LOCUS       sequence-357475-       20413 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pCCI vector encoding YFV-17D lacking nonstructural protein 1 (NS1) 
            but with mVenus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20413)
  AUTHORS   Li E, Guo J, Oh SJ, Luo Y, Oliveros HC, Du W, Arano R, Kim Y, Chen 
            YT, Eitson J, Lin DT, Li Y, Roberts T, Schoggins JW, Xu W
  TITLE     Anterograde transneuronal tracing and genetic control with 
            engineered yellow fever vaccine YFV-17D.
  JOURNAL   Nat Methods. 2021 Nov 25. pii: 10.1038/s41592-021-01319-9. doi: 
            10.1038/s41592-021-01319-9.
  PUBMED    34824475
REFERENCE   2  (bases 1 to 20413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2021 Nov 25. pii: 10.1038/s41592-021-01319-9. doi: 
            10.1038/s41592-021-01319-9."
COMMENT     Sequence Label: YFVdelNS1-mVenus
FEATURES             Location/Qualifiers
     source          1..20413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(227..1198)
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(1198..2373)
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    2699..2949
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(2952..3707)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      3798..4017
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      4085..4616
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      4093..4707
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     promoter        5565..5667
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             5668..6327
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(5734..5753)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     protein_bind    6547..6568
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6583..6588,6589..6606,6607..6613)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6621..6637
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6626..6648
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6645..6661
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6645..6661
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        7098..7116
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     7098..7115
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             join(7315..7317,7318..7320,7321..8031)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7360..7381)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7621..7640)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7968..7989
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     18665..18684
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(19048..19067)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(19049..19067)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(19074..19091)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(19074..19090)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(19083..19105)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(19298..19317)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    19385..19418
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    19436..19834
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     misc_feature    join(20094..20413,1..154)
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
ORIGIN
        1 tggtcccaga ctaataatca gaccgacgat acgagtggga ccgtggtccc agactaataa
       61 tcagaccgac gatacgagtg ggaccgtggt cccagtctga ttatcagacc gacgatacaa
      121 gtggaacagt gggcccagag agaatattca ggccagttat gctttctggc ctgtaacaaa
      181 ggacattaag taaagacaga taaacgtaga ctaaaacgtg gtcgcatcag ggtgctggct
