LOCUS       sequence-358352-       11179 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Cas9 and contains guide RNA expression cassette with 
            BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 
            marker for yeast transformation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11179)
  TITLE     Patricia Wittkopp yeast CRISPR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pML104-HygMx4
FEATURES             Location/Qualifiers
     source          1..11179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..667
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     537..560
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     regulatory      696..705
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             702..2420
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     CDS             4815..4835
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      4863..5050
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4918..4942)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     terminator      complement(5168..5187)
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     promoter        complement(5269..5537)
                     /label=SNR52 promoter
                     /note="promoter for the S. cerevisiae small nucleolar RNA 
                     gene SNR52"
     primer_bind     complement(5568..5588)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5568..5586)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5607..5623)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5607..5623)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5620..5642)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5631..5647
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5655..5661,5662..5679,5680..5685))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5700..5721
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5838..5855)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6009..6597)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6089..6108)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6768..7559,7560..7628))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7391..7410
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7629..7733)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7760..9102
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(8723..8770)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     9143..9161
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9199..9221)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9321..9340
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     gene            9529..11104
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     promoter        9529..9872
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             9873..10901
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEQFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      10907..11104
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 tcattatcaa tactgccatt tcaaagaata cgtaaataat taatagtagt gattttccta
       61 actttattta gtcaaaaaat tagcctttta attctgctgt aacccgtaca tgcccaaaat
      121 agggggcggg ttacacagaa tatataacat cgtaggtgtc tgggtgaaca gtttattcct
      181 ggcatccact aaatataatg gagcccgctt tttaagctgg catccagaaa aaaaaagaat
      241 cccagcacca aaatattgtt ttcttcacca accatcagtt cataggtcca ttctcttagc
      301 gcaactacag agaacagggg cacaaacagg caaaaaacgg gcacaacctc aatggagtga
      361 tgcaacctgc ctggagtaaa tgatgacaca aggcaattga cccacgcatg tatctatctc
      421 attttcttac accttctatt accttctgct ctctctgatt tggaaaaagc tgaaaaaaaa
      481 ggttgaaacc agttccctga aattattccc ctacttgact aataagtata taaagacggt
      541 aggtattgat tgtaattctg taaatctatt tcttaaactt cttaaattct acttttatag
      601 ttagtctttt ttttagtttt aaaacaccaa gaacttagtt tcgaataaac acacataaac
      661 aaacaaaaga tctgatatgg atcgaattag atctcgccac catggacaag aagtattcta
      721 tcggactgga catcgggact aatagcgtcg ggtgggccgt catcactgac gagtacaagg
      781 tgccctctaa gaagttcaag gtgctcggga acaccgaccg gcattccatc aagaaaaatc
      841 tgatcggagc tctcctcttt gattcagggg agaccgctga agcaacccgc ctcaagcgga
