LOCUS       sequence-358158-        8566 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Wild-type LbCas12a (Chen et al., 2018) in a plasmid containing a 
            SC101 origin and lac promoter for in vivo interference assay..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8566)
  AUTHORS   Huang CJ, Adler BA, Doudna JA
  TITLE     A naturally DNase-free CRISPR-Cas12c enzyme silences gene 
            expression.
  JOURNAL   Mol Cell. 2022 Jun 2;82(11):2148-2160.e4. doi: 
            10.1016/j.molcel.2022.04.020.
  PUBMED    35659325
REFERENCE   2  (bases 1 to 8566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2022.04"; journalName: "Mol Cell"; date: 
            "2022-06-2- 2"; volume: "82"; issue: "11"; pages: "2148-2160.e4"
COMMENT     Sequence Label: pCJH027_SC101_pLAC_LbCas12a_Kan
FEATURES             Location/Qualifiers
     source          1..8566
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(664..1614)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      1662..1884
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             complement(2428..3243)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2428..3243)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3151..3170
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3235..3254
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             complement(3428..4510)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4472..4491
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4511..4588)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     4794..4813
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     protein_bind    4881..4897
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    4904..4920
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             join(4961..8566,1..78)
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
ORIGIN
        1 gatgaaaagt tggataaggt caaaattgcg atctcgaata aagaatggct ggaatatgcg
       61 cagaccagcg tgaaacatta agctgcaagg aggactttgg caggtgctct cctgagtagg
      121 acaaatccgc cgccctagac ctagggtacg ggttttgctg cccgcaaacg ggctgttctg
      181 gtgttgctag tttgttatca gaatcgcaga tccggcttca gccggtttgc cggctgaaag
      241 cactatttct tccagaattg ccatgatttt ttccccacgg gaggcgtcac tggctcccgt
      301 gttgtcggca gctttgattc gataagcagc atcgcctgtt tcaggctgtc tatgtgtgac
      361 tgttgagctg taacaagttg tctcaggtgt tcaatttcat gttctagttg ctttgtttta
      421 ctggtttcac ctgttctatt aggtgttaca tgctgttcat ctgttacatt gtcgatctgt
      481 tcatggtgaa cagctttgaa tgcaccaaaa actcgtaaaa gctctgatgt atctatcttt
      541 tttacaccgt tttcatctgt gcatatggac agttttccct ttgatatgta acggtgaaca
      601 gttgttctac ttttgtttgt tagtcttgat gcttcactga tagatacaag agccataaga
      661 acctcagatc cttccgtatt tagccagtat gttctctagt gtggttcgtt gtttttgcgt
      721 gagccatgag aacgaaccat tgagatcata cttactttgc atgtcactca aaaattttgc
      781 ctcaaaactg gtgagctgaa tttttgcagt taaagcatcg tgtagtgttt ttcttagtcc
      841 gttatgtagg taggaatctg atgtaatggt tgttggtatt ttgtcaccat tcatttttat
      901 ctggttgttc tcaagttcgg ttacgagatc catttgtcta tctagttcaa cttggaaaat
      961 caacgtatca gtcgggcggc ctcgcttatc aaccaccaat ttcatattgc tgtaagtgtt
     1021 taaatcttta cttattggtt tcaaaaccca ttggttaagc cttttaaact catggtagtt
     1081 attttcaagc attaacatga acttaaattc atcaaggcta atctctatat ttgccttgtg
     1141 agttttcttt tgtgttagtt cttttaataa ccactcataa atcctcatag agtatttgtt
     1201 ttcaaaagac ttaacatgtt ccagattata ttttatgaat ttttttaact ggaaaagata
     1261 aggcaatatc tcttcactaa aaactaattc taatttttcg cttgagaact tggcatagtt
     1321 tgtccactgg aaaatctcaa agcctttaac caaaggattc ctgatttcca cagttctcgt
     1381 catcagctct ctggttgctt tagctaatac accataagca ttttccctac tgatgttcat
     1441 catctgagcg tattggttat aagtgaacga taccgtccgt tctttccttg tagggttttc
     1501 aatcgtgggg ttgagtagtg ccacacagca taaaattagc ttggtttcat gctccgttaa
     1561 gtcatagcga ctaatcgcta gttcatttgc tttgaaaaca actaattcag acatacatct
     1621 caattggtct aggtgatttt aatcactata ccaattgaga tgggctagtc aatgataatt
     1681 actagtcctt ttcctttgag ttgtgggtat ctgtaaattc tgctagacct ttgctggaaa
     1741 acttgtaaat tctgctatac cctctgtaaa ttccgctaga cctttgtgtg ttttttttgt
