LOCUS       sequence-358423-       22583 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Saccharomyces cerevisiae inducible CRISPRai vector - MET17 marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 22583)
  AUTHORS   Shaw WM, Studena L, Roy K, Hapeta P, McCarty NS, Graham AE, Ellis T,
            Ledesma-Amaro R
  TITLE     Inducible expression of large gRNA arrays for multiplexed CRISPRai 
            applications.
  JOURNAL   Nat Commun. 2022 Aug 25;13(1):4984. doi: 10.1038/s41467-022-32603-7.
  PUBMED    36008396
REFERENCE   2  (bases 1 to 22583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-08-25"; volume: "13(1):4984. doi"; pages: " 
            10.1038/s41467-022-32603-7"
COMMENT     Sequence Label: pWS5394-CRISPRai-MET17
FEATURES             Location/Qualifiers
     source          1..22583
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1273..1293
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4999..5019
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5056..5076
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5110..5259
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             5284..5304
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5734..6090
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             7082..7102
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(7115..7138,7139..7141,7142..9628,9629..9631,
                     9632..11215)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             11231..11251
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             11264..11386
                     /codon_start=1
                     /product="transcriptional repressor domain from the mouse 
                     Max-interacting protein 1 (Schreiber-Agus et al., 1995)"
                     /label=Mxi1
                     /translation="MERVRMINVQRLLEAAEFLERRERECEHGYASSFPSMPSPR"
     CDS             12374..12394
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      12980..13167
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(13035..13059)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             14568..14588
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             16552..16572
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    17001..17019
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    17033..17051
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    17064..17082
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    17096..17114
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    17127..17145
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    17159..17177
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    17190..17208
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    17348..17367
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     RBS             17523..17534
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             17541..18254
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      18266..18337
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      18353..18380
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             18911..20245
                     /codon_start=1
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /product="O-acetylserine and O-acetylhomoserine 
                     sulfhydrylase, required for sulfur amino acid synthesis"
                     /label=MET17
                     /translation="MPSHFDTVQLHAGQENPGDNAHRSRAVPIYATTSYVFENSKHGSQ
                     LFGLEVPGYVYSRFQNPTSNVLEERIAALEGGAAALAVSSGQAAQTLAIQGLAHTGDNI
                     VSTSYLYGGTYNQFKISFKRFGIEARFVEGDNPEEFEKVFDERTKAVYLETIGNPKYNV
                     PDFEKIVAIAHKHGIPVVVDNTFGAGGYFCQPIKYGADIVTHSATKWIGGHGTTIGGII
                     VDSGKFPWKDYPEKFPQFSQPAEGYHGTIYNEAYGNLAYIVHVRTELLRDLGPLMNPFA
                     SFLLLQGVETLSLRAERHGENALKLAKWLEQSPYVSWVSYPGLASHSHHENAKKYLSNG
                     FGGVLSFGVKDLPNADKETDPFKLSGAQVVDNLKLASNLANVGDAKTLVIAPYFTTHKQ
                     LNDKEKLASGVTKDLIRVSVGIEFIDDIIADFQQSFETVFAGQKP"