      241 tttcaagttc cttaagaatg gcctcaattt tctctataca ctcagttgga acacgagacc
      301 tgtccaggtt aagcaccatt ttatcgccct tatacaatac tgtcgctcca ggagcaaact
      361 gatgtcgtga gcttaaacta gttcttgatg cagatgacgt tttaagcaca gaagttaaaa
      421 gagtgataac ttcttcagct tcaaatatca ccccagcttt tttctgctca tgaaggttag
      481 atgcctgctg cttaagtaat tcctctttat ctgtaaaggc tttttgaagt gcatcacctg
      541 accgggcaga tagttcaccg gggtgagaaa aaagagcaac aactgattta ggcaatttgg
      601 cggtgttgat acagcgggta ataatcttac gtgaaatatt ttccgcatca gccagcgcag
      661 aaatatttcc agcaaattca ttctgcaatc ggcttgcata acgctgacca cgttcataag
      721 cacttgttgg gcgataatcg ttacccaatc tggataatgc agccatctgc tcatcatcca
      781 gctcgccaac cagaacacga taatcacttt cggtaagtgc agcagcttta cgacggcgac
      841 tcccatcggc aatttctatg acaccagata ctcttcgacc gaacgccggt gtctgttgac
      901 cagtcagtag aaaagaaggg atgagatcat ccagtgcgtc ctcagtaagc agctcctggt
      961 cacgttcatt acctgaccat acccgagagg tcttctcaac actatcaccc cggagcactt
     1021 caagagtaaa cttcacatcc cgaccacata caggcaaagt aatggcatta ccgcgagcca
     1081 ttactcctac gcgcgcaatt aacgaatcca ccatcggggc agctggtgtc gataacgaag
     1141 tatcttcaac cggttgagta ttgagcgtat gttttggaat aacaggcgca cgcttcatta
     1201 tctaatctcc cagcgtggtt taatcagacg atcgaaaatt tcattgcaga caggttccca
     1261 aatagaaaga gcatttctcc aggcaccagt tgaagagcgt tgatcaatgg cctgttcaaa
     1321 aacagttctc atccggatct gacctttacc aacttcatcc gtttcacgta caacattttt
     1381 tagaaccatg cttccccagg catcccgaat ttgctcctcc atccacgggg actgagagcc
     1441 attactattg ctgtatttgg taagcaaaat acgtacatca ggctcgaacc ctttaagatc
     1501 aacgttcttg agcagatcac gaagcatatc gaaaaactgc agtgcggagg tgtagtcaaa
     1561 caactcagca ggcgtgggaa caatcagcac atcagcagca catacgacat taatcgtgcc
     1621 gatacccagg ttaggcgcgc tgtcaataac tatgacatca tagtcatgag caacagtttc
     1681 aatggccagt cggagcatca ggtgtggatc ggtgggcagt ttaccttcat caaatttgcc
     1741 cattaactca gtttcaatac ggtgcagagc cagacaggaa ggaataatgt caagccccgg
     1801 ccagcaagtg ggctttattg cataagtgac atcgtccttt tccccaagat agaaaggcag
     1861 gagagtgtct tctgcatgaa tatgaagatc tggtacccat ccgtgataca ttgaggctgt
     1921 tccctggggg tcgttacctt ccacgagcaa aacacgtagc cccttcagag ccagatcctg
     1981 agcaagatga acagaaactg aggttttgta aacgccacct ttatgggcag caaccccgat
     2041 caccggtgga aatacgtctt cagcacgtcg caatcgcgta ccaaacacat cacgcatatg
     2101 attaatttgt tcaattgtat aaccaacacg ttgctcaacc cgtcctcgaa tttccatatc
     2161 cgggtgcggt agtcgccctg ctttctcggc atctctgata gcctgagaag aaaccccaac
     2221 taaatccgct gcttcaccta ttctccagcg ccgggttatt ttcctcgctt ccgggctgtc
     2281 atcattaaac tgtgcaatgg cgatagcctt cgtcatttca tgaccagcgt ttatgcactg
     2341 gttaagtgtt tccatgagtt tcattctgaa catcctttaa tcattgcttt gcgttttttt
     2401 attaaatctt gcaatttact gcaaagcaac aacaaaatcg caaagtcatc aaaaaaccgc
     2461 aaagttgttt aaaataagag caacactaca aaaggagata agaagagcac atacctcagt
     2521 cacttattat cactagcgct cgccgcagcc gtgtaaccga gcatagcgag cgaactggcg
     2581 aggaagcaaa gaagaactgt tctgtcagat agctcttacg ctcagcgcaa gaagaaatat
     2641 ccaccgtggg aaaaactcca ggtagaggta cacacgcgga tagccaattc agagtaataa
     2701 actgtgataa tcaaccctca tcaatgatga cgaactaacc cccgatatca agtcacatga
     2761 cgaagggaaa gagaaggaaa tcaactgtga caaactgccc tcaaatttgg cttccttaaa
     2821 aattacagtt caaaaagtat gagaaaatcc atgcaggctg aaggaaacag caaaactgtg
     2881 acaaattacc ctcagtaggt cagaacaaat gtgacgaacc accctcaaat ctgtgacaga
     2941 taaccctcag actatcctgt cgtcatggaa gtgatatcgc ggaaggaaaa tacgatatga
     3001 gtcgtctggc ggcctttctt tttctcaatg tatgagaggc gcattggagt tctgctgttg
     3061 atctcattaa cacagacctg caggaagcgg cggcggaagt caggcatacg ctggtaactt
