LOCUS       sequence-357009-        4893 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC26A4_STOP
FEATURES             Location/Qualifiers
     source          1..4893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2450..2549)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2566..2585)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2567..2585)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2590..2606)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2590..2606)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2708..2727)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2719..3528
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2786..2805)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3675..4263
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4164..4183
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4417..4434
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4593..4620)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4712..4798)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4847..4869
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4861..4878
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4862..4878
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggc agcaccagga
      121 ggcagaagcg agccacctca gctgcccgag tactcttgca gctatatggt gtctaggcca
      181 gtgtacagcg agctggcctt ccagcagcag cacgagcgga gactgcagga gaggaagacc
      241 ctgcgcgaga gcctggccaa gtgctgttcc tgttctagga agcgcgcctt tggcgtgctg
      301 aagacactgg tgcctatcct ggagtggctg ccaaagtatc gcgtgaagga gtggctgctg
      361 tccgacgtga tctctggcgt gagcaccgga ctggtggcca cactgcaggg aatggcatac
      421 gcactgctgg cagccgtgcc tgtgggctac ggcctgtatt ctgccttctt tccaatcctg
      481 acctatttca tctttggcac atctcggcac atcagcgtgg gccccttccc tgtggtgagc
      541 ctgatggtgg gctccgtggt gctgtctatg gcccccgacg agcactttct ggtgagctcc
      601 tctaacggca ccgtgctgaa taccacaatg atcgacaccg ccgcccggga tacagccaga
      661 gtgctgatcg cctccgccct gacactgctg gtgggcatca tccagctgat cttcggcggc
      721 ctgcagatcg gctttatcgt gaggtacctg gccgatcctc tggtgggagg cttcaccaca
      781 gcagcagcat ttcaggtgct ggtgtctcag ctgaagatcg tgctgaacgt gagcaccaag
      841 aactacaatg gcgtgctgtc catcatctat accctggtgg agatcttcca gaacatcggc
      901 gacacaaatc tggccgactt caccgccggc ctgctgacaa tcgtggtgtg catggccgtg
      961 aaggagctga acgaccggtt cagacacaag atcccagtgc ccatccctat cgaagtgatc
     1021 gtgaccatca tcgccacagc catctcctac ggcgccaatc tggagaagaa ctataatgcc
     1081 ggcatcgtga agtctatccc cagaggcttt ctgccacccg agctgcctcc agtgtccctg
     1141 ttctctgaga tgctggccgc cagcttttcc atcgcagtgg tggcatacgc aatcgccgtg
     1201 agcgtgggca aggtgtatgc caccaagtac gactatacaa tcgatggcaa ccaggagttc
     1261 atcgcctttg gcatcagcaa tatcttctcc ggcttctttt cttgttttgt ggcaaccaca
     1321 gccctgagca ggaccgcagt gcaggagtcc accggaggca agacacaggt ggcaggcatc
     1381 atcagcgccg ccatcgtgat gatcgccatc ctggccctgg gcaagctgct ggagcctctg
     1441 cagaagtccg tgctggcagc agtggtcatc gcaaacctga agggcatgtt catgcagctg
     1501 tgcgacatcc caaggctgtg gcgccagaat aagatcgatg ccgtgatctg ggtgtttacc
     1561 tgtatcgtgt ccatcatcct gggactggac ctgggactgc tggcaggact gatcttcggc
     1621 ctgctgacag tggtgctgag agtgcagttt ccctcttgga acggcctggg cagcatccct
     1681 tccaccgata tctacaagag cacaaagaac tataagaata tcgaggagcc ccagggcgtg
     1741 aagatcctga ggttcagctc ccctatcttt tacggcaacg tggacggctt caagaagtgc
     1801 atcaagtcca ccgtgggctt tgatgccatc agggtgtata ataagcgcct gaaggccctg
     1861 cggaagatcc agaagctgat caagtctggc cagctgcgcg ccaccaagaa tggcatcatc
     1921 agcgacgccg tgtccacaaa caatgccttc gagccagacg aggatatcga ggacctggag
     1981 gagctggata tccccaccaa ggagatcgag atccaggtgg attggaactc tgagctgcct
     2041 gtgaaagtga atgtgccaaa ggtgcccatc cactctctgg tgctggactg cggcgccatc
     2101 agcttcctgg atgtggtggg cgtgcggtcc ctgagagtga tcgtgaagga gttccagcgc
     2161 atcgacgtga acgtgtactt tgcctccctg caggattatg tgatcgagaa gctggagcag
     2221 tgtggcttct ttgacgataa catccggaag gacaccttct ttctgacagt gcacgatgcc
     2281 atcctgtacc tgcagaatca ggtgaagtcc caggagggcc agggctctat cctggagacc
     2341 atcacactga tccaggactg taaggatacc ctggagctga tcgagaccga gctgacagag
     2401 gaggagctgg acgtgcagga tgaggccatg cggacactgg ccagctaaga cccagctttc
     2461 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
     2521 actatcagtc aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg
     2581 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
     2641 tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac
     2701 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat
     2761 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     2821 ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     2881 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     2941 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     3001 ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca
     3061 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     3121 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     3181 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     3241 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat
     3301 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     3361 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     3421 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     3481 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt
     3541 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga
     3601 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag
     3661 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     3721 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     3781 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     3841 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     3901 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     3961 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     4021 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     4081 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     4141 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     4201 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     4261 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     4321 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     4381 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     4441 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     4501 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt
     4561 accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct
     4621 gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt
     4681 gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg
     4741 acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat
     4801 gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt
     4861 tgtaaaacga cggccagtct taagctcggg ccc
//
