LOCUS       sequence-356803-        7786 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mouse SHP1 fused to N-terminal mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7786)
  AUTHORS   Xu X, Hou B, Fulzele A, Masubuchi T, Zhao Y, Wu Z, Hu Y, Jiang Y, Ma
            Y, Wang H, Bennett EJ, Fu G, Hui E
  TITLE     PD-1 and BTLA regulate T cell signaling differentially and only 
            partially through SHP1 and SHP2.
  JOURNAL   J Cell Biol. 2020 Jun 1;219(6). pii: 151801. doi: 
            10.1083/jcb.201905085.
  PUBMED    32437509
REFERENCE   2  (bases 1 to 7786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-06-1"; volume: "219(6). pii: 151801. doi"; 
            pages: " 10.1083/jcb.201905085"
COMMENT     Sequence Label: pMIG II SIN-SV40-mCherry-SHP1
FEATURES             Location/Qualifiers
     source          1..7786
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      54..189
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             215..922
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(360..378)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     628..647
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    3411..3427
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3435..3441,3442..3459,3460..3465))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3480..3501
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3618..3635)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3789..4377)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3869..3888)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4548..5339,5340..5408))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5171..5190
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5409..5513)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5581..5599
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5637..5659)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5759..5778
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5972..5994
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6122..6141
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             6341..6857
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    6921..7262
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             7329..7745
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     7675..7697
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     7713..7729
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        join(7771..7786,1..195)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
ORIGIN
        1 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
       61 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
      121 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
      181 ttggaggcct aggctctcga gaattctcac gcgtatggtg agcaagggcg aggaggataa
      241 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
      301 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
      361 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
      421 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
      481 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
      541 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
      601 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
      661 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
      721 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
      781 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
      841 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
      901 cggcatggac gagctgtaca agacgcgtgg gagttccggt ggtggcggga gcggaggtgg
      961 aggctcgagc ggtggcggcc gcatggtgag gtggtttcac cgggacctca gcgggcctga
     1021 tgcagagacc ctgctgaagg gccggggagt ccctgggagc ttcctggctc ggcccagccg
     1081 caagaaccag ggtgacttct ccctctcagt cagggtggat gatcaggtga ctcatattcg
     1141 gatccagaac tcaggggact tctatgacct gtacggaggg gagaagtttg cgacgctgac
     1201 agagctggtc gagtattaca cgcagcagca gggcatcctg caggaccgag atggcaccat
     1261 catccacctt aagtacccac tgaactgctc ggaccccacc agtgagaggt ggtaccacgg
     1321 ccacatatct ggagggcagg cggagtcact gctgcaggcc aagggcgagc cctggacatt
     1381 tcttgtgcgt gagagtctca gccaacctgg tgattttgtg ctctctgtgc tcaatgacca
     1441 gcccaaggct ggcccaggtt ccccgctcag ggtcactcat atcaaggtta tgtgtgaggg
     1501 tggacgctat actgtgggtg gctcagagac gtttgacagc ctcacagacc tggtggagca
     1561 cttcaagaag acagggattg aggaggcctc gggtgccttt gtctacctgc ggcagcctta
     1621 ctacgctact cgggtaaacg cagctgacat tgagaatcgg gtcttggaac tgaacaagaa
     1681 gcaggagtcg gaggacacag ccaaggctgg cttctgggag gagtttgaga gtctacaaaa
     1741 gcaggaggta aagaatctac accaacgtct ggaagggcag cggccagaga acaagagcaa
     1801 gaaccgctac aagaacattc ttccctttga ccacagccga gtgatcctgc agggacgtga
     1861 cagtaacatc ccaggctctg actacatcaa tgccaactac gtgaagaacc agctgctagg
     1921 tccagatgag aactctaaga cctacatcgc cagccagggc tgtctggatg ccacagtcaa
     1981 tgacttctgg cagatggctt ggcaggagaa cactcgtgtc atcgtcatga ctaccagaga
     2041 ggtggagaaa ggccggaaca aatgtgtccc atactggccc gaggtgggca ctcagcgtgt
     2101 ctatggtctc tactctgtga ccaacagtag ggaacatgac acagcagaat acaaactgcg
     2161 aacattacag atctccccac tagacaatgg ggacctggtt cgggagatat ggcactacca
     2221 gtacctgagc tggcctgacc atggggttcc cagtgagcct gggggtgtcc tcagctttct
     2281 ggatcagatc aaccagcgac aggaaagttt gcctcatgca gggcccatca ttgtgcattg
     2341 cagcgctggc atcggccgca cgggcaccat catcgtcatt gatatgctta tggaaagcat
     2401 ctccaccaag gggctagact gtgacattga tatccagaag accatccaga tggtacgagc
     2461 acagcgctcc ggcatggtgc agaccgaggc ccagtacaag tttatttacg tggccattgc
     2521 ccagttcatc gaaacgacca agaagaaact ggagatcata caatcccaga agggccagga
     2581 gtcggagtat gggaatatca cgtaccctcc cgctgtgagg agtgcccacg ccaaagcctc
     2641 gcgtacttcc tccaagcaca aggaggaggt gtacgaaaac gtgcatagca agagcaagaa