     3121 tgaggcagct ggtaacgctc tatgatccag tcgattttca gagagacgat gcctgagcca
     3181 tccggcttac gatactgaca cagggattcg tataaacgca tggcatacgg attggtgatt
     3241 tcttttgttt cactaagccg aaactgcgta aaccggttct gtaacccgat aaagaaggga
     3301 atgagatatg ggttgatatg tacactgtaa agccctctgg atggactgtg cgcacgtttg
     3361 ataaaccaag gaaaagattc atagcctttt tcatcgccgg catcctcttc agggcgataa
     3421 aaaaccactt ccttccccgc gaaactcttc aatgcctgcc gtatatcctt actggcttcc
     3481 gcagaggtca atccgaatat ttcagcatat ttagcaacat ggatctcgca gataccgtca
     3541 tgttcctgta gggtgccatc agattttctg atctggtcaa cgaacagata cagcatacgt
     3601 ttttgatccc gggagagact atatgccgcc tcagtgaggt cgtttgactg gacgattcgc
     3661 gggctatttt tacgtttctt gtgattgata accgctgttt ccgccatgac agatccatgt
     3721 gaagtgtgac aagtttttag attgtcacac taaataaaaa agagtcaata agcagggata
     3781 actttgtgaa aaaacagctt cttctgaggg caatttgtca cagggttaag ggcaatttgt
     3841 cacagacagg actgtcattt gagggtgatt tgtcacactg aaagggcaat ttgtcacaac
     3901 accttctcta gaaccagcat ggataaaggc ctacaaggcg ctctaaaaaa gaagatctaa
     3961 aaactataaa aaaaataatt ataaaaatat ccccgtggat aagtggataa ccccaaggga
     4021 agttttttca ggcatcgtgt gtaagcagaa tatataagtg ctgttccctg gtgcttcctc
     4081 gctcactcga ccgggagggt tcgagaaggg ggggcacccc ccttcggcgt gcgcggtcac
     4141 gcgcacaggg cgcagccctg gttaaaaaca aggtttataa atattggttt aaaagcaggt
     4201 taaaagacag gttagcggtg gccgaaaaac gggcggaaac ccttgcaaat gctggatttt
     4261 ctgcctgtgg acagcccctc aaatgtcaat aggtgcgccc ctcatctgtc agcactctgc
     4321 ccctcaagtg tcaaggatcg cgcccctcat ctgtcagtag tcgcgcccct caagtgtcaa
     4381 taccgcaggg cacttatccc caggcttgtc cacatcatct gtgggaaact cgcgtaaaat
     4441 caggcgtttt cgccgatttg cgaggctggc cagctccacg tcgccggccg aaatcgagcc
     4501 tgcccctcat ctgtcaacgc cgcgccgggt gagtcggccc ctcaagtgtc aacgtccgcc
     4561 cctcatctgt cagtgagggc caagttttcc gcgaggtatc cacaacgccg gcggccggcc
     4621 gcggtgtctc gcacacggct tcgacggcgt ttctggcgcg tttgcagggc catagacggc
     4681 cgccagccca gcggcgaggg caaccagccg agggcttcgc cctgtcgctc gactgcggcg
     4741 agcactactg gctgtaaaag gacagaccac atcatggttc tgtgttcatt aggttgttct
     4801 gtccattgct gacataatcc gctccacttc aacgtaacac cgcacgaaga tttctattgt
     4861 tcctgaaggc atattcaaat cgttttcgtt accgcttgca ggcatcatga cagaacacta
     4921 cttcctataa acgctacaca ggctcctgag attaataatg cggatctcta cgataatggg
     4981 agattttccc gactgtttcg ttcgcttctc agtggataac agccagcttc tctgtttaac
     5041 agacaaaaac agcatatcca ctcagttcca catttccata taaaggccaa ggcatttatt
     5101 ctcaggataa ttgtttcagc atcgcaaccg catcagactc cggcatcgca aactgcaccc
     5161 ggtgccgggc agccacatcc agcgcaaaaa ccttcgtgta gacttccgtt gaactgatgg
     5221 acttatgtcc catcaggctt tgcagaactt tcagcggtat accggcatac agcatgtgca
     5281 tcgcatagga atggcggaac gtatgtggtg tgaccggaac agagaacgtc acaccgtcag
     5341 cagcagcggc ggcaaccgcc tccccaatcc aggtcctgac cgttctgtcc gtcacttccc
     5401 agatccgcgc tttctctgtc cttcctgtgc gacggttacg ccgctccatg agcttatcgc
     5461 gaataaatac ctgtgacgga agatcacttc gcagaataaa taaatcctgg tgtccctgtt
     5521 gataccggga agccctgggc caacttttgg cgaaaatgag acgttgatcg gcacgtaaga
     5581 ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg
     5641 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
     5701 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
     5761 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
     5821 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
     5881 gaatttcgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
     5941 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