     1801 ttatattcaa gtggttataa tttatagaat aaagaaagaa taaaaaaaga taaaaagaat
     1861 agatcccagc cctgtgtata actcactact ttagtcagtt ccgcagtatt acaaaaggat
     1921 gtcgcaaacg ctgtttgctc ctctacaaaa cagaccttaa aaccctaaag gcttaagtag
     1981 caccctcgca agctcgggca aatcgctgaa tattcctttt gtctccgacc atcaggcacc
     2041 tgagtcgctg tctttttcgt gacattcagt tcgctgcgct cacggctctg gcagtgaatg
     2101 ggggtaaatg gcactacagg cgccttttat ggattcatgc aaggaaacta cccataatac
     2161 aagaaaagcc cgtcacgggc ttctcagggc gttttatggc gggtctgcta tgtggtgcta
     2221 tctgactttt tgctgttcag cagttcctgc cctctgattt tccagtctga ccacttcgga
     2281 ttatcccgtg acaggtcatt cagactggct aatgcaccca gtaaggcagc ggtatcatca
     2341 acaggcttac ccgtcttact gtccctagtg tcagatttct tactttattc aggcgattac
     2401 gtgttacaac caattaacca attctgatta gaaaaactca tcgagcatca aatgaaactg
     2461 caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga
     2521 aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat
     2581 tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc
     2641 aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa gcttatgcat
     2701 ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc
     2761 aaccaaaccg ttattcattc gtgattgcgc ctgagcgagg cgaaatacgc gatcgctgtt
     2821 aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc
     2881 aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg ttttcccggg
     2941 gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg
     3001 aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc
     3061 aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg
     3121 atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc
     3181 agcatccatg ttggaattta atcgcggcct ggagcaagac gtttcccgtt gaatatggct
     3241 cataacaccc cttgtattac tgtttatgta agcagacagt tttattgttc atgatgatat
     3301 atttttatct tgtgcaatgt aacatcagag attttgagac acaacgtggc tttgtcgaat
     3361 aaatcgaact tttgctgagt tgaaggatca gcacctgccc gaaccagaga cttatctcag
     3421 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     3481 cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca
     3541 ccagtgatac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca
     3601 agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg
     3661 ggatataaca tgagctgtcc tcggtatcgt cgtatcccac taccgagatg tccgcaccaa
     3721 cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa
     3781 ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg
     3841 acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat
     3901 atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca
     3961 gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtcct
     4021 catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg
     4081 gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa
     4141 tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga
     4201 cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt
     4261 taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa
     4321 tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct
     4381 ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca
     4441 ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta
     4501 ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc
     4561 gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc
     4621 tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat
     4681 ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc
     4741 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg
     4801 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt
     4861 ccggcgtaga ggatcgagaa ttgtgagcgg ataacaattg acattgtgag cggataacaa
     4921 gatactgagc accgtgttaa ctttaagaag gagatatacc atgagcaaac tggagaagtt
     4981 cacgaactgt tattctctgt cgaaaacctt acgttttaag gccattccgg taggtaaaac
     5041 gcaagaaaat atcgacaata aacgtctcct ggttgaagat gaaaagcgtg ccgaagatta
     5101 taaaggtgtt aaaaagctgc ttgatcgcta ttacctgtct tttatcaacg atgttctgca
     5161 ttccatcaaa ttgaaaaact tgaataatta catctcgtta tttcgcaaaa aaacccgtac
     5221 cgaaaaagaa aacaaggagc tggaaaacct tgaaattaat ctgcgtaaag aaatcgcgaa
