LOCUS       sequence-357409-       15826 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  to delete miR200 family genes including miR-141, miR-200c, miR-429 
            and miR-200b .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15826)
  AUTHORS   Yu L, Cao C, Li X, Zhang M, Gu Q, Gao H, Balic JJ, Xu D, Zhang L, 
            Ying L, Xu D, Yang Y, Wu D, He B, Jenkins BJ, Liu Y, Li J
  TITLE     Complete loss of miR-200 family induces EMT associated cellular 
            senescence in gastric cancer.
  JOURNAL   Oncogene. 2021 Oct 19. pii: 10.1038/s41388-021-02067-y. doi: 
            10.1038/s41388-021-02067-y.
  PUBMED    34667277
REFERENCE   2  (bases 1 to 15826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene. 
            2021 Oct 19. pii: 10.1038/s41388-021-02067-y. doi: 
            10.1038/s41388-021-02067-y."
COMMENT     Sequence Label: Multiplex-CRISPR-miR-200FAM-T2A-GFP
FEATURES             Location/Qualifiers
     source          1..15826
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(80..328)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(138..157)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(308..328)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        358..572
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     493..512
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_RNA        602..677
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        complement(707..782)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        1079..2290
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2127..2146
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      2319..2328
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2328..2393
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2400..2420
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2430..6533
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2430..6533
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2430..6533
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6546..6566
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6573..6626
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(6633..6635,6636..7346)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(6633..6635,6636..7346)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(6675..6696)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6936..6955)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7283..7304
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(7362..7395)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    7420..8008
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7473..7493)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7891..7902)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8011..8027)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8012..8028)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8533..8713
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8739..8756)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8745..8969
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9015..9443
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9102..9121)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9312..9333
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9452..9472)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9457..9786
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9637..9772
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9699..9718
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9834..9881
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9900..10274
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10404..10525
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10441..10460)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10495..10514
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10574..10590)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10574..10590)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10587..10609)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10598..10614
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10622..10628,10629..10646,10647..10652))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10667..10688
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10805..10822)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10976..11564)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11056..11075)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11735..12526,12527..12595))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12358..12377
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12596..12700)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12775..12794)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        12966..13345
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        13347..13545
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     13500..13520
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             13563..13743
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    13790..13915
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    14408..14641
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             14826..14870
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    15168..15285
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(15330..15363)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        15368..15681
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     15600..15620
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     misc_RNA        15704..15779
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        complement(join(15809..15826,1..58))
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tttttcaagt tgataacgga ctagccttat tttaacttgc tatttctagc tctaaaacga
       61 cagtattaga cagagggccg gtgtttcgtc ctttccacaa gatatataaa gccaagaaat
      121 cgaaatactt tcaagttacg gtaagcatat gatagtccat tttaaaacat aattttaaaa
      181 ctgcaaacta cccaagaaat tattactttc tacgtcacgt attttgtact aatatctttg
