LOCUS       sequence-357218-        8564 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of Dm4E-T_1-58-DmeIF4Ga_687-1666.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8564)
  AUTHORS   Igreja C, Peter D, Weiler C, Izaurralde E
  TITLE     4E-BPs require non-canonical 4E-binding motifs and a lateral surface
            of eIF4E to repress translation.
  JOURNAL   Nat Commun. 2014 Sep 2;5:4790. doi: 10.1038/ncomms5790.
  PUBMED    25179781
REFERENCE   2  (bases 1 to 8564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-09-2"; volume: "5:4790. doi"; pages: " 
            10.1038/ncomms5790"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-Dm4E-T_1-58-DmeIF4Ga_687-16...
FEATURES             Location/Qualifiers
     source          1..8564
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2565
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2585..2650
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2675..2701
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5870..5911
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5921..5938
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5961..5978)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6011..6145
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6035..6054)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6089..6108
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6746..7334)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6826..6845)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7505..8296,8297..8365))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8128..8147
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8366..8470)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8538..8556
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaagaat taattctata ttctaaaaac acaaatgata cttctaaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat aatgaatatg tacacattcg
      241 agaaagaagc gatcaaagaa gcgtcttcgg gcggagtagg agaatgcgga ggagaaggag
      301 aacgagctga tctagtatct ctccacaatc caatgccaac tgaccaactg gccatattcg
      361 gagcaatttg aagccaattt ccatcgcctg gcgatcgctc cattcttggc tatatgtttt
      421 tcaccgttac ccggggccat tttcaaagac tcgtcggcaa gataagattg tgtcactcgc
      481 tgtctctctt catttgtcga agaatgctga ggaatttcgc gatgacgtcg gcgagtattt
      541 tgaagaatga gaataatttg tatttatacg aaaatcagtt agtggaattt tctacaaaaa
      601 catgttatct atagataatt ttgttgcaaa atatgttgac tatgacaaag attgtatgta
      661 tataccttta atgtattctc attttcttat gtatttataa tggcaatgat gatactgatg
      721 atattttaag atgatgccag accaaaaggc ttgaatttct gcgtcttttg ccgaacgcag
      781 tgcatgtgca attgttgttt tttggaatat tcaattttcg gactgtccgc tttgatttca
      841 gtttcttggc ttattcaaaa agcaaagtaa agccaaaaaa gcgagatggc aataccaaat
      901 gcggcaaaac ggtagtggaa ggaaaggggt gcggggcagc ggaaggaagg gtggggcggg
      961 gcgtggcggg gtctgtggct gggcgcgacg tcaccgacgt tggagccact cctttgacca
     1021 tgtgtgcgtg tgtgtattat tcgtgtctcg ccactcgccg gttgtttttt tctttttatg
     1081 ctgcgctctc tctagcgcca tctcgcttac gcatgctcaa cgcaccgcat gttgccgttt
     1141 ccttttatgc gtcattttgg ctcgaaatag gcaattattt aaacaaagat tagtcaacga
     1201 aaacgctaaa ataaataagt ctacaatatg gttacttatt gccatgtgtg tgcagccaac
     1261 gatagcaaca aaagcaacaa cacaggtggc tttccctctt tcactttttg tttgcaagcc
     1321 gcgtgcgagc aagacggcac gaccggcaaa cgcaattacg ctgacaaaga gcagacgaag
     1381 ttttggcgaa aaacatcaag gcgcctgata cgaatgcatt tgcaataaca attgcgatat
     1441 ttaatattgt ttatgaagct gtttgacttc aaaacacaca aaaaaaaaaa taaaacaaat
     1501 tatttgaaag agaattagga atcggacgct tatcgttagg gtaacaacaa gaaatgctta
     1561 ctgagtcaca gcctctggaa aactgccgca agccagagag agagagaaaa agagggagag
     1621 cagcttagac cgcatgtgct tgtgtgtgag gcgtctctct cttcgtctct gttgcgcaaa
     1681 cgcatagact gcactgaaaa aatcgattac ctatttttta tgaatgaata tttgcactat
     1741 tactattcaa aactattaag atagcaatca cattcaatag ccaaatacta taccacctga
     1801 gcgatgcaac gaaatgatca atttgagcaa aaatgctgca tatttaggac ggcatcatta
