LOCUS       sequence-357767-        9810 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EGFP driven by tinman transcription factor binding sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9810)
  AUTHORS   Stern DL
  TITLE     Transgenic tools for targeted chromosome rearrangements allow 
            construction of balancer chromosomes in non-melanogaster Drosophila 
            species.
  JOURNAL   G3 (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030.
  PUBMED    35143616
REFERENCE   2  (bases 1 to 9810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030."
COMMENT     Sequence Label: pBac{3Xtin-eGFP,w+,attB}
FEATURES             Location/Qualifiers
     source          1..9810
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    887..956
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(1181..1199)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1651..1722,2124..2397,2472..3126,3188..3503,3707..3838,
                     3909..4523)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     promoter        5635..5873
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     5820..5841
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          5888..5954
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     CDS             5985..6698
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     intron          6785..6850
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6980..7000
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7272..7406
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7309..7328)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7363..7382
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7693..7727
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7763..7781)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7848..7952
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7953..8021,8022..8813)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8171..8190)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8984..9572
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9473..9492
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   9639..9701
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 tgtttattta tttattaaaa aaaaacaaaa actcaaaatt tcttctataa agtaacaaaa
       61 cttttaaaca ttctctcttt tacaaaaata aacttatttt gtactttaaa aacagtcatg
      121 ttgtattata aaataagtaa ttagcttaac ttatacataa tagaaacaaa ttatacttat
      181 tagtcagtca gaaacaactt tggcacatat caatattatg ctctcgacaa ataacttttt
      241 tgcatttttt gcacgatgca tttgcctttc gccttatttt agaggggcag taagtacagt
      301 aagtacgttt tttcattact ggctcttcag tactgtcatc cgatgtacca ggcacttcat
      361 ttggcaaaat attagagata ttatcgcgca aatatctctt caaagtagga gcttctaaac
      421 gcttacgcat aaacgatgac gtcaggctca tgtaaaggtt tctcataaat tttttgcgac
      481 tttgaacctt ttctcccttg ctactgacat tatggctgta tataataaaa gaatttatgc
      541 aggcaatgtt tatcattccg tacaataatg ccataggcca cctattcgtc ttcctactgc
      601 aggtcatcac agaacacatt tggtctagcg tgtccactcc gcctttagtt tgattataat
      661 acataaccat ttgcggttta ccggtacttt cgttgataga agcatcctca tcacaagatg
      721 ataataagta taccatctta gctggcttcg gtttatatga gacgagagta aggggtccgt
      781 caaaacaaaa catcgatgtt cccactggcc tggagcgact gtttttcagt acttccggta
      841 tctcgcgttt gtttgatcgc acggttccca cgatgtaggt cacggtctcg aagccgcggt
      901 gcgggtgcca gggcgtgccc ttgggctccc cgggcgcgta ctccacctca cccatctggt
      961 ccatcatgat gaacgggtcg aggtggcggt agttgatccc ggcgaacgcg cggcgcaccg
     1021 ggaagccctc gccctcgaaa ccgctgggcg cggtggtcac ggtgagcacg ggacgtgcga
     1081 cggcgtcggc gggtgcggat acgcggggca gcgtcagcgg gttctcgacg gtcacggcgg
     1141 gcatgtcgac aagccgaaca tatgggcgcg cctagtatgt atgtaagtta ataaaaccca
     1201 tttttgcgga aagtagataa aaaaaacatt tttttttttt actgcactgg atatcattga
     1261 acttatctga tcagttttaa atttacttcg atccaagggt atttgatgta ccaggttctt
     1321 tcgattacct ctcactcaaa atgacattcc actcaaagtc agcgctgttt gcctccttct
     1381 ctgtccacag aaatatcgcc gtctctttcg ccgctgcgtc cgctatctct ttcgccaccg
     1441 tttgtagcgt tacgtagcgt caatgtccgc cttcagttgc attttgtcag cggtttcgtg
     1501 acgaagctcc aagcggttta cgccatcaat taaacacaaa gtgctgtgcc aaaactcctc
     1561 tcgcttctta tttttgtttg ttttttgagt gattggggtg gtgattggtt ttgggtgggt
     1621 aagcagggga aagtgtgaaa aatcccggca atgggccaag aggatcagga gctattaatt
     1681 cgcggaggca gcaaacaccc atctgccgag catctgaaca atgtgagtag tacatgtgca
     1741 tacatcttaa gttcacttga tctataggaa ctgcgattgc aacatcaaat tgtctgcggc
     1801 gtgagaactg cgacccacaa aaatcccaaa ccgcaattgc acaaacaaat agtgacacga
     1861 aacagattat tctggtagct gttctcgcta tataagacaa tttttgagat catatcatga
     1921 tcaagacatc taaaggcatt cattttcgac tatattcttt tttacaaaaa atataacaac
