LOCUS       sequence-358179-        9767 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  to make movies of LRP6 vesicles.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9767)
  AUTHORS   Colozza G, Jami-Alahmadi Y, Dsouza A, Tejeda-Munoz N, Albrecht LV, 
            Sosa EA, Wohlschlegel JA, De Robertis EM
  TITLE     Wnt-inducible Lrp6-APEX2 interacting proteins identify ESCRT 
            machinery and Trk-fused gene as components of the Wnt signaling 
            pathway.
  JOURNAL   Sci Rep. 2020 Dec 9;10(1):21555. doi: 10.1038/s41598-020-78019-5.
  PUBMED    33299006
REFERENCE   2  (bases 1 to 9767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2020-12-9"; volume: "10(1):21555. doi"; pages: " 
            10.1038/s41598-020-78019-5"
COMMENT     Sequence Label: pCS2 LRP6-eGFP
FEATURES             Location/Qualifiers
     source          1..9767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        325..343
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     325..342
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    398..422
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             498..530
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     protein_bind    complement(5320..5344)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(5352..5354,5355..5357,5358..6071)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5352..5354,5355..5357,5358..6068)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5397..5418)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5658..5677)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6005..6026
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6109..6243
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6133..6152)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6187..6206
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(6356..6376)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6356..6374)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6395..6411)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6395..6411)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6408..6430)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6419..6435
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6443..6449,6450..6467,6468..6473))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6488..6509
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6626..6643)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6797..7385)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6877..6896)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7556..8347,8348..8416))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8179..8198
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8417..8521)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8547..9002)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8684..8705)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8896..8915
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        join(9073..9767,1..290)
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 gttctcacgc cccctattga cgtcaatgac ggtaaatggc ccacttggca gtacatcaat
       61 atctattaat agtaacttgg caagtacatt actattggaa gtacgccagg gtacattggc
      121 agtactccca ttgacgtcaa tggcggtaaa tggcccgcga tggctgccaa gtacatcccc
      181 attgacgtca atggggaggg gcaatgacgc aaatgggcgt tccattgacg taaatgggcg
      241 gtaggcgtgc ctaatgggag gtctatataa gcaatgctcg tttagggaac cgccattctg
      301 cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag ctacttgttc
      361 tttttgcagg atcccatcga ttcgaattca aggatcaaca agtttgtaca aaaaagcagg
      421 ctaccatggg ggccgtcctg aggagcctcc tggcctgcag cttctgtgtg ctcctgagag
      481 cggccccttt gttgctttat actgatatcg aaatgaaccg cctgggtaag gctccggaac
      541 ctttgttgct ttatgcaaac agacgggact tgcgattggt tgatgctaca aatggcaaag
      601 agaatgctac gattgtagtt ggaggcttgg aggatgcagc tgcggtggac tttgtgttta
      661 gtcatggctt gatatactgg agtgatgtca gcgaagaagc cattaaacga acagaattta
      721 acaaaactga gagtgtgcag aatgttgttg tttctggatt attgtccccc gatgggctgg
      781 catgtgattg gcttggagaa aaattgtact ggacagattc tgaaactaat cggattgaag
      841 tttctaattt agatggatct ttacgaaaag ttttattttg gcaagagttg gatcaaccca
      901 gagctattgc cttagatcct tcaagtgggt tcatgtactg gacagactgg ggagaagtgc
      961 caaagataga acgtgctgga atggatggtt caagtcgctt cattataata aacagtgaaa
     1021 tttactggcc aaatggactg actttggatt atgaagaaca aaagctttat tgggcagatg
     1081 caaaacttaa tttcatccac aaatcaaatc tggatggaac aaatcggcag gcagtggtta
     1141 aaggttccct tccacatcct tttgccttga cgttatttga ggacatattg tactggactg
     1201 actggagcac acactccatt ttggcttgca acaagtatac tggtgagggt ctgcgtgaaa
     1261 tccattctga catcttctct cccatggata tacatgcctt cagccaacag aggcagccaa
     1321 atgccacaaa tccatgtgga attgacaatg ggggttgttc ccatttgtgt ttgatgtctc
     1381 cagtcaagcc tttttatcag tgtgcttgcc ccactggggt caaactcctg gagaatggaa