      241 tgtttacagt caaattaatt ccaattatct ctctaacagc cttgtatcgt atatgcaaat
      301 atgaaggaat catgggaaat aggccctctt cctgcccgac cttggatcga tccgcctgaa
      361 cgctgacgtc atcaacccgc tccaaggaat cgcgggccca gtgtcactag gcgggaacac
      421 ccagcgcgcg tgcgccctgg caggaagatg gctgtgaggg acaggggagt ggcgccctgc
      481 aatatttgca tgtcgctatg tgttctggga aatcaccata aacgtgaaat gtctttggat
      541 ttgggaatct tataagttct gtatgagacc actctttccc atccaacact gtactggaag
      601 agttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag
      661 tggcaccgag tcggtgcttt ttttggtcat cagtccgaga aaaaaagcac cgactcggtg
      721 ccactttttc aagttgataa cggactagcc ttattttaac ttgctatttc tagctctaaa
      781 acaacactgc tgggtaagac gacgaggtac ccaagcggcg cacaagctat ataaacctga
      841 aggaagtctc aactttacac ttaggtcaag ttgcttatcg tactagagct tcagcaggaa
      901 atttaactaa aatctaattt aaccagcata gcaaatatca tttattccca aaatgctaaa
      961 gtttgagata aacggacttg atttccggct gttttgacac tatccagaat gccttgcaga
     1021 tgggtggggc atgctaaata ctgcagacaa ttaattaacc cgtgtcggct ccagatctgg
     1081 cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc tcctcacggc gagcgctgcc
     1141 acgtcagacg aagggcgcag cgagcgtcct gatccttccg cccggacgct caggacagcg
     1201 gcccgctgct cataagactc ggccttagaa ccccagtatc agcagaagga cattttagga
     1261 cgggacttgg gtgactctag ggcactggtt ttctttccag agagcggaac aggcgaggaa
     1321 aagtagtccc ttctcggcga ttctgcggag ggatctccgt ggggcggtga acgccgatga
     1381 ttatataagg acgcgccggg tgtggcacag ctagttccgt cgcagccggg atttgggtcg
     1441 cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct gctgggctgg
     1501 ccggggcttt cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga gagaccgcca
     1561 agggctgtag tctgggtccg cgagcaaggt tgccctgaac tgggggttgg ggggagcgca
     1621 gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc ttgtgaggcg ggctgtgagg
     1681 tcgttgaaac aaggtggggg gcatggtggg cggcaagaac ccaaggtctt gaggccttcg
     1741 ctaatgcggg aaagctctta ttcgggtgag atgggctggg gcaccatctg gggaccctga
     1801 cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc tgttgcgggg gcggcagtta
     1861 tggcggtgcc gttgggcagt gcacccgtac ctttgggagc gcgcgccctc gtcgtgtcgt
     1921 gacgtcaccc gttctgttgg cttataatgc agggtggggc cacctgccgg taggtgtgcg
     1981 gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta gggtaggctc tcctgaatcg
     2041 acaggcgccg gacctctggt gaggggaggg ataagtgagg cgtcagtttc tttggtcggt
     2101 tttatgtacc tatcttctta agtagctgaa gctccggttt tgaactatgc gctcggggtt
     2161 ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa atgtaatcat ttgggtcaat
     2221 atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa ttctggccgt ttttggcttt
     2281 tttgttagac gaagcttggg ctgcaggtcg actctagagc caccatggac tacaaagacc
     2341 atgacggtga ttataaagat catgacatcg attacaagga tgacgatgac aagatggccc
     2401 ccaagaagaa gaggaaggtg ggccgcggaa tggacaagaa gtactccatt gggctcgata
     2461 tcggcacaaa cagcgtcggc tgggccgtca ttacggacga gtacaaggtg ccgagcaaaa
     2521 aattcaaagt tctgggcaat accgatcgcc acagcataaa gaagaacctc attggcgccc
     2581 tcctgttcga ctccggggaa accgccgaag ccacgcggct caaaagaaca gcacggcgca
     2641 gatatacccg cagaaagaat cggatctgct acctgcagga gatctttagt aatgagatgg
     2701 ctaaggtgga tgactctttc ttccataggc tggaggagtc ctttttggtg gaggaggata
     2761 aaaagcacga gcgccaccca atctttggca atatcgtgga cgaggtggcg taccatgaaa
     2821 agtacccaac catatatcat ctgaggaaga agcttgtaga cagtactgat aaggctgact
     2881 tgcggttgat ctatctcgcg ctggcgcata tgatcaaatt tcggggacac ttcctcatcg
     2941 agggggacct gaacccagac aacagcgatg tcgacaaact ctttatccaa ctggttcaga
     3001 cttacaatca gcttttcgaa gagaacccga tcaacgcatc cggagttgac gccaaagcaa
     3061 tcctgagcgc taggctgtcc aaatcccggc ggctcgaaaa cctcatcgca cagctccctg
     3121 gggagaagaa gaacggcctg tttggtaatc ttatcgccct gtcactcggg ctgaccccca
     3181 actttaaatc taacttcgac ctggccgaag atgccaagct tcaactgagc aaagacacct
     3241 acgatgatga tctcgacaat ctgctggccc agatcggcga ccagtacgca gacctttttt
     3301 tggcggcaaa gaacctgtca gacgccattc tgctgagtga tattctgcga gtgaacacgg
     3361 agatcaccaa agctccgctg agcgctagta tgatcaagcg ctatgatgag caccaccaag
     3421 acttgacttt gctgaaggcc cttgtcagac agcaactgcc tgagaagtac aaggaaattt
     3481 tcttcgatca gtctaaaaat ggctacgccg gatacattga cggcggagca agccaggagg
     3541 aattttacaa atttattaag cccatcttgg aaaaaatgga cggcaccgag gagctgctgg
     3601 taaagcttaa cagagaagat ctgttgcgca aacagcgcac tttcgacaat ggaagcatcc
     3661 cccaccagat tcacctgggc gaactgcacg ctatcctcag gcggcaagag gatttctacc
     3721 cctttttgaa agataacagg gaaaagattg agaaaatcct cacatttcgg ataccctact
     3781 atgtaggccc cctcgcccgg ggaaattcca gattcgcgtg gatgactcgc aaatcagaag
     3841 agaccatcac tccctggaac ttcgaggaag tcgtggataa gggggcctct gcccagtcct
     3901 tcatcgaaag gatgactaac tttgataaaa atctgcctaa cgaaaaggtg cttcctaaac
     3961 actctctgct gtacgagtac ttcacagttt ataacgagct caccaaggtc aaatacgtca
     4021 cagaagggat gagaaagcca gcattcctgt ctggagagca gaagaaagct atcgtggacc
     4081 tcctcttcaa gacgaaccgg aaagttaccg tgaaacagct caaagaagac tatttcaaaa
     4141 agattgaatg tttcgactct gttgaaatca gcggagtgga ggatcgcttc aacgcatccc
     4201 tgggaacgta tcacgatctc ctgaaaatca ttaaagacaa ggacttcctg gacaatgagg
     4261 agaacgagga cattcttgag gacattgtcc tcacccttac gttgtttgaa gatagggaga
     4321 tgattgaaga acgcttgaaa acttacgctc atctcttcga cgacaaagtc atgaaacagc
     4381 tcaagaggcg ccgatataca ggatgggggc ggctgtcaag aaaactgatc aatgggatcc
     4441 gagacaagca gagtggaaag acaatcctgg attttcttaa gtccgatgga tttgccaacc
     4501 ggaacttcat gcagttgatc catgatgact ctctcacctt taaggaggac atccagaaag
     4561 cacaagtttc tggccagggg gacagtcttc acgagcacat cgctaatctt gcaggtagcc
     4621 cagctatcaa aaagggaata ctgcagaccg ttaaggtcgt ggatgaactc gtcaaagtaa
     4681 tgggaaggca taagcccgag aatatcgtta tcgagatggc ccgagagaac caaactaccc
     4741 agaagggaca gaagaacagt agggaaagga tgaagaggat tgaagagggt ataaaagaac
     4801 tggggtccca aatccttaag gaacacccag ttgaaaacac ccagcttcag aatgagaagc
     4861 tctacctgta ctacctgcag aacggcaggg acatgtacgt ggatcaggaa ctggacatca
