LOCUS       sequence-356964-        9282 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ""label active Rac; control for neutrophil-specific knockout"".
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9282)
  AUTHORS   Wang Y, Hsu AY, Walton EM, Park SJ, Syahirah R, Wang T, Zhou W, Ding
            C, Lemke AP, Zhang G, Tobin DM, Deng Q
  TITLE     A robust and flexible CRISPR/Cas9-based system for 
            neutrophil-specific gene inactivation in zebrafish.
  JOURNAL   J Cell Sci. 2021 Apr 15;134(8). pii: 237799. doi: 
            10.1242/jcs.258574. Epub 2021 Apr 22.
  PUBMED    33722979
REFERENCE   2  (bases 1 to 9282)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2021-04-15"; volume: "134(8). pii: 237799. doi"; 
            pages: " 10.1242/jcs.258574. Epub 2021 Apr 22"
COMMENT     Sequence Label: Tol2-LyzC-PBD-GFP-U6ac-ctrl guides
FEATURES             Location/Qualifiers
     source          1..9282
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(514..525,526..688)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1061..1077
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1097..1117
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3520..3544
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     3546..3562
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3572..3590
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(3657..3659,3660..3662,3663..4373)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3657..3659,3660..3662,3663..4373)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3963..3982)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4310..4331
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(4659..4677)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    complement(4706..4730)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4743..4759)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    4775..4909
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(6179..6199)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(6207..6223)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    6239..6373
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(6812..6999,7000..7011)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7045..7061)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7045..7061)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(7098..7116)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7137..7153)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7150..7172)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7161..7177
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7185..7191,7192..7209,7210..7215))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7230..7251
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7368..7385)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7539..8127)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7619..7638)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8298..9089,9090..9158))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8921..8940
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9159..9263)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 atgctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat
     1081 gattacgcca agctatcaac tttgtataga aaagttggct ccgaattcgc ccttaaaact
     1141 cgagagtttg acttgggttg attatagcaa atagtggaac acaaacagat tttgaaatat
     1201 tcttgcaaat ttatttatta aatccattca aatattgtca ttttttgtac tgtgtaattt
     1261 atattgattt gctcatacaa tatttacatt tcgcttaaac aatacactat tatttatatt
     1321 taaaaatagc tatatataaa tcaaatgtat agctcttttt tacacaatta atcaaagcta
     1381 aaatatttgt cacacaaata cagtaagcca cttcacaatg atatcttcaa aatctgtgct
     1441 tgactgaatc atttcattta ttgttttgta attcacatta taccttacgt tctccaggca
     1501 cagtttgaat tgttgataaa tgaagataat acatctgatt taattagtct cctttgttta
     1561 taataacttt aatctaattt gatagtaata attccagcaa tacatccatt acaataaaaa
     1621 tcgctttttt attttgcaca ttagtttgcc ggtatttgtt taaagtttca ccatactttt
     1681 aaatcatgtg ctatttgaaa agggctgtta tgactggaat taaatgtttt tttttatatc
     1741 agtgttttag attgaagtac aatatttata ggtaaaattc accagcatat gcttaaagat
     1801 agccatgctc caggtccttg tgtccttcta aggtctgttt gtggtttaag ctgtttgttg
     1861 acacacgctt gtcttctcca tttttctgtg aagttattgt cagtttattg cacactgggt