     6001 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
     6061 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
     6121 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     6181 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     6241 catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     6301 tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca gttattggtg cccttaaacg
     6361 cctggttgct acgcctgaat aagtgataat aagcggatga atggcagaaa ttcgatgata
     6421 agctgtcaaa catgagaatt ggtcgacggc ccgggcggcc gcaaggagtt cgcgttggcc
     6481 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     6541 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     6601 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     6661 ccatgattac gccaagctat ttaggtgaga ctatagaata ctcaagcttg catgcctgca
     6721 ggtcgactct agaggatccc acattattga agcatttatc agggttattg tctcatgagc
     6781 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     6841 cgaaaagtgc cacctgacgt gtcgacgcgg ccgctagcga tgaccctgct gattggttcg
     6901 ctgaccattt ccgggtgcgg gacggcgtta ccagaaactc agaaggttcg tccaaccaaa
     6961 ccgactctga cggcagttta cgagagagat gatagggtct gcttcagtaa gccagatgct
     7021 acacaattag gcttgtacat attgtcgtta gaacgcggct acaattaata cataacctta
     7081 tgtatcatac acatacgatt taggtgacac tatagagtaa atcctgtgtg ctaattgagg
     7141 tgcattggtc tgcaaatcga gttgctaggc aataaacaca tttggattaa ttttaatcgt
     7201 tcgttgagcg attagcagag aactgaccag aacatgtctg gtcgtaaagc tcagggaaaa
     7261 accctgggcg tcaatatggt acgacgagga gttcgctcct tgtcaaacga gctcatggtg
     7321 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     7381 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     7441 ctgaccctga agctgatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     7501 accaccctgg gctacggcct gcagtgcttc gcccgctacc ccgaccacat gaagcagcac
     7561 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     7621 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     7681 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     7741 gagtacaact acaacagcca caacgtctat atcaccgccg acaagcagaa gaacggcatc
     7801 aaggccaact tcaagatccg ccacaacatc gaggacggcg gcgtgcagct cgccgaccac
     7861 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     7921 agctaccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     7981 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gaccggtaac
     8041 tttgaccttc tcaagttggc cggcgacgtc gagtccaacc cagggcccct gcagcaaatt
     8101 ttcgtgaaga ccctgacggg caagaccatc actcttgagg tcgagcccag tgacaccatc
     8161 gagaatgtca aggccaagat ccaagacaag gaaggcatcc cacctgacca gcagaggctg
     8221 atattcgcgg gcaaacagct ggaggatggc cgcaccctgt ccgactacaa catccagaaa
     8281 gagtccacct tgcacctggt gctgtgtctc cgcggtggaa tgtccggtcg taaagcccag
     8341 ggcaaaaccc taggcgtaaa tatggtccga cgcggagtcc gctcattgtc gaacaaaata
     8401 aaacaaaaaa caaaacaaat tggaaacaga cctggacctt caagaggtgt tcaaggattt
     8461 atctttttct ttttgttcaa cattttgact ggaaaaaaga tcacagccca cctaaagagg
     8521 ttgtggaaaa tgctggaccc aagacaaggc ttggctgttc taaggaaagt caagagagtg
     8581 gtggccagtt tgatgagagg attgtcctca aggaaacgcc gttcccatga tgttctgact
     8641 gtgcaattcc taattttggg aatgctgttg atgacgggtg gagtgacctt ggtgcggaaa
     8701 aacagatggt tgctcctaaa tgtgacatct gaggacctcg ggaaaacatt ctctgtgggc
     8761 acaggcaact gcacaacaaa cattttggaa gccaagtact ggtgcccaga ctcaatggaa
     8821 tacaactgtc ccaatctcag tccaagagag gagccagatg acattgattg ctggtgctat
     8881 ggggtggaaa acgttagagt cgcatatggt aagtgtgact cagcaggcag gtctaggagg
     8941 tcaagaaggg ccattgactt gcctacgcat gaaaaccatg gtttgaagac ccggcaagaa