     CDS             complement(20856..21671)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(20856..21671)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     21579..21598
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     21663..21682
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      21896..22484
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     22385..22404
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcggccgctc taattgtatc gagatcactt ttcgtgatcc gctaatcagc gacggtcaca
       61 ttaggtttgc caagtcaggg tatgaaccat acgatcagtt ttcgtgaacc tggtacgtat
      121 attgtggcgt ttgtgtatat tttcattctt tgacaacaat caataccaac ctcaaatagg
      181 aaaagtaata agtttggcgt tacaccccaa aagacgccaa acggatcgaa cttactcaat
      241 agcaattagc gagacaaaac ctacgttaag acctgtaacc gatttatcaa agcactctgc
      301 ggttctttct tgggaatatt acctggacat tttgtgccct caagaaacga ggctctacga
      361 gcctgttgga gcccctcaga cattagccgc cacgaatcaa actttttacg cgattcggcc
      421 caaatcagtt tctcacagat cattcgtaga gtgaaaaagc acatcgatta tttgataccc
      481 ctttgggtta attactgttg aggtctttgc cgtaagttcc atcgctgtct gaactggccg
      541 ataattgcag acgaacggtg ttgttatccg atacaaccgg atatttttct tttaatgagt
      601 ctaaaccgtg atagcttcag gttaatacaa tcaaaaaaag ctcaaatatt cttttaatgc
      661 cgcgttcaca gattccaatt gaatacaact aggtagttca ttatatgaag cctttgctac
      721 tatttttcac tatagtctgc cttcacctta atgcagacat ccacatattt taatcacttt
      781 aaaataaaaa ggaagatata ttagaagcta tgatccaatc tgtaagccag attaaaattc
      841 acgaactctt ctttcatttg aattgaatgc tttgagttgg ggtagattat cgcaaattac
      901 tcatcacatt tattgactac gaacttgctg atgtcctttt tttatttata tttttcttca
      961 gtgaagcgat ttttttttta cacagaccaa gacggaaaaa agtagctaag gaagaaaaca
     1021 aaatcatgaa aaaaatgtga agtgatcatg cacatcgcat caacttaaac attggcttag
     1081 agatatatag agttagagtt tacggcaacc tttaagcacc aatacctttt ggcatagtct
     1141 aaagacctgg ttcttaattt taaacaaatt taactaaaga tttccctatc aaagaagtaa
     1201 cgagttgaca gattttctca aaataaatcg atactgcatt tctaggcata tccagcgaga
     1261 tctatgtctg acccaaagaa gaagagaaag gttggttctg gttccaagtt ggaaaagttt
     1321 accaactgct actctctgtc taagaccttg agattcaaag ctattcctgt tggtaagacc
     1381 caagaaaaca ttgacaacaa gaggttgttg gttgaggacg aaaaaagagc tgaagattac
     1441 aagggtgtca agaagttgct ggacagatat tacttgtcct tcatcaacga tgtcctgcac
     1501 tctattaagc tgaagaactt gaacaactac atctccctgt tcagaaaaaa gaccagaacc
     1561 gagaaagaga acaaagagtt agaaaacttg gagatcaacc tgaggaaaga aattgctaag
     1621 gctttcaagg gtaacgaggg ttacaaatct ttgttcaaga aggacatcat cgaaaccatc
     1681 ttgccagaat ttttggatga taaggacgaa attgccttgg tcaattcttt caacggtttc
     1741 actactgctt tcactggttt ctttgacaac agggaaaaca tgttctctga agaagctaag
     1801 tctacctcca ttgctttcag atgcattaac gaaaacttga ccaggtacat ctccaacatg
     1861 gatattttcg aaaaggttga tgccatcttc gataagcacg aagtccaaga aatcaaagag
     1921 aagattttga actccgatta cgacgtcgaa gattttttcg aaggtgagtt cttcaacttc
     1981 gttttgactc aagaaggtat cgacgtttac aacgctatta ttggtggttt cgttaccgaa
     2041 tctggtgaaa agattaaggg tttgaacgag tacatcaacc tgtacaatca aaagaccaag
     2101 cagaagttgc caaagtttaa gcccttgtac aaacaagtct tgtccgacag agaatccttg
     2161 tctttttatg gtgaaggtta cacctccgat gaagaagttt tggaagtttt cagaaacacc
     2221 ctgaacaaga actccgaaat cttctcatcc attaagaagt tggagaagct gtttaagaac
     2281 ttcgacgaat attcctccgc tggtatcttt gtcaaaaatg gtccagctat ttccaccatc
     2341 tccaaggata tttttggtga atggaacgtc atcagagata agtggaatgc tgaatacgat
     2401 gacatccact tgaaaaagaa ggctgttgtt accgaaaagt acgaggatga tagacgtaag
     2461 tccttcaaga agattggctc tttctctctg gaacaattgc aagaatatgc tgatgccgat
     2521 ttgtccgttg tcgaaaaatt gaaagaaatc atcatccaga aggtcgacga aatctacaaa
     2581 gtttacggtt cctctgaaaa gttgttcgat gctgatttcg ttttggagaa gtcgttgaag
     2641 aaaaacgatg ctgttgttgc catcatgaag gatttgttgg attccgttaa gagcttcgag
     2701 aactacatta aggctttttt cggtgagggc aaagaaacta acagagatga atcattctac
     2761 ggcgattttg ttttggccta cgatattttg ttgaaggttg atcatatcta cgacgccatc
     2821 agaaactacg ttactcaaaa accatactcc aaagacaagt tcaagctgta ctttcaaaac
     2881 ccacaattca tgggtggttg ggacaaagac aaagaaacag attacagagc caccatcttg
