LOCUS       sequence-357003-       11865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLenti vector backbone designed to express GFP-GluN2B from a TRE3G 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11865)
  AUTHORS   Holehonnur R, Lella SK, Ho A, Luong JA, Ploski JE
  TITLE     The production of viral vectors designed to express large and 
            difficult to express transgenes within neurons.
  JOURNAL   Mol Brain. 2015 Feb 24;8:12. doi: 10.1186/s13041-015-0100-7.
  PUBMED    25887710
REFERENCE   2  (bases 1 to 11865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Brain."; date: "2015-02-24"; volume: "8:12. doi"; pages: " 
            10.1186/s13041-015-0100-7"
COMMENT     Sequence Label: pJEP203-Lenti-TRE3G-GFPGluN2B-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..11865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1950..2328
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    1958..1976
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1994..2012
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2030..2048
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2066..2084
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2102..2120
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2138..2156
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2174..2192
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2237..2261
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2363..2387
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(2495..2497,2498..3208)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(2495..2497,2498..3208)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2537..2558)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2798..2817)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3145..3166
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(7576..7600)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7653..7694
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7718..8306
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7771..7791)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8189..8200)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8386..8619
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8691..8812
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8728..8747)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8782..8801
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8852..8987
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9007..9026)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9008..9026)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9036..9053)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9036..9052)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9045..9067)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9194..9649
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9281..9300)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9491..9512
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9675..9779
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9780..9848,9849..10640)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9998..10017)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10811..11399
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11300..11319
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11553..11570
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11687..11708
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11723..11728,11729..11746,11747..11753)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11761..11777
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11766..11788
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11785..11801
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11785..11801
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11820..11840
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11822..11840
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatatc acgagactag cctcgagttg gctttactcc ctatcagtga tagagaacgt
     1981 atgaagagtt tactccctat cagtgataga gaacgtatgc agactttact ccctatcagt
     2041 gatagagaac gtataaggag tttactccct atcagtgata gagaacgtat gaccagttta
     2101 ctccctatca gtgatagaga acgtatctac agtttactcc ctatcagtga tagagaacgt
     2161 atatccagtt tactccctat cagtgataga gaacgtataa gctttaggcg tgtacggtgg
     2221 gcgcctataa aagcagagct cgtttagtga accgtcagat cgcctggagc aattccacaa
     2281 cacttttgtc ttataccaac tttccgtacc acttcctacc ctcgtaaaac tagtccagtg
     2341 tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gcaggctacg ccaccatgaa
     2401 gcccagcgca gagtgctgtt cccccaagtt ctggttggtg ttggccgtct tggccgtatc
     2461 aggcagcaaa gctcgttccc aaaagagcgc tagggtgagc aagggcgagg agctgttcac
     2521 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     2581 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     2641 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     2701 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     2761 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     2821 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     2881 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     2941 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     3001 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     3061 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     3121 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     3181 cactctcggc atggacgagc tgtacaaggc tagccccccc agcatcggca tcgctgtcat
