LOCUS       sequence-357279-        4523 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor with homologous arms for Rab11b to knock in DExCon-mNeonGreen 
            module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Gemperle J, Harrison TS, Flett C, Adamson AD, Caswell PT
  TITLE     On demand expression control of endogenous genes with DExCon, 
            DExogron and LUXon reveals differential dynamics of Rab11 family 
            members.
  JOURNAL   Elife. 2022 Jun 16;11. pii: 76651. doi: 10.7554/eLife.76651.
  PUBMED    35708998
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-06-16"; volume: "11. pii: 76651. doi"; pages: " 
            10.7554/eLife.76651"
COMMENT     Sequence Label: pJET-67-DExCon-mNeonGreen-R11b
FEATURES             Location/Qualifiers
     source          1..4523
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    11..29
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    47..65
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    83..101
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    119..137
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    155..173
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    191..209
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    227..245
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(558..576)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(626..644)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(950..968)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1036..1140
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1141..1209,1210..2001)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1359..1378)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2172..2760
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2661..2680
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2914..2931
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3051..3072
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3087..3092,3093..3110,3111..3117)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     regulatory      4122..4131
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcttatacgt tctctatcac tgatagggag taaactggat atacgttctc tatcactgat
       61 agggagtaaa ctgtagatac gttctctatc actgataggg agtaaactgg tcatacgttc
      121 tctatcactg atagggagta aactccttat acgttctcta tcactgatag ggagtaaagt
      181 ctgcatacgt tctctatcac tgatagggag taaactcttc atacgttctc tatcactgat
      241 agggagtaaa gaatttgtcc tggcgcttcc ggcgggatcg gcgactccgc agccccacca
      301 caaacacccg acgggggcgg agccggcgcc gcgcagagcg gcggttgaat ggcctatcag
      361 cggcgagtgg agtagcgacg ggcacccagc gaagccaatc agagatggaa gtagtgctct
      421 gagggtgggc gccgcttggt accaccctcc tcgccctcgg tgtcctggag aaaggcggaa
      481 ggaatgcgga cctttttgaa gtgacggacg cgccagccta tcaggggcga gctcaagagg
      541 gcggggcgga agactgccct atagtgagtc gtattatatc ttgctgaaaa actcgagcca
      601 tccggaagat ctggcggccg ctctccctat agtgagtcgt attacgccgg atggatatgg
      661 tgttcaggca caagtgttaa agcagttgat tttattcact atgatgaaaa aaacaatgaa
      721 tggaacctgc tccaagttaa aaatagagat aataccgaaa actcatcgag tagtaagatt
      781 agagataata caacaataaa aaaatggttt agaacttact cacagcgtga tgctactaat
      841 tgggacaatt ttccagatga agtatcatct aagaatttaa atgaagaaga cttcagagct
      901 tttgttaaaa attatttggc aaaaataata taattcggct gcaggggcgg cctcgtgata
      961 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     1021 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     1081 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     1141 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     1201 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     1261 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     1321 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     1381 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     1441 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     1501 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     1561 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     1621 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     1681 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     1741 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     1801 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     1861 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     1921 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     1981 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     2041 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     2101 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     2161 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2221 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2281 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     2341 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2401 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2461 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     2521 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     2581 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     2641 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     2701 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     2761 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     2821 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     2881 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     2941 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     3001 cacgacaggt ttcccgactg gaaagcaatt ggcagtgagc gcaacgcaat taatgtgagt
     3061 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt
     3121 ggaattatga gcggataata atttcacaca ggaggtttaa actttaaaca tgtcaaaaga
     3181 gacgtctttt gttaagaatg ctgaggaact tgcaaagcaa aaaatggatg ctattaaccc
     3241 tgaactttct tcaaaattta aatttttaat aaaattcctg tctcagtttc ctgaagcttg
     3301 ctctaaacct cgttcaaaaa aaatgcagaa taaagttggt caagaggaac atattgaata
     3361 tttagctcgt agttttcatg agagtcgatt gccaagaaaa cccacgccac ctacaacggt
     3421 tcctgatgag gtggttagca tagttcttaa tataagtttt aatatacagc ctgaaaatct
     3481 tgagagaata aaagaagaac atcgattttc catggcagct gagaatattg taggagatct
     3541 tctagaaaga tagcgcggcc ttggcccgtc tgcccgccgc ctcgccgcca cgacttcgcc
     3601 cgtctgtgcc ccaccggccg cacctttgaa taggtagtcg tactcgtcgt cccgggtacc
     3661 acttccagca ccggttccct tgtacagctc gtccatgccc atcacgtcgg tgaaggcttt
     3721 ctgccactct ttgaagttca gttctgtctt ggagtgcttc agctcggtct tgcggaacac
     3781 gtacataggc tggttcttca ggtagttggc ggccataggt ttggcgaagg tgtaggtggt
     3841 tctggcggtg cttctgtacc gcttgccgtt gccggtggtg taggaccact tgaaggtgct
     3901 gatgatggtc ttgtcgttgg ggtatgtttt cttgcttctg caccagtcgg cggctgtcag
     3961 agaattggtc atcacggggc catcagcagg gaagcctgtg cctttcactt gggcctcgcc
     4021 tttgatgtgg ctgccctcgt atgtgtatct gtagttcacg gtcaggctgg cgccatcttc
     4081 aaactgcatg gtccggtgca cctggtagcc agagccatca accatggcgg cctggaaagg
     4141 gctcatgccg tcaggataag gcaggtactg gtggaagccg tagccgatgt gaggcaccag
     4201 aatccagggg ctgaactgca ggtcgccctt ggtggacttc aggttcagtt cctcgtagcc
     4261 gtcgttggga ttgccggtgc cttgtccgac catgtcgaag tccacgccgt tgatgctgcc
     4321 gaagatgtgc agctcgtgtg tggcaggcag gctggccata ttgtcctctt cgcccttaga
     4381 cacgccacta gtcccacccg atcccatctt tacgagggta ggaagtggta cggaaagttg
     4441 gtataagaca aaagtgttgt ggaattgaag tttactcaaa aaatcagcac tcttttatag
     4501 gcgccctggt ttacataagc aaa
//