     9001 aaatggatga ctggaagaat gggtgaaagg caactccaaa agattgagag atggttcgtg
     9061 aggaacccct tttttgcagt gacggctctg accattgcct accttgtggg aagcaacatg
     9121 acgcaacgag tcgtgattgc cctactggtc ttggctgttg gtccggccta ctcagctcac
     9181 tgcattggaa ttactgacag ggatttcatt gagggggtgc atggaggaac ttgggtttca
     9241 gctaccctgg agcaagacaa gtgtgtcact gttatggccc ctgacaagcc ttcattggac
     9301 atctcactag agacagtagc cattgataga cctgctgagg tgaggaaagt gtgttacaat
     9361 gcagttctca ctcatgtgaa gattaatgac aagtgcccca gcactggaga ggcccaccta
     9421 gccgaagaga acgaagggga caatgcgtgc aagcgcactt attctgatag aggctggggc
     9481 aatggctgtg gcctatttgg gaaagggagc attgtggcat gcgccaaatt cacttgtgcc
     9541 aaatccatga gtttgtttga ggttgatcag accaaaattc agtatgtcat cagagcacaa
     9601 ttgcatgtag gggccaagca ggaaaattgg aataccgaca ttaagactct caagtttgat
     9661 gccctgtcag gctcccagga agtcgagttc attgggtatg gaaaagctac actggaatgc
     9721 caggtgcaaa ctgcggtgga ctttggtaac agttacatcg ctgagatgga aacagagagc
     9781 tggatagtgg acagacagtg ggcccaggac ttgaccctgc catggcagag tggaagtggc
     9841 ggggtgtgga gagagatgca tcatcttgtc gaatttgaac ctccgcatgc cgccactatc
     9901 agagtactgg ccctgggaaa ccaggaaggc tccttgaaaa cagctcttac tggcgcaatg
     9961 agggttacaa aggacacaaa tgacaacaac ctttacaaac tacatggtgg acatgtttct
    10021 tgcagagtga aattgtcagc tttgacactc aaggggacat cctacaaaat atgcactgac
    10081 aaaatgtttt ttgtcaagaa cccaactgac actggccatg gcactgttgt gatgcaggtg
    10141 aaagtgtcaa aaggagcccc ctgcaggatt ccagtgatag tagctgatga tcttacagcg
    10201 gcaatcaata aaggcatttt ggttacagtt aaccccatcg cctcaaccaa tgatgatgaa
    10261 gtgctgattg aggtgaaccc accttttgga gacagctaca ttatcgttgg gagaggagat
    10321 tcacgtctca cttaccagtg gcacaaagag ggaagctcaa taggaaagtt gttcactcag
    10381 accatgaaag gcgtggaacg cctggccgtc atgggagaca ccgcctggga tttcagctcc
    10441 gctggagggt tcttcacttc ggttgggaaa ggaattcata cggtgtttgg ctctgccttt
    10501 caggggctat ttggcggctt gaactggata acaaaggtca tcatgggggc ggtacttata
    10561 tgggttggca tcaacacaag aaacatgaca atgtccatga gcatgatctt ggtaggagtg
    10621 atcatgatgt ttttgtctct aggagttggg gcggatcaag gatgcgccat caactttggc
    10681 aagagagagc tcggatcacg gtccgggaca cgcgtgtaca tggacgcagt ctttgaatac
    10741 accatagact gcgatggatc tatcttgggt gcagcggtga acggaaaaaa gagtgcccat
    10801 ggctctccaa cattttggat gggaagtcat gaagtaaatg ggacatggat gatccacacc
    10861 ttggaggcat tagattacaa ggagtgtgag tggccactga cacatacgat tggaacatca
    10921 gttgaagaga gtgaaatgtt catgccgaga tcaatcggag gcccagttag ctctcacaat
    10981 catatccctg gatacaaggt tcagacgaac ggaccttgga tgcaggtacc actagaagtg
    11041 aagagagaag cttgcccagg gactagcgtg atcattgatg gcaactgtga tggacgggga
    11101 aaatcaacca gatccaccac ggatagcggg aaagttattc ctgaatggtg ttgccgctcc
    11161 tgcacaatgc cgcctgtgag cttccatggt agtgatgggt gttggtatcc catggaaatt
    11221 aggccaagga aaacgcatga aagccatctg gtgcgctcct gggttacagc tggagaaata
    11281 catgctgtcc cttttggttt ggtgagcatg atgatagcaa tggaagtggt cctaaggaaa
    11341 agacagggac caaagcaaat gttggttgga ggagtagtgc tcttgggagc aatgctggtc
    11401 gggcaagtaa ctctccttga tttgctgaaa ctcacagtgg ctgtgggatt gcatttccat
    11461 gagatgaaca atggaggaga cgccatgtat atggcgttga ttgctgcctt ttcaatcaga
    11521 ccagggctgc tcatcggctt tgggctcagg accctatgga gccctcggga acgccttgtg
    11581 ctgaccctag gagcagccat ggtggagatt gccttgggtg gcgtgatggg cggcctgtgg
    11641 aagtatctaa atgcagtttc tctctgcatc ctgacaataa atgctgttgc ttctaggaaa
    11701 gcatcaaata ccatcttgcc cctcatggct ctgttgacac ctgtcactat ggctgaggtg
    11761 agacttgccg caatgttctt ttgtgccatg gttatcatag gggtccttca ccagaatttc
    11821 aaggacacct ccatgcagaa gactatacct ctggtggccc tcacactcac atcttacctg
