LOCUS       sequence-357793-        6182 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  fluorescent reporter gene with yeast recombination site within 
            intron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6182)
  AUTHORS   Stern DL
  TITLE     Transgenic tools for targeted chromosome rearrangements allow 
            construction of balancer chromosomes in non-melanogaster Drosophila 
            species.
  JOURNAL   G3 (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030.
  PUBMED    35143616
REFERENCE   2  (bases 1 to 6182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Feb 10. pii: 6526388. doi: 
            10.1093/g3journal/jkac030."
COMMENT     Sequence Label: p{Mhc-F4-678-DsRed::KD-RC,attB}
FEATURES             Location/Qualifiers
     source          1..6182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    306..339
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    380..449
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(668..688)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(668..686)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(707..723)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(707..723)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(720..742)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    731..747
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(755..761,762..779,780..785))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    800..821
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(938..955)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1109..1697)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1189..1208)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1868..2659,2660..2728))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2491..2510
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2729..2833)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2860..3315
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2947..2966)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3157..3178
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3441..3463
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3455..3472
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3456..3472
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3482..3501
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3482..3500
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4648..4672
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4770..4794)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 attctctggg acgatgagtc attcctttcg gggccacagc ataatcgttg ccagctcacc
       61 gaaatggtga cttcatttct taactgccgt caagcatgcg attgtacata catacatatt
      121 tatatatgta catatttatg tgactatggt aggtcgatat aatagcaatc aacgcaagca
      181 aatgtgtcag tcctgcttac aggaacgatt ctatttagta attttcgttg tataaagtaa
      241 ttatgtatgt atgtaagccc cataaatctg aaacaattag gcaaaaccat gcgaagctct
      301 ctagtataac ttcgtataat gtatgctata cgaagttctc gagggggggc ccggtaccgt
      361 cgacgatgta ggtcacggtc tcgaagccgc ggtgcgggtg ccagggcgtg cccttgggct
      421 ccccgggcgc gtactccacc tcacccatct ggtccatcat gatgaacggg tcgaggtggc
      481 ggtagttgat cccggcgaac gcgcggcgcg ccgggaagcc ctcgccctcg aaaccgctgg
      541 gcgcggtggt cacggtgagc acgggacgtg cgacggcgtc ggcgggtgcg gatacgcggg
      601 gcagcgtcag cgggttctcg acggtcacgg cgggcatgtc gacactagtg gtacccagct
      661 tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc
      721 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
      781 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
      841 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
      901 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
      961 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     1021 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     1081 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     1141 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     1201 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     1261 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     1321 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1381 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     1441 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     1501 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     1561 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     1621 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     1681 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     1741 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     1801 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     1861 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     1921 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     1981 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     2041 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     