     1921 tgcattaagc tgcagttttt tcacaacagt tttccacatt gtaacagtca gtctccaaat
     1981 tattccatca gtcctcaaat ttagtcattt gaggccatga aaacacttaa aactacatga
     2041 aggtagtttg gcattaggtt ttagacttgt tcttgtttgg catattgcaa cctgtgtaaa
     2101 aatgatttga tttttatttt tttttttttt ttttcagaaa gaaacggtcc aaaaagagtt
     2161 atgtgaacat ttatccatgt gtttaatgtt ttttaaatta aaaaaaaaca tttttattaa
     2221 accacttgtg actaaacctt ttttagttat tctattttgt gacgagtctg aatggccaaa
     2281 gtactcttga atccaaaatc tttatagtaa aatgtttaat cttacttgca gtgttgggta
     2341 taatttgtaa caaagttgaa ttccatatct gctgagtgaa aaagtaaagt aagagattgc
     2401 tgtaaatatt taggttattt atttattaat acaatataga aagtaacaaa acacaatgta
     2461 aaaaatcaat aaagaaagta aatattaaaa gtaatgataa actaatgatg taaacaatat
     2521 tagcaacaaa gtttgtacaa ttcagggagg gtaaagaatt tcacctaata gtcaaactct
     2581 gtattaaata atataaaatc tttgttctta gtctgaagag gagactgtga gagaaagctt
     2641 actggttgtt aaattagaaa atttgtaact tgcagattaa aatgatttaa ataattttat
     2701 aaaggccatg tttgaaacaa atgtaaaaac ataataaatg ttaagaattt cagatattga
     2761 gattttacaa tttaaaaaaa gcaataaagt aatagcagag acctacactc ttaaagtaaa
     2821 ttacccaact ctgcttacta gtaacttttg gaatagttac tagtatccat tgaacaaata
     2881 ctagaatcta attaaattgt gtgagtgtcc aatgaaaaga ttcttgtatt taaaacagta
     2941 ccagtatcta tatatgtaca aaatttagtg ctagcaaaat gcaaataaat actactgcat
     3001 ataatggatt gcattgtaaa tcgactcgcc ataatacatt tatttcctat ttttactttt
     3061 aacaaaaaat aagttcactg agtatttcat gtcattaaat cgcattaaac ctcttgcatg
     3121 ctttatgtac actagtgcag ttcgctaaat gaccaccggt gtcactaata acacgatcgt
     3181 ttctgaattc tgcattaaac gccatttata cgttgctctt attagtaaac aatttctcca
     3241 tcatgagctc gaccagtatt gcatatttca tcctcttaaa tccagagttg tttcttttaa
     3301 tcactttctc ttcatgataa aagcagtttt tggttttcag aagtttacaa caaacccagt
     3361 cagttattgc ctcagcaaac actggagcaa ctgcacactt gtgaaaagct tcttgcgaaa
     3421 cacaaacctt tctctggact tataaataaa ggcattcagc agctccacca tacacctcca
     3481 ctggtcttga taaagggatc ctttaagggc gaattcgacc caagtttgta caaaaaagca
     3541 ggcttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcgaattggg
     3601 taccgggccc cccctcgaga atacaagcta cttgttcttt ttgcaggatc caaaccatgg
     3661 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     3721 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     3781 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     3841 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     3901 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     3961 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     4021 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     4081 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     4141 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     4201 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     4261 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     4321 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtccggaa
     4381 aagagcgccc agagatctct cttccttcag actttgagca tacgattcat gtgggttttg
     4441 atgcagtcac cggggaattc actggaattc ctgagcaatg ggcacgacta ctccaaacct
     4501 ccaacataac aaagctggaa cagaagaaga acccacaagc tgttcttgat gttctcaagt
     4561 tctacgactc caaagaaacg gtcaacaacc agaaatctag aactatagtg agtcgtatta
     4621 cgtagatcgc ggccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattgcg
     4681 cgcttggcgt aatcatggtc atagcaccca gctttcttgt acaaagttga gatctataat
     4741 tcactggccg tcgttttacg gtaccatcga tgatgatcca gacatgataa gatacattga
     4801 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     4861 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     4921 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
     4981 aaacctctac aaatgtggta tggctgatta tgatcctcta gatcccttgg gtagcagcga
     5041 agtcgaggca tttctgtcct ggctggtcta gaggtctctc aggtttaaaa aaagcaccga
     5101 ctcggtgcca ctttttcaag ttgataacgg actagcctta tttaaacttg ctatgctgtt
     5161 tccagcatag ctcttaaaca gagacggtcg acagtctgca gtgtcgtctc tcgaactagg
     5221 agcctggaga actgctatat aaacccggga cttctgggta tgtttctgcc atgtggtaag
     5281 tggataaacc tacttattca actcatttac ctcaaacatg acagtaaacc tcttcggggc
     5341 aaacctgcaa acgcttaaaa ccttcaaagt ttgaaaccag cagttttgta gaggtttacc
     5401 gtagtatttt tgaactgtaa agcatatgca gaggaagctg gttttaggct tgtgtgattg