     1981 cagatatttt aagctgatcc tagatgcaca aaaaataaat aaaagtataa acctacttcg
     2041 taggatactt cggggtactt tttgttcggg gttagatgag cataacgctt gtagttgata
     2101 tttgagatcc cctatcattg cagggtgaca gcggagcggc ttcgcagagc tgcattaacc
     2161 agggcttcgg gcaggccaaa aactacggca cgctccggcc acccagtccg ccggaggact
     2221 ccggttcagg gagcggccaa ctagccgaga acctcaccta tgcctggcac aatatggaca
     2281 tctttggggc ggtcaatcag ccgggctccg gatggcggca gctggtcaac cggacacgcg
     2341 gactattctg caacgagcga cacataccgg cgcccaggaa acatttgctc aagaacggtg
     2401 agtttctatt cgcagtcggc tgatctgtgt gaaatcttaa taaagggtcc aattaccaat
     2461 ttgaaactca gtttgcggcg tggcctatcc gggcgaactt ttggccgtga tgggcagttc
     2521 cggtgccgga aagacgaccc tgctgaatgc ccttgccttt cgatcgccgc agggcatcca
     2581 agtatcgcca tccgggatgc gactgctcaa tggccaacct gtggacgcca aggagatgca
     2641 ggccaggtgc gcctatgtcc agcaggatga cctctttatc ggctccctaa cggccaggga
     2701 acacctgatt ttccaagcca tggtgcggat gccacgacat ctgacctatc ggcagcgagt
     2761 ggcccgcgtg gatcaggtga tccaggagct ttcgctcagc aaatgtcagc acacgatcat
     2821 cggtgtgccc ggcagggtga aaggtctgtc cggcggagaa aggaagcgtc tggcattcgc
     2881 ctccgaggct ctaaccgatc cgccgcttct gatctgcgat gagcccacct ccggactgga
     2941 ctcctttacc gcccacagcg tcgtccaggt gctgaagaag ctgtcgcaga agggcaagac
     3001 cgtcatcctg accattcatc agccgtcttc cgagctgttt gagctctttg acaagatcct
     3061 tctgatggcc gagggcaggg tagctttctt gggcactccc agcgaagccg tcgacttctt
     3121 ttcctagtga gttcgatgtg tttattaagg gtatctagta ttacataaca tctcaactcc
     3181 tatccagcgt gggtgcccag tgtcctacca actacaatcc ggcggacttt tacgtacagg
     3241 tgttggccgt tgtgcccgga cgggagatcg agtcccgtga tcggatcgcc aagatatgcg
     3301 acaattttgc cattagcaaa gtagcccggg atatggagca gttgttggcc accaaaaatc
     3361 tggagaagcc actggagcag ccggagaatg ggtacaccta caaggccacc tggttcatgc
     3421 agttccgggc ggtcctgtgg cgatcctggc tgtcggtgct caaggaacca ctcctcgtaa
     3481 aagtgcgact tattcagaca acggtgagtg gttccagtgg aaacaaatga tataacgctt
     3541 acaattcttg gaaacaaatt cgctagattt tagatagaat tgcctgattc cacacccttc
     3601 ttagtttttt tcaatgagat gtatagttta tagttttgca gaagataaat aaatttcatt
     3661 taactcgcga atattaatga gatgcgagta acattttaat ttgcagatgg ttgccatctt
     3721 gattggcctc atctttttgg gccaacaact cacgcaagtg ggtgtgatga atatcaacgg
     3781 agccatcttc ctcttcctga ccaacatgac ctttcaaaac gtctttgcca cgataaatgt
     3841 aagtcatgtt tagaatacat ttgcatttca ataatttact aactttctaa tgaatcgatt
     3901 cgatttaggt gttcacctca gagctgccag tttttatgag ggaggcccga agtcgacttt
     3961 atcgctgtga cacatacttt ctgggcaaaa cgattgccga attgccgctt tttctcacag
     4021 tgccactggt cttcacggcg attgcctatc cgatgatcgg actgcgggcc ggagtgctgc
     4081 acttcttcaa ctgcctggcg ctggtcactc tggtggccaa tgtgtcaacg tccttcggat
     4141 atctaatatc ctgcgccagc tcctcgacct cgatggcgct gtctgtgggt ccgccggtta
     4201 tcataccatt cctgctcttt ggcggcttct tcttgaactc gggctcggtg ccagtatacc
     4261 tcaaatggtt gtcgtacctc tcatggttcc gttacgccaa cgagggtctg ctgattaacc
     4321 aatgggcgga cgtggagccg ggcgaaatta gctgcacatc gtcgaacacc acgtgcccca
     4381 gttcgggcaa ggtcatcctg gagacgctta acttctccgc cgccgatctg ccgctggact
     4441 acgtgggtct ggccattctc atcgtgagct tccgggtgct cgcatatctg gctctaagac
     4501 ttcgggcccg acgcaaggag tagccgacat atatccgaaa taactgcttg tttttttttt
     4561 ttaccattat taccatcgtg tttactgttt attgccccct caaaaagcta atgtaattat
     4621 atttgtgcca ataaaaacaa gatatgacct atagaataca agtatttccc cttcgaacat
     4681 ccccacaagt agactttgga tttgtcttct aaccaaaaga cttacacacc tgcatacctt
     4741 acatcaaaaa ctcgtttatc gctacataaa acaccgggat atatttttta tatacatact
     4801 tttcaaatcg cgcgccctct tcataattca cctccaccac accacgtttc gtagttgctc
     4861 tttcgctgtc tcccacccgc tctccgcaac acattcacct tttgttcgac gaccttggag
     4921 cgactgtcgt tagttccgcg cgattcggtt cgctcaaatg gttccgagtg gttcatttcg
     4981 tctcaataga aattagtaat aaatatttgt atgtacaatt tatttgctcc aatatatttg
     5041 tatatatttc cctcacagct atatttattc taatttaata ttatgacttt ttaaggtaat
     5101 tttttgtgac ctgttcggag tgattagcgt tacaatttga actgaaagtg acatccagtg
     5161 tttgttcctt gtgtagatgc atctcaaaaa aatggtgggc ataatagtgt tgtttatata
     5221 tatcaaaaat aacaactata ataataagaa tacatttaat ttagaaaatg cttggatttc
     5281 actggaacta gggcgcgcct ccggaacata atggtgcagg gcgctgactt ccgcgtttcc
     5341 agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt
     5401 ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta
     5461 aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt
     5521 ggccagggcc gcacatatgc aatggttaat tcgagctcgc ccggggctct caagtgtgca
     5581 ctcaagtgtg ctctcaagtg tgatccaagc ttatcgattt cgaaccctcg accgccggag
     5641 tataaataga ggcgcttcgt ctacggagcg acaattcaat tcaaacaagc aaagtgaaca