     2701 ggaagagaaa gtaaagaagc agcggtcggc agacaaggag aagaacaaag gttctctcaa
     2761 gaggaagtga gcggccgcga ctctagagtc gacctgcagg catgcaagct tcaggtagcc
     2821 ggctaacgtt aacaaccggt acctctagaa ctatagctag catgcgcaaa tttaaagcgc
     2881 tgatatcgat aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc
     2941 ccacctgtag gtttggcaag aaccatcaga tgtttccagg gtgccccaag gacctgaaat
     3001 gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt
     3061 ctgctccccg agctcaataa aagagcccac aacccctcac tcggcgcgcc agtcctccga
     3121 tagactgcgt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact
     3181 tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg
     3241 gtctttcatg ggtaacagtt tcttgaagtt ggagaacaac attctgaggg taggagtcga
     3301 atattaagta atcctgactc aattagccac tgttttgaat ccacatactc caatactcct
     3361 gaaatagttc attatggaca gcgcagaaag agctggggag aattgtgaaa ttgttatccg
     3421 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     3481 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     3541 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     3601 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     3661 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     3721 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     3781 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     3841 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     3901 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     3961 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     4021 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     4081 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     4141 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     4201 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     4261 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     4321 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     4381 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     4441 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     4501 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     4561 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     4621 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     4681 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     4741 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     4801 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     4861 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     4921 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     4981 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     5041 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     5101 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     5161 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     5221 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     5281 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     5341 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     5401 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     5461 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     5521 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     5581 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     5641 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     5701 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     5761 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     5821 gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa
     5881 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     5941 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcg
     6001 caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca
     6061 ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat
     6121 cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca
     6181 cgatgcgtcc ggcgtagagg cgattagtcc aatttgttaa agacaggata tcagtggtcc
     6241 aggctctagt tttgactcaa caatatcacc agctgaagcc tatagagtac gagccataga
     6301 taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta
     6361 ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaata cataactgag
     6421 aatagagaag ttcagatcaa ggttaggaac agagagacag cagaatatgg gccaaacagg
     6481 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg
     6541 cggtcccgcc ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac
     6601 ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg
     6661 cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg gcgcgccagt
     6721 cctccgatag actgcgtcgc ccgggtaccc gtattcccaa taaagcctct tgctgtttgc
     6781 atccgaatcg tggactcgct gatccttggg agggtctcct cagattgatt gactgcccac
     6841 ctcgggggtc tttcatttgg aggttccacc gagatttgga gacccctgcc cagggaccac
     6901 cgaccccccc gccgggaggt aagctggcca gcggtcgttt cgtgtctgtc tctgtctttg
     6961 tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct gtactagtta gctaactagc
     7021 tctgtatctg gcggacccgt ggtggaactg acgagttctg aacacccggc cgcaaccctg
     7081 ggagacgtcc cagggacttt gggggccgtt tttgtggccc gacctgagga agggagtcga
     7141 tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg agacgagaac ctaaaacagt
     7201 tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg aagccgcgcg tcttgtctgc
     7261 tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac tgtgtttctg tatttgtctg
     7321 aaaattaggg ccagactgtt accactccct taagtttgac cttaggtcac tggaaagatg
     7381 tcgagcggat cgctcacaac cagtcggtag atgtcaagaa gagacgttgg gttaccttct
     7441 gctctgcaga atggccaacc tttaacgtcg gatggccgcg agacggcacc tttaaccgag
     7501 acctcatcac ccaggttaag atcaaggtct tttcacctgg cccgcatgga cacccagacc
     7561 aggtccccta catcgtgacc tgggaagcct tggcttttga cccccctccc tgggtcaagc
     7621 cctttgtaca ccctaagcct ccgcctcctc ttcctccatc cgccccgtct ctcccccttg
     7681 aacctcctcg ttcgaccccg cctcgatcct ccctttatcc agccctcact ccttctctag
     7741 gcgccggaat tcgatctgag ctctcgagag gcaggcagaa gtatgc
//