     2941 agatatggtt ctaagtacta cttggccatc atggataaga aatacgctaa gtgcttgcaa
     3001 aagatcgata aggatgatgt caacggtaac tacgagaaga tcaactacaa attattgccc
     3061 ggtccaaaca agatgttgcc taaggttttc ttcagcaaaa agtggatggc ttactacaac
     3121 ccatccgaag atattcaaaa gatttacaag aacggcacct tcaaaaaggg tgatatgttc
     3181 aacttgaacg actgccataa gctgatcgat ttcttcaagg attccatctc aagataccca
     3241 aagtggtcta atgcttacga tttcaacttc tctgaaaccg agaagtacaa agatattgcc
     3301 ggtttttaca gggaagtcga agaacaaggt tacaaggttt cattcgaatc cgcctctaaa
     3361 aaagaagttg acaagttggt tgaagagggc aagttgtaca tgttccagat ttataacaag
     3421 gacttctccg ataagtctca tggtactcca aacttgcata ccatgtactt caagttgttg
     3481 ttcgacgaaa acaatcacgg tcagattaga ttgtctggtg gtgctgagtt gtttatgaga
     3541 agggcttctt tgaagaaaga agagttggtt gttcatccag ccaattctcc aattgctaac
     3601 aagaatccag acaacccaaa aaagactacc accttgtctt acgatgttta caaggataag
     3661 agattctccg aggaccaata cgaattgcat attccaattg ccattaacaa gtgccccaag
     3721 aacattttca agatcaacac cgaagttagg gtgttgttga agcacgatga taacccatac
     3781 gttattggta ttgctagggg tgaaagaaac ttgttgtaca tcgttgttgt tgatggtaag
     3841 ggtaacatcg tggaacaata ctcattgaac gagattatca acaacttcaa cggcatcaga
     3901 atcaagaccg attaccattc tctgcttgac aagaaagaaa aagaaagatt cgaagccaga
     3961 cagaactgga cctctattga aaacatcaaa gagttgaagg ccggttacat ttcacaagtt
     4021 gttcataaga tttgcgagct ggttgaaaag tatgatgcag ttattgcttt ggaggacttg
     4081 aactctggtt tcaagaactc cagagttaag gtcgaaaagc aggtctacca aaagttcgaa
     4141 aagatgttga tcgacaagct gaactacatg gtcgacaaaa agtctaatcc atgtgctaca
     4201 ggtggtgctt tgaaaggtta tcaaattacg aacaagttcg agagcttcaa gtctatgtct
     4261 actcagaatg gtttcatctt ctacattcca gcttggttga cctctaagat tgatccatct
     4321 actggtttcg tcaacctgct gaaaactaag tacacttcta ttgccgactc caagaagttc
     4381 atctcttcat tcgatagaat catgtacgtt ccagaagagg acttattcga atttgctttg
     4441 gattacaaga acttctcaag aactgatgct gactacatca agaagtggaa gttgtactct
     4501 tacggtaaca gaatcaggat cttcagaaac cctaagaaga acaacgtttt cgattgggaa
     4561 gaagtctgtt tgacctctgc ttacaaagaa ctgtttaaca agtacggcat caactaccaa
     4621 caaggtgata ttagagcttt gttgtgcgaa caatctgata aggccttcta ctcatctttt
     4681 atggccttga tgtcactgat gttgcagatg agaaattcca ttactggtag aaccgatgtc
     4741 gactttttga tctctccagt taagaactct gacggtattt tctacgactc aagaaactat
     4801 gaagctcaag aaaacgccat tttgccaaaa aacgctgatg ctaatggtgc ttacaacatt
     4861 gctagaaaag ttttgtgggc tatcggccaa ttcaaaaaag cagaagatga gaagctggac
     4921 aaggttaaga ttgccatctc aaacaaagaa tggttggaat acgctcagac ttctgtcaaa
     4981 catggttctg gttctccacc aaagaagaaa agaaaggttg gttccggttc taaattaggt
     5041 ggttctggtg gttccccaaa gaagaaaaga aaggttggta gagctgaagc ttctggttca
     5101 ggtagagcag atgctttgga tgatttcgat ttggacatgt tgggttctga tgcattggac
     5161 gatttcgact tagatatgtt aggtagtgac gccttggatg acttcgatct ggatatgcta
     5221 ggttccgatg ccttagacga ctttgattta gacatgctga ttaactcaag atcctctggt
     5281 tctcctaaga agaagagaaa agtcggttct caatacttgc cagataccga tgatagacac
     5341 agaatcgaag aaaagaggaa gagaacttac gaaaccttca agtccatcat gaagaagtct
     5401 ccattttctg gtccaactga tccaagacca ccaccaagaa gaattgctgt tccttcaaga
     5461 tcctctgcct ctgttccaaa accagctcca caaccttatc cttttacatc ttctttgtcc
     5521 accatcaact acgatgaatt tccaactatg gttttcccat ccggtcaaat ttctcaagct
     5581 tctgctttgg ctccagctcc tccacaagtt ttgccacaag ctccagcacc agctccagct
     5641 ccagcaatgg tttctgcttt agctcaagcc ccagctcctg ttccagtttt agctccaggt
     5701 ccacctcaag ctgttgctcc accagcacca aaacctactc aagctggtga aggtactttg
     5761 tctgaagctt tgttgcagtt gcaattcgat gatgaagatt tgggtgcttt gttgggtaat
     5821 tctactgatc cagctgtttt taccgatttg gcttctgttg ataactccga atttcagcag
     5881 ttgttgaatc aaggtattcc agttgctcct catacaactg aacctatgtt gatggaatat
     5941 ccagaagcaa ttactagatt agttactggt gctcaaagac ctccagatcc tgcacctgct
     6001 cctttgggtg ctccaggttt accaaatggt ctattgtctg gtgacgaaga tttttcctct
     6061 attgccgata tggatttctc cgctttgttg tgaggatcct aactcgagag tgcttttaac
     6121 taagaattat tagtcttttc tgcttatttt ttcatcatag tttagaacac tttatattaa
