LOCUS       sequence-356899-        2309 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For cloning Neisseria lactamica Type I-C crRNA for protein 
            purification in E.Coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Tan R, Krueger RK, Gramelspacher MJ, Zhou X, Xiao Y, Ke A, Hou Z, 
            Zhang Y
  TITLE     Cas11 enables genome engineering in human cells with compact 
            CRISPR-Cas3 systems.
  JOURNAL   Mol Cell. 2022 Feb 17;82(4):852-867.e5. doi: 
            10.1016/j.molcel.2021.12.032. Epub 2022 Jan 19.
  PUBMED    35051351
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.12.032"; journalName: "Mol Cell"; date: 
            "2022-02-17- 17"; volume: "82"; issue: "4"; pages: "852-867.e5"
COMMENT     Sequence Label: pACYC-T7-Nla-repeat-BbsI
FEATURES             Location/Qualifiers
     source          1..2309
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(364..1023)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     938..957
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1024..1126)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(1518..1535)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1652..2197)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1732..1751)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 attaatacga ctcactatag tcagccgcct ctaggcggct gtgtgttgaa acgggtcttc
       61 attattccag aagacaatca gccgcctcta ggcggctgtg tgttgaaact tttttttcta
      121 gatatcgctc aatactgacc atgctgaaac ctcaggcatt tgagaagcac acggtcacac
      181 tgcttccggt agtcaataaa ccggtaaacc agcaatagac ataagcggct atttaacgac
      241 cctgccctga accgacgacc gggtcgaatt tgctttcgaa tttctgccat tcatccgctt
      301 attatcactt attcaggcgt agcaaccagg cgtttaaggg caccaataac tgccttaaaa
      361 aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc
      421 gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt
      481 gtcgccttgc gtataatatt tgcccatagt gaaaacgggg gcgaagaagt tgtccatatt
      541 ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat
      601 attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg
      661 cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa
      721 cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag
      781 ctcaccgtct ttcattgcca tacggaactc cggatgagca ttcatcaggc gggcaagaat
      841 gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt
      901 aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa
      961 atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc
     1021 cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga
     1081 tcttatttca ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt ctcattttcg
     1141 ccaaaagttg gcccagggct tcccggtatc aacagggaca ccaggattta tttattctgc
     1201 gaagtgatct tccgtcacag gtatttattc ggcgcaaagt gcgtcgggtg atgctgccaa
     1261 cttactgatt tagtgtatga tggtgttttt gaggtgctcc agtggcttct gtttctatca
     1321 gctgtccctc ctgttcagct actgacgggg tggtgcgtaa cggcaaaagc accgccggac
     1381 atcagcgcta gcggagtgta tactggctta ctatgttggc actgatgagg gtgtcagtga
     1441 agtgcttcat gtggcaggag aaaaaaggct gcaccggtgc gtcagcagaa tatgtgatac
     1501 aggatatatt ccgcttcctc gctcactgac tcgctacgct cggtcgttcg actgcggcga
     1561 gcggaaatgg cttacgaacg gggcggagat ttcctggaag atgccaggaa gatacttaac
     1621 agggaagtga gagggccgcg gcaaagccgt ttttccatag gctccgcccc cctgacaagc
     1681 atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc gacaggacta taaagatacc
     1741 aggcgtttcc ccctggcggc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg
     1801 gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac gcctgacact cagttccggg
     1861 taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc
     1921 gccttatccg gtaactatcg tcttgagtcc aacccggaaa gacatgcaaa agcaccactg
     1981 gcagcagcca ctggtaattg atttagagga gttagtcttg aagtcatgcg ccggttaagg
     2041 ctaaactgaa aggacaagtt ttggtgactg cgctcctcca agccagttac ctcggttcaa
     2101 agagttggta gctcagagaa ccttcgaaaa accgccctgc aaggcggttt tttcgttttc
     2161 agagcaagag attacgcgca gaccaaaacg atctcaagaa gatcatctta ttaatcagat
     2221 aaaatatttc tagatttcag tgcaatttat ctcttcaaat gtagcacctg aagtcagccc
     2281 catacgatat aagttgtaat tctcatgtt
//