     1441 aaacctgcaa agatggtgcc acagaattat tgcttttagc tcgaaggaca gacttgagac
     1501 gcatttcttt ggatacacca gattttacag acattgttct gcagttagaa gacatccgtc
     1561 atgccattgc catagattac gatcctgtgg aaggctacat ctactggact gatgatgaag
     1621 tgagggccat acgccgttca tttatagatg gatctggcag tcagtttgtg gtcactgctc
     1681 aaattgccca tcctgatggt attgctgtgg actgggttgc acgaaatctt tattggacag
     1741 acactggcac tgatcgaata gaagtgacaa ggctcaatgg gaccatgagg aagatcttga
     1801 tttcagagga cttagaggaa ccccgggcta ttgtgttaga tcccatggtt gggtacatgt
     1861 attggactga ctggggagaa attccgaaaa ttgagcgagc agctctggat ggttctgacc
     1921 gtgtagtatt ggttaacact tctcttggtt ggccaaatgg tttagccttg gattatgatg
     1981 aaggcaaaat atactgggga gatgccaaaa cagacaagat tgaggttatg aatactgatg
     2041 gcactgggag acgagtacta gtggaagaca aaattcctca catatttgga tttactttgt
     2101 tgggtgacta tgtttactgg actgactggc agaggcgtag cattgaaaga gttcataaac
     2161 gaagtgcaga gagggaagtg atcatagatc agctgcctga cctcatgggc ctaaaggcta
     2221 caaatgttca tcgagtgatt ggttccaacc cctgtgctga ggaaaacggg ggatgtagcc
     2281 atctctgcct ctatagacct cagggccttc gctgtgcttg ccctattggc tttgaactca
     2341 tcagtgacat gaagacctgc attgtcccag aggctttcct tttgttttca cggagagcag
     2401 atatcagacg aatttctctg gaaacaaaca ataataatgt ggctattcca ctcactggtg
     2461 tcaaagaagc ttctgctttg gattttgatg tgacagacaa ccgaatttat tggactgata
     2521 tatcactcaa gaccatcagc agagccttta tgaatggcag tgcactggaa catgtggtag
     2581 aattcggctt agattatcca gaaggcatgg cagtagactg gcttgggaag aacttgtact
     2641 gggcagacac aggaacgaat cgaattgagg tgtcaaagtt ggatgggcag caccgacaag
     2701 ttttggtgtg gaaagaccta gatagtccca gagctctcgc gttggaccct gccgaaggat
     2761 ttatgtattg gactgaatgg ggtggaaaac ctaagataga cagagctgca atggatggaa
     2821 gtgaacgtac taccttagtt ccaaatgtgg ggcgggcaaa cggcctaact attgattatg
     2881 ctaaaaggag gctttattgg acagacctgg acaccaactt aatagaatct tcaaatatgc
     2941 ttgggctcaa ccgtgaagtt atagcagatg acttgcctca tccttttggc ttaactcagt
     3001 accaagatta tatctactgg acggactgga gccgacgcag cattgagcgt gccaacaaaa
     3061 ccagtggcca aaaccgcacc atcattcagg gccatttgga ttatgtgatg gacatcctcg
     3121 tctttcactc atctcgacag tcagggtgga atgaatgtgc ttccagcaat gggcactgct
     3181 cccacctctg cttggctgtg ccagttgggg gttttgtttg tggatgccct gcccactact
     3241 ctcttaatgc tgacaacagg acttgtagtg ctcctacgac tttcctgctc ttcagtcaaa
     3301 agagtgccat caaccgcatg gtgattgatg aacaacagag ccccgacatc atccttccca
     3361 tccacagcct tcggaatgtc cgggccattg actatgaccc actggacaag caactctatt
     3421 ggattgactc acgacaaaac atgatccgaa aggcacaaga agatggcagc cagggcttta
     3481 ctgtggttgt gagctcagtt ccgagtcaga acctggaaat acaaccctat gacctcagca
     3541 ttgatattta cagccgctac atctactgga cttgtgaggc taccaatgtc attaatgtga
     3601 caagattaga tgggagatca gttggagtgg tgctgaaagg cgagcaggac agacctcgag
     3661 ccattgtggt aaacccagag aaagggtata tgtattttac caatcttcag gaaaggtctc
     3721 ctaaaattga acgggctgct ttggatggga cagaacggga ggtcctcttt ttcagtggct
     3781 taagtaaacc aattgcttta gcccttgata gcaggctggg caagctcttt tgggctgatt
     3841 cagatctccg gcgaattgaa agcagtgatc tctcaggtgc taaccggata gtattagaag
     3901 actccaatat cttgcagcct gtgggactta ctgtgtttga aaactggctc tattggattg
     3961 ataaacagca gcaaatgatt gaaaaaattg acatgacagg tcgagagggt agaaccaaag
     4021 tccaagctcg aattgcccag cttagtgaca ttcatgcagt aaaggagctg aaccttcaag
     4081 aatacagaca gcacccttgt gctcaggata atggtggctg ttcacatatt tgtcttgtaa
     4141 agggggatgg tactacaagg tgttcttgcc ccatgcacct ggttctactt caagatgagc
     4201 tatcatgtgg agaacctcca acatgttctc ctcagcagtt tacttgtttc acgggggaaa
     4261 ttgactgtat ccctgtggct tggcggtgcg atgggtttac tgaatgtgaa gaccacagtg
     4321 atgaactcaa ttgtcctgta tgctcagagt cccagttcca gtgtgccagt gggcagtgta
     4381 ttgatggtgc cctccgatgc aatggagatg caaactgcca ggacaaatca gatgagaaga
     4441 actgtgaagt gctttgttta attgatcagt tccgctgtgc caatggtcag tgcattggaa
     4501 agcacaagaa gtgtgatcat aatgtggatt gcagtgacaa gtcagatgaa ctggattgtt
     4561 atccgactga agaaccagca ccacaggcca ccaatacagt tggttctgtt attggcgtaa
     4621 ttgtcaccat ttttgtgtct ggaactgtat actttatctg ccagaggatg ttgtgtccac
     4681 gtatgaaggg agatggggaa actatgacta atgactatgt agttcatgga ccagcttctg
     4741 tgcctcttgg ttatgtgcca cacccaagtt ctttgtcagg atctcttcca ggaatgtctc
     4801 gaggtaaatc aatgatcagc tccctcagta tcatgggggg aagcagtgga cccccctatg
     4861 accgagccca tgttacagga gcatcatcaa gtagttcttc aagcaccaaa ggcacttact
     4921 tccctgcaat tttgaaccct ccaccatccc cagccacaga gcgatcacat tacactatgg
     4981 aatttggata ttcttcaaac agtccttcca ctcataggtc atacagctac aggccatata
     5041 gctaccggca ctttgcaccc cccaccacac cctgcagcac agatgtttgt gacagtgact
     5101 atgctcctag tcggagaatg acctcagtgg caacagccaa gggctatacc agtgacttga
     5161 actatgattc agaacctgtg cccccacctc ccacaccccg aagccaatac ttgtcagcag
     5221 aggagaacta tgaaagctgc ccaccttctc catacacaga gaggagctat tctcatcacc
