LOCUS       sequence-358119-         457 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Doxycycline inducible lentiviral vector for N-terminal Flag tagged 
            KBTBD4 R313PRR expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Chen Z, Ioris RM, Richardson S, Van Ess AN, Vendrell I, Kessler BM, 
            Buffa FM, Busino L, Clifford SC, Bullock AN, D'Angiolella V
  TITLE     Disease-associated KBTBD4 mutations in medulloblastoma elicit 
            neomorphic ubiquitylation activity to promote CoREST degradation.
  JOURNAL   Cell Death Differ. 2022 Apr 4. pii: 10.1038/s41418-022-00983-4. doi:
            10.1038/s41418-022-00983-4.
  PUBMED    35379950
REFERENCE   2  (bases 1 to 457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Death 
            Differ. 2022 Apr 4. pii: 10.1038/s41418-022-00983-4. doi: 
            10.1038/s41418-022-00983-4."
COMMENT     Sequence Label: pCW57.1 KBTBD4 R313PRR
FEATURES             Location/Qualifiers
     source          1..457
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(7..24)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tccttagtgg tggtggtggt ggtggaccgg ttcattacta aaccactttg tacnnknaan
       61 ctgnnwctag atatctcgag tgcggccgcg aattcttagg ccaacacaaa ctgcaaattg
      121 gtaagcagca ccttaatacc tcttgtgaca gttacggctg aagtggcagg gtcaagcttg
      181 taggtgttgg catccccctt tttatatcgg tcccggaaga catagatgct cccgttacag
      241 ctggcaatct tccagaggga tggggcagag ctccaggcct caggaaggca aagccgggtg
      301 aagctgtcta gcaagggatt gtagcacacc agggagtccc cttcagccac gatgaacacc
      361 agatctttat gcacagctgc gtgcatgcgg cctgcaaagg gcagcacata gggcttcaca
      421 tggcatttgt ctgtctctgt gtcaaagcac tggatga
//