    11881 ggcttgacac aacctttttt gggcctgtgt gcatttctgg caacccgcat atttgggcga
    11941 aggagtatcc cagtgaatga ggcactcgca gcagctggtc tagtgggagt gctggcagga
    12001 ctggcttttc aggagatgga gaacttcctt ggtccgattg cagttggagg actcctgatg
    12061 atgctggtta gcgtggctgg gagggtggat gggctagagc tcaagaagct tggtgaagtt
    12121 tcatgggaag aggaggcgga gatcagcggg agttccgccc gctatgatgt ggcactcagt
    12181 gaacaagggg agttcaagct gctttctgaa gagaaagtgc catgggacca ggttgtgatg
    12241 acctcgctgg ccttggttgg ggctgccctc catccatttg ctcttctgct ggtccttgct
    12301 gggtggctgt ttcatgtcag gggagcgcgc agaagtggtc accggagaag tggggatgtc
    12361 ttgtgggata ttcccactcc taagatcatc gaggaatgtg aacatctgga ggatgggatt
    12421 tatggcatat tccagtcaac cttcttgggg gcctcccagc gaggagtggg agtggcacag
    12481 ggaggggtgt tccacacaat gtggcatgtc acaagaggag ctttccttgt caggaatggc
    12541 aagaagttga ttccatcttg ggcttcagta aaggaagacc ttgtcgccta tggtggctca
    12601 tggaagttgg aaggcagatg ggatggagag gaagaggtcc agttgatcgc ggctgttcca
    12661 ggaaagaacg tggtcaacgt ccagacaaaa ccgagcttgt tcaaagtgag gaatggggga
    12721 gaaatcgggg ctgtcgctct tgactatccg agtggcactt caggatctcc tattgttaac
    12781 aggaacggag aggtgattgg gctgtacggc aatggcatcc ttgtcggtga caactccttc
    12841 gtgtccgcca tatcccagac tgaggtgaag gaagaaggaa aggaggagct ccaagagatc
    12901 ccgacaatgc taaagaaagg aatgacaact gtccttgatt ttcatcctgg agctgggaag
    12961 acaagacgtt tcctcccaca gatcttggcc gagtgcgcac ggagacgctt gcgcactctt
    13021 gtgttggccc ccaccagggt tgttctttct gaaatgaagg aggcttttca cggcctggac
    13081 gtgaaattcc acacacaggc tttttccgct cacggcagcg ggagagaagt cattgatgcc
    13141 atgtgccatg ccaccctaac ttacaggatg ttggaaccaa ctagggttgt taactgggaa
    13201 gtgatcatta tggatgaagc ccattttttg gatccagcta gcatagccgc tagaggttgg
    13261 gcagcgcaca gagctagggc aaatgaaagt gcaacaatct tgatgacagc cacaccgcct
    13321 gggactagtg atgaatttcc acattcaaat ggtgaaatag aagatgttca aacggacata
    13381 cccagtgagc cctggaacac agggcatgac tggatcctgg ctgacaaaag gcccacggca
    13441 tggttccttc catccatcag agctgcaaat gtcatggctg cctctttgcg taaggctgga
    13501 aagagtgtgg tggtcctgaa caggaaaacc tttgagagag aataccccac gataaagcag
    13561 aagaaacctg actttatatt ggccactgac atagctgaaa tgggagccaa cctttgcgtg
    13621 gagcgagtgc tggattgcag gacggctttt aagcctgtgc ttgtggatga agggaggaag
    13681 gtggcaataa aagggccact tcgtatctcc gcatcctctg ctgctcaaag gagggggcgc
    13741 attgggagaa atcccaacag agatggagac tcatactact attctgagcc tacaagtgaa
    13801 aataatgccc accacgtctg ctggttggag gcctcaatgc tcttggacaa catggaggtg
    13861 aggggtggaa tggtcgcccc actctatggc gttgaaggaa ctaaaacacc agtttcccct
    13921 ggtgaaatga gactgaggga tgaccagagg aaagtcttca gagaactagt gaggaattgt
    13981 gacctgcccg tttggctttc gtggcaagtg gccaaggctg gtttgaagac gaatgatcgt
    14041 aagtggtgtt ttgaaggccc tgaggaacat gagatcttga atgacagcgg tgaaacagtg
    14101 aagtgcaggg ctcctggagg agcaaagaag cctctgcgcc caaggtggtg tgatgaaagg
    14161 gtgtcatctg accagagtgc gctgtctgaa tttattaagt ttgctgaagg taggagggga
    14221 gctgctgaag tgctagttgt gctgagtgaa ctccctgatt tcctggctaa aaaaggtgga
    14281 gaggcaatgg ataccatcag tgtgttcctc cactctgagg aaggctctag ggcttaccgc
    14341 aatgcactat caatgatgcc tgaggcaatg acaatagtca tgctgtttat actggctgga
    14401 ctactgacat cgggaatggt catctttttc atgtctccca aaggcatcag tagaatgtct
    14461 atggcgatgg gcacaatggc cggctgtgga tatctcatgt tccttggagg cgtcaaaccc
    14521 actcacatct cctatgtcat gctcatattc tttgtcctga tggtggttgt gatccccgag
    14581 ccagggcaac aaaggtccat ccaagacaac caagtggcat acctcattat tggcatcctg
    14641 acgctggttt cagcggtggc agccaacgag ctaggcatgc tggagaaaac caaagaggac
