LOCUS       sequence-358652-       13960 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  point mutation on psbA2 by CRISPR in Synechocystis 6803.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13960)
  AUTHORS   Ungerer J, Pakrasi HB
  TITLE     Cpf1 Is A Versatile Tool for CRISPR Genome Editing Across Diverse 
            Species of Cyanobacteria.
  JOURNAL   Sci Rep. 2016 Dec 21;6:39681. doi: 10.1038/srep39681.
  PUBMED    28000776
REFERENCE   2  (bases 1 to 13960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-12-21"; volume: "6:39681. doi"; pages: " 
            10.1038/srep39681"
COMMENT     Sequence Label: CRISPR-psbA2 point mutation
FEATURES             Location/Qualifiers
     source          1..13960
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(394..1209)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(394..1209)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1117..1136
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1201..1220
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      2067..2461
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            2803..2890
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             4129..5100
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             5611..6450
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             6437..7288
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     primer_bind     7798..7817
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        join(7921..7926,7927..7944,7945..7950)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        11874..11908
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
ORIGIN
        1 cattcatcag tgtaaaaatg gaatcaataa agccctgcgc agcgcgcagg gtcagcctga
       61 atacgcgttt aatgaccagc acagtcgtga tggcaaggtc agaatagcgc tgaggtctgc
      121 ctcgtgaaga aggtgttgct gactcatacc aggcctgaat cgccccatca tccagccaga
      181 aagtgaggga gccacggttg atgagagctt tgttgtaggt ggaccagttg gtgattttga
      241 acttttgctt tgccacggaa cggtctgcgt tgtcgggaag atgcgtgatc tgatccttca
      301 actcagcaaa agttcgattt attcaacaaa gccgccgtcc cgtcaagtca gcgtaatgct
      361 ctgccagtgt tacaaccaat taaccaattc tgattagaaa aactcatcga gcatcaaatg
      421 aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg
      481 taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc
      541 tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag
      601 gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagctt
      661 atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact
      721 cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc
      781 gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag
      841 cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt
      901 cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat
      961 ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc
     1021 attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata
     1081 caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata
     1141 taaatcagca tccatgttgg aatttaatcg cggcctcgag caagacgttt cccgttgaat
     1201 atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga
