LOCUS       sequence-358656-       10860 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for CALPAIN-7. Used for generating cell
            lines. F98D mutation. Has C-terminal mCherry tag. Dox-inducible. 
            Internal ID: WISP20-53..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10860)
  AUTHORS   Wenzel DM, Mackay DR, Skalicky JJ, Paine EL, Miller MS, Ullman KS, 
            Sundquist WI
  TITLE     Comprehensive analysis of the human ESCRT-III-MIT domain interactome
            reveals new cofactors for cytokinetic abscission.
  JOURNAL   Elife. 2022 Sep 15;11:e77779. doi: 10.7554/eLife.77779.
  PUBMED    36107470
REFERENCE   2  (bases 1 to 10860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-09-15"; volume: "11:e77779. doi"; pages: " 
            10.7554/eLife.77779"
COMMENT     Sequence Label: pLVX-CALPAIN-7-mCherry, F98D
FEATURES             Location/Qualifiers
     source          1..10860
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2206..2520
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2214..2232
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2250..2268
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2285..2303
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2321..2339
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2357..2375
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2392..2410
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2428..2446
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2493..2517
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2529..2538
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4974..5681
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(5116..5134)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5384..5403
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        5693..6192
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5728..5751)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6125..6144
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6213..6812
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6213..6232)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6709..6729
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6829..7417
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6882..6902)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7300..7311)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7624..8257
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8385..8401)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8385..8401)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8398..8420)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8409..8425
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8433..8439,8440..8457,8458..8463))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8478..8499
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8616..8633)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8787..9375)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8867..8886)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9546..10337,10338..10406))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10169..10188
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10407..10511)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10559..10693
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10596..10615)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10650..10669
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtt tactccctat
     2221 cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac gatgtcgagt
     2281 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
     2341 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
     2401 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
     2461 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     2521 tggagaaggc caccatggac gccacagcac tggagcggga cgctgtgcag ttcgcccgtc
     2581 tggcggttca gcgcgaccac gaaggccgct actccgaggc ggtgttttat tacaaggaag
     2641 ctgcacaagc cttaatttat gctgagatgg caggatcaag cctagaaaat attcaagaaa
     2701 aaataactga gtatctggaa agagttcaag ctctacattc agcagttcag tcaaagagtg
     2761 ctgatccttt gaagtcaaaa catcagttgg acttagagcg tgctcatttc cttgttacac
     2821 aagctgatga tgaagatgaa aaagagaatg ttgaagatgc tatagaattg tacacagaag
     2881 ctgtggatct ctgtctgaaa acatcttatg aaactgctga taaagtcctg caaaataaac
     2941 tgaaacagtt ggctcgacag gcgctagaca gagcagaagc gctgagtgag cctttgacca
     3001 agccagttgg caaaatcagt tcaacaagtg ttaagccaaa gccacctcca gtgagagcac
     3061 attttccact gggcgctaat cccttccttg aaagacctca gtcatttata agtcctcagt
     3121 catgtgatgc acaaggacag agatacacag cagaagaaat agaagtactc aggacaacat
     3181 caaaaataaa tggtatagaa tatgttcctt tcatgaatgt tgacctgaga gaacgttttg
     3241 cctatccaat gcctttctgt gatagatggg gcaagctacc attatcacct aaacaaaaaa
     3301 ctacattttc caagtgggta cgaccagaag acctcaccaa caatcctaca atgatatata
     3361 ctgtgtccag ttttagcata aagcagacaa tagtatcgga ttgctccttt gtggcatcac
     3421 tggccatcag tgcagcttat gaaagacgtt ttaataagaa gttaattacc ggcataattt
     3481 accctcaaaa caaggatggt gaaccagaat acaatccatg tgggaagtat atggtaaaac
     3541 ttcacctcaa tggtgtccca agaaaggtga taattgatga ccagttacct gttgatcaca
     3601 agggagaatt gctctgttct tattccaaca acaaaagtga attatgggtt tctctcatag
     3661 aaaaagcata catgaaagtc atgggaggat atgattttcc aggatccaac tccaatattg
     3721 atcttcatgc actgactggc tggataccag aaagaattgc tatgcattca gatagccaaa
     3781 ctttcagtaa ggataattct ttcagaatgc tttatcaaag atttcacaaa ggagatgtcc
     3841 tcatcactgc gtcaactgga atgatgacag aagctgaagg agagaagtgg ggtctggttc
     3901 ccacacacgc atatgctgtt ttggatatta gagagttcaa ggggctgcga tttatccagt
     3961 tgaaaaatcc ttggagtcat ttacgttgga aaggaagata cagtgaaaat gatgtaaaaa
     4021 actggactcc agagttgcaa aagtatttaa actttgatcc ccgaacagct cagaaaatag
     4081 acaacggaat attttggatt tcctgggatg atctctgcca gtattatgat gtgatttatt
     4141 tgagttggaa tccaggtctt tttaaagaat caacatgtat tcacagtact tgggatgcta
     4201 agcaaggacc tgtgaaagat gcctatagcc tggccaacaa cccccagtac aaactggagg
     4261 tgcagtgtcc acaggggggt gctgcagttt gggttttgct tagtagacac ataacagaca
     4321 aggatgattt tgcgaataat cgagaattta tcacaatggt tgtatacaag actgatggga
     4381 aaaaagttta ttacccagct gacccacctc catacattga tggaattcga attaacagcc
     4441 ctcattattt gactaagata aagctgacca cacctgggac gcacacgttc acgctagtgg
     4501 tttctcaata tgaaaaacag aacacaatcc attacacggt tcgggtatat tcagcatgca
     4561 gctttacttt ttcaaagatt ccttcaccat acaccttatc aaaacggatt aatggaaagt
     4621 ggagtggtca gagtgctgga ggatgtggaa atttccaaga gactcacaaa aataacccca
     4681 tctaccaatt ccatatagaa aagactgggc cgttactgat tgagctacga ggaccaaggc