     1861 tagaaatgct tcttgctgtg tacttttctc tcgtctggca gctgtttcgc cgttattgtt
     1921 aaaaccggct taagttaggt gtgttttcta cgactagtga atgccctact agaagatgtg
     1981 tgttgcacaa aatgtccctg gaataaccaa tttgaagtgc agatagcagt aaacgtaagc
     2041 taatatgaat attatttaac tgtaatgttt taatatcgct ggacattact aataaaccca
     2101 ctataaacac atgtacatat gtatgttttg gcatacaatg agtagttggg gaaaaaatgt
     2161 gtaaaagcac cgtgaccatc acagcataaa gataaccagc tgaagtatcg aatatgagta
     2221 acccccaaat tgaatcacat gccgcaactg ataggaccca tggaagtaca ctcttcatgg
     2281 cgatatacaa gacacacaca agcacgaaca cccagttgcg gaggaaattc tccgtaaatg
     2341 aaaacccaat cggcgaacaa ttcataccca tatatggtaa aagttttgaa cgcgacttga
     2401 gagcggagag cattgcggct gataaggttt tagcgctaag cgggctttat aaaacgggct
     2461 gcgggaccag ttttcatatc actaccgttt gagttcttgt gctgtgtgga tactcctccc
     2521 gacacaaagc cgctccatca gccagcagtc gtctaatcca gagaccccgg atcggggtac
     2581 caacatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag ctcaatggaa
     2641 agctgcaaac ggtggatctc ctccacatat ggcttaccca tacgatgttc cagattacgc
     2701 tcctccaagc ttgaattcta tggatacatc aaagattagt gccaggtact cgaaagtaga
     2761 cctgttagct ctaagatatg aaggtaaaag tcgacagcgc ccgcaatgtt caacacgact
     2821 tgaattgcag acgctaggtt tttggaaaat taatctgaac acagctgcgt taaccgtgtc
     2881 cagcgcctat tctttaatta atatgaaaca gatgtcgatg tctggcgtac aaagtggcgg
     2941 cggtagaagc agtatgaagg gcatgataca cgtaaattta tcattaaatc aagatgtaaa
     3001 gttaagtgaa aatgaaaatg catggcgccc tcgggtctta aataaatctg atggtgatag
     3061 cgacgcgaaa tctgcactcg aaaaagatga attggtacgt cgtgttcggg gtattttaaa
     3121 taagcttacc ccagaaaggt ttgacacact tgttgaggag attataaaat tgaaaattga
     3181 tacacccgac aaagtggacg aagtaattgt gttggtattt gaaaaggcca ttgatgagcc
     3241 aaacttttct gtgtcttacg ccagactatg tcaacgactt gctgcggaag tgaaggtaat
     3301 agacgagcgc atggaaagcg aaaccaaatc taactcggca cactttcgaa atgcattgtt
     3361 agataaaact gaacaggaat tcacccaaaa cgtttcacaa agcaccgcaa aggaaaaaaa
     3421 attacagcca attgtggata aaattaagaa atgtactgat gccaatgaaa aagcggaact
     3481 tgaggcattt ttggaagaag aagaaagaaa aataagaaga cgatccggag ggacagtacg
     3541 atttattggc gaacttttta agatatctat gttgactggc aaaataatat actcttgcat
     3601 cgatacactt cttaaccctc attctgaaga catgcttgag tgtttgtgca agctgcttac
     3661 aactgtgggt gctaaatttg aaaagacccc agtaaattcc aaagacccta gtcggtgtta
     3721 ttcgttggaa aaatcgatca ccaggatgca ggccatcgca tcgaaaactg acaaagatgg
     3781 agccagagta agctcaagag tacgatttat gcttcaagac gtcatcgact tgcgtaagaa
     3841 taaatggcaa acttcgcgga atgaagcacc caaaactatg ggacaaattg aaaaagaggc
     3901 aaagaatgag cagctttctg cacaatattt tggtacactt tccagtacta cacccggagg
     3961 atcgcagggc ggctctggta aacgagatga ccgtggaaac tctcgatacg gtgaatctcg
     4021 ttcaagtagc gcgtatggtg gcagccacag tcaacgtggt gataatggca accttcgaca
     4081 ccagcagcag aacaacgtcg ggggaaacgt ctctggaggt gctggacact ccaatggaaa
     4141 caatgatgaa aatacttggc atgtgcaaac cagcaaaggt agccgctctc tagcggttga
     4201 cagcaataaa ctggaaggcc tgtctaaact ttctgaccaa aatctggaaa caaagaagat
     4261 gggtggattg acgcaattca tttggattag tagtgacacg acaagactat cgtcggcgcc
     4321 gacaccaacc ccatccaatc ctttcgctgt gctctcatcc ctaattgata aaaatagtaa
     4381 tgaaagggat cgcgatcgca gtggacccag aaacaaagga tcatacaaca aaggttctat
     4441 ggagcgggat cgttatgata gaggtatgca ttcgagaact ggatcatctc agggatcacg
     4501 agaaaactca tcctcccgcg gtggtcagca gggtcgaact ttattgagtt catcagttca
     4561 aaaatctact agtcattcaa aatacaccca acaggcacca cctacacggc atactgttaa
     4621 agcccagtca tcagtgggca gctctaacgt taacacaggt ccactctacc gtggaagtga
     4681 gcagcaaaca tcagcaacct tctcgcagac cacccgttct gttgctcctg tagcagtgtt
     4741 catcgaagct agcgaaacag atttaaaact catcaaatct gttgtttcag aaattgtaga
     4801 cctatcagct gcatctaaag aagtcacgcc cggtgcagtt tcgtgcataa aacgagtacc
     4861 agagaaacta cggtgcagtt tcatttatta tattttaaca gattatttgc atttagcaaa
     4921 tgtaggtaaa cagtacaggc ggtatttatc tattgccgtc tcccaactta ttcagcagaa
     4981 ttacatttct gccgatcatt tgagactggc ttacaacgaa tttaccgtat atgcaaacga
