LOCUS       sequence-357085-        4035 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EcoRV digestion produces 12x601 chromatin assembly construct with 20
            bp linker lengths in addition to carrier DNA that prevents 
            overassembly of nucleosomes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Gibson BA, Doolittle LK, Schneider MWG, Jensen LE, Gamarra N, Henry 
            L, Gerlich DW, Redding S, Rosen MK
  TITLE     Organization of Chromatin by Intrinsic and Regulated Phase 
            Separation.
  JOURNAL   Cell. 2019 Oct 3;179(2):470-484.e21. doi: 
            10.1016/j.cell.2019.08.037. Epub 2019 Sep 19.
  PUBMED    31543265
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.08.037"; journalName: "Cell"; date: "2019-10-3-
            3"; volume: "179"; issue: "2"; pages: "470-484.e21"
COMMENT     Sequence Label: pWM_12x601_20bpLinker
FEATURES             Location/Qualifiers
     source          1..4035
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(322..413)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2679..2695)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2679..2695)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2692..2714)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2703..2719
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2727..2733,2734..2751,2752..2757))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2772..2793
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(2834..3421)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2914..2933)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgtcactggg gagtattcaa ccaagtcatt ctgcgaatag gatatccgtc gtccgagttg
       61 ctcttgcccc gcatctacac gggataatac tgctccacat agcagaactt tgaaagtgct
      121 catcattgga aaacgctctt cagggcgaaa actctcaagg attttaccgc tattcaagtc
      181 cagttctatg tatcccactc gtgcccccaa ttggtcctct gcgtctttta ctttcacgag
      241 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      301 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      361 ttattgtctc atgagcggat atctatttga atgtatttag aaaaataaac aaatagggga
      421 attaaaaaaa agcccgctca ttaggcgggc tactagttta acgccattcg ccattcagac
      481 tacgcaactg ttgggaaggg cggtcagtgc gggcttcttc gctattacgc caactggcga
      541 aaggggggtg tgcggcaagg cgattaagtt gggtaacgcc aggattttcc cagtctcgtc
      601 gctgtaaaac gacggcaagc ccgaattcga gctcggtacc cggggatccg atatcatgat
      661 aactggagaa tcccggtgcc gaggccgctc aattggtcgt agacagctct agcaccgctt
      721 aaacgcacgt acgcgctgtc ccccgcgttt taaccgccaa ggggattact ccctagtctc
      781 caggcacgtg tcagatatat acatcctgtt catccagcta gctggataac tggagaatcc
      841 cggtgccgag gccgctcaat tggtcgtaga cagctctagc accgcttaaa cgcacgtacg
      901 cgctgtcccc cgcgttttaa ccgccaaggg gattactccc tagtctccag gcacgtgtca
      961 gatatataca tcctgttcat ccatctagct ggataactgg agaatcccgg tgccgaggcc
     1021 gctcaattgg tcgtagacag ctctagcacc gcttaaacgc acgtacgcgc tgtcccccgc
     1081 gttttaaccg ccaaggggat tactccctag tctccaggca cgtgtcagat atatacatcc
     1141 tgttcatcca tctagctgga taactggaga atcccggtgc cgaggccgct caattggtcg
     1201 tagacagctc tagcaccgct taaacgcacg tacgcgctgt cccccgcgtt ttaaccgcca
     1261 aggggattac tccctagtct ccaggcacgt gtcagatata tacatcctgt tcatccatct
     1321 agctggataa ctggagaatc ccggtgccga ggccgctcaa ttggtcgtag acagctctag