     6181 cgaatagttt atgaatctat ttaggtttaa aaattgatac agttttataa gttacttttt
     6241 caaagactcg tgctgtctat tgcataatgc actggaaggg gaaaaaaaag gtgcacacgc
     6301 gtggcttttt cttgaatttg cagtttgaaa aatgctgcca aaccagatgt caacacagct
     6361 acaacgtgac ccaacatcag atgacccaag gtccacctct tattaaagga cgtttgatcc
     6421 ttcgacacca tggctctgtt gaacttttat ctgagagagg aaaaaaagga aggaaaaaaa
     6481 agaagaaact tcctttattt atttgtctta accacaacac acaatgcaat aagatgcaat
     6541 ataatatcaa agccaatatc ttatgttgct gatcctgaga aggaatatat acaatttatg
     6601 tagtaaaata ccttttcttc tgcgagttgc aagaaataga aaagactccg attgcgcatc
     6661 gccagaataa aatttcacaa ccacactttt tggctgaact ttttattacc tgattaaaca
     6721 gagagagaaa aggtagaggt caaaattttt taagcaaaac taaaaaagat gcaaaatcac
     6781 gtgctgaaaa tctaacataa gggttaagat tagagtttta taggacttgt tttgtaatat
     6841 ttcaaatacg agctaaccct actgatttca attaggtcta atttagggtt gagctgcact
     6901 gaaatttcgg aaattttggg ttattttaaa tgagacagaa gaactacaga gatacgttct
     6961 tcagacttta aagcttatct ccacaaagaa ttggtcaaga aatcatccta gaaaaacacg
     7021 tttgctcact cgatcttaat cacatagagt gctggaacgg gaagaaagat ctatgtctga
     7081 tccaaaaaag aagagaaagg tcggttctgg ttctgacaag aaatactcta ttggtttggc
     7141 tattggcacc aattctgttg gttgggctgt tattactgac gagtacaaag ttccctccaa
     7201 gaagtttaag gttttgggta acactgacag gcactccatt aagaaaaatt tgattggtgc
     7261 cctgttgttc gattctggtg aaactgctga agctactaga ttgaaaagaa ccgctagaag
     7321 aagatacacc agaagaaaga acagaatctg ctacttgcaa gagattttct ctaacgaaat
     7381 ggccaaggtt gatgattcat tcttccacag attggaagag tccttcttgg ttgaagagga
     7441 taagaaacac gaaagacatc caatcttcgg taacatcgtt gatgaagttg cttaccacga
     7501 aaagtaccca actatctacc atttgagaaa gaagttggtt gactctaccg ataaggctga
     7561 tttgagattg atctatttgg ctttggccca catgattaag ttcagaggtc atttcttgat
     7621 cgagggtgat ttgaatccag ataactccga tgttgacaag ttgttcatcc aattggtcca
     7681 aacttacaac cagttgttcg aagaaaatcc aatcaatgcc tctggtgttg atgctaaggc
     7741 tattttgtct gctagactgt ctaagtcaag aaggttggaa aacttgattg ctcaattgcc
     7801 aggtgaaaag aagaatggtt tgttcggtaa tttgatcgcc ttgtctttgg gtttgactcc
     7861 aaatttcaag tccaacttcg atttggctga agatgctaag ttgcaattgt ctaaggatac
     7921 ctacgatgat gacttggata atttgttggc ccaaatcggt gatcaatacg ctgatttgtt
     7981 tttggctgct aagaacttgt ccgatgccat tttgttgtcc gatatcttga gagttaacac
     8041 cgaaattact aaggctccat tgtctgcctc tatgatcaaa agatacgatg aacaccacca
     8101 agacttgact ttgttgaaag ctttggtcag acaacagttg ccagaaaagt acaaagaaat
     8161 cttcttcgac cagtccaaaa atggttacgc tggttatatt gatggtggtg cttctcaaga
     8221 agagttctac aagttcatta agcccatctt ggaaaagatg gatggtactg aagaactgtt
     8281 ggtcaagttg aacagagaag atttgttgag aaagcagaga accttcgata acggttctat
     8341 tccacatcaa atccacttgg gtgaattgca tgctattttg agaaggcaag aggatttcta
     8401 cccattcttg aaggataaca gagaaaagat cgagaagatc ctgaccttca gaatcccata
     8461 ttatgttggt ccattggcca gaggtaattc aagatttgct tggatgacta ggaagtccga
     8521 agaaactatt actccatgga acttcgaaga ggttgttgat aagggtgctt ctgctcaatc
     8581 cttcattgaa agaatgacta acttcgacaa gaacctgcca aacgaaaagg ttttgccaaa
     8641 acactctttg ctgtacgaat acttcaccgt ctacaacgaa ttgactaagg ttaagtacgt
     8701 caccgaaggt atgagaaaac cagctttttt gtctggtgaa caaaagaagg ctatcgtcga
     8761 tttgttgttc aagactaaca gaaaggttac cgtcaagcaa ttgaaagagg actacttcaa
     8821 gaaaatcgag tgcttcgact ccgttgaaat tagtggtgtt gaagatagat tcaacgcctc
     8881 cttaggtact taccatgact tattgaagat catcaaggac aaggatttct tggacaacga
     8941 agaaaacgag gatatcttgg aggatatcgt tttgactttg accttgttcg aggataggga
     9001 aatgatcgaa gaaaggttga aaacctacgc tcacttgttt gatgataagg tcatgaagca
     9061 gttgaagaga agaaggtata ctggttgggg tagattgtct aggaaattga ttaacggtat
     9121 cagggacaag caatccggta agactatttt ggactttttg aagtctgatg gcttcgccaa
     9181 tagaaacttc atgcaattga tccacgatga cagcttgacc ttcaaagaag atattcaaaa
     9241 ggcccaagtt tccggtcaag gtgattcttt acatgaacat attgctaact tggctggttc
     9301 cccagctatt aagaaaggta ttttacaaac cgttaaggtg gtcgacgaat tggtaaaagt
     9361 tatgggtaga cataagccag agaacatcgt tattgaaatg gctagagaaa atcagactac
