacagcagg aagggaaatt aacctggttg atgctcacct taagagtgag
     9001 caaacagaag caagcaccag gcaaaattcc caagtactgc tatcagaaac tggaatttat
     9061 gataatgacc ctgacctttg tttcaggatg caggaagggt ctgaagttta ttctaatcca
     9121 tgcctggaag aaaacaaacc aggcattgtt tatgcttccc tgaaccattc tgtcattgga
     9181 ctgaactcaa gactggcaag aaatgtaaaa gaagcaccaa cagaatatgc atccatatgt
     9241 gtgaggagta cgcgtctgca ggatatcaag cttgcggtac cgcgggcccg ggatccaccg
     9301 gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     9361 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     9421 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     9481 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     9541 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     9601 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     9661 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     9721 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     9781 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     9841 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     9901 gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga gaagcgcgat
     9961 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
    10021 tacaagtaaa gcggccgcga ctctagagtc gacctgcagg catgcaagct tgatatcaag
    10081 cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
    10141 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
    10201 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
    10261 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
    10321 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
    10381 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
    10441 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
    10501 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
    10561 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
    10621 ccgcgtcttc gccttcgccc tca
//