    14701 ctctttggga agaagaactt aattccatct agtgcttcac cctggagttg gccggatctt
    14761 gacctgaagc caggagctgc ctggacagtg tacgttggca ttgttacaat gctctctcca
    14821 atgttgcacc actggatcaa agtcgaatat ggcaacctgt ctctgtctgg aatagcccag
    14881 tcagcctcag tcctttcttt catggacaag gggataccat tcatgaagat gaatatctcg
    14941 gtcataatgc tgctggtcag tggctggaat tcaataacag tgatgcctct gctctgtggc
    15001 atagggtgcg ccatgctcca ctggtctctc attttacctg gaatcaaagc gcagcagtca
    15061 aagcttgcac agagaagggt gttccatggc gttgccaaga accctgtggt tgatgggaat
    15121 ccaacagttg acattgagga agctcctgaa atgcctgccc tttatgagaa gaaactggct
    15181 ctatatctcc ttcttgctct cagcctagct tctgttgcca tgtgcagaac gcccttttca
    15241 ttggctgaag gcattgtcct agcatcagct gccttagggc cgctcataga gggaaacacc
    15301 agccttcttt ggaatggacc catggctgtc tccatgacag gagtcatgag ggggaatcac
    15361 tatgcttttg tgggagtcat gtacaatcta tggaagatga aaactggacg ccgggggagc
    15421 gcgaatggaa aaactttggg tgaagtctgg aagagggaac tgaatctgtt ggacaagcga
    15481 cagtttgagt tgtataaaag gaccgacatt gtggaggtgg atcgtgatac ggcacgcagg
    15541 catttggccg aagggaaggt ggacaccggg gtggcggtct ccagggggac cgcaaagtta
    15601 aggtggttcc atgagcgtgg ctatgtcaag ctggaaggta gggtgattga cctggggtgt
    15661 ggccgcggag gctggtgtta ctacgctgct gcgcaaaagg aagtgagtgg ggtcaaagga
    15721 tttactcttg gaagagacgg ccatgagaaa cccatgaatg tgcaaagtct gggatggaac
    15781 atcatcacct tcaaggacaa aactgatatc caccgcctag aaccagtgaa atgtgacacc
    15841 cttttgtgtg acattggaga gtcatcatcg tcatcggtca cagaggggga aaggaccgtg
    15901 agagttcttg atactgtaga aaaatggctg gcttgtgggg ttgacaactt ctgtgtgaag
    15961 gtgttagctc catacatgcc agatgttctt gagaaactgg aattgctcca aaggaggttt
    16021 ggcggaacag tgatcaggaa ccctctctcc aggaattcca ctcatgaaat gtactacgtg
    16081 tctggagccc gcagcaatgt cacatttact gtgaaccaaa catcccgcct cctgatgagg
    16141 agaatgaggc gtccaactgg aaaagtgacc ctggaggctg acgtcatcct cccaattggg
    16201 acacgcagtg ttgagacaga caagggaccc ctggacaaag aggccataga agaaagggtt
    16261 gagaggataa aatctgagta catgacctct tggttttatg acaatgacaa cccctacagg
    16321 acctggcact actgtggctc ctatgtcaca aaaacctcag gaagtgcggc gagcatggta
    16381 aatggtgtta ttaaaattct gacatatcca tgggacagga tagaggaggt cacaagaatg
    16441 gcaatgactg acacaacccc ttttggacag caaagagtgt ttaaagaaaa agttgacacc
    16501 agagcaaagg atccaccagc gggaactagg aagatcatga aagttgtcaa caggtggctg
    16561 ttccgccacc tggccagaga aaagaacccc agactgtgca caaaggaaga atttattgca
    16621 aaagtccgaa gtcatgcagc cattggagct tacctggaag aacaagaaca gtggaagact
    16681 gccaatgagg ctgtccaaga cccaaagttc tgggaactgg tggatgaaga aaggaagctg
    16741 caccaacaag gcaggtgtcg gacttgtgtg tacaacatga tggggaaaag agagaagaag
    16801 ctgtcagagt ttgggaaagc aaagggaagc cgtgccatat ggtatatgtg gctgggagcg
    16861 cggtatcttg agtttgaggc cctgggattc ctgaatgagg accattgggc ttccagggaa
    16921 aactcaggag gaggagtgga aggcattggc ttacaatacc taggatatgt gatcagagac
    16981 ctggctgcaa tggatggtgg tggattctac gcggatgaca ccgctggatg ggacacgcgc
    17041 atcacagagg cagaccttga tgatgaacag gagatcttga actacatgag cccacatcac
    17101 aaaaaactgg cacaagcagt gatggaaatg acatacaaga acaaagtggt gaaagtgttg
    17161 agaccagccc caggagggaa agcctacatg gatgtcataa gtcgacgaga ccagagagga
    17221 tccgggcagg tagtgactta tgctctgaac accatcacca acttgaaagt ccaattgatc
    17281 agaatggcag aagcagagat ggtgatacat caccaacatg ttcaagattg tgatgaatca
    17341 gttctgacca ggctggaggc atggctcact gagcacggat gtgacagact gaagaggatg
    17401 gcggtgagtg gagacgactg tgtggtccgg cccatcgatg acaggttcgg cctggccctg
    17461 tcccatctca acgccatgtc caaggttaga aaggacatat ctgaatggca gccatcaaaa
    17521 gggtggaatg attgggagaa tgtgcccttc tgttcccacc acttccatga actacagctg