     5461 gatgagttct tctgtgacgt aactgatctt ttgtcctcgt gtccttcgcc ttcccccaca
     5521 ggcatgcgcg gaccttcccc cgccgcagac cagaggacaa catgctcaga cgccagcaaa
     5581 cggcccatcg aaacattcac agactctcgg attggagaga ccgggcccga ggtctctgac
     5641 taaaaaagca ccgactcggt gccacttttt caagttgata acggactagc cttatttaaa
     5701 cttgctatgc tgtttccagc atagctctta aacagagacg gtcgacagtc tgcagtgtcg
     5761 tctctcgaac caagagctgg agggagagct atatatacca gggacttctg ggtatgtttt
     5821 tgggaggtgg tgagtgacta aaccacttat tcagctccct tagatcaagt ctgaccctat
     5881 atcatggtga cataaacctg caaactgata aaacctgaag gatctcaaat ccagagtttg
     5941 tgtgagggat taccgtggta attttcaaac tgtaaagcat atgcaaaatt atctggtctt
     6001 ggcttgagtg attgggtgtc tcggtgtgat gcagggacgt tttcagtgac gtgtccttct
     6061 ccctccccca caggcatgcg cagaacattt cccccctcct tgaagaccag aacaaaagac
     6121 gccgagagca ggaaactcgt cttactgaat gaccgaggct ggagaaatcg accctaggca
     6181 actttattat acatagttga taattcactg gccgtcgttt tacggtacca tcgatgatga
     6241 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     6301 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     6361 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     6421 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatcc
     6481 tctagatcag atctgcgaag atacggccac gggtgctctt gatcctgtgg ctgattttgg
     6541 actgtgctgc tcgcagctgc tgatgaatca catacttcct ccattttctt ccactgattg
     6601 actgttataa tttccctaat ttccaggtca aggtgctgtg cattgtggta atagatgtga
     6661 catgacgtca cttccaaagg accaatgaac atgtctgacc aatttcatat aatgtgaaaa
     6721 cgattttcat aggcagaata aataacattt aaattaaact gggcatcagc gcaattcaat
     6781 tggtttggta atagcaaggg aaaatagaat gaagtgatct ccaaaaaata agtacttttt
     6841 gactgtaaat aaaattgtaa ggagtaaaaa gtactttttt ttctaaaaaa atgtaattaa
     6901 gtaaaagtaa aagtattgat ttttaattgt actcaagtaa agtaaaaatc cccaaaaata
     6961 atacttaagt acagtaatca agtaaaatta ctcaagtact ttacacctct ggttcttgac
     7021 cccctacctt cagcaagccc agcagatcca ctagttctag agcggccgcc accgcggtgg
     7081 agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
     7141 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     7201 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     7261 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     7321 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     7381 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     7441 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     7501 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     7561 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     7621 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     7681 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     7741 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     7801 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7861 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     7921 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     7981 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     8041 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     8101 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     8161 gctcagtgga acgaaaactc acgttaaggc attttggtca tgagattatc aaaaaggatc
     8221 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     8281 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     8341 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     8401 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     8461 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     8521 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     8581 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     8641 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     8701 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     8761 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     8821 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     8881 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     8941 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     9001 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     9061 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     9121 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     9181 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     9241 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tg
//