     3241 cctcgtgggc acttcagacg aagtggccat aaaagacgcc cacgagaaag atgacttcca
     3301 tcatctctca gtagttcccc gggtggagct ggtagccatg aacgaaactg acccaaagag
     3361 catcatcacc cgtatctgcg atcttatgtc tgaccggaag atccaggggg tggtgttcgc
     3421 ggatgacacc gaccaagaag ccatcgctca gatcctcgac ttcatttctg ctcagactct
     3481 cacccccatc ctgggcatcc atgggggctc atctatgata atggcggata aggatgagtc
     3541 ctccatgttc ttccagtttg gcccgtctat cgaacagcaa gcttccgtca tgctcaacat
     3601 catggaagaa tatgactggt acatcttttc catcgtcacc acctacttcc ctggctacca
     3661 ggactttgtg aacaagatcc gcagtaccat cgagaacagc ttcgtgggct gggagctcga
     3721 ggaagtcctc ctgctagaca tgtctctgga cgatggcgac tctaagattc agaatcagct
     3781 gaagaagctc caaagcccca tcattctcct ttattgcacg aaggaggaag ccacctacat
     3841 ttttgaagta gctaactcag ttgggctgac tggctacggc tacacgtgga ttgtgccgag
     3901 tctggtggcc ggggatacgg acacggtgcc ttcagagttc cccacggggc ttatctctgt
     3961 gtcttatgat gaatgggact atggccttcc tgccagagtg agagatggaa ttgccatcat
     4021 caccactgct gcctcggaca tgctgtccga acacagtttc atccctgagc ccaagagcag
     4081 ttgctacaac acccacgaga agaggatcta ccagtctaac atgttgaata ggtatctgat
     4141 caatgtcact tttgaaggga gaaacctgtc cttcagcgaa gatggctacc agatgcatcc
     4201 gaagctggtg ataatccttc tgaacaagga gaggaagtgg gagagggtgg ggaaatggaa
     4261 ggacaagtcc ctgcagatga agtattatgt gtggcctcgg atgtgtcctg agactgagga
     4321 gcaagaggat gaccatctga gcattgtcac cttggaggag gcgccatttg tcattgtgga
     4381 aagcgtggac cctctcagtg gaacctgcat gaggaataca gtcccgtgcc agaagcgcat
     4441 catctctgag aataaaacag atgaggaacc aggctacatc aaaaaatgct gcaaggggtt
     4501 ctgtattgac atccttaaga aaatttctaa gtctgtgaag ttcacctatg acctttacct
     4561 ggtgaccaat ggcaagcacg ggaagaagat taatgggacc tggaatggca tgatcggtga
     4621 ggtggtcatg aagagggcct acatggcagt gggatcacta actatcaatg aagaacggtc
     4681 agaggtggtt gacttctctg tacccttcat agaaactggc atcagtgtca tggtatctcg
     4741 cagcaatggg actgtgtcac cttctgcctt cttagagcca ttcagcgctg acgtgtgggt
     4801 gatgatgttt gtgatgctgc tcattgtttc tgcggtggct gtctttgtct ttgaatactt
     4861 cagccctgtg ggttacaaca ggtgcctagc cgatggcaga gagccaggag gcccatcttt
     4921 caccatcggc aaagcaattt ggttactctg gggtctggtg tttaacaact ccgtacctgt
     4981 gcagaaccca aaggggacca cctccaagat catggtgtca gtgtgggcct tctttgctgt
     5041 cattttcctg gccagctaca ctgccaactt agcagccttc atgatccaag aggagtatgt
     5101 ggaccaggtt tctggcctga gtgacaagaa gttccagaga cctaatgact tctcaccccc
     5161 tttccgcttt gggactgtgc ccaatggcag cacagagagg aatatccgta ataactatgc
     5221 agaaatgcat gcctacatgg gaaagttcaa ccaaaggggt gtagatgatg cattgctctc
     5281 cctgaaaaca gggaagcttg atgcattcat ctatgatgca gctgtgctca actacatggc
     5341 tggaagggac gaaggctgca aactggtgac cattggcagt ggcaaggtct ttgcttctac
     5401 cggctatggc attgctatcc aaaaggactc cgggtggaag cgccaggtgg acctggctat
     5461 cctgcagctg tttggagatg gggagatgga agaactggaa gctctctggc tcactggcat
     5521 ttgccacaat gagaagaatg aggtgatgag cagccagctg gacatcgaca atatggcagg
     5581 tgtcttctat atgttggggg cagccatggc cctcagcctc atcaccttca tctgtgagca
     5641 tctgttctat tggcagttcc ggcattgctt catgggtgtc tgttctggca agcctggcat
     5701 ggtcttctcc atcagcagag gtatctacag ctgtatccat ggggtagcca tagaggagcg
     5761 ccaatccgtg atgaactccc ccactgccac catgaacaac acccactcca acatcctacg
     5821 cttgctccgc acggccaaga acatggccaa cctgtctgga gtaaacggct cccctcagag
     5881 tgccctggac ttcatccgcc gagagtcctc cgtctacgac atctctgagc atcgtcgcag
     5941 cttcacgcat tcagactgca agtcttacaa taacccaccc tgtgaggaaa acctgttcag
     6001 tgactacatt agcgaggtag agagaacatt tggtaacctg cagctgaagg acagcaatgt
     6061 gtaccaagac cactatcacc atcaccaccg gccacacagc atcggcagca ccagctccat
     6121 tgatgggctc tatgactgtg acaacccacc cttcaccacc cagcccaggt caatcagcaa
     6181 gaaacccctg gacatcggcc tgccctcctc caaacatagc cagctcagcg acctgtatgg
     6241 caagttctct ttcaagagtg accgctacag tggccacgac gacttgattc gatcggatgt
     6301 ctccgacatc tccacgcaca ctgtcaccta tgggaacatc gagggcaacg cagccaagag
     6361 gaggaaacag cagtataagg acagtctaaa gaagcggcca gcctcggcca aatcgaggag
     6421 ggagtttgat gaaatcgagc tggcctaccg tcgccgacca ccccgctccc cggaccacaa
     6481 gcgctacttc agggacaaag aagggctccg agacttctac ctggaccagt tccgaacaaa
     6541 ggagaactcg cctcactggg agcacgtgga cttgactgac atttacaaag aacgcagtga
     6601 cgacttcaag cgagattcgg tcagtggagg tgggccctgt accaacaggt ctcacctcaa
     6661 acacggaacg ggcgagaagc acggagtggt aggcggggtg cctgctcctt gggagaagaa
     6721 cctgaccaat gtggattggg aggaccggtc tgggggcaac ttctgccgca gctgtccttc
     6781 caagctgcac aattactcct cgacggtggc agggcagaac tcgggccggc aggcctgcat
     6841 cagatgtgag gcctgtaaga aggctggtaa cctgtacgac atcagcgagg acaactccct
     6901 gcaggaactg gaccagccgg ctgcccccgt ggctgtgaca tccaacgcct ccagcaccaa
     6961 gtaccctcaa agcccgacta attccaaggc tcagaagaag aatcggaaca aactgcgccg
     7021 gcagcattcc tacgacacct tcgtggacct gcagaaggag gaggccgcct tggccccacg
