LOCUS       sequence-358628-        8499 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector encoding a protein fusion composed of 
            human cathepsin D fused to to one tyrosine sulfation (TS) motif (Y),
            myc (M) and His6 (H) epitope.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8499)
  AUTHORS   Buser DP, Bader G, Spiess M
  TITLE     Retrograde transport of CDMPR depends on several machineries as 
            analyzed by sulfatable nanobodies.
  JOURNAL   Life Sci Alliance. 2022 Mar 21;5(7). pii: 5/7/e202101269. doi: 
            10.26508/lsa.202101269. Print 2022 Mar.
  PUBMED    35314489
REFERENCE   2  (bases 1 to 8499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2022-03-21"; volume: "5(7). pii: 5/7/e202101269. 
            doi"; pages: " 10.26508/lsa.202101269. Print 2022 Mar"
COMMENT     Sequence Label: pQCXIP-cathepsinD-YMH
FEATURES             Location/Qualifiers
     source          1..8499
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1604..1907
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1908..2111
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2108..2132
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3533..3562
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3563..3580
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    join(3626..4200,4201..4203,4204..4212)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3793..3810)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4020..4039
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4232..4831
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4232..4251)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4728..4748
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5205..5630
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(5733..5755)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5891..5911)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5896..6225
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6076..6211
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6247..6266
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6347..6364)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6518..7106)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6598..6617)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7277..8068,8069..8137))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7900..7919
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8138..8242)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8310..8328
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(8428..8499,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg
     1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1921 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
     2221 cggacctgca ggaattgatc cgcggccgcc cgccaccatg cagccctcca gccttctgcc
     2281 gctcgccctc tgcctgctgg ctgcacccgc ctccgcgctc gtcaggatcc cgctgcacaa
     2341 gttcacgtcc atccgccgga ccatgtcgga ggttgggggc tctgtggagg acctgattgc
     2401 caaaggcccc gtctcaaagt actcccaggc ggtgccagcc gtgaccgagg ggcccattcc
     2461 cgaggtgctc aagaactaca tggacgccca gtactacggg gagattggca tcgggacgcc
     2521 cccccagtgc ttcacagtcg tcttcgacac gggctcctcc aacctgtggg tcccctccat
     2581 ccactgcaaa ctgctggaca tcgcttgctg gatccaccac aagtacaaca gcgacaagtc
     2641 cagcacctac gtgaagaatg gtacctcgtt tgacatccac tatggctcgg gcagcctctc
     2701 cgggtacctg agccaggaca ctgtgtcggt gccctgccag tcagcgtcgt cagcctctgc
     2761 cctgggcggt gtcaaagtgg agaggcaggt ctttggggag gccaccaagc agccaggcat
     2821 caccttcatc gcagccaagt tcgatggcat cctgggcatg gcctaccccc gcatctccgt
     2881 caacaacgtg ctgcccgtct tcgacaacct gatgcagcag aagctggtgg accagaacat
     2941 cttctccttc tacctgagca gggacccaga tgcgcagcct gggggtgagc tgatgctggg
     3001 tggcacagac tccaagtatt acaagggttc tctgtcctac ctgaatgtca cccgcaaggc
     3061 ctactggcag gtccacctgg accaggtgga ggtggccagc gggctgaccc tgtgcaagga
     3121 gggctgtgag gccattgtgg acacaggcac ttccctcatg gtgggcccgg tggatgaggt
     3181 gcgcgagctg cagaaggcca tcggggccgt gccgctgatt cagggcgagt acatgatccc
     3241 ctgtgagaag gtgtccaccc tgcccgcgat cacactgaag ctgggaggca aaggctacaa
     3301 gctgtcccca gaggactaca cgctcaaggt gtcgcaggcc gggaagaccc tctgcctgag
     3361 cggcttcatg ggcatggaca tcccgccacc cagcgggcca ctctggatcc tgggcgacgt
     3421 cttcatcggc cgctactaca ctgtgtttga ccgtgacaac aacagggtgg gcttcgccga
     3481 ggctgcccgc ctcatcgata tggcaagcgc agaagactac gaatacccaa gcgaacagaa
     3541 gctcatctcc gaagaggatc tccaccatca tcaccaccat tgataattaa ttaacggatc
     3601 cggaattccg cccccccccc ccccccccct ctccctcccc cccccctaac gttactggcc
     3661 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
     3721 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
     3781 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
     3841 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
     3901 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
     3961 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
     4021 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
     4081 tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa
     4141 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataag
     4201 cttgccacaa cccacaagga gacgaccttc catgaccgag tacaagccca cggtgcgcct
     4261 cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt tcgccgacta
     4321 ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca ccgagctgca
     4381 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     4441 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     4501 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     4561 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     4621 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     4681 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     4741 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     4801 ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac
     4861 cgaaaggagc gcacgacccc atggctccga ccgaagccac ccggggcggc cccgccgacc
     4921 ccgcacccgc ccccgaggcc caccgactct agtcgagggc tgcagcgctg cagaggccga
     4981 gtgcagaact gctccaaagg gacctcaagg ctttccgagg gacactaggc tgactccatc
     5041 gagccagtgt agagataagc ttatcgatta gtccaatttg ttaaagacag gatatcagtg
     5101 gtccaggctc tagttttgac tcaacaatat caccagctga agcctataga gtacgagcca
     5161 tagataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc
     5221 tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa aaatacataa
     5281 ctgagaatag agaagttcag atcaaggtca ggaacagatg gaacagggtc gaccctagag
     5341 aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa
     5401 ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa
     5461 aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt cgcccgggta
     5521 cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg
     5581 ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatt tgggggctcg
     5641 tccgggatcg ggagacccct gcccagggac caccgaccca ccaccgggag gtaagctggc
     5701 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     5761 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     5821 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtag
     5881 atccggctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     5941 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     6001 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     6061 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     6121 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     6181 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tactggctta
     6241 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     6301 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
     6361 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     6421 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     6481 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6541 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6601 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6661 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6721 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6781 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6841 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6901 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     6961 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7021 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     7081 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     7141 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     7201 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     7261 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     7321 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     7381 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     7441 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     7501 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     7561 ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt
     7621 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     7681 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     7741 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     7801 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7861 tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca
     7921 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     7981 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     8041 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     8101 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     8161 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     8221 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     8281 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc
     8341 aagaattagc ttggccattg catacgttgt atccatatca taatatgtac atttatattg
     8401 gctcatgtcc aacattaccg ccatgttgac attgattatt gactagttat taatagtaat
     8461 caattacggg gtcattagtt catagcccat atatggagt
//