     5701 cgtcgctaag cgaaagctaa gcaaataaac aagcgcagct gaacaagcta aacaatctgc
     5761 agtaaagtgc aagttaaagt gaatcaatta aaagtaacca gcaaccaagt aaatcaactg
     5821 caactactga aatctgccaa gaagtaatta ttgaatacaa gaagagaact ctgaatagat
     5881 ctaaaaggta ggttcaacca ctgatgccta ggcacaccga aacgactaac cctaattctt
     5941 atcctttact tcaggcggcc gcggctcgag ggtaccaatc aaacatgtcc aaaggtgaag
     6001 aactgtttac cggagtagtc ccgatattgg ttgaactcga cggcgatgtc aacggtcata
     6061 aattcagtgt gtccggcgag ggtgagggcg acgccacata cggtaagctg acgttgaagt
     6121 tcatatgcac cacgggcaag ctgcccgtgc catggccgac gttggtcacg acgctgacgt
     6181 atggtgtcca gtgtttcagc cgttaccccg atcatatgaa gcagcatgac tttttcaagt
     6241 cggcgatgcc ggagggatac gttcaagaga ggaccatttt cttcaaggat gacggcaact
     6301 ataagacgcg agcggaggtg aaatttgaag gcgacacact cgttaaccgt attgagttga
     6361 agggcattga ttttaaggag gatggcaaca ttctgggcca taagttggaa tacaactaca
     6421 actcgcataa tgtgtatata atggcagata agcagaaaaa cggaataaag gttaacttca
     6481 agattcgcca caacatagag gacggttccg tgcaacttgc agatcattac caacagaaca
     6541 cacccattgg agatggccca gttctcttgc cagacaatca ctacctttcc acacagtccg
     6601 cgttgagcaa ggaccccaat gaaaagcggg accacatggt gttgctggag tttgtgaccg
     6661 cagctggtat tacacacggc atggatgagc tctacaagta atctagagga tctttgtgaa
     6721 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc
     6781 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg
     6841 tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa
     6901 tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga
     6961 ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc
     7021 ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt
     7081 tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata
     7141 tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt
     7201 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     7261 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     7321 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     7381 tcaatgtatc ttatcatgtc tggatcgatc tggccggcga tatctataac aagaaaatat
     7441 atatataata agttatcacg taagtagaac atgaaataac aatataatta tcgtatgagt
     7501 taaatcttaa aagtcacgta aaagataatc atgcgtcatt ttgactcacg cggtcgttat
     7561 agttcaaaat cagtgacact taccgcattg acaagcacgc ctcacgggag ctccaagcgg
     7621 cgactgagat gtcctaaatg cacagcgacg gattcgcgct atttagaaag agagagcaat
     7681 atttcaagaa tgcatgcgtc aattttacgc agactatctt tctaggggcc ggccgtttaa
     7741 acgaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     7801 ataataatgg tttcttagac tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     7861 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     7921 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     7981 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     8041 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     8101 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     8161 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     8221 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     8281 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     8341 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     8401 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     8461 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     8521 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     8581 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     8641 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     8701 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     8761 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     8821 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     8881 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     8941 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     9001 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     9061 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     9121 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     9181 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     9241 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     9301 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     9361 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     9421 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     9481 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     9541 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     9601 tcctggcctt ttgctggcct tttgctcaca tgttaccgcc ctagaaagat aatcatattg
     9661 tgacgtacgt taaagataat catgcgtaaa attgacgcat gtgttttatc ggtctgtata
     9721 tcgaggttta tttattaatt tgaatagata ttaagtttta ttatatttac acttacatac
     9781 taataataaa ttcaacaaac aatttattta
//