     9421 ccaaaagggt caaaagaact ccagagaaag gatgaagaga attgaagagg gtatcaaaga
     9481 attgggctcc cagattttga aagaacaccc agttgaaaac acccaattgc agaacgaaaa
     9541 gctgtacttg tactacttgc agaatggtag agatatgtac gtcgaccaag aattggatat
     9601 caacagattg tccgattacg atgtcgatgc tatcgttcca cagtcttttt tgaaggatga
     9661 ctccatcgat aacaaggtct tgactagatc cgataagaac agaggtaagt ctgataacgt
     9721 tccatctgaa gaggtcgtca agaagatgaa gaattattgg agacagttgc tgaacgccaa
     9781 gttgattact caaagaaagt tcgacaattt gaccaaggct gaaagaggtg gtttgtctga
     9841 attggacaaa gcaggtttca ttaagaggca attggttgaa actaggcaaa tcaccaaaca
     9901 cgttgcccaa attttggatt ccagaatgaa taccaagtac gacgaaaacg ataagttgat
     9961 cagggaagtt aaggtcatca cgttgaagtc taagttggtt tccgatttca gaaaggactt
    10021 ccaattctac aaggtcaggg aaatcaacaa ctaccatcat gctcatgatg cttacttgaa
    10081 tgctgttgtt ggtactgcct tgattaagaa gtatccaaag ttggaatccg agttcgttta
    10141 cggtgattac aaggtttacg acgtcagaaa gatgattgcc aagtccgaac aagaaattgg
    10201 taaggctact gctaagtact tcttctactc caacatcatg aatttcttca agaccgaaat
    10261 cactttggcc aacggtgaaa ttagaaaaag gccattgatt gagactaacg gtgagactgg
    10321 tgaaatagtt tgggataagg gtagagattt cgctaccgtt agaaaagttt tgtccatgcc
    10381 acaagttaac atcgtcaaaa agaccgaagt tcaaactggt ggtttctcca aagaatccat
    10441 cttgccaaaa agaaactccg acaaattgat cgccagaaag aaagattggg acccaaaaaa
    10501 gtatggtggt tttgattctc caaccgttgc ttactctgtt ttggttgttg ctaaagttga
    10561 aaagggcaag agcaagaagt tgaagtcagt caaagaacta ttgggcatca cgatcatgga
    10621 aaggtcatct tttgaaaaaa acccaatcga ctttttggag gccaagggtt acaaagaagt
    10681 taagaaggat ctgattatca agctgcccaa gtactctttg ttcgaattgg aaaacggtcg
    10741 taagagaatg ttggcttctg ctggtgaatt acaaaagggt aatgaattgg ctctgccctc
    10801 taagtacgtt aactttctat acttggcctc tcactacgaa aagttgaagg gttctcctga
    10861 agataacgaa caaaagcagt tgtttgtcga acagcataag cactacttgg acgaaatcat
    10921 cgaacagatt tctgaatttt ccaagagggt tattttggct gatgccaatt tggataaggt
    10981 cttgtctgct tacaacaagc acagagataa gccaattaga gaacaagccg aaaacatcat
    11041 tcacctgttc actttgacta atttgggtgc tccagctgct tttaagtatt tcgataccac
    11101 catcgacaga aagagataca cttctacaaa agaggttctg gacgctacct tgattcatca
    11161 atctattact ggcttgtacg aaaccaggat cgacttgtct caattaggtg gtgatggttc
    11221 aggttctcca ccaaagaaaa aacgtaaggt cggatctggt tctatggaac gtgtgagaat
    11281 gattaatgtg caaaggctgt tagaagccgc agagttttta gaaagaagag aaagagaatg
    11341 cgaacacggg tatgccagtt ctttccctag catgccctct cccagaggat cctaactcga
    11401 gattgaattg aattgaaatc gatagatcaa tttttttctt ttctctttcc ccatccttta
    11461 cgctaaaata atagtttatt ttattttttg aatatttttt atttatatac gtatatatag
    11521 actattattt atcttttaat gattattaag atttttatta aaaaaaaatt cgctcctctt
    11581 ttaatgcctt tatgcagttt ttttttccca ttcgatattt ctatgtgctg gatgcacaca
    11641 ctggcttaag atgacaacgt atatattaaa tttgctcttg ttctgtactt tcctaattct
    11701 tatgtaaaaa gacaagaatt tatgatacta tttaataaca aaaaactacc taagaaaagc
    11761 atcatgcagt cgaaattgaa atcgaaaagt aaaactttaa cggaacatgt ttgaaattct
    11821 aagaaagcat acatcttcat cccttatata tagagttatg tttgatatta gtagtcatgt
    11881 tgtaatctct ggcctaagta tacgtaacga aaatggtagc acgtcgcgtt tatggccccc
    11941 aggttaatgt gttctctgaa attcgcatca ctttgagaaa taatgggaac accttacgcg
    12001 tgagctgtgc ccaccgcttc gcctaataaa gcggtgttct caaaatttct ccccgttttc
    12061 aggatcacga gcgccatcta gttctggtaa aatcgcgctt acaagaacaa agaaaagaaa
    12121 catcgcgtaa tgcaacagtg agacacttgc cgtcatatat aaggttttgg atcagtaacc
    12181 gttatttgag cataacacag gtttttaaat atattattat atatcatggt atatgtgtaa
    12241 aatttttttg ctgactggtt ttgtttattt atttagcttt ttaaaaattt tactttcttc
    12301 ttgttaattt tttctgattg ctctatactc aaaccaacaa caacttactc tacaactaag
    12361 atctatgtct gacccaaaga aaaaaagaaa agttggttca ggttctgacc actacttgga
    12421 tattagatta agaccagatc cagaatttcc accagctcaa ttgatgtctg ttttgttcgg
    12481 taaattgcac caagctttgg ttgctcaagg tggtgataga attggtgttt catttccaga
    12541 tttggacgaa tccagatcaa gattgggtga aagattgaga atccatgctt ccgctgatga
    12601 tttgagagct ttgttggcta gaccatggtt ggaaggtttg agagatcact tgcaatttgg