     5281 agcatttaaa ggtaatgaag gatataaatc tctgtttaaa aaagacatta ttgaaaccat
     5341 cctgcctgag ttcttggatg ataaagatga aattgccctg gttaatagct ttaatggctt
     5401 tacgactgca tttaccggct tttttgataa ccgtgaaaac atgttcagcg aagaagcgaa
     5461 atcgacttcc attgccttcc gttgcattaa tgaaaacctg acccgctaca tttctaatat
     5521 ggacatcttt gaaaaagttg atgccatttt cgacaaacat gaagttcaag aaatcaaaga
     5581 aaagatttta aactccgatt atgatgttga agatttcttt gagggagaat ttttcaattt
     5641 tgttctcacc caagagggta ttgatgtcta taacgccatt atcggaggat ttgttacgga
     5701 atcaggcgaa aagattaaag gactgaatga atacattaat ttatacaatc agaaaaccaa
     5761 acagaaattg ccaaaattca aaccactgta taaacaggta ttaagcgacc gtgaatcctt
     5821 gtccttttat ggcgaaggct atacaagcga tgaagaggtg ctcgaagtat tccgtaatac
     5881 tttgaacaaa aactcggaaa tcttctccag cattaaaaaa ctggaaaaat tgtttaaaaa
     5941 tttcgatgaa tatagtagcg caggcatctt cgtcaaaaat ggccctgcga tttcaaccat
     6001 ttctaaagac attttcggtg aatggaacgt gatccgcgat aaatggaacg cggagtatga
     6061 cgacattcat ctgaaaaaaa aagccgttgt taccgaaaag tatgaagatg accgccgcaa
     6121 gagctttaaa aaaattggta gtttctcact ggaacagtta caagaatatg cagacgccga
     6181 tctgtcggtt gtggaaaagt taaaagagat tattatccag aaagtcgatg aaatttataa
     6241 agtgtacggc tccagcgaaa agctctttga tgccgacttt gttttagaaa aatcgctgaa
     6301 aaaaaacgat gccgtggtgg ccatcatgaa agatctgttg gattcggtca aatcgttcga
     6361 aaactatatc aaggcgttct ttggcgaagg gaaagagacc aatcgtgacg aatcgttcta
     6421 tggcgatttt gtgttggcgt atgatattct tctgaaagta gatcacatct atgatgctat
     6481 ccgcaactac gtgacccaga aaccgtattc gaaggataaa tttaagctgt attttcaaaa
     6541 ccctcagttt atgggtggct gggataaaga taaagaaacc gactaccgcg cgacgatctt
     6601 gcgctatggc tctaagtact atttagccat catggataaa aagtatgcaa aatgcctcca
     6661 gaaaatcgat aaggatgatg tcaacgggaa ctacgaaaaa atcaactaca aactcttgcc
     6721 tggcccgaat aaaatgctgc cgaaagtctt tttttccaaa aaatggatgg cctattataa
     6781 cccttccgaa gatattcaga agatttacaa gaacggtacc tttaaaaaag gtgacatgtt
     6841 taatctgaat gactgtcaca agcttattga tttcttcaaa gatagtattt ctcgctaccc
     6901 taaatggtca aacgcgtacg attttaattt ctcagaaaca gagaaataca aagatattgc
     6961 ggggttttat cgcgaagtcg aagaacaagg gtataaggtg tcgttcgaat cggcttccaa
     7021 aaaggaagtg gataaacttg ttgaagaagg caaactctat atgttccaga tctacaacaa
     7081 agatttctcc gacaagagcc atggcacgcc gaacttacat accatgtact ttaaattact
     7141 gttcgatgaa aataatcatg gtcagattcg cttgagcggt ggcgccgaac tgtttatgcg
     7201 ccgcgcatca ttgaaaaagg aagaacttgt tgtgcaccca gcaaacagtc cgattgcaaa
     7261 caaaaatccg gataacccga agaagactac caccttaagc tatgatgtat ataaagataa
     7321 acgttttagc gaagatcagt atgaactcca cattccgatc gcgattaaca aatgtcctaa
     7381 gaacatcttt aaaatcaata ccgaggttcg tgtactgctc aaacacgacg acaaccccta
     7441 tgttattggc attgaccgcg gcgaacgcaa cctgctgtat attgtcgttg tggatggcaa
     7501 aggtaacatt gttgaacagt atagccttaa tgaaatcatc aataatttca acggcattcg
     7561 tattaaaacg gactatcata gcctgctgga caaaaaagag aaagaacgct tcgaagcccg
     7621 tcagaattgg acctctatcg aaaatatcaa ggaactgaaa gctgggtaca tttctcaagt
     7681 ggtccacaag atttgcgaac tggtggaaaa gtatgacgcg gtgatcgcac tggaggatct
     7741 gaattcgggc tttaaaaata gccgtgtgaa ggtggagaaa caggtttacc aaaagttcga
     7801 aaaaatgctg attgacaaac ttaattacat ggtggacaaa aaatctaatc catgtgcgac
     7861 cggcggggct ctcaaaggct atcagattac gaataaattc gaaagcttca aatccatgtc
     7921 tacccagaac ggatttatct tttacatccc cgcctggctg acttccaaaa ttgatccgag
     7981 taccggtttt gttaatctgc tgaaaaccaa atatacgtcg atcgccgatt caaaaaaatt
     8041 cattagcagc ttcgaccgta ttatgtacgt cccggaagaa gacctgttcg aattcgccct
     8101 tgactataaa aatttctcgc gtacggatgc cgattatatc aaaaaatgga aactgtacag
     8161 ctacggcaac cgcatccgca tttttcgcaa cccaaagaag aacaacgtat ttgactggga
     8221 ggaagtttgc ttgacgagcg cttacaaaga gctctttaac aaatatggga tcaattatca
     8281 gcaaggggac atccgtgcgt tgctgtgtga gcagtcagat aaagcgttct acagctcttt
     8341 catggcttta atgtcgctga tgttgcagat gcgcaattcg atcactggcc gcaccgatgt
     8401 ggatttcctg attagcccgg tcaaaaatag cgatggcatt ttttacgatt cacgtaacta
     8461 tgaagcccag gaaaacgcta ttctgccgaa gaacgctgat gcgaacggcg cgtacaatat
     8521 tgcccgtaag gtactgtggg caattggtca gttcaagaaa gcggaa
//