2101 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     2161 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     2221 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     2281 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     2341 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     2401 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     2461 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     2521 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     2581 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     2641 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     2701 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     2761 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     2821 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat
     2881 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     2941 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     3001 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     3061 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     3121 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     3181 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     3241 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     3301 taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     3361 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     3421 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc
     3481 gtaatacgac tcactatagg gcgaattgga gctccaccgc ggtggcggcc gctttaatat
     3541 actcgaattc gttgtcggct catatacatg ggcgaaataa tttcgaatat gttttaaaaa
     3601 taaccaaaga cattagaaag agatcgccaa tacttataca ttatgtctat gtgtgccatg
     3661 tggtagcatg agccaaaaag cttctcgaaa ttacgaatta catatagacg aatatgtatg
     3721 tgactttagt ttcgaaataa tttcgagaat tttaaaaata acgcattcgt taaaagttcg
     3781 cgtcaattcg aatcgaattt tcgattgtcg atttagtgtg gattgtcgaa aatcgttccg
     3841 ccttcgaagt ttactgaaag gaatcattgc gatctcgtga attgcttgta tgagaacacg
     3901 ccaccatatc gagatacctg ttcaaaatat cgaaatcgcg ttccaaacgg tagtagttac
     3961 cagttgagtg agttgtggtg acagtttaac ctctctacat ttgtaataat aagcattgtt
     4021 ttggcgtatc aatagtctgt agattttttc acaaagaaac ctggagaaat gtacacaaat
     4081 atctaaatga aaattggcgt tacgtaacaa ataccaacgc cccgaaaatc cgtatatatg
     4141 caaagcattc tgatatatat atataagtgg tatacatata catattcgta tcggatccgt
     4201 gatctatatt taggattaaa accctcgacc gccggagtat aaatagaggc gcttcgtcta
     4261 cggagcgaca attcaattca aacaagcaaa gtgaacacgt cgctaagcga aagctaagca
     4321 aataaacaag cgcagctgaa caagctaaac aatcgggcgg ccgcactaga gccggtcgcc
     4381 accatgaggt cttccaagaa tgttatcaag gagttcatga ggtttaaggt tcgcatggaa
     4441 ggaacggtca atgggcacga gtttgaaata gaaggcgaag gagaggggag gccatacgaa
     4501 ggccacaata ccgtaaagct taaggtgagt agtacatgtg catacatctt aagttcactt
     4561 gatctatagg aactgcgatt gcaacatcaa attgtctgcg gcgtgagaac tgcgacccac
     4621 aaaaatccca aaccgcggcc gtttatcacc actttgtaca agaaagctgg gtcggcgcgc
     4681 ccacccttaa acgatatcag acatttgtct gataatgctt cattatcaga caaatgtctg
     4741 atatcgtttg gtgaaggggg cggccgcgga gcctgctttt ttgtacaaac ttgtgataaa
     4801 cgaattcttg aagacgaaag ggcacaaaca aatagtgaca cgaaacagat tattctggta
     4861 gctgttctcg ctatataaga caatttttga gatcatatca tgatcaagac atctaaaggc
     4921 attcattttc gactatattc ttttttacaa aaaatataac aaccagatat tttaagctga
     4981 tcctagatgc acaaaaaata aataaaagta taaacctact tcgtaggata cttcggggta
     5041 ctttttgttc ggggttagat gagcataacg cttgtagttg atatttgaga tcccctatca
     5101 ttgcaggtaa ccaagggggg acctttgcca tttgcttggg atattttgtc accacaattt
     5161 cagtatggaa gcaaggtata tgtcaagcac cctgccgaca taccagacta taaaaagctg
     5221 tcatttcctg aaggatttaa atgggaaagg gtcatgaact ttgaagacgg tggcgtcgtt
     5281 actgtaaccc aggattccag tttgcaggat ggctgtttca tctacaaggt caagttcatt
     5341 ggcgtgaact ttccttccga tggacctgtt atgcaaaaga agacaatggg ctgggaagcc
     5401 agcactgagc gtttgtatcc tcgtgatggc gtgttgaaag gagagattca taaggctctg
     5461 aagctgaaag acggtggtca ttacctagtt gaattcaaaa gtatttacat ggcaaagaag
     5521 cctgtgcagc taccagggta ctactatgtt gactccaaac tggatataac aagccacaac
     5581 gaagactata caatcgttga gcagtatgaa agaaccgagg gacgccacca tctgttcctt
     5641 tagcggccat cgaattcgag ctcgcccact aagcgtcgcg ccacttcaac gctcgatggg
     5701 agcgtcattg gtgggcgggg taaccgtcga aatcagtgtt tacgcttcca atcgcaacaa
     5761 aaaattcact gcaacactga aaagcatacg aaaacgatga agattgtacg agaaaccata
     5821 aagtatttta tccacaaaga cacgtatagc agaaaagcca agttaactcg gcgataagtt
     5881 gtgtacacaa gaataaaatc ggccagattc agtgttgtca gaaataagaa aaccccacta
     5941 tgtttttctt tgccttttct ttctcccagc gatcattcat ttcgtggtga aagaacgggg
     6001 tcattgcacg gagtttcgac tgcgggaaag cagagctgcc gttcacttcg tctataatta
     6061 gcgctttcta ttttccccga ttcgggccgc tgctgcgctt ttccgcctgc tgtttgtggc
     6121 aagtgtagca gcaggctgtg cacgcagtgt ggcatgcact tggctttcca ccgttggtat
     6181 cg
//