     4921 atcggctctc cgactacgac gtggatcata tcgtgcccca gtcttttctc aaagatgatt
     4981 ctattgataa taaagtgttg acaagatccg ataaaaatag agggaagagt gataacgtcc
     5041 cctcagaaga agttgtcaag aaaatgaaaa attattggcg gcagctgctg aacgccaaac
     5101 tgatcacaca acggaagttc gataatctga ctaaggctga acgaggtggc ctgtctgagt
     5161 tggataaagc cggcttcatc aaaaggcagc ttgttgagac acgccagatc accaagcacg
     5221 tggcccaaat tctcgattca cgcatgaaca ccaagtacga tgaaaatgac aaactgattc
     5281 gagaggtgaa agttattact ctgaagtcta agctggtctc agatttcaga aaggactttc
     5341 agttttataa ggtgagagag atcaacaatt accaccatgc gcatgatgcc tacctgaatg
     5401 cagtggtagg cactgcactt atcaaaaaat atcccaagct tgaatctgaa tttgtttacg
     5461 gagactataa agtgtacgat gttaggaaaa tgatcgcaaa gtctgagcag gaaataggca
     5521 aggccaccgc taagtacttc ttttacagca atattatgaa ttttttcaag accgagatta
     5581 cactggccaa tggagagatt cggaagcgac cacttatcga aacaaacgga gaaacaggag
     5641 aaatcgtgtg ggacaagggt agggatttcg cgacagtccg gaaggtcctg tccatgccgc
     5701 aggtgaacat cgttaaaaag accgaagtac agaccggagg cttctccaag gaaagtatcc
     5761 tcccgaaaag gaacagcgac aagctgatcg cacgcaaaaa agattgggac cccaagaaat
     5821 acggcggatt cgattctcct acagtcgctt acagtgtact ggttgtggcc aaagtggaga
     5881 aagggaagtc taaaaaactc aaaagcgtca aggaactgct gggcatcaca atcatggagc
     5941 gatcaagctt cgaaaaaaac cccatcgact ttctcgaggc gaaaggatat aaagaggtca
     6001 aaaaagacct catcattaag cttcccaagt actctctctt tgagcttgaa aacggccgga
     6061 aacgaatgct cgctagtgcg ggcgagctgc agaaaggtaa cgagctggca ctgccctcta
     6121 aatacgttaa tttcttgtat ctggccagcc actatgaaaa gctcaaaggg tctcccgaag
     6181 ataatgagca gaagcagctg ttcgtggaac aacacaaaca ctaccttgat gagatcatcg
     6241 agcaaataag cgaattctcc aaaagagtga tcctcgccga cgctaacctc gataaggtgc
     6301 tttctgctta caataagcac agggataagc ccatcaggga gcaggcagaa aacattatcc
     6361 acttgtttac tctgaccaac ttgggcgcgc ctgcagcctt caagtacttc gacaccacca
     6421 tagacagaaa gcggtacacc tctacaaagg aggtcctgga cgccacactg attcatcagt
     6481 caattacggg gctctatgaa acaagaatcg acctctctca gctcggtgga gacagcaggg
     6541 ctgaccccaa gaagaagagg aaggtggcta gcgagggcag aggaagtctt ctaacatgcg
     6601 gtgacgtgga ggagaatccc ggccctggta ccgtgagcaa gggcgaggag ctgttcaccg
     6661 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     6721 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     6781 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     6841 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     6901 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     6961 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     7021 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     7081 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     7141 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     7201 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     7261 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     7321 ctctcggcat ggacgagctg tacaagaccg gttgataata gataacttcg tatagcatac
     7381 attatacgaa gttatgaatt cgatatcaag cttatcgata atcaacctct ggattacaaa
     7441 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     7501 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     7561 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     7621 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     7681 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     7741 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     7801 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     7861 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     7921 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     7981 cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgaga cctagaaaaa
     8041 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
     8101 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg
     8161 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     8221 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     8281 tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt
     8341 ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga
     8401 gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     8461 gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat
     8521 ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     8581 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     8641 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     8701 aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     8761 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     8821 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     8881 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     8941 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     9001 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     9061 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     9121 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     9181 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     9241 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     9301 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     9361 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     9421 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     9481 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     9541 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     9601 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     9661 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     9721 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     9781 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca
     9841 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca
     9901 tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg
     9961 agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg
    10021 tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca
    10081 acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg
    10141 tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc
    10201 cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg
    10261 aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt
    10321 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
    10381 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
    10441 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    10501 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
    10561 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
    10621 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
    10681 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
    10741 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
    10801 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
    10861 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
    10921 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
    10981 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
    11041 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
    11101 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
    11161 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
    11221 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
    11281 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
    11341 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
    11401 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
    11461 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
    11521 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
    11581 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
    11641 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
    11701 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
    11761 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
    11821 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
    11881 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
    11941 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
    12001 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
    12061 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
    12121 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
    12181 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    12241 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    12301 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    12361 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    12421 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    12481 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    12541 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    12601 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    12661 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    12721 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat
    12781 ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc
    12841 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat
    12901 gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac
    12961 gcgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
    13021 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
    13081 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
    13141 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
    13201 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
    13261 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
    13321 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
    13381 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
    13441 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
    13501 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta
    13561 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
    13621 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
    13681 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
    13741 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
    13801 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
    13861 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
    13921 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
    13981 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
    14041 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
    14101 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
    14161 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
    14221 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
    14281 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
    14341 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
    14401 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
    14461 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
    14521 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
    14581 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
    14641 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
    14701 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
    14761 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
    14821 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
    14881 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
    14941 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
    15001 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
    15061 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg
    15121 cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa
    15181 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
    15241 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
    15301 gacagcagag atccagtttg gttaattaaa taacttcgta tagcatacat tatacgaagt
    15361 tatgatagat ccgacgccgc catctctagg cccgcgccgg ccccctcgca cagacttgtg
    15421 ggagaagctc ggctactccc ctgccccggt taatttgcat ataatatttc ctagtaacta
    15481 tagaggctta atgtgcgata aaagacagat aatctgttct ttttaatact agctacattt
    15541 tacatgatag gcttggattt ctataagaga tacaaatact aaattattat tttaaaaaac
    15601 agcacaaaag gaaactcacc ctaactgtaa agtaattgtg tgttttgaga ctataaatat
    15661 cccttggaga aaagccttgt ttggccatct tactgggcag catgttttag agctagaaat
    15721 agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct
    15781 ttttttgtac gctcagcgga gaaaaaaagc accgactcgg tgccac
//