     7081 cagcgtgagc ctgaaagaca agggccgatt catggatggg agcccctacg cccatatgtt
     7141 tgagatgcca gctggtgaga gctcctttgc caacaagtcc tcagtgccca ctgccggaca
     7201 ccaccacaac aaccccggca gcggctacat gctcagcaag tcgctctacc ctgaccgggt
     7261 cacgcaaaac cctttcatcc ccacttttgg ggatgaccag tgcttgcttc acggcagcaa
     7321 atcctacttc ttcaggcagc ccacggtggc aggggcgtca aaaacaaggc cggacttccg
     7381 ggcccttgtc accaataagc cagtggtgtc agcccttcat ggggctgtgc caggtcgttt
     7441 ccagaaggac atttgtatag ggaaccagtc caacccctgt gtgcctaaca acaaaaaccc
     7501 cagggctttc aatggctcca gcaatggaca tgtttatgag aaactttcta gtattgagtc
     7561 tgatgtctga gtgagaccca gctttcttgt acaaagtggt tgatatccag cacagtggcg
     7621 gccgctcgag tctagagggc ccgcggttcg aaggtaagcc tatccctaac cctctcctcg
     7681 gtctcgattc tacgcgtacc ggttagtaat gatcgacaat caacctctgg attacaaaat
     7741 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     7801 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     7861 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     7921 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     7981 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     8041 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     8101 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     8161 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     8221 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     8281 gatctccctt tgggccgcct ccccgcctgg cgatggtacc tttaagacca atgacttaca
     8341 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     8401 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     8461 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     8521 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     8581 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     8641 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     8701 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     8761 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     8821 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     8881 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     8941 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     9001 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     9061 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     9121 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     9181 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     9241 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     9301 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     9361 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     9421 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     9481 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     9541 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     9601 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     9661 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     9721 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     9781 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     9841 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     9901 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     9961 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
    10021 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
    10081 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
    10141 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
    10201 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
    10261 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
    10321 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
    10381 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
    10441 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
    10501 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
    10561 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
    10621 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
    10681 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
    10741 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    10801 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    10861 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    10921 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
    10981 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    11041 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    11101 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    11161 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    11221 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    11281 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    11341 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    11401 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    11461 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    11521 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    11581 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
    11641 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
    11701 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
    11761 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
    11821 caattaaccc tcactaaagg gaacaaaagc tggagctgca agctt
//
