LOCUS       sequence-357914-       10814 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  to epress PCP-GFP fusion protein in the early fly embryos (driven by
            maternal nanos driver).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10814)
  AUTHORS   Liu J, Hansen D, Eck E, Kim YJ, Turner M, Alamos S, Garcia HG
  TITLE     Real-time single-cell characterization of the eukaryotic 
            transcription cycle reveals correlations between RNA initiation, 
            elongation, and cleavage.
  JOURNAL   PLoS Comput Biol. 2021 May 18;17(5):e1008999. doi: 
            10.1371/journal.pcbi.1008999. eCollection 2021 May.
  PUBMED    34003867
REFERENCE   2  (bases 1 to 10814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Comput
            Biol."; date: "2021-05-18"; volume: "17(5):e1008999. doi"; pages: " 
            10.1371/journal.pcbi.1008999. eCollection 2021 May"
COMMENT     Sequence Label: pCasper-pNos-PCP-eGFP
FEATURES             Location/Qualifiers
     source          1..10814
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1760..1778
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     primer_bind     1807..1823
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1808..1824
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             join(3154..3156,3157..3870)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(3154..3156,3157..3867)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3196..3217)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3457..3476)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3804..3825
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4698..4715)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4698..4714)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4707..4729)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    join(4892..5093,5094..5124)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(5662..5681)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5781..5803
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5841..5859)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5927..6031
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6032..6100,6101..6892)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6250..6269)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7063..7651
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7552..7571
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    join(7886..7916,7917..8471)
                     /label=P element 5' end
     CDS             complement(join(9250..9864,9935..10066,10270..10585,
                     10647..10814,1..487,562..835,1237..1308))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
ORIGIN
        1 ctgtgggcgg taaaggagtc cagtccggag gtgggctcat cgcagatcag aagcggcgga
       61 tcggttagag cctcggaggc gaatgccaga cgcttccttt ctccgccgga cagacctttc
      121 accctgccgg gcacaccgat gatcgtgtgc tgacatttgc tgagcgaaag ctcctggatc
      181 acctgatcca cgcgggccac tcgctgccga taggtcagat gtcgtggcat ccgcaccatg
      241 gcttggaaaa tcaggtgttc cctggccgtt agggagccga taaagaggtc atcctgctgg
      301 acataggcgc acctggcctg catctccttg gcgtccacag gttggccatt gagcagtcgc
      361 atcccggatg gcgatacttg gatgccctgc ggcgatcgaa aggcaagggc attcagcagg
      421 gtcgtctttc cggcaccgga actgcccatc acggccaaaa gttcgcccgg ataggccacg
      481 ccgcaaactg agtttcaaat tggtaattgg accctttatt aagatttcac acagatcagc
      541 cgactgcgaa tagaaactca ccgttcttga gcaaatgttt cctgggcgcc ggtatgtgtc
      601 gctcgttgca gaatagtccg cgtgtccggt tgaccagctg ccgccatccg gagcccggct
      661 gattgaccgc cccaaagatg tccatattgt gccaggcata ggtgaggttc tcggctagtt
      721 ggccgctccc tgaaccggag tcctccggcg gactgggtgg ccggagcgtg ccgtagtttt
      781 tggcctgccc gaagccctgg ttaatgcagc tctgcgaagc cgctccgctg tcaccctgca
      841 atgatagggg atctcaaata tcaactacaa gcgttatgct catctaaccc cgaacaaaaa
      901 gtaccccgaa gtatcctacg aagtaggttt atacttttat ttattttttg tgcatctagg