     1261 tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacaa cgtggctttg
     1321 ttgaataaat cgaacttttg ctgagttgaa ggatcagatc acgcatcttc ccgacaacgc
     1381 agaccgttcc gtggcaaagc aaaagttcaa aatcaccaac tggtccacct acaacaaagc
     1441 tctcatcaac cgtggctccc tcactttctg gctggatgat ggggcgattc aggcctggta
     1501 tgagtcagca acaccttctt cacgaggcag acctcagcgc tattctgacc ttgccatcac
     1561 gactgtgctg gtcattaaac gcgtattcag gctgaccctg cgcgctgcgc agggctttat
     1621 tgattccatt tttacactga tgaatgttcc gttgcgctgc ccggattaca gctgaaagcg
     1681 accaggtgct cggcgtggca agactcgcag cgaacccgta gaaagccatg ctccagccgc
     1741 ccgcattgga gaaattcttc aaattcccgt tgcacatagc ccggcaattc ctttccctgc
     1801 tctgccataa gcgcagcgaa tgccgggtaa tactcgtcaa cgatctgata gagaagggtt
     1861 tgctcgggtc ggtggctctg gtaacgacca gtatcccgat cccggctggc cgtcctggcc
     1921 gccacatgag gcatgttccg cgtccttgca atactgtgtt tacatacagt ctatcgctta
     1981 gcggaaagtt cttttaccct cagccgaaat gcctgccgtt gctagacatt gccagccagt
     2041 gcccgtcact cccgtactaa ctgtcacgaa cccctgcaat aactgtcacg cccccctgca
     2101 ataactgtca cgaacccctg caataactgt cacgccccca aacctgcaaa cccagcaggg
     2161 gcgggggctg gcggggtgtt ggaaaaatcc atccatgatt atctaagaat aatccactag
     2221 gcgcggttat cagcgccctt gtggggcgct gctgcccttg cccaatatgc ccggccagag
     2281 gccggatagc tggtctattc gctgcgctag gctacacacc gccccaccgc tgcgcggcag
     2341 ggggaaaggc gggcaaagcc cgctaaaccc cacaccaaac cccgcagaaa tacgctggag
     2401 cgcttttagc cgctttagcg gcctttcccc ctacccgaag ggtgggggcg cgtgtgcagc
     2461 cccgcagggc ctgtctcggt cgatcattca gcccggctca tccttctggc gtggcggcag
     2521 accgaacaag gcgcggtcgt ggtcgcgttc aaggtacgca tccattgccg ccatgagccg
     2581 atcctccggc cactcgctgc tgttcacctt ggccaaaatc atggccccca ccagcacctt
     2641 gcgccttgtt tcgttcttgc gctcttgctg ctgttccctt gcccgcaccc gctgaatttc
     2701 ggcattgatt cgcgctcgtt gttcttcgag cttggccagc cgatccgccg ccttgttgct
     2761 ccccttaacc atcttgacac cccattgtta atgtgctgtc tcgtaggcta tcatggaggc
     2821 acagcggcgg caatcccgac cctactttgt aggggagggc gcacttaccg gtttctcttc
     2881 gagaaactgg cctaacggcc acccttcggg cggtgcgctc tccgagggcc attgcatgga
     2941 gccgaaaagc aaaagcaaca gcgaggcagc atggcgattt atcaccttac ggcgaaaacc
     3001 ggcagcaggt cgggcggcca atcggccagg gccaaggccg actacatcca gcgcgaaggc
     3061 aagtatgccc gcgacatgga tgaagtcttg cacgccgaat ccgggcacat gccggagttc
     3121 gtcgagcggc ccgccgacta ctgggatgct gccgacctgt atgaacgcgc caatgggcgg
     3181 ctgttcaagg aggtcgaatt tgccctgccg gtcgagctga ccctcgacca gcagaaggcg
     3241 ctggcgtccg agttcgccca gcacctgacc ggtgccgagc gcctgccgta tacgctggcc
     3301 atccatgccg gtggcggcga gaacccgcac tgccacctga tgatctccga gcggatcaat
     3361 gacggcatcg agcggcccgc cgctcagtgg ttcaagcggt acaacggcaa gaccccggag
     3421 aagggcgggg cacagaagac cgaagcgctc aagcccaagg catggcttga gcagacccgc
     3481 gaggcatggg ccgaccatgc caaccgggca ttagagcggg ctggccacga cgcccgcatt
     3541 gaccacagaa cacttgaggc gcagggcatc gagcgcctgc ccggtgttca cctggggccg
     3601 aacgtggtgg agatggaagg ccggggcatc cgcaccgacc gggcagacgt ggccctgaac
     3661 atcgacaccg ccaacgccca gatcatcgac ttacaggaat accgggaggc aatagaccat
     3721 gaacgcaatc gacagagtga agaaatccag aggcatcaac gagttagcgg agcagatcga