    12661 tgaaccagct gttgttccac atccaactcc atatagacaa gtttcaagag ttcaagccaa
    12721 gtctaaccca gaaagattgc gtagaagatt gatgagaagg cacgatttgt ctgaagaaga
    12781 agccagaaaa agaatcccag atactgttgc tagagctttg gatttgccat tcgttacttt
    12841 gagatcacaa tctaccggtc aacatttcag gttgttcatt agacatggtc cattgcaagt
    12901 tactgctgaa gaaggtggtt ttacttgtta cggtttgtct aaaggtggat tcgttccatg
    12961 gtttggatcc taactcgagg cgaatttctt atgatttatg atttttatta ttaaataagt
    13021 tataaaaaaa ataagtgtat acaaatttta aagtgactct taggttttaa aacgaaaatt
    13081 cttattcttg agtaactctt tcctgtaggt caggttgctt tctcaggtat agcatgaggt
    13141 cgctcttatt gaccacacct ctaccggcat gccgagcaaa tgcctgcaaa tcgctcccca
    13201 tttcgctggt tctgtatggg cacagacaac ctaaacgcct tgtgaaattg caaatatggt
    13261 gatttgaaac gtttcctagt gcagcaggat cacagataac gtgtaaaggg cttagcagtt
    13321 gataatcctc tctagttaag acctaaacaa aatgctgtca ctaaccgtag tattaaatga
    13381 cacactttgg tgactttcgt taatggggat gtggtagtgg ccattgccaa taaacaaaaa
    13441 gaacagggaa agaagtagaa agtgatataa gtttgcttgc cacttttcgt ttttcacgaa
    13501 aaaaacaggc gaaaaaaaat gctagacaag tacccggctg aatcacacct cgttaacagt
    13561 gactttcggt gacagatacc cgattgggca cccggctggt aagttatgat agaaagccaa
    13621 cgctgtacta ttggcttagc tatggcaata ttttgattat cagctagttt tattaacgtt
    13681 ataattagtg taaccagttt ttcatctatt tcatttattt catttattta ctttaattgc
    13741 agatccccct aacgcgttta aagcttttat tcactagctt atgtattttt tataggaaac
    13801 gcgacgcgta acatcgcgca aatgaaggtt ttgatgtatt ataatgaggt attcttcctt
    13861 atatacatcg atgaaaagcg ttgacagcat acattggaaa gaaataggaa acggacaccg
    13921 gaagaaaaaa tagatctatg tcaagattgg acaaaagcaa ggttattaac ggtgccttgg
    13981 aactgttgaa tggtgtaggt atagaaggct taacgaccag aaaattagca caaaagttag
    14041 gtgtagagca gccaacatta tattggcacg tcaaaaacaa gagagcattg ctagacgcat
    14101 tgccaatcga aatgttagat cgtcatcaca ctcatttttg cccattagag ggtgagagtt
    14161 ggcaagactt tttgcgtaac aatgctaaat cctttagatg cgccttgttg agccatcgtg
    14221 atggtgccaa ggttcatcta ggtacaaggc caacagaaaa acagtatgag actttggaga
    14281 atcagctagc ctttttatgc caacagggtt tttcattgga gaatgcctta tacgctttaa
    14341 gtgcagttgg tcacttcact ttaggttgcg tactagaaga acaagagcac caggttgcta
    14401 aagaagagag agaaacacct acaacagatt caatgcctcc attgctaaga caagctatcg
    14461 aactgtttga tagacagggt gcagaacctg cattcttgtt cggtttagag ttgattattt
    14521 gcggcttgga aaagcagcta aaatgcgaat ctggtagtgg ttcacctcct aagaaaaaga
    14581 gaaaagttgg ttccggtagc gctaatttca atcagtctgg taatattgcc gactcctctt
    14641 tgtctttcac tttcactaat tcttccaacg gtccaaactt gatcactacc caaactaatt
    14701 ctcaagcttt gtctcagcca attgcctctt ctaatgttca cgataacttc atgaacaacg
    14761 aaatcaccgc ctccaaaatt gatgatggta acaattctaa gccattgtct ccaggttgga
    14821 ctgatcaaac tgcttataat gctttcggta ttaccaccgg tatgtttaac actactacta
    14881 tggatgacgt ctacaactac ttgttcgatg atgaagatac accaccaaat ccaaagaaag
    14941 aaggatccta actcgaggcc aattggtgcg gcaattgata ataacgaaaa tgtcttttaa
    15001 tgatctgggt ataatgagga attttccgaa cgtttttact ttatatatat atatacatgt
    15061 aacatatatt ctatacgcta tagagaaagg aaatttttca attaaaaaaa aaatagagaa
    15121 agagtttcac ttcttgatta tcgctaacac taatggttga agtactgcta ctttaatttt
    15181 atgctgggta gactacccat gagtcacaat gaacgttcgt gctttgtgat aaagtgtttc
    15241 acgtcatccg acatgacttc gtagttatgg actgaactgt gtggtgaggt tccatgattt
    15301 cttaggtcca gcagatacat gtctcttccc aatttcttgt taaggttacg gccaatgctt
    15361 cggttgttga gcttgttacc gaataagccg tgaagtatga taataggtgg tcttggcttc
    15421 ccttcatccc cagtttttac tgcatctctc ttgattatgt catatgaaag gtccagtggg
    15481 acttgctttt gttgcagcac ctttgctaat gaatgaaagg cacatagtga ctgcttaaaa
    15541 atgcaggaac ttaaattatt ccgaatggta ttttgtctca catatattgt cccatactgt
    15601 gccaagatcc cggctttacc cagtatcatc attgtaccgt taccaattct cctcgtatat
    15661 cacggttagt ttttaaacct cggggtgacg tttactattg gcgtactaat atattcttat
    15721 tttcttttct tttttgttgg cagtttcaag caacacatgt actggataac caacccccgc
    15781 acgctcttgg aaaaaattga gaaggcatcg gacacttgct gatgagtatt tcgaaaaatt
    15841 ccatgaagat gaggccaaga ttgtttggaa gagattgaaa agaagaagaa gaaaaaaaga