     5281 tctacccacc gccaccctct ccctgtacag actcctccga cccagctttc ttgtacaaag
     5341 tggttgatcc tatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     5401 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     5461 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     5521 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     5581 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     5641 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     5701 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     5761 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     5821 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     5881 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     5941 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     6001 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     6061 tgtacaagta actcgagcct ctagaactat agtgagtcgt attacgtaga tccagacatg
     6121 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     6181 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     6241 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     6301 ttttaattcg cggccgcggc gccaatgcat tgggcccggt acccagcttt tgttcccttt
     6361 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     6421 gttatccgct cacaattcca cacaacatac gagccgggag cataaagtgt aaagcctggg
     6481 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     6541 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     6601 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     6661 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     6721 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     6781 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     6841 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     6901 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     6961 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     7021 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     7081 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7141 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7201 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     7261 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     7321 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     7381 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     7441 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     7501 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     7561 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     7621 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     7681 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     7741 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     7801 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     7861 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     7921 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     7981 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     8041 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     8101 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     8161 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     8221 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     8281 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     8341 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     8401 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     8461 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     8521 gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc
     8581 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     8641 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     8701 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     8761 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     8821 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     8881 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     8941 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     9001 gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     9061 gctattacgc cagtcgaccg ccaattcaat atggcgtata tggactcatg ccaattcaat
     9121 atggtggatc tggacctgtg ccaattcaat atggcgtata tggactcgtg ccaattcaat
     9181 atggtggatc tggaccccag ccaattcaat atggcggact tggcaccatg ccaattcaat
     9241 atggcggacc tggcactgtg ccaactgggg aggggtctac ttggcacggt gccaagtttg
     9301 aggaggggtc ttggccctgt gccaagtccg ccatattgaa ttggcatggt gccaataatg
     9361 gcggccatat tggctatatg ccaggatcaa tatataggca atatccaata tggccctatg
     9421 ccaatatggc tattggccag gttcaatact atgtattggc cctatgccat atagtattcc
     9481 atatatgggt tttcctattg acgtagatag cccctcccaa tgggcggtcc catataccat
     9541 atatggggct tcctaatacc gcccatagcc actcccccat tgacgtcaat ggtctctata
     9601 tatggtcttt cctattgacg tcatatgggc ggtcctattg acgtatatgg cgcctccccc
     9661 attgacgtca attacggtaa atggcccgcc tggctcaatg cccattgacg tcaataggac
     9721 cacccaccat tgacgtcaat gggatggctc attgcccatt catatcc
//