     5041 tttgattgtt gatattccgg agctttggct atacattctt caatttgccg ggccactaat
     5101 tgtaaagaaa atattgacta tatcagattt gtggaacaac aatttaaaag aaaatagtcc
     5161 atcaaacgtc gctaaaaagt tccttaaaac atacttgata tattgcacac aagaagttgg
     5221 accaaatttt gctcggaata tgtggatcaa gtttaaccta aaatggtcag attttatgcc
     5281 tgaaagtgaa gttgccgact ttattaaatt taacagactg gaatatgtgg aaaacgaatc
     5341 aaagtcgcca gtgattgatc atagagagac tccagaaaag cacgttaaaa atgtgataga
     5401 ccatattgag cacttactaa aggaaggaac aacggcggac tgcattattg attatagtaa
     5461 cggaaatata atggtggttg ataagttgtt tatcagagga ttgacggaaa cattgagtaa
     5521 tttctcgatt cactacaagg acaacagtta caagcttgag tccgaaacct ttcaaaagtt
     5581 ttgcataccg gttttgcaga gatacattga ttctaatgaa gaccaccaac ttgaatgttt
     5641 atatactttg cagcttttag ttcatggatt ggagcatcca agagggttgc tgagtgaact
     5701 aattggcgag ctatatgatg cctttgtaat acagaaggaa tcgctttgta agtggcgaga
     5761 ttcaaaggat cagtctgctg gcaagggcgt tgctgtaaaa agtcttaatc ctttttttaa
     5821 ctcgttgtta aacgatgatg ccaacagtct agagggcccg cggttcgaag gtaagcctat
     5881 ccctaaccct ctcctcggtc tcgattctac gcgtaccggt catcatcacc atcaccattg
     5941 agtttaaacc cgctgatcag cctcgactgt gccttctaag gcctgagctc gctgatcagc
     6001 ctcgatcgag gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     6061 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     6121 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     6181 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
     6241 tgattatgat cagtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt
     6301 gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg
     6361 taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg
     6421 cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct
     6481 tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg
     6541 cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc
     6601 ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt
     6661 ccaggcaggt agatgacgac catcagggac agcttcaagg ccagcaaaag gccaggaacc
     6721 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     6781 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     6841 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     6901 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     6961 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     7021 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     7081 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     7141 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     7201 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     7261 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     7321 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     7381 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     7441 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     7501 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     7561 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     7621 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     7681 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     7741 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     7801 gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     7861 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     7921 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     7981 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     8041 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     8101 gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag
     8161 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     8221 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     8281 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     8341 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     8401 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     8461 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     8521 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgt
//