     4741 aatatagcgt tggatttgag gttgtaacag tttctactct aggagatcct ggtccccatg
     4801 gctttctgag gaaatctagt ggtgactata ggtgtgggtt ttgctacctg gaattagaaa
     4861 atataccttc tgggatcttc aatatcattc ctagtacctt tttgcctaaa caagaaggac
     4921 cttttttctt ggactttaat agtattatcc ccatcaagat cacacaactt caggtgagca
     4981 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
     5041 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     5101 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
     5161 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
     5221 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     5281 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     5341 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     5401 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     5461 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     5521 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     5581 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     5641 gccgccactc caccggcggc atggacgagc tgtacaagtg atgaattcta ccgggtaggg
     5701 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg
     5761 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg
     5821 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc
     5881 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact
     5941 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc
     6001 ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc
     6061 gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc
     6121 ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct
     6181 ccgggccttt cgacctgcag cccaagctta ccatgaccga gtacaagccc acggtgcgcc
     6241 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     6301 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     6361 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     6421 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     6481 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     6541 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     6601 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     6661 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     6721 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     6781 cctggtgcat gacccgcaag cccggtgcct gacgggcgcg tctggaacaa tcaacctctg
     6841 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     6901 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     6961 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     7021 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     7081 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     7141 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     7201 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     7261 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     7321 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     7381 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattaattc tgcagtcgag
     7441 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     7501 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     7561 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaagaggg
     7621 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
     7681 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
     7741 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
     7801 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
     7861 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
     7921 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt
     7981 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
     8041 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
     8101 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     8161 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     8221 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     8281 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc ttgacattgc
     8341 tagcgtttac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     8401 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     8461 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     8521 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     8581 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     8641 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8701 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8761 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8821 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8881 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8941 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     9001 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     9061 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     9121 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     9181 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     9241 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     9301 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     9361 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     9421 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     9481 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     9541 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     9601 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     9661 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     9721 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9781 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9841 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9901 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9961 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    10021 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    10081 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    10141 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    10201 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    10261 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    10321 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    10381 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    10441 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    10501 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa
    10561 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
    10621 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
    10681 tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc
    10741 ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc
    10801 tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt
//
