LOCUS       sequence-356963-        9804 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ""label stable F-actin under Rac2 neutrophil-specific knockout 
            background"".
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9804)
  AUTHORS   Wang Y, Hsu AY, Walton EM, Park SJ, Syahirah R, Wang T, Zhou W, Ding
            C, Lemke AP, Zhang G, Tobin DM, Deng Q
  TITLE     A robust and flexible CRISPR/Cas9-based system for 
            neutrophil-specific gene inactivation in zebrafish.
  JOURNAL   J Cell Sci. 2021 Apr 15;134(8). pii: 237799. doi: 
            10.1242/jcs.258574. Epub 2021 Apr 22.
  PUBMED    33722979
REFERENCE   2  (bases 1 to 9804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2021-04-15"; volume: "134(8). pii: 237799. doi"; 
            pages: " 10.1242/jcs.258574. Epub 2021 Apr 22"
COMMENT     Sequence Label: Tol2-LyzC-Utrophin-GFP-U6ac-Rac2 guides
FEATURES             Location/Qualifiers
     source          1..9804
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(514..525,526..688)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1061..1077
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1097..1117
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3520..3544
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     3546..3562
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3572..3591
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3572..3590
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3620..3629
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4451..4460
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4457..4459,4460..4462,4463..5176)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4457..4459,4460..4462,4463..5173)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4763..4782)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5110..5131
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(5207..5225)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    complement(5254..5278)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5291..5307)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    5323..5457
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(6701..6721)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(6729..6745)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    6761..6895
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(7334..7521,7522..7533)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7567..7583)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7567..7583)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(7620..7638)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7659..7675)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7672..7694)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7683..7699
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7707..7713,7714..7731,7732..7737))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7752..7773
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7890..7907)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8061..8649)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8141..8160)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8820..9611,9612..9680))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9443..9462
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9681..9785)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 atgctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat
     1081 gattacgcca agctatcaac tttgtataga aaagttggct ccgaattcgc ccttaaaact
     1141 cgagagtttg acttgggttg attatagcaa atagtggaac acaaacagat tttgaaatat
     1201 tcttgcaaat ttatttatta aatccattca aatattgtca ttttttgtac tgtgtaattt
     1261 atattgattt gctcatacaa tatttacatt tcgcttaaac aatacactat tatttatatt
     1321 taaaaatagc tatatataaa tcaaatgtat agctcttttt tacacaatta atcaaagcta
     1381 aaatatttgt cacacaaata cagtaagcca cttcacaatg atatcttcaa aatctgtgct
     1441 tgactgaatc atttcattta ttgttttgta attcacatta taccttacgt tctccaggca
     1501 cagtttgaat tgttgataaa tgaagataat acatctgatt taattagtct cctttgttta
     1561 taataacttt aatctaattt gatagtaata attccagcaa tacatccatt acaataaaaa
     1621 tcgctttttt attttgcaca ttagtttgcc ggtatttgtt taaagtttca ccatactttt
     1681 aaatcatgtg ctatttgaaa agggctgtta tgactggaat taaatgtttt tttttatatc
     1741 agtgttttag attgaagtac aatatttata ggtaaaattc accagcatat gcttaaagat
     1801 agccatgctc caggtccttg tgtccttcta aggtctgttt gtggtttaag ctgtttgttg
     1861 acacacgctt gtcttctcca tttttctgtg aagttattgt cagtttattg cacactgggt
     1921 tgcattaagc tgcagttttt tcacaacagt tttccacatt gtaacagtca gtctccaaat