      901 ctgctagacg gcggtacacc aggaggaaga accggatttg ttaccttcaa gagatattct
      961 ccaacgaaat ggcaaaggtc gacgacagct tcttccatag gctggaagaa tcattcctcg
     1021 tggaagagga taagaagcat gaacggcatc ccatcttcgg taatatcgtc gacgaggtgg
     1081 cctatcacga gaaataccca accatctacc atcttcgcaa aaagctggtg gactcaaccg
     1141 acaaggcaga cctccggctt atctacctgg ccctggccca catgattaag ttcagaggcc
     1201 acttcctgat cgagggcgac ctcaatcctg acaatagcga tgtggataaa ctgttcatcc
     1261 agctggtgca gacttacaac cagctctttg aagagaaccc catcaatgca agcggagtcg
     1321 atgccaaggc cattctgtca gcccggctgt caaagagccg cagacttgag aatcttatcg
     1381 ctcagctgcc gggtgaaaag aaaaatggac tgttcgggaa cctgattgct ctttcacttg
     1441 ggctgactcc caatttcaag tctaatttcg acctggcaga ggatgccaag ctgcaactgt
     1501 ccaaggacac ctatgatgac gatctcgaca acctcctggc ccagatcggt gaccaatacg
     1561 ccgacctttt ccttgctgct aagaatcttt ctgacgccat cctgctgtct gacattctcc
     1621 gcgtgaacac tgaaatcacc aaggcccctc tttcagcttc aatgattaag cggtatgatg
     1681 agcaccacca ggacctgacc ctgcttaagg cactcgtccg gcagcagctt ccggagaagt
     1741 acaaggaaat cttctttgac cagtcaaaga atggatacgc cggctacatc gacggaggtg
     1801 cctcccaaga ggaattttat aagtttatca aacctatcct tgagaagatg gacggcaccg
     1861 aagagctcct cgtgaaactg aatcgggagg atctgctgcg gaagcagcgc actttcgaca
     1921 atgggagcat tccccaccag atccatcttg gggagcttca cgccatcctt cggcgccaag
     1981 aggacttcta cccctttctt aaggacaaca gggagaagat tgagaaaatt ctcactttcc
     2041 gcatccccta ctacgtggga cccctcgcca gaggaaatag ccggtttgct tggatgacca
     2101 gaaagtcaga agaaactatc actccctgga acttcgaaga ggtggtggac aagggagcca
     2161 gcgctcagtc attcatcgaa cggatgacta acttcgataa gaacctcccc aatgagaagg
     2221 tcctgccgaa acattccctg ctctacgagt actttaccgt gtacaacgag ctgaccaagg
     2281 tgaaatatgt caccgaaggg atgaggaagc ccgcattcct gtcaggcgaa caaaagaagg
     2341 caattgtgga ccttctgttc aagaccaata gaaaggtgac cgtgaagcag ctgaaggagg
     2401 actatttcaa gaaaattgaa tgcttcgact ctgtggagat tagcggggtc gaagatcggt
     2461 tcaacgcaag cctgggtacc taccatgatc tgcttaagat catcaaggac aaggattttc
     2521 tggacaatga ggagaaagag gacatccttg aggacattgt cctgactctc actctgttcg
     2581 aggaccggga aatgatcgag gagaggctta agacctacgc ccatctgttc gacgataaag
     2641 tgatgaagca acttaaacgg agaagatata ccggatgggg acgccttagc cgcaaactca
     2701 tcaacggaat ccgggacaaa cagagcggaa agaccattct tgatttcctt aagagcgacg
     2761 gattcgctaa tcgcaacttc atgcaactta tccatgatga ttccctgacc tttaaggagg
     2821 acatccagaa ggcccaagtg tctggacaag gtgactcact gcacgagcat atcgcaaatc
     2881 tggctggttc acccgctatt aagaagggta ttctccagac cgtgaaagtc gtggacgagc
     2941 tggtcaaggt gatgggtcgc cataaaccag agaacattgt catcgagatg gccagggaaa
     3001 accagactac ccagaaggga cagaagaaca gcagggagcg gatgaaaaga attgaggaag
     3061 ggattaagga gctcgggtca cagatcctta aagagcaccc ggtggaaaac acccagcttc
     3121 agaatgagaa gctctatctg tactaccttc aaaatggacg cgatatgtat gtggaccaag
     3181 agcttgatat caacaggctc tcagactacg acgtggacca catcgtccct cagagcttcc
     3241 tcaaagacga ctcaattgac aataaggtgc tgactcgctc agacaagaac cggggaaagt
     3301 cagataacgt gccctcagag gaagtcgtga aaaagatgaa gaactattgg cgccagcttc
     3361 tgaacgcaaa gctaatcact cagcggaagt tcgacaatct cactaaggct gagaggggcg
     3421 gactgagcga actggacaaa gcaggattca ttaaacggca acttgtggag actcggcaga
     3481 ttactaaaca tgtagcccaa atccttgact cacgcatgaa taccaagtac gacgaaaacg
     3541 acaaacttat ccgcgaggtg aaggtgatta ccctgaagtc caagctggtc agcgatttca
     3601 gaaaggactt tcaattctac aaagtgcggg agatcaataa ctatcatcat gctcatgacg
     3661 catatctgaa tgccgtggtg ggaaccgccc taatcaagaa gtacccaaag ctggaaagcg
     3721 agttcgtgta cggagactac aaggtctacg acgtgcgcaa gatgattgcc aaatctgagc
     3781 aggagatcgg aaaggccacc gcaaagtact tcttctacag caacatcatg aatttcttca
     3841 agaccgaaat cacccttgca aacggtgaga tccggaagag gccgctcatc gagactaatg
     3901 gggagactgg cgaaatcgtg tgggacaagg gcagagattt cgctaccgtg cgcaaagtgc
     3961 tttctatgcc tcaagtgaac atcgtgaaga aaaccgaggt gcaaaccgga ggcttttcta
     4021 aggaatcaat cctccccaag cgcaactccg acaagctcat tgcaaggaag aaggattggg
     4081 accctaagaa gtacggcgga ttcgattcac caactgtggc ttattctgtc ctggtcgtgg
