LOCUS       sequence-358127-        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector with N-terminal Flag for KBTBD4 R312RG 
            expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Chen Z, Ioris RM, Richardson S, Van Ess AN, Vendrell I, Kessler BM, 
            Buffa FM, Busino L, Clifford SC, Bullock AN, D'Angiolella V
  TITLE     Disease-associated KBTBD4 mutations in medulloblastoma elicit 
            neomorphic ubiquitylation activity to promote CoREST degradation.
  JOURNAL   Cell Death Differ. 2022 Apr 4. pii: 10.1038/s41418-022-00983-4. doi:
            10.1038/s41418-022-00983-4.
  PUBMED    35379950
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Death 
            Differ. 2022 Apr 4. pii: 10.1038/s41418-022-00983-4. doi: 
            10.1038/s41418-022-00983-4."
COMMENT     Sequence Label: pcDNA 3.1 KBTBD4 R312RG
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(845..863)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(846..863)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(883..900)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    889..1000
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 tcgtacccac tcgttggctg tgtccttgca ctgtgcagaa gatgactcca tcagtgtaag
       61 tggccaaaac agtttgtgcc accagatcac tgcggcctgc aagcatggtg gagacttgta
      121 tgtggtggga gggtccatcc cacggggacg catgtggaag tgcaacaatg ccaccgttga
      181 ctgggagtgg tgtgctcctt tgcctcggga ccggctccag cacaccctgg tgtctgtgcc
      241 cgggaaagat gccatatatt cactgggtgg caagacactg caagataccc tctccaacgc
      301 agtcatttat tatcgcgtag gtgataatgt gtggacagag acaactcagc tagaggtggc
      361 tgtgtcaggg gctgctggtg ccaacctcaa cgggatcatc tacttactag ggggggagga
      421 gaatgatctg gacttcttta ccaaaccttc ccgactcatc cagtgctttg acacagagac
      481 agacaaatgc catgtgaagc cctatgtgct gccctttgca ggccgcatgc acgcagctgt
      541 gcataaagat ctggtgttca tcgtggctga aggggactcc ctggtgtgct acaatccctt
      601 gctagacagc ttcacccggc tttgccttcc tgaggcctgg agctctgccc catccctctg
      661 gaagattgcc agctgtaacg ggagcatcta tgtcttccgg gaccgatata aaaaggggga
      721 tgccaacacc tacaagcttg accctgccac ttcagccgta actgtcacaa gaggtattaa
      781 ggtgctgctt accaatttgc agtttgtgtw ggcctaagtc gagcatgcat ctagagggcc
      841 ctattctata gtgtcaccta aatgctagag ctcgctgatc agcctcgact gtgccttcta
      901 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
      961 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     1021 attctattct gggg
//