     3781 accgctggcc cagagcatgg cgacactggc cgacgaagcc cggcaggtca tgagccagac
     3841 ccagcaggcc agcgaggcgc aggcggcgga gtggctgaaa gcccagcgcc agacaggggc
     3901 ggcatgggtg gagctggcca aagagttgcg ggaggtagcc gccgaggtga gcagcgccgc
     3961 gcagagcgcc cggagcgcgt cgcgggggtg gcactggaag ctatggctaa ccgtgatgct
     4021 ggcttccatg atgcctacgg tggtgctgct gatcgcatcg ttgctcttgc tcgacctgac
     4081 gccactgaca accgaggacg gctcgatctg gctgcgcttg gtggcccgat gaagaacgac
     4141 aggactttgc aggccatagg ccgacagctc aaggccatgg gctgtgagcg cttcgatatc
     4201 ggcgtcaggg acgccaccac cggccagatg atgaaccggg aatggtcagc cgccgaagtg
     4261 ctccagaaca cgccatggct caagcggatg aatgcccagg gcaatgacgt gtatatcagg
     4321 cccgccgagc aggagcggca tggtctggtg ctggtggacg acctcagcga gtttgacctg
     4381 gatgacatga aagccgaggg ccgggagcct gccctggtag tggaaaccag cccgaagaac
     4441 tatcaggcat gggtcaaggt ggccgacgcc gcaggcggtg aacttcgggg gcagattgcc
     4501 cggacgctgg ccagcgagta cgacgccgac ccggccagcg ccgacagccg ccactatggc
     4561 cgcttggcgg gcttcaccaa ccgcaaggac aagcacacca cccgcgccgg ttatcagccg
     4621 tgggtgctgc tgcgtgaatc caagggcaag accgccaccg ctggcccggc gctggtgcag
     4681 caggctggcc agcagatcga gcaggcccag cggcagcagg agaaggcccg caggctggcc
     4741 agcctcgaac tgcccgagcg gcagcttagc cgccaccggc gcacggcgct ggacgagtac
     4801 cgcagcgaga tggccgggct ggtcaagcgc ttcggtgatg acctcagcaa gtgcgacttt
     4861 atcgccgcgc agaagctggc cagccggggc cgcagtgccg aggaaatcgg caaggccatg
     4921 gccgaggcca gcccagcgct ggcagagcgc aagcccggcc acgaagcgga ttacatcgag
     4981 cgcaccgtca gcaaggtcat gggtctgccc agcgtccagc ttgcgcgggc cgagctggca
     5041 cgggcaccgg caccccgcca gcgaggcatg gacaggggcg ggccagattt cagcatgtag
     5101 tgcttgcgtt ggtactcacg cctgttatac tatgagtact cacgcacaga agggggtttt
     5161 atggaatacg aaaaaagcgc ttcagggtcg gtctacctga tcaaaagtga caagggctat
     5221 tggttgcccg gtggctttgg ttatacgtca aacaaggccg aggctggccg cttttcagtc
     5281 gctgatatgg ccagccttaa ccttgacggc tgcaccttgt ccttgttccg cgaagacaag
     5341 cctttcggcc ccggcaagtt tctcggtgac tgatatgaaa gaccaaaagg acaagcagac
     5401 cggcgacctg ctggccagcc ctgacgctgt acgccaagcg cgatatgccg agcgcatgaa
     5461 ggccaaaggg atgcgtcagc gcaagttctg gctgaccgac gacgaatacg aggcgctgcg
     5521 cgagtgcctg gaagaactca gagcggcgca gggcgggggt agtgaccccg ccagcgccta
     5581 accaccaact gcctgcaaag gaggcaatca atggctaccc ataagcctat caatattctg
     5641 gaggcgttcg cagcagcgcc gccaccgctg gactacgttt tgcccaacat ggtggccggt
     5701 acggtcgggg cgctggtgtc gcccggtggt gccggtaaat ccatgctggc cctgcaactg
     5761 gccgcacaga ttgcaggcgg gccggatctg ctggaggtgg gcgaactgcc caccggcccg
     5821 gtgatctacc tgcccgccga agacccgccc accgccattc atcaccgcct gcacgccctt
     5881 ggggcgcacc tcagcgccga ggaacggcaa gccgtggctg acggcctgct gatccagccg
     5941 ctgatcggca gcctgcccaa catcatggcc ccggagtggt tcgacggcct caagcgcgcc
     6001 gccgagggcc gccgcctgat ggtgctggac acgctgcgcc ggttccacat cgaggaagaa
     6061 aacgccagcg gccccatggc ccaggtcatc ggtcgcatgg aggccatcgc cgccgatacc
     6121 gggtgctcta tcgtgttcct gcaccatgcc agcaagggcg cggccatgat gggcgcaggc
     6181 gaccagcagc aggccagccg gggcagctcg gtactggtcg ataacatccg ctggcagtcc
     6241 tacctgtcga gcatgaccag cgccgaggcc gaggaatggg gtgtggacga cgaccagcgc