     1981 tattccatca gtcctcaaat ttagtcattt gaggccatga aaacacttaa aactacatga
     2041 aggtagtttg gcattaggtt ttagacttgt tcttgtttgg catattgcaa cctgtgtaaa
     2101 aatgatttga tttttatttt tttttttttt ttttcagaaa gaaacggtcc aaaaagagtt
     2161 atgtgaacat ttatccatgt gtttaatgtt ttttaaatta aaaaaaaaca tttttattaa
     2221 accacttgtg actaaacctt ttttagttat tctattttgt gacgagtctg aatggccaaa
     2281 gtactcttga atccaaaatc tttatagtaa aatgtttaat cttacttgca gtgttgggta
     2341 taatttgtaa caaagttgaa ttccatatct gctgagtgaa aaagtaaagt aagagattgc
     2401 tgtaaatatt taggttattt atttattaat acaatataga aagtaacaaa acacaatgta
     2461 aaaaatcaat aaagaaagta aatattaaaa gtaatgataa actaatgatg taaacaatat
     2521 tagcaacaaa gtttgtacaa ttcagggagg gtaaagaatt tcacctaata gtcaaactct
     2581 gtattaaata atataaaatc tttgttctta gtctgaagag gagactgtga gagaaagctt
     2641 actggttgtt aaattagaaa atttgtaact tgcagattaa aatgatttaa ataattttat
     2701 aaaggccatg tttgaaacaa atgtaaaaac ataataaatg ttaagaattt cagatattga
     2761 gattttacaa tttaaaaaaa gcaataaagt aatagcagag acctacactc ttaaagtaaa
     2821 ttacccaact ctgcttacta gtaacttttg gaatagttac tagtatccat tgaacaaata
     2881 ctagaatcta attaaattgt gtgagtgtcc aatgaaaaga ttcttgtatt taaaacagta
     2941 ccagtatcta tatatgtaca aaatttagtg ctagcaaaat gcaaataaat actactgcat
     3001 ataatggatt gcattgtaaa tcgactcgcc ataatacatt tatttcctat ttttactttt
     3061 aacaaaaaat aagttcactg agtatttcat gtcattaaat cgcattaaac ctcttgcatg
     3121 ctttatgtac actagtgcag ttcgctaaat gaccaccggt gtcactaata acacgatcgt
     3181 ttctgaattc tgcattaaac gccatttata cgttgctctt attagtaaac aatttctcca
     3241 tcatgagctc gaccagtatt gcatatttca tcctcttaaa tccagagttg tttcttttaa
     3301 tcactttctc ttcatgataa aagcagtttt tggttttcag aagtttacaa caaacccagt
     3361 cagttattgc ctcagcaaac actggagcaa ctgcacactt gtgaaaagct tcttgcgaaa
     3421 cacaaacctt tctctggact tataaataaa ggcattcagc agctccacca tacacctcca
     3481 ctggtcttga taaagggatc ctttaagggc gaattcgacc caagtttgta caaaaaagca
     3541 ggcttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcgaattggg
     3601 taccgggccc cccctcgagg ccaccatggc caagtatgga gaacatgaag ccagtcctga
     3661 caatgggcag aacgaattca gtgatatcat taagtccaga tctgatgaac acaatgacgt
     3721 acagaagaaa acctttacca aatggataaa tgctcgattt tcaaagagtg ggaaaccacc
     3781 catcaatgat atgttcacag acctcaaaga tggaaggaag ctattggatc ttctagaagg
     3841 cctcacagga acatcactgc caaaggaacg tggttccaca agggtacatg ccttaaataa
     3901 cgtcaacaga gtgctgcagg ttttacatca gaacaatgtg gaattagtga atataggggg
     3961 aaccgacatt gtggatggaa atcacaaact gactttgggg ttactttgga gcatcatttt
     4021 gcactggcag gtgaaagatg tcatgaagga tgtcatgtcg gacctgcagc agacgaacag
     4081 tgagaagatc ctgctcagct gggtgcgtca gaccaccagg ccctacagcc aagtcaacgt
     4141 cctcaacttc accaccagct ggacagatgg actcgccttt aatgctgtcc tccaccgaca
     4201 taaacctgat ctcttcagct gggataaagt tgtcaaaatg tcaccaattg agagacttga
     4261 acatgccttc agcaaggctc aaacttattt gggaattgaa aagctgttag atcctgaaga
     4321 tgttgccgtt cggcttcctg acaagaaatc cataattatg tatttaacat ctttgtttga
     4381 ggtgctacct cagcaagtca ccatagaccg aattctgcag tcgacggtac cgcgggcccg
     4441 tccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     4501 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     4561 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     4621 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     4681 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     4741 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     4801 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     4861 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     4921 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     4981 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     5041 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     5101 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     5161 cgagctgtac aagtaagcgg ccgcggtgga gctccagctt ttgttccctt tagtgagggt
     5221 taattgcgcg cttggcgtaa tcatggtcat agcacccagc tttcttgtac aaagttgaga
     5281 tctataattc actggccgtc gttttacggt accatcgatg atgatccaga catgataaga
     5341 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     5401 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     5461 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     5521 agcaagtaaa acctctacaa atgtggtatg gctgattatg atcctctaga tcccttgggt
     5581 agcagcgaag tcgaggcatt tctgtcctgg ctggtctaga ggtctctcag gtttaaaaaa
     5641 agcaccgact cggtgccact ttttcaagtt gataacggac tagccttatt taaacttgct
     5701 atgctgtttc cagcatagct cttaaacaca gggtctcgca ttggcccgaa ctaggagcct
