LOCUS       sequence-356925-        4320 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV vector backbone containing a 1.3CaMKIIa promoter followed by a 
            multiple cloning site(MCS) followed by a poly-Adenylation Signal 
            (pA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Holehonnur R, Lella SK, Ho A, Luong JA, Ploski JE
  TITLE     The production of viral vectors designed to express large and 
            difficult to express transgenes within neurons.
  JOURNAL   Mol Brain. 2015 Feb 24;8:12. doi: 10.1186/s13041-015-0100-7.
  PUBMED    25887710
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Brain."; date: "2015-02-24"; volume: "8:12. doi"; pages: " 
            10.1186/s13041-015-0100-7"
COMMENT     Sequence Label: pJEP206-AAV-1.3CaMKII-MCS-pA.
FEATURES             Location/Qualifiers
     source          1..4320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        151..1438
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     primer_bind     complement(1525..1544)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   1583..1723
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   1583..1712
                     /label=AAV2 ITR
     rep_origin      1798..2253
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1885..1904)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2095..2116
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2270..2289)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2389..2411
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2449..2467)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2535..2639
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2640..2708,2709..3500)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2858..2877)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3671..4259
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4160..4179
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttcgtaa cattatggcc ttaggtcact tcatctccat
      181 ggggttcttc ttctgatttt ctagaaaatg agatgggggt gcagagagct tcctcagtga
      241 cctgcccagg gtcacatcag aaatgtcaga gctagaactt gaactcagat tactaatctt
      301 aaattccatg ccttgggggc atgcaagtac gatatacaga aggagtgaac tcattagggc
      361 agatgaccaa tgagtttagg aaagaagagt ccagggcagg gtacatctac accacccgcc
      421 cagccctggg tgagtccagc cacgttcacc tcattatagt tgcctctctc cagtcctacc
      481 ttgacgggaa gcacaagcag aaactgggac aggagcccca ggagaccaaa tcttcatggt
      541 ccctctggga ggatgggtgg ggagagctgt ggcagaggcc tcaggagggg ccctgctgct
      601 cagtggtgac agataggggt gagaaagcag acagagtcat tccgtcagca ttctgggtct
      661 gtttggtact tcttctcacg ctaaggtggc ggtgtgatat gcacaatggc taaaaagcag
      721 ggagagctgg aaagaaacaa ggacagagac agaggccaag tcaaccagac caattcccag
      781 aggaagcaaa gaaaccatta cagagactac aagggggaag ggaaggagag atgaattagc
      841 ttcccctgta aaccttagaa cccagctgtt gccagggcaa cggggcaata cctgtctctt
      901 cagaggagat gaagttgcca gggtaactac atcctgtctt tctcaaggac catcccagaa
      961 tgtggcaccc actagccgtt accatagcaa ctgcctcttt gccccactta atcccatccc
     1021 gtctgttaaa agggccctat agttggaggt gggggaggta ggaagagcga tgatcacttg
     1081 tggactaagt ttgttcgcat ccccttctcc aaccccctca gtacatcacc ctgggggaac
     1141 agggtccact tgctcctggg cccacacagt cctgcagtat tgtgtatata aggccagggc
     1201 aaagaggagc aggttttaaa gtgaaaggca ggcaggtgtt ggggaggcag ttaccggggc
     1261 aacgggaaca gggcgtttcg gaggtggttg ccatggggac ctggatgctg acgaaggctc
     1321 gcgaggctgt gagcagccac agtgccctgc tcagaagccc caagctcgtc agtcaagccg
     1381 gttctccgtt tgcactcagg agcacgggca ggcgagtggc ccctagttct gggggcagga
     1441 attcaagctt gctcagccat atggatatcg catgcggatc cactagtctc gagtttattg
     1501 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     1561 tttcactgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg
     1621 cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc
     1681 cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat
     1741 tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg
     1801 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     1861 cacttgccag cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     1921 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     1981 ctttatggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat
     2041 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     2101 tcttgttcca aactggaaca acactcaact ctatctcggg ctattctttt gatttataag
     2161 ggattttgcc gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     2221 cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct
     2281 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     2341 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     2401 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     2461 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     2521 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     2581 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     2641 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     2701 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     2761 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     2821 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     2881 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     2941 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     3001 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     3061 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     3121 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     3181 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     3241 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     3301 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     3361 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     3421 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     3481 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     3541 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     3601 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     3661 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3721 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3781 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     3841 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3901 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     3961 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     4021 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     4081 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     4141 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4201 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4261 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