     6301 cggttcttcg tccgcttcgg tgtgagcaag gccaactatg gcgcaccgtt cgctgatcgg
     6361 tggttcaggc ggcatgacgg cggggtgctc aagcccgccg tgctggagag gcagcgcaag
     6421 agcaaggggg tgccccgtgg tgaagcctaa gaacaagcac agcctcagcc acgtccggca
     6481 cgacccggcg cactgtctgg cccccggcct gttccgtgcc ctcaagcggg gcgagcgcaa
     6541 gcgcagcaag ctggacgtga cgtatgacta cggcgacggc aagcggatcg agttcagcgg
     6601 cccggagccg ctgggcgctg atgatctgcg catcctgcaa gggctggtgg ccatggctgg
     6661 gcctaatggc ctagtgcttg gcccggaacc caagaccgaa ggcggacggc agctccggct
     6721 gttcctggaa cccaagtggg aggccgtcac cgctgatgcc atggtggtca aaggtagcta
     6781 tcgggcgctg gcaaaggaaa tcggggcaga ggtcgatagt ggtggggcgc tcaagcacat
     6841 acaggactgc atcgagcgcc tttggaaggt atccatcatc gcccagaatg gccgcaagcg
     6901 gcaggggttt cggctgctgt cggagtacgc cagcgacgag gcggacgggc gcctgtacgt
     6961 ggccctgaac cccttgatcg cgcaggccgt catgggtggc ggccagcatg tgcgcatcag
     7021 catggacgag gtgcgggcgc tggacagcga aaccgcccgc ctgctgcacc agcggctgtg
     7081 tggctggatc gaccccggca aaaccggcaa ggcttccata gataccttgt gcggctatgt
     7141 ctggccgtca gaggccagtg gttcgaccat gcgcaagcgc cgccagcggg tgcgcgaggc
     7201 gttgccggag ctggtcgcgc tgggctggac ggtaaccgag ttcgcggcgg gcaagtacga
     7261 catcacccgg cccaaggcgg caggctgacc ccccccactc tattgtaaac aagacatttt
     7321 tatcttttat attcaatggc ttattttcct gctaattggt aataccatga aaaataccat
     7381 gctcagaaaa ggcttaacaa tattttgaaa aattgcctac tgagcgctgc cgcacagctc
     7441 cataggccgc tttcctggct ttgcttccag atgtatgctc ttctgctcct gcaggcatcg
     7501 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     7561 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     7621 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     7681 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     7741 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata
     7801 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     7861 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     7921 tttacacttt atgcttccgg ctcgtatgtt aggaggtctt tatcatgtca atttatcaag
     7981 aatttgttaa taaatatagt ttaagtaaaa ctctaagatt tgagttaatc ccacagggta
     8041 aaacacttga aaacataaaa gcaagaggtt tgattttaga tgatgagaaa agagctaaag
     8101 actacaaaaa ggctaaacaa ataattgata aatatcatca gttttttata gaggagatat
     8161 taagttcggt ttgtattagc gaagatttat tacaaaacta ttctgatgtt tattttaaac
     8221 ttaaaaagag tgatgatgat aatctacaaa aagattttaa aagtgcaaaa gatacgataa
     8281 agaaacaaat atctgaatat ataaaggact cagagaaatt taagaatttg tttaatcaaa
     8341 accttatcga tgctaaaaaa gggcaagagt cagatttaat tctatggcta aagcaatcta
     8401 aggataatgg tatagaacta tttaaagcca atagtgatat cacagatata gatgaggcgt
     8461 tagaaataat caaatctttt aaaggttgga caacttattt taagggtttt catgaaaata
     8521 gaaaaaatgt ttatagtagc aatgatattc ctacatctat tatttatagg atagtagatg
     8581 ataatttgcc taaatttcta gaaaataaag ctaagtatga gagtttaaaa gacaaagctc
     8641 cagaagctat aaactatgaa caaattaaaa aagatttggc agaagagcta acctttgata
     8701 ttgactacaa aacatctgaa gttaatcaaa gagttttttc acttgatgaa gtttttgaga
     8761 tagcaaactt taataattat ctaaatcaaa gtggtattac taaatttaat actattattg
     8821 gtggtaaatt tgtaaatggt gaaaatacaa agagaaaagg tataaatgaa tatataaatc
     8881 tatactcaca gcaaataaat gataaaacac tcaaaaaata taaaatgagt gttttattta