    17581 aaggatggca ggaggattgt ggtgccttgc cgagaacagg acgagctcat tgggagagga
    17641 agggtgtctc caggaaacgg ctggatgatc aaggaaacag cttgcctcag caaagcctat
    17701 gccaacatgt ggtcactgat gtattttcac aaaagggaca tgaggctact gtcattggct
    17761 gtttcctcag ctgttcccac ctcatgggtt ccacaaggac gcacaacatg gtcgattcat
    17821 gggaaagggg agtggatgac cacggaagac atgcttgagg tgtggaacag agtatggata
    17881 accaacaacc cacacatgca ggacaagaca atggtgaaaa aatggagaga tgtcccttat
    17941 ctaaccaaga gacaagacaa gctgtgcgga tcactgattg gaatgaccaa tagggccacc
    18001 tgggcctccc acatccattt agtcatccat cgtatccgaa cgctgattgg acaggagaaa
    18061 tacactgact acctaacagt catggacagg tattctgtgg atgctgacct gcaactgggt
    18121 gagcttatct gaaacaccat ctaacaggaa taaccgggat acaaaccacg ggtggagaac
    18181 cggactcccc acaacctgaa accgggatat aaaccacggc tggagaaccg gactccgcac
    18241 ttaaaatgaa acagaaaccg ggataaaaac tacggatgga gaaccggact ccacacattg
    18301 agacagaaga agttgtcagc ccagaacccc acacgagttt tgccactgct aagctgtgag
    18361 gcagtgcagg ctgggacagc cgacctccag gttgcgaaaa acctggtttc tgggacctcc
    18421 caccccagag taaaaagaac ggagcctccg ctaccaccct cccacgtggt ggtagaaaga
    18481 cggggtctag aggttagagg agaccctcca gggaacaaat agtgggacca tattgacgcc
    18541 agggaaagac cggagtggtt ctctgctttt cctccagagg tctgtgagca cagtttgctc
    18601 aagaataagc agacctttgg atgacaaaca caaaaccact cgagcaagac gtttcccgtt
    18661 gaatatggct cataacaccc cttgtattac tgtttatgta agcagacagt tttattgttc
    18721 atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac acaacgtggc
    18781 tttgttgaat aaatcgaact tttgctgagt tgaaggatca gatcacgcat cttcccgaca
    18841 acgcagaccg ttccgtggca aagcaaaagt tcaaaatcac caactggtcc acctacaaca
    18901 aagctctcat caaccgtggc tccctcactt tctggctgga tgatggggcg attcaggcct
    18961 ggtatgagtc agcaacacct tcttcacgag gcagacctca gcgctagcgg agtgtagtgg
    19021 gatccccggg taccgagctc gaattcgccc tatagtgagt cgtattacaa ttcactggcc
    19081 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
    19141 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
    19201 caacagttgc gcagctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc
    19261 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
    19321 agttaagcca gccccgacac ccgccaacac ccgctgacgc gaaccccttg cggccgcatc
    19381 gaatataact tcgtataatg tatgctatac gaagttatta gcgatgagct cggacttcca
    19441 ttgttcattc cacggacaaa aacagagaaa ggaaacgaca gaggccaaaa agctcgcttt
    19501 cagcacctgt cgtttccttt cttttcagag ggtattttaa ataaaaacat taagttatga
    19561 cgaagaagaa cggaaacgcc ttaaaccgga aaattttcat aaatagcgaa aacccgcgag
    19621 gtcgccgccc cgtaacctgt cggatcaccg gaaaggaccc gtaaagtgat aatgattatc
    19681 atctacatat cacaacgtgc gtggaggcca tcaaaccacg tcaaataatc aattatgacg
    19741 caggtatcgt attaattgat ctgcatcaac ttaacgtaaa agcaacttca gacaatacaa
    19801 atcagcgaca ctgaatacgg ggcaacctca tgtccgagct cgcgagctcg tcgacagcga
    19861 cacacttgca tcggatgcag cccggttaac gtgccggcac ggcctgggta accaggtatt
    19921 ttgtccacat aaccgtgcgc aaaatgttgt ggataagcag gacacagcag caatccacag
    19981 caggcataca accgcacacc gaggttactc cgttctacag gttacgacga catgtcaata
    20041 cttgcccttg acaggcattg atggaatcgt agtctcacgc tgatagtctg atcgacaata
    20101 caagtgggac cgtggtccca gaccgataat cagaccgaca acacgagtgg gatcgtggtc
    20161 ccagactaat aatcagaccg acgatacgag tgggaccgtg gtcccagact aataatcaga
    20221 ccgacgatac gagtgggacc gtggttccag actaataatc agaccgacga tacgagtggg
    20281 accgtggtcc cagactaata atcagaccga cgatacgagt gggaccatgg tcccagacta
    20341 ataatcagac cgacgatacg agtgggaccg tggtcccagt ctgattatca gaccgacgat
    20401 acgagtggga ccg
//