      961 atcagcttaa aatatctggt tgttatattt tttgtaaaaa agaatatagt cgaaaatgaa
     1021 tgcctttaga tgtcttgatc atgatatgat ctcaaaaatt gtcttatata gcgagaacag
     1081 ctaccagaat aatctgtttc gtgtcactat ttgtttgtgc aattgcggtt tgggattttt
     1141 gtgggtcgca gttctcacgc cgcagacaat ttgatgttgc aatcgcagtt cctatagatc
     1201 aagtgaactt aagatgtatg cacatgtact actcacattg ttcagatgct cggcagatgg
     1261 gtgtttgctg cctccgcgaa ttaatagctc ctgatcctct tggcccattg ccgggatttt
     1321 tcacactttc ccctgcttac ccacccaaaa ccaatcacca ccccaatcac tcaaaaaaca
     1381 aacaaaaata agaagcgaga ggagttttgg cacagcactt tgtgtttaat tgatggcgta
     1441 aaccgcttgg agcttcgtca cgaaaccgct gacaaaatgc aactgaaggc ggacattgac
     1501 gctacgtaac gctacaaacg gtggcgaaag agatagcgga cgcagcggcg aaagagacgg
     1561 cgatatttct gtggacagag aaggaggcaa acagcgctga ctttgagtgg aatgtcattt
     1621 tgagtgagag gtaatcgaaa gaacctggta catcaaatac ccttggatcg aagtaaattt
     1681 aaaactgatc agataagttc aatgatatcc agtgcagtaa aaaaaaaaaa tgtttttttt
     1741 atctactttc cgcaaaaatg ggttttatta acttacatac atactagaat tcggtacccg
     1801 gggatctcga ggtcgacggt atcgataagc ttcgaccgtt ttaacctcga aatatgcaca
     1861 tgtaaggacg gatgtgagcg aacgccagtg atgaccggga tcagaggtaa cctaccatgg
     1921 tggggattag gtgaccgttc gcaggtagtt tgatcggagc gaatgttcgg ggggtctggc
     1981 gtcagaggct ctaaacttta tgtaattcct gccgcgaaac acgcacgtat caagcagtca
     2041 gctgttctct tcgttcagcg cgcgccggtg ttgcaaaacg agcgctcttc gccggcggtg
     2101 gctcgtgcga tagttcgttt tgtcggtaat ccgatgttgc cgcgccgata tcatgtgatg
     2161 ttgtcacagt gcgcgaaatt cgaatggtgg tgtgcagtga ttgtgttgtg acggcgagtg
     2221 gcgcgtgtgg gtgcttagtt ttgggagatg ttttcgtatt tttttgttga taactcaggc
     2281 tttgttgctg tgttgtagta ctattttcca ttgcgcggtg tccagctttt aattagtggc
     2341 acatattctt agcaagtaaa aattattttg catactatta aatttcttat aaattatttt
     2401 ctaaaattaa gtttaccttt tcaattttac taaaaatatc gatatattta ttatcgctgg
     2461 aaaactacat tattccacct ctaagcaaga accgttagtt ggcgcgtagc tttaccacaa
     2521 aattcctgga attgccgtac gcttcgcagt tgtttcaagt tgtctaaggg acatacgatt
     2581 ttttttgcct ctgcgtcacg attttaaccc aaaagcgagt ttagttacat gtacattatt
     2641 attagataaa gaagtatcgc gaatacttca gttgaataaa ctgtgcttgg tttttgggtg
     2701 aggatttgtg gaaagtagag tgcgcgataa ccgtaacttt cgacccggat tttccataga
     2761 tcatgcatca ccatgctagc ctccaaaacc atcgttcttt cggtcggcga ggctactcgc
     2821 actctgactg agatccagtc caccgcagac cgtcagatct tcgaagagaa ggtcgggcct
     2881 ctggtgggtc ggctgcgcct cacggcttcg ctccgtcaaa acggagccaa gaccgcgtat
     2941 cgcgtcaacc taaaactgga tcaggcggac gtcgttgatt ccggacttcc gaaagtgcgc
     3001 tacactcagg tatggtcgca cgacgtgaca atcgttgcga atagcaccga ggcctcgcgc
     3061 aaatcgttgt acgatttgac caagtccctc gtcgcgacct cgcaggtcga agatcttgtc
     3121 gtcaaccttg tgccgctggg ccgtgcggcc gctgtgagca agggcgagga gctgttcacc
     3181 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     3241 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     3301 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     3361 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     3421 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     3481 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     3541 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     3601 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     3661 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     3721 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     3781 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     3841 actctcggca tggacgagct gtacaagtaa accgcggcac actaagcgtc gcgccacttc
     3901 aacgctcgat gggagcgtca ttggtgggcg gggtaaccgt cgaaatcagt gtttacgctt
     3961 ccaatcgcaa caaaaaattc actgcaacac tgaaaagcat acgaaaacga tgaagattgt
     4021 acgagaaacc ataaagtatt ttatccacaa agacacgtat agcagaaaag ccaagttaac
     4081 tcggcgataa gttgtgtaca caagaataaa atcggccaga ttcagtgttg tcagaaataa
     4141 gaaaacccca ctatgttttt ctttgccttt tctttctccc agcgatcatt catttcgtgg