     8941 agcaaatttt aagtgataca gaatctaaat cttttgtaat tgataagtta gaagatgata
     9001 gtgatgtagt tacaacgatg caaagttttt atgagcaaat agcagctttt aaaacagtag
     9061 aagaaaaatc tattaaagaa acactatctt tattatttga tgatttaaaa gctcaaaaac
     9121 ttgatttgag taaaatttat tttaaaaatg ataaatctct tactgatcta tcacaacaag
     9181 tttttgatga ttatagtgtt attggtacag cggtactaga atatataact caacaaatag
     9241 cacctaaaaa tcttgataac cctagtaaga aagagcaaga attaatagcc aaaaaaactg
     9301 aaaaagcaaa atacttatct ctagaaacta taaagcttgc cttagaagaa tttaataagc
     9361 atagagatat agataaacag tgtaggtttg aagaaatact tgcaaacttt gcggctattc
     9421 cgatgatatt tgatgaaata gctcaaaaca aagacaattt ggcacagata tctatcaaat
     9481 atcaaaatca aggtaaaaaa gacctacttc aagctagtgc ggaagatgat gttaaagcta
     9541 tcaaggatct tttagatcaa actaataatc tcttacataa actaaaaata tttcatatta
     9601 gtcagtcaga agataaggca aatattttag acaaggatga gcatttttat ctagtatttg
     9661 aggagtgcta ctttgagcta gcgaatatag tgcctcttta taacaaaatt agaaactata
     9721 taactcaaaa gccatatagt gatgagaaat ttaagctcaa ttttgagaac tcgactttgg
     9781 ctaatggttg ggataaaaat aaagagcctg acaatacggc aattttattt atcaaagatg
     9841 ataaatatta tctgggtgtg atgaataaga aaaataacaa aatatttgat gataaagcta
     9901 tcaaagaaaa taaaggcgag ggttataaaa aaattgttta taaactttta cctggcgcaa
     9961 ataaaatgtt acctaaggtt ttcttttctg ctaaatctat aaaattttat aatcctagtg
    10021 aagatatact tagaataaga aatcattcca cacatacaaa aaatggtagt cctcaaaaag
    10081 gatatgaaaa atttgagttt aatattgaag attgccgaaa atttatagat ttttataaac
    10141 agtctataag taagcatccg gagtggaaag attttggatt tagattttct gatactcaaa
    10201 gatataattc tatagatgaa ttttatagag aagttgaaaa tcaaggctac aaactaactt
    10261 ttgaaaatat atcagagagc tatattgata gcgtagttaa tcagggtaaa ttgtacctat
    10321 tccaaatcta taataaagat ttttcagctt atagcaaagg gcgaccaaat ctacatactt
    10381 tatattggaa agcgctgttt gatgagagaa atcttcaaga tgtggtttat aagctaaatg
    10441 gtgaggcaga gcttttttat cgtaaacaat caatacctaa aaaaatcact cacccagcta
    10501 aagaggcaat agctaataaa aacaaagata atcctaaaaa agagagtgtt tttgaatatg
    10561 atttaatcaa agataaacgc tttactgaag ataagttttt ctttcactgt cctattacaa
    10621 tcaattttaa atctagtgga gctaataagt ttaatgatga aatcaattta ttgctaaaag
    10681 aaaaagcaaa tgatgttcat atattaagta tagatagagg tgaaagacat ttagcttact
    10741 atactttggt agatggtaaa ggcaatatca tcaaacaaga tactttcaac atcattggta
    10801 atgatagaat gaaaacaaac taccatgata agcttgctgc aatagagaaa gatagggatt
    10861 cagctaggaa agactggaaa aagataaata acatcaaaga gatgaaagag ggctatctat
    10921 ctcaggtagt tcatgaaata gctaagctag ttatagagta taatgctatt gtggtttttg
    10981 aggatttaaa ttttggattt aaaagagggc gtttcaaggt agagaagcag gtctatcaaa
    11041 agttagaaaa aatgctaatt gagaaactaa actatctagt tttcaaagat aatgagtttg
    11101 ataaaactgg gggagtgctt agagcttatc agctaacagc accttttgag acttttaaaa
    11161 agatgggtaa acaaacaggt attatctact atgtaccagc tggttttact tcaaaaattt
    11221 gtcctgtaac tggttttgta aatcagttat atcctaagta tgaaagtgtc agcaaatctc
    11281 aagagttctt tagtaagttt gacaagattt gttataacct tgataagggc tattttgagt
    11341 ttagttttga ttataaaaac tttggtgaca aggctgccaa aggcaagtgg actatagcta
    11401 gctttgggag tagattgatt aactttagaa attcagataa aaatcataat tgggatactc