    15901 taaaagcaaa tcaaaagatc tatgtcaaga ttggataagt ccaaggttat caactctgct
    15961 ttggagttgt tgaacgaagt tggtattgaa ggtttgacca ctagaaagtt ggctcaaaaa
    16021 ttgggtgttg ctcaaaaaac cttgtactgg cacgttaaga acaaaagagc tttgttggac
    16081 gctttggcca ttgaaatgtt ggatagacat catacccatt tctgtccatt ggaaggtgaa
    16141 tcatggcaag atttcttgag aaacaacgcc aagtctttca gatgtgcttt gttgtctcat
    16201 agagatggtg ctaaagttca cttgggtact agaccaactg aaaagcaata cgaaactttg
    16261 gaaaaccagt tggctttctt gtgtcaacaa ggtttctctt tagaaaacgc cttgtatgct
    16321 ttgtctgctg ttggtcattt taccttgggt tgtgttttgg aagatcaaga acatcaagtc
    16381 gccaaagaag aaagggaaac tccaactact gattctatgc caccattatt gagacaagcc
    16441 attgagttgt ttgatcatca aggtgctgaa ccagcttttt tgtttggttt ggaattgatc
    16501 atctgcggtt tggagaaaca attgaagtgt gaatctggtt ctggttctcc accaaaaaag
    16561 aaaagaaagg ttggttcagg ttccgaaagg gttagaatga ttaacgttca aaggttgttg
    16621 gaagctgccg aatttttgga aagacgtgaa agggaatgtg aacatggtta cgcttcatct
    16681 tttccatcta tgccatctcc aagaggatcc taactcgaga gcttttgatt aagccttcta
    16741 gtccaaaaaa cacgtttttt tgtcatttat ttcattttct tagaatagtt tagtttattc
    16801 attttatagt cacgaatgtt ttatgattct atatagggtt gcaaacaagc atttttcatt
    16861 ttatgttaaa acaatttcag gtttaccttt tattctgctt gtggtgacgc gtgtatccgc
    16921 ccgctctttt ggtcacccat gtatgctgaa gtgaataaag ctccacacag tcgaacgggt
    16981 agtccatcgt tgtaggatac tccctatcag tgatagagaa atctatgcgg catccctatc
    17041 agtgatagag atcggtaacg gagtccctat cagtgataga gaagtggtgt tcagttccct
    17101 atcagtgata gagaagtttg acactatccc tatcagtgat agagagaaca gcaatgactc
    17161 cctatcagtg atagagatca aatggcgtat ccctatcagt gatagagata attaacttgt
    17221 aatattctaa tcaattgata taaaaatccc cgtcagtgac ggagattttc tttttttttt
    17281 atttttcccc gtcagtgacg gagaatatca agctacaaaa agaattcgca tctagattct
    17341 tgagacggtt cactgccgta taggcagtga gaccgaaagt gaaacgtgat ttcatgcgtc
    17401 attttgaaca ttttgtaaat cttatttaat aatgtgtgcg gcaattcaca tttaatttat
    17461 gaatgttttc ttaacatcgc ggcaactcaa gaaacggcag gttcggatct tagctactag
    17521 agaaagagga gaaatactag atgcgtaaag gcgaagagct gttcactggt gtcgtcccta
    17581 ttctggtgga actggatggt gatgtcaacg gtcataagtt ttccgtgcgt ggcgagggtg
    17641 aaggtgacgc aactaatggt aaactgacgc tgaagttcat ctgtactact ggtaaactgc
    17701 cggttccttg gccgactctg gtaacgacgc tgacttatgg tgttcagtgc tttgctcgtt
    17761 atccggacca tatgaagcag catgacttct tcaagtccgc catgccggaa ggctatgtgc
    17821 aggaacgcac gatttccttt aaggatgacg gcacgtacaa aacgcgtgcg gaagtgaaat
    17881 ttgaaggcga taccctggta aaccgcattg agctgaaagg cattgacttt aaagaggacg
    17941 gcaatatcct gggccataag ctggaataca attttaacag ccacaatgtt tacatcaccg
    18001 ccgataaaca aaaaaatggc attaaagcga attttaaaat tcgccacaac gtggaggatg
    18061 gcagcgtgca gctggctgat cactaccagc aaaacactcc aatcggtgat ggtcctgttc
    18121 tgctgccaga caatcactat ctgagcacgc aaagcgttct gtctaaagat ccgaacgaga
    18181 aacgcgatca tatggttctg ctggagttcg taaccgcagc gggcatcacg catggtatgg
    18241 atgaactgta caaatgacca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
    18301 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
    18361 ttcgggtggg cctttctgcg tttataggtc tcatccccgt cagtgacgga gatcgtctct
    18421 accaactagt gcactgcagt acaataaagc aatcttgatg aggataatga tttttttttg
    18481 aatatacata aatactaccg tttttctgct agattttgtg atgacgtaaa taagtacata
    18541 ttacttttta agccaagaca agattaagca ttaactttac ccttttcttt ctaagtttca
    18601 atattagtta tcactgttta aaagttatgg cgagaacgtc ggcggttaaa atatattacc
    18661 ctgaacgagc atacacgcca tcctcatgaa aactgtgtaa cataataacc gaagtgtcga
    18721 aaaggtggca ccttgtccaa ttgaacacgc tcgatgaaaa aaataagata tatataaggt
    18781 taagtaaagc gtctgttaga aaggaagttt ttcctttttc ttgctctctt gtcttttcat
    18841 ctactatttc cttcgtgtaa tacagggtcg tcagatacat agatacaatt ctattacccc
    18901 catccataca atgccatctc atttcgatac tgttcaacta cacgccggcc aagagaaccc
    18961 tggtgacaat gctcacagat ccagagctgt accaatttac gccaccactt cttatgtttt
    19021 cgaaaactct aagcatggtt cgcaattgtt tggtcttgaa gttccaggtt acgtctattc
    19081 ccgtttccaa aacccaacca gtaatgtttt ggaagaaaga attgctgctt tagaaggtgg