     4201 tgaaagaacg gggtcattgc acggagtttc gactgcggga aagcagagct gccgttcact
     4261 tcgtctataa ttagcgcttt ctattttccc cgattcgggc cgctgctgcg cttttccgcc
     4321 tgctgtttgt ggcaagtgta gcagcaggct gtgcacgcag tgtggcatgc acttggcttt
     4381 ccaccgttgg tatcgattct ctgggacgat gagtcattcc tttcggggcc acagcataat
     4441 cgttgccagc tcaccgaaat ggtgacttca tttcttaact gccgtcaagc atgcgattgt
     4501 acatacatac atatttatat atgtacatat ttatgtgact atggtaggtc gatataatag
     4561 caatcaacgc aagcaaatgt gtcagtcctg cttacaggaa cgattctatt tagtaatttt
     4621 cgttgtataa agtaattatg tatgtatgta agccccataa atctgaaaca attaggcaaa
     4681 accatgcgaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     4741 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     4801 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcaactc
     4861 gaggcctcga ggtcgacctg cagccaagct ttgcgtactc gcaaattatt aaaaataaaa
     4921 ctttaaaaat aatttcgtct aattaatatt atgagttaat tcaaacccca cggacatgct
     4981 aagggttaat caacaatcat atcgctgtct cactcagact caatacgaca ctcagaatac
     5041 tattcctttc actcgcactt attgcaagca tacgttaagt ggatgtctct tgccgacggg
     5101 accaccttat gttatttcat catggtctgg ccattctcat cgtgagcttc cgggtgctcg
     5161 catatctggc tctaagactt cgggcccgac gcaaggagta gccgacatat atccgaaata
     5221 actgcttgtt tttttttttt accattatta ccatcgtgtt tactgtttat tgccccctca
     5281 aaaagctaat gtaattatat ttgtgccaat aaaaacaaga tatgacctat agaatacaag
     5341 tatttcccct tcgaacatcc ccacaagtag actttggatt tgtcttctaa ccaaaagact
     5401 tacacacctg cataccttac atcaaaaact cgtttatcgc tacataaaac accgggatat
     5461 attttttata tacatacttt tcaaatcgcg cgccctcttc ataattcacc tccaccacac
     5521 cacgtttcgt agttgctctt tcgctgtctc ccacccgctc tccgcaacac attcaccttt
     5581 tgttcgacga ccttggagcg actgtcgtta gttccgcgcg attcggtgcg gtatttcaca
     5641 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     5701 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     5761 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     5821 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     5881 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     5941 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     6001 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     6061 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     6121 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     6181 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     6241 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     6301 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     6361 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     6421 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     6481 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     6541 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     6601 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     6661 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     6721 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     6781 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     6841 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     6901 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     6961 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     7021 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     7081 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     7141 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     7201 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     7261 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     7321 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     7381 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     7441 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     7501 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     7561 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     7621 atgctcgtca ggggggcgga gcctatggaa aaacgccttc ttcttgaact cgggctcggt