    11461 gagaagttta tccaactaaa gagttggaga aattgctaaa agattattct atcgaatatg
    11521 ggcatggcga atgtatcaaa gcagctattt gcggtgagag cgacaaaaag ttttttgcta
    11581 agctaactag tgtcctaaat actatcttac aaatgcgtaa ctcaaaaaca ggtactgagt
    11641 tagattatct aatttcacca gtagcagatg taaatggcaa tttctttgat tcgcgacagg
    11701 cgccaaaaaa tatgcctcaa gatgctgatg ccaatggtgc ttatcatatt gggctaaaag
    11761 gtctgatgct actaggtagg atcaaaaata atcaagaggg caaaaaactc aatttggtta
    11821 tcaaaaatga agagtatttt gagttcgtgc agaataggaa taactaagaa ttcttgacag
    11881 ctagctcagt cctaggtata atgctagcgc tgatttaggc aaaaacgggt ctaagaactt
    11941 taaataattt ctactgttgt agatcttcgg tcgcttgatc tttcgtctaa gaactttaaa
    12001 taatttctac tgttgtagat gctactattc ctgtgccttc agataattca gtctaagaac
    12061 tttaaataat ttctactgtt gtagatgtct agagcctttt gtattagtag ccggtctaag
    12121 aactttaaat aatttctact gttgtagatt agcgatttat gaaggtcatt tttttgtcta
    12181 gctttaatgc ggtagttggt accgagtggg taaccagcac cgacaaccgc atctacgtgg
    12241 gttggttcgg cgtgctgatg atccccactc tgctgaccgc caccatctgc ttcatcgttg
    12301 cgttcattgc agcccctccc gtcgacatcg acggcatccg tgagcccgtt gccggctctc
    12361 tcatgtatgg caacaacatc atttccggcg ctgttgttcc ttccagcaac gccatcggcc
    12421 tgcatttcta tccgatttgg gaagccgcta gcctcgacga gtggctgtac aacggtggtc
    12481 cttaccaatt agtggtcttc cacttcttgc tgggtatcag ctgctacatg ggtcgtcaat
    12541 gggagctgtc gtaccgcctc ggtatgcgcc cttggatctg tgttgcatac agtgctccac
    12601 tctcggctgc ttttgcagtg tttctgatct acccgatcgg ccaaggttcg ttctcggacg
    12661 gcatgcccct gggtatcagc ggcaccttca acttcatgtt cgtgttccaa gcagagcaca
    12721 acattttgat gcaccccttc cacatgctgg gtgtggctgg tgtgttcggt ggttcgctgt
    12781 tctcggcaat gcacggttcg ttggtgacca gctcgctggt gcgtgagacg accgagaccg
    12841 agagccaaaa ctacggctac aaatttggtc aagaggaaga gacctacaac atcgtggcag
    12901 cccacggata cttcggtcgc ttgatctttc aatacgcagc cttcaacaac agccgttcgc
    12961 tgcacttctt cctgggtgca tggccggtcg tgggcatctg gtttacctcc atgggcatca
    13021 gcaccatggc gttcaacctg aatggtttca acttcaacca gtcggttttg gatagccaag
    13081 gcaaagtgat caacacttgg gcagatgtgt tgaaccgtgc caacttgggc atggaagtga
    13141 tgcacgagcg taatgctcac aacttcccgc tcgacttggc agcaggcgaa gcgaccccgg
    13201 tcgctttgac tgcgccttca attcacggtt aattgccgga attacagcag tctgaataat
    13261 caaaaagcgc ctcgaatggg gcgctttttg atgcttggat attgattgta gacaaccttt
    13321 ggacttgcga tcgctaggga ttagagatcg tccagcagat cctcttcgtt gctgttttca
    13381 gtcggggttt gaatgccgaa aacggcagaa cccagcaagc tgacatccag ctccattcgt
    13441 tgctccattt gtcggagaaa atagcctgtc atcatagctg aggcgagtaa gccagctaaa
    13501 ttttcccgac tggttgcgat ttgaacattg aagccttcac tgggcaacat tcccagcaga
    13561 ccttgaacat tttgttgaat gatttcttgg atatctgaag tcgccgactt tgctacccgc
    13621 gccatcactt ctggtgactg atgctggagg tactgcaaca acgcattgcc cttaagggtt
    13681 tccgtgtcgc tgtggaaaaa gtcagaacta aagaccatta cggcggttca gaaacgtacc
    13741 caaactctat cgcatcacca ttaccacgtg ggtttggatc caagttcggt ttccagaact
    13801 tgctaggttc gttagtcgac ttctggtgct gctggagcag tggattcagc catcgcgttg
    13861 tctgtcgatt gcgatcgcgc gggaatcagg gcttctaagt tcatgccctc ggtggtaccg
    13921 tcgactctag aggatctgta atccgggcag cgcaacggaa
//