    19141 tgctgctgct ttggctgttt cctccggtca agccgctcaa acccttgcca tccaaggttt
    19201 ggcacacact ggtgacaaca tcgtttccac ttcttactta tacggtggta cttataacca
    19261 gttcaaaatc tcgttcaaaa gatttggtat cgaggctaga tttgttgaag gtgacaatcc
    19321 agaagaattt gaaaaggtct ttgatgaaag aaccaaggct gtttatttgg aaaccattgg
    19381 taatccaaag tacaatgttc cggattttga aaaaattgtt gcaattgctc acaaacacgg
    19441 tattccagtt gtcgttgaca acacatttgg tgccggtggt tacttctgtc agccaattaa
    19501 atacggtgct gatattgtaa cacattctgc taccaaatgg attggtggtc atggtactac
    19561 tatcggtggt attattgttg actctggtaa gttcccatgg aaggactacc cagaaaagtt
    19621 ccctcaattc tctcaacctg ccgaaggata tcacggtact atctacaatg aagcctacgg
    19681 taacttggca tacatcgttc atgttagaac tgaactatta agagatttgg gtccattgat
    19741 gaacccattt gcctctttct tgctactaca aggtgttgaa acattatctt tgagagctga
    19801 aagacacggt gaaaatgcat tgaagttagc caaatggtta gaacaatccc catacgtatc
    19861 ttgggtttca taccctggtt tagcatctca ttctcatcat gaaaatgcta agaagtatct
    19921 atctaacggt ttcggtggtg tcttatcttt cggtgtaaaa gacttaccaa atgccgacaa
    19981 ggaaactgac ccattcaaac tttctggtgc tcaagttgtt gacaatttaa agcttgcctc
    20041 taacttggcc aatgttggtg atgccaagac cttagtcatt gctccatact tcactaccca
    20101 caaacaatta aatgacaaag aaaagttggc atctggtgtt accaaggact taattcgtgt
    20161 ctctgttggt atcgaattta ttgatgacat tattgcagac ttccagcaat cttttgaaac
    20221 tgttttcgct ggccaaaaac catgagtgtg cgtaatgagt tgtaaaatta tgtataaacc
    20281 tactttctct cacaaggagt tatcgtgttg catctgcggc tttaaattga tgtatctcat
    20341 cgcaggcacg ggcagtacag tgccctgagc gtagggaaaa atgaaaaaaa ggatgtaact
    20401 tttaacataa ttccagcacg cagcgattgg gtataatgaa gattgttaag ttcaacaaca
    20461 tgtgaaattc ttagctccgc aacttgcttg aattatgagc tctaagattc aagaagtaaa
    20521 taggtcatat aatggaatga tagtagtact tgataaagga accatgtgat cttacgttga
    20581 tatgaaaata attctctcac agaagtcagt ttgtatgact aacatagaga gtaattgctt
    20641 ttcggataaa atcgcaccta acaaagatta ccttttgtaa agcctccaga acagctatga
    20701 atgttcgtta ttctacgcac tttctttcat acatcttgat ccgccttttt tgatgtaggt
    20761 tttaacgcgg caaaacatat taatttttct gatttggaaa aataaagagt tagttctgcc
    20821 gcggccgcgt gttacaacca attaaccaat tctgattaga aaaactcatc gagcatcaaa
    20881 tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc
    20941 tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg
    21001 tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata
    21061 aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagc
    21121 ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca
    21181 ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgaggcg aaatacgcga
    21241 tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc
    21301 agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt
    21361 ttcccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg
    21421 atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca
    21481 tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca
    21541 tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca
    21601 tataaatcag catccatgtt ggaatttaat cgcggcctgg agcaagacgt ttcccgttga
    21661 atatggctca taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat
    21721 gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac aacgtggctt
    21781 tgttgaataa atcgaacttt tgctgagttg aaggatcagt catgaccaaa atcccttaac
    21841 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    21901 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    21961 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    22021 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
    22081 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    22141 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    22201 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    22261 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    22321 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    22381 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    22441 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    22501 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
    22561 cccctgattc tgtggataac cgt
//
