LOCUS       sequence-358239-        3768 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing FOXD4L1 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-FOXD4L1
FEATURES             Location/Qualifiers
     source          1..3768
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1325..1424)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1441..1460)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1442..1460)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1583..1602)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1594..2403
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1661..1680)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2550..3138
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3039..3058
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3292..3309
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3468..3495)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3587..3673)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3722..3744
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3736..3753
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3737..3753
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tgaacctgcc cagagccgag
      121 aggcccagaa gcacccccca gaggagcctg agagacagcg acggcgagga cggcaagatc
      181 gacgtgctgg gcgaggagga agacgaggac gaggtggagg acgaagagga ggaggccagc
      241 cagaagttcc tggagcagag cctgcagccc ggcctccagg tggctagatg gggaggcgtg
      301 gccctgccta gagagcatat cgagggcgga ggccccagcg acccttctga gtttggcaca
      361 gagttcaggg ccccacccag atccgctgcc gccagcgagg atgccagaca gcccgccaag
      421 cctccctaca gctacatcgc cctgatcacc atggccatcc tgcagagccc ccacaagagg
      481 ctgaccctga gcggcatctg cgccttcatc agcggcagat tcccctacta caggagaaag
      541 ttccccgcct ggcagaacag catcagacac aacctgagcc tgaacgactg cttcgtgaag
      601 atccccaggg aacccggcca ccccggcaag ggcacctact ggagcctgga ccccgcctcc
      661 caggacatgt tcgacaacgg cagcttcctg agaagaagaa agagattcaa gagacaccag
      721 ctgacacctg gcgcccatct gcctcacccc ttccccttac cagctgccca cgctgccctg
      781 cacaatccta gacctggccc cctgctggga gcccctgccc ttcctcagcc tgtgcctggc
      841 gcttacccta acaccgcccc tggaagaaga ccctatgccc tgctgcaccc ccaccctccc
      901 agatacctgc tgctgagcgc ccctgcttac gccggcgccc ctaagaaagc cgagggcgct
      961 gaccttgcta cccctggcac actgcctgtg ctgcagcctt ctctgggacc ccagccttgg
     1021 gaggaaggca agggactggc cagcccccct ggcggcggat gcatcagctt cagcatcgag
     1081 agcatcatgc agggcgtgag aggagctggc accggcgccg cccagtcact gagccctacc
     1141 gcctggagct actgtcctct gctgcagagg cctagcagcc tgagcgacaa tttcgccgct
     1201 accgccgccg cctctggagg cggactgaga cagagactga gatcacacca gggcagaggc
     1261 gccggaagag ccccagtggg aagagtgggc gctgctgccg tgagcggggg cggaagaggc
     1321 ctgtacccaa ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt
     1381 tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc
     1441 ccctatagtg agtcgtatta catggtcata gctgtttctt ggcagctctg gcccgtgtct
     1501 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg
     1561 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg
     1621 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat
     1681 aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag
     1741 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga
     1801 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct
     1861 gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa
     1921 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg
     1981 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag
     2041 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat
     2101 ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat
     2161 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta
     2221 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
     2281 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
     2341 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
     2401 taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac
     2461 ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca
     2521 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     2581 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     2641 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     2701 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     2761 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     2821 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     2881 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     2941 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     3001 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     3061 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     3121 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     3181 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     3241 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     3301 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     3361 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     3421 gcaattaata cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg
     3481 tcaggatggc cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc
     3541 accctccggg ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca
     3601 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct
     3661 ttcgttttat ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt
     3721 tcccagtcac gacgttgtaa aacgacggcc agtcttaagc tcgggccc
//