     4141 ctaaggtgga aaaaggaaag tctaagaagc tcaagagcgt gaaggaactg ctgggtatca
     4201 ccattatgga gcgcagctcc ttcgagaaga acccaattga ctttctcgaa gccaaaggtt
     4261 acaaggaagt caagaaggac cttatcatca agctcccaaa gtatagcctg ttcgaactgg
     4321 agaatgggcg gaagcggatg ctcgcctccg ctggcgaact tcagaagggt aatgagctgg
     4381 ctctcccctc caagtacgtg aatttcctct accttgcaag ccattacgag aagctgaagg
     4441 ggagccccga ggacaacgag caaaagcaac tgtttgtgga gcagcataag cattatctgg
     4501 acgagatcat tgagcagatt tccgagtttt ctaaacgcgt cattctcgct gatgccaacc
     4561 tcgataaagt ccttagcgca tacaataagc acagagacaa accaattcgg gagcaggctg
     4621 agaatatcat ccacctgttc accctcacca atcttggtgc ccctgccgca ttcaagtact
     4681 tcgacaccac catcgaccgg aaacgctata cctccaccaa agaagtgctg gacgccaccc
     4741 tcatccacca gagcatcacc ggactttacg aaactcggat tgacctctca cagctcggag
     4801 gggatgaggg agctcccaag aaaaagcgca aggtaggtag ttccggatcc tagactctcg
     4861 aggcgaattt cttatgattt atgattttta ttattaaata agttataaaa aaaataagtg
     4921 tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa attcttattc ttgagtaact
     4981 ctttcctgta ggtcaggttg ctttctcagg tatagcatga ggtcgctctt attgaccaca
     5041 cctctaccgg catgccgagc aaatgcctgc aaatcgctcc ccatttctct agagcggccg
     5101 tggtatcgtt tagattggca attacagtgt cttagctcac atgcttataa ctaattacat
     5161 gactcgaaga cataaaaaac aaaaaaagca ccaccgactc ggtgccactt tttcaagttg
     5221 ataacggact agccttattt taacttgcta tttaaatgca tcgattctga tcatttatct
     5281 ttcactgcgg agaagtttcg aacgccgaaa catgcgcacc aactttcact tctacagcgt
     5341 ttgaccaaaa tcttttgaac agaacattgt agggtgtgaa aaaatgcgca cctttaccgc
     5401 tagcccaaga gggcactaca aaatctagag ttgtacttca aacgtacatg taatcacctt
     5461 gtatatactc gaaagaaaac atcaagtttc tgtataaata tgagtgaaag cataatcata
     5521 cattatcttt tcaaagattg tccgcggtgg agctccagct tttgttccct ttagtgaggg
     5581 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     5641 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     5701 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     5761 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     5821 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     5881 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     5941 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     6001 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     6061 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     6121 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     6181 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     6241 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     6301 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     6361 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     6421 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     6481 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     6541 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     6601 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     6661 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     6721 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     6781 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     6841 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     6901 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     6961 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     7021 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     7081 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     7141 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     7201 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     7261 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     7321 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     7381 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     7441 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     7501 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     7561 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     7621 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     7681 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     7741 ccccgaaaag tgccacctga acgaagcatc tgtgcttcat tttgtagaac aaaaatgcaa