     7681 gccagtatac ctcaaatggt tgtcgtacct ctcatggttc cgttacgcca acgagggtct
     7741 gctgattaac caatgggcgg acgtggagcc gggcgaaatt agctgcacat cgtcgaacac
     7801 cacgtgcccc agttcgggca aggtcatcct ggagacgctt aacttctccg ccgccgatct
     7861 gccgctggac tacgtgggtc tggcccatga tgaaataaca taaggtggtc ccgtcgatag
     7921 ccgaagctta ccgaagtata cacttaaatt cagtgcacgt ttgcttgttg agaggaaagg
     7981 ttgtgtgcgg acgaattttt ttttgaaaac attaaccctt acgtggaata aaaaaaaatg
     8041 aaatattgca aattttgctg caaagctgtg actggagtaa aattaattca cgtgccgaag
     8101 tgtgctatta agagaaaatt gtgggagcag agccttgggt gcagccttgg tgaaaactcc
     8161 caaatttgtg atacccactt taatgattcg cagtggaagg ctgcacctgc aaaaggtcag
     8221 acatttaaaa ggaggcgact caacgcagat gccgtaccta gtaaagtgat agagcctgaa
     8281 ccagaaaaga taaaagaagg ctataccagt gggagtacac aaacagagta agtttgaata
     8341 gtaaaaaaaa tcatttatgt aaacaataac gtgactgtgc gttaggtcct gttcattgtt
     8401 taatgaaaat aagagcttga gggaaaaaat tcgtactttg gagtacgaaa tgcgtcgttt
     8461 agagcagcag ccgaattaat tctagttcca gtgaaatcca agcattttct aaattaaatg
     8521 tattcttatt attatagttg ttatttttga tatatataaa caacactatt atgcccacca
     8581 tttttttgag atgcatctac acaaggaaca aacactggat gtcactttca gttcaaattg
     8641 taacgctaat cactccgaac aggtcacaaa aaattacctt aaaaagtcat aatattaaat
     8701 tagaataaat atagctgtga gggaaatata tacaaatata ttggagcaaa taaattgtac
     8761 atacaaatat ttattactaa tttctattga gacgaaatga accactcgga accatttgag
     8821 cgaaccgaat cgcgcggaac taacgacagt cgctccaagg tcgtcgaaca aaaggtgaat
     8881 gtgttgcgga gagcgggtgg gagacagcga aagagcaact acgaaacgtg gtgtggtgga
     8941 ggtgaattat gaagagggcg cgcgatttga aaagtatgta tataaaaaat atatcccggt
     9001 gttttatgta gcgataaacg agtttttgat gtaaggtatg caggtgtgta agtcttttgg
     9061 ttagaagaca aatccaaagt ctacttgtgg ggatgttcga aggggaaata cttgtattct
     9121 ataggtcata tcttgttttt attggcacaa atataattac attagctttt tgagggggca
     9181 ataaacagta aacacgatgg taataatggt aaaaaaaaaa aacaagcagt tatttcggat
     9241 atatgtcggc tactccttgc gtcgggcccg aagtcttaga gccagatatg cgagcacccg
     9301 gaagctcacg atgagaatgg ccagacccac gtagtccagc ggcagatcgg cggcggagaa
     9361 gttaagcgtc tccaggatga ccttgcccga actggggcac gtggtgttcg acgatgtgca
     9421 gctaatttcg cccggctcca cgtccgccca ttggttaatc agcagaccct cgttggcgta
     9481 acggaaccat gagaggtacg acaaccattt gaggtatact ggcaccgagc ccgagttcaa
     9541 gaagaagccg ccaaagagca ggaatggtat gataaccggc ggacccacag acagcgccat
     9601 cgaggtcgag gagctggcgc aggatattag atatccgaag gacgttgaca cattggccac
     9661 cagagtgacc agcgccaggc agttgaagaa gtgcagcact ccggcccgca gtccgatcat
     9721 cggataggca atcgccgtga agaccagtgg cactgtgaga aaaagcggca attcggcaat
     9781 cgttttgccc agaaagtatg tgtcacagcg ataaagtcga cttcgggcct ccctcataaa
     9841 aactggcagc tctgaggtga acacctaaat cgaatcgatt cattagaaag ttagtaaatt
     9901 attgaaatgc aaatgtattc taaacatgac ttacatttat cgtggcaaag acgttttgaa
     9961 aggtcatgtt ggtcaggaag aggaagatgg ctccgttgat attcatcaca cccacttgcg
    10021 tgagttgttg gcccaaaaag atgaggccaa tcaagatggc aaccatctgc aaattaaaat
    10081 gttactcgca tctcattaat attcgcgagt taaatgaaat ttatttatct tctgcaaaac
    10141 tataaactat acatctcatt gaaaaaaact aagaagggtg tggaatcagg caattctatc
    10201 taaaatctag cgaatttgtt tccaagaatt gtaagcgtta tatcatttgt ttccactgga
    10261 accactcacc gttgtctgaa taagtcgcac ttttacgagg agtggttcct tgagcaccga
    10321 cagccaggat cgccacagga ccgcccggaa ctgcatgaac caggtggcct tgtaggtgta
    10381 cccattctcc ggctgctcca gtggcttctc cagatttttg gtggccaaca actgctccat
    10441 atcccgggct actttgctaa tggcaaaatt gtcgcatatc ttggcgatcc gatcacggga
    10501 ctcgatctcc cgtccgggca caacggccaa cacctgtacg taaaagtccg ccggattgta
    10561 gttggtagga cactgggcac ccacgctgga taggagttga gatgttatgt aatactagat
    10621 acccttaata aacacatcga actcactagg aaaagaagtc gacggcttcg ctgggagtgc
    10681 ccaagaaagc taccctgccc tcggccatca gaaggatctt gtcaaagagc tcaaacagct
    10741 cggaagacgg ctgatgaatg gtcaggatga cggtcttgcc cttctgcgac agcttcttca
    10801 gcacctggac gacg
//