     1381 caccgcttaa acgcacgtac gcgctgtccc ccgcgtttta accgccaagg ggattactcc
     1441 ctagtctcca ggcacgtgtc agatatatac atcctgttca tccatctagc tggataactg
     1501 gagaatcccg gtgccgaggc cgctcaattg gtcgtagaca gctctagcac cgcttaaacg
     1561 cacgtacgcg ctgtcccccg cgttttaacc gccaagggga ttactcccta gtctccaggc
     1621 acgtgtcaga tatatacatc ctgttcatcc atctagctgg ataactggag aatcccggtg
     1681 ccgaggccgc tcaattggtc gtagacagct ctagcaccgc ttaaacgcac gtacgcgctg
     1741 tcccccgcgt tttaaccgcc aaggggatta ctccctagtc tccaggcacg tgtcagatat
     1801 atacatcctg ttcatccatc tagctggata actggagaat cccggtgccg aggccgctca
     1861 attggtcgta gacagctcta gcaccgctta aacgcacgta cgcgctgtcc cccgcgtttt
     1921 aaccgccaag gggattactc cctagtctcc aggcacgtgt cagatatata catcctgttc
     1981 atccatctag ctggataact ggagaatccc ggtgccgagg ccgctcaatt ggtcgtagac
     2041 agctctagca ccgcttaaac gcacgtacgc gctgtccccc gcgttttaac cgccaagggg
     2101 attactccct agtctccagg cacgtgtcag atatatacat cctgttcatc catctagctg
     2161 gataactgga gaatcccggt gccgaggccg ctcaattggt cgtagacagc tctagcaccg
     2221 cttaaacgca cgtacgcgct gtcccccgcg ttttaaccgc caaggggatt actccctagt
     2281 ctccaggcac gtgtcagata tatacatcct gttcatccat ctagctggat aactggagaa
     2341 tcccggtgcc gaggccgctc aattggtcgt agacagctct agcaccgctt aaacgcacgt
     2401 acgcgctgtc ccccgcgttt taaccgccaa ggggattact ccctagtctc caggcacgtg
     2461 tcagatatat acatcctgtt catccatcta gatggataac tggagaatcc cggtgccgag
     2521 gccgctcaat tggtcgtaga cagctctagc accgcttaaa cgcacgtacg cgctgtcccc
     2581 cgcgttttaa ccgccaaggg gattactccc tagtctccag gcacgtgtca gatatataca
     2641 tcctgttcat catgatatca agcttgggcg taatcatagt catagctgtt tcctgtgtga
     2701 aattgttatc cgctcacaat tccacacaac atacgagccg gcagcataaa gtgtaaagcc
     2761 tggggtgcct aatgagtgag ctaactcaca ttaatcaatt caggaaccgt aaaaaggccg
     2821 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2881 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     2941 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     3001 tcgatatcgg aacgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     3061 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     3121 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     3181 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     3241 gaagtggtgg ccgatatccg gctacactag aagaacagta tttggtatct gcgctctgct
     3301 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac ataccaccgc
     3361 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     3421 agaagatcct ttgatctttt ctactcggtc cgtccaaaaa aaaaggctcc aaaaggagcc
     3481 tttaatgata tcggtttacc aatgcttaat cagtgaagcc cctatttcag caatctgtct
     3541 atttcgttcg tccatagttg cctgactacc cgtcgtgtag ataactacga tacgggaagg
     3601 cttaccatct ggtccaagtg cggcaatgat accccgtgaa ccacgctctc ctgccccaga
     3661 tttatcagca ataaaccagc cagcaggaag tgccgaacgc agtagtggtc ctgcaacttt
     3721 atccgcttca agccagtcta ttaattgttg ccgtgaagcc agagtaagta gttcgccaga
     3781 tatcagtttg cgtaatgttg ttgccattgc cgcaggcatt gtggtgtctc gctcgtcgtt
     3841 tggtatggct tcattcagtt ctggttccca acggtcaagg cgagttacat ggtctcccat
     3901 attgtgcaaa aaagcggtta attccttcgg tccaccgatg gttgtcagaa gtaagttcgc
     3961 cgcagtgtta tcactcatcg ttatggcggc actacataat tctcttaccg tcattccgtc
     4021 cgtaaggtgc ttttc
//