     7801 cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg
     7861 caacgcgaaa gcgctatttt accaacgaag aatctgtgct tcatttttgt aaaacaaaaa
     7921 tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag
     7981 aaatgcaacg cgagagcgct attttaccaa caaagaatct atacttcttt tttgttctac
     8041 aaaaatgcat cccgagagcg ctatttttct aacaaagcat cttagattac tttttttctc
     8101 ctttgtgcgc tctataatgc agtctcttga taactttttg cactgtaggt ccgttaaggt
     8161 tagaagaagg ctactttggt gtctattttc tcttccataa aaaaagcctg actccacttc
     8221 ccgcgtttac tgattactag cgaagctgcg ggtgcatttt ttcaagataa aggcatcccc
     8281 gattatattc tataccgatg tggattgcgc atactttgtg aacagaaagt gatagcgttg
     8341 atgattcttc attggtcaga aaattatgaa cggtttcttc tattttgtct ctatatacta
     8401 cgtataggaa atgtttacat tttcgtattg ttttcgattc actctatgaa tagttcttac
     8461 tacaattttt ttgtctaaag agtaatacta gagataaaca taaaaaatgt agaggtcgag
     8521 tttagatgca agttcaagga gcgaaaggtg gatgggtagg ttatataggg atatagcaca
     8581 gagatatata gcaaagagat acttttgagc aatgtttgtg gaagcggtat tcgcaatatt
     8641 ttagtagctc gttacagtcc ggtgcgtttt tggttttttg aaagtgcgtc ttcagagcgc
     8701 ttttggtttt caaaagcgct ctgaagttcc tatactttct agagaatagg aacttcggaa
     8761 taggaacttc aaagcgtttc cgaaaacgag cgcttccgaa aatgcaacgc gagctgcgca
     8821 catacagctc actgttcacg tcgcacctat atctgcgtgt tgcctgtata tatatataca
     8881 tgagaagaac ggcatagtgc gtgtttatgc ttaaatgcgt acttatatgc gtctatttat
     8941 gtaggatgaa aggtagtcta gtacctcctg tgatattatc ccattccatg cggggtatcg
     9001 tatgcttcct tcagcactac cctttagctg ttctatatgc tgccactcct caattggatt
     9061 agtctcatcc ttcaatgcta tcatttcctt tgatattgga tcatactaag aaaccattat
     9121 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
     9181 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     9241 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     9301 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accataccac
     9361 cttttcaatt catcattttt tttttattct tttttttgat ttcggtttcc ttgaaatttt
     9421 tttgattcgg taatctccga acagaaggaa gaacgaagga aggagcacag acttagattg
     9481 gtatatatac gcaagcttgc ctcgtccccg ccgggtcacc cggccagcga catggaggcc
     9541 cagaataccc tccttgacag tcttgacgtg cgcagctcag gggcatgatg tgactgtcgc
     9601 ccgtacatct agcccataca tccccatgta taatcatttg catccataca ttttgatggc
     9661 cgcacggcgc gaagcaaaaa ttacggcccc tcgctgcgga cctgcgagca gggaaacgct
     9721 cccctcacag acgcgttgaa ttgtccccac gccgcgcccc tgtagagaaa tataaaaggt
     9781 taggatttgc cactgaggtt cttctttcat atacttcctt ttaaaatctt gctaggatac
     9841 agttctcaca tcacatccga acataaacaa ccatgggtaa aaagcctgaa ctcaccgcga
     9901 cgtctgtcga gaagtttctg atcgaacagt tcgacagcgt ctccgacctg atgcagctct
     9961 cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc
    10021 gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat
    10081 cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct
    10141 attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc
    10201 ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc
    10261 agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg
    10321 atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca
    10381 ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc
    10441 ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg
    10501 gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg
    10561 tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact
    10621 tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca
    10681 ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg
    10741 cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa
    10801 tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg
    10861 gaaaccgacg ccccagcact cgtccgaggg caaaggaata atcagtactg acaataaaaa
    10921 gattcttgtt ttcaagaact tgtcatttgt atagtttttt tatattgtag ttgttctatt
    10981 ttaatcaaat gttagcgtga tttatatttt ttttcgcctc gacatcatct gcccagatgc
    11041 gaagttaagt gcgcagaaag taatatcatg cgtcaatcgt atgtgaatgc tggtcgctat
    11101 actgctgtcg attcgatact aacgccgcca tccagtgtcg aatgatatcg aattcagtct
    11161 actagtcagt tcgagttta
//