     5761 ggagaactgc tatataaacc cgggacttct gggtatgttt ctgccatgtg gtaagtggat
     5821 aaacctactt attcaactca tttacctcaa acatgacagt aaacctcttc ggggcaaacc
     5881 tgcaaacgct taaaaccttc aaagtttgaa accagcagtt ttgtagaggt ttaccgtagt
     5941 atttttgaac tgtaaagcat atgcagagga agctggtttt aggcttgtgt gattggatga
     6001 gttcttctgt gacgtaactg atcttttgtc ctcgtgtcct tcgccttccc ccacaggcat
     6061 gcgcggacct tcccccgccg cagaccagag gacaacatgc tcagacgcca gcaaacggcc
     6121 catcgaaaca ttcacagact ctcggattgg agagaccggg cccgaggtct ctgactaaaa
     6181 aagcaccgac tcggtgccac tttttcaagt tgataacgga ctagccttat ttaaacttgc
     6241 tatgctgttt ccagcatagc tcttaaacgg gactctggga tacagcccga accaagagct
     6301 ggagggagag ctatatatac cagggacttc tgggtatgtt tttgggaggt ggtgagtgac
     6361 taaaccactt attcagctcc cttagatcaa gtctgaccct atatcatggt gacataaacc
     6421 tgcaaactga taaaacctga aggatctcaa atccagagtt tgtgtgaggg attaccgtgg
     6481 taattttcaa actgtaaagc atatgcaaaa ttatctggtc ttggcttgag tgattgggtg
     6541 tctcggtgtg atgcagggac gttttcagtg acgtgtcctt ctccctcccc cacaggcatg
     6601 cgcagaacat ttcccccctc cttgaagacc agaacaaaag acgccgagag caggaaactc
     6661 gtcttactga atgaccgagg ctggagaaat cgaccctagg caactttatt atacatagtt
     6721 gataattcac tggccgtcgt tttacggtac catcgatgat gatccagaca tgataagata
     6781 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     6841 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     6901 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     6961 caagtaaaac ctctacaaat gtggtatggc tgattatgat cctctagatc agatctgcga
     7021 agatacggcc acgggtgctc ttgatcctgt ggctgatttt ggactgtgct gctcgcagct
     7081 gctgatgaat cacatacttc ctccattttc ttccactgat tgactgttat aatttcccta
     7141 atttccaggt caaggtgctg tgcattgtgg taatagatgt gacatgacgt cacttccaaa
     7201 ggaccaatga acatgtctga ccaatttcat ataatgtgaa aacgattttc ataggcagaa
     7261 taaataacat ttaaattaaa ctgggcatca gcgcaattca attggtttgg taatagcaag
     7321 ggaaaataga atgaagtgat ctccaaaaaa taagtacttt ttgactgtaa ataaaattgt
     7381 aaggagtaaa aagtactttt ttttctaaaa aaatgtaatt aagtaaaagt aaaagtattg
     7441 atttttaatt gtactcaagt aaagtaaaaa tccccaaaaa taatacttaa gtacagtaat
     7501 caagtaaaat tactcaagta ctttacacct ctggttcttg accccctacc ttcagcaagc
     7561 ccagcagatc cactagttct agagcggccg ccaccgcggt ggagctccag cttttgttcc
     7621 ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga
     7681 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     7741 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     7801 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     7861 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     7921 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     7981 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     8041 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     8101 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8161 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     8221 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     8281 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     8341 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     8401 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     8461 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     8521 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     8581 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     8641 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     8701 tcacgttaag gcattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     8761 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     8821 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     8881 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     8941 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     9001 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     9061 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     9121 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     9181 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     9241 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     9301 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     9361 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     9421 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     9481 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     9541 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     9601 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     9661 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     9721 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     9781 ccgcgcacat ttccccgaaa agtg
//
