LOCUS       sequence-358682-        9839 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid that directs the expression of NFE2L1/Nrf1 in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9839)
  AUTHORS   Forcina GC, Pope L, Murray M, Dong W, Abu-Remaileh M, Bertozzi CR, 
            Dixon SJ
  TITLE     Ferroptosis regulation by the NGLY1/NFE2L1 pathway.
  JOURNAL   Proc Natl Acad Sci U S A. 2022 Mar 15;119(11):e2118646119. doi: 
            10.1073/pnas.2118646119. Epub 2022 Mar 10.
  PUBMED    35271393
REFERENCE   2  (bases 1 to 9839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2022-03-15"; volume: "119(11):e2118646119. 
            doi"; pages: " 10.1073/pnas.2118646119. Epub 2022 Mar 10"
COMMENT     Sequence Label: pTwist-SFFV-NFE2L1-Puro
FEATURES             Location/Qualifiers
     source          1..9839
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2308..2334
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2308..2334
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    2414..2965
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2558..2575)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             2968..3567
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2968..2987)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3464..3484
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3592..4181
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3646..3666)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4064..4075)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4268..4501
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     rep_origin      4762..4897
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(5015..5603)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5095..5114)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(5965..6033,6034..6825)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6183..6202)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      6838..6974
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     enhancer        7051..7354
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7355..7558
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7509..7529
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7573..7753
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7797..7922
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8415..8648
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8833..8877
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9144..9261
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        9357..9758
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
ORIGIN
        1 atggactaca aagaccatga cggtgattat aaagatcatg atatcgatta caaggatgac
       61 gatgacaaga gatctgcggc cgctctttct ctgaagaaat acttaacgga aggacttctc
      121 cagttcacca ttctgctgag tttgattggg gtacgggtgg acgtggatac ttacctgacc
      181 tcacagcttc ccccactccg ggagatcatc ctggggccca gttctgccta tactcagacc
      241 cagttccaca acctgaggaa taccttggat ggctatggta tccaccccaa gagcatagac
      301 ctggacaatt acttcactgc ccggcggctc ctcagtcagg tgagggccct ggacaggttc
      361 caggtgccaa ccactgaggt aaatgcctgg ctggttcacc gagacccaga ggggtctgtc
      421 tctggcagtc agcccaactc aggcctcgcc ctcgagagtt ccagtggcct ccaagatgtg
      481 acaggcccag acaacggggt gcgagaaagc gaaacggagc agggattcgg tgaagatttg
      541 gaggatttgg gggctgtagc ccccccagtc agtggagact taaccaaaga ggacatagat
      601 ctgattgaca tcctttggcg acaggatatt gatctggggg ctgggcgtga ggtttttgac
      661 tatagtcacc gccagaagga gcaggatgtg gagaaggagc tgcgagatgg aggcgagcag
      721 gacacctggg caggcgaggg cgcggaagct ctggcacgga acctgctagt ggatggagag
      781 actggggaga gcttccctgc acagtttcca gcagacattt ccagcataac agaagcagtg
      841 cctagtgaga gtgagccccc tgctcttcaa aacaacctct tgtctcctct tctgaccggg
      901 acagagtcac catttgattt ggaacagcag tggcaagatc tcatgtccat catggaaatg
      961 caggccatgg aagtgaacac atcagcaagt gaaatcctgt acagtgcccc tcctggagac
     1021 ccactgagca ccaactacag ccttgccccc aacactccca tcaatcagaa tgtcagcctg
     1081 catcaggcgt ccctgggggg ctgcagccag gacttcttac tcttcagccc cgaggtggaa
     1141 agcctgcctg tggccagtag ctccacgctg ctcccgttgg cccccagcaa ttctaccagc
     1201 ctcaactcca ccttcggctc caccaacctg acagggctct tctttccacc ccagctcaat
     1261 ggcacagcca atgacacagc aggcccagag ctgcctgacc ctttgggggg tctgttagat
     1321 gaagctatgt tggatgagat cagccttatg gacctggcca ttgaagaagg ctttaaccct
     1381 gtgcaggcct cccagctgga ggaggaattt gactctgact caggcctttc cttagactcg
     1441 agccatagcc cttcttccct aagcagctct gaaggcagtt cttcctcttc ttcctcctcc
     1501 tcttcctctt cttcctctgc ttcttcctct gcctcttcct ccttttctga ggaaggtgcg
     1561 gttggctaca gctctgactc tgagaccctg gatctggaag aggccgaggg tgctgtgggc
     1621 taccagcctg agtattccaa gttctgccgc atgagctacc aggatccagc tcagctctca
     1681 tgcctgccct acctggagca cgtgggccac aaccacacat acaacatggc acccagtgcc
     1741 ctggactcag ccgacctgcc accacccagt gccctcaaga aaggcagcaa ggagaagcag
     1801 gctgacttcc tggacaagca gatgagccgg gatgagcacc gagcccgagc catgaagatc
     1861 cctttcacca atgacaaaat catcaacctg cctgtggagg agttcaatga actgctgtcc
     1921 aaataccagt tgagtgaagc ccagctgagc ctcatccgag acatccggcg ccggggcaag
     1981 aacaagatgg cggcgcagaa ctgccgcaag cgcaagctgg acaccatcct gaatctggag
     2041 cgtgatgtgg aggacctgca gcgtgacaaa gcccggctgc tgcgggagaa agtggagttc
     2101 ctgcgctccc tgcgacagat gaagcagaag gtccagagcc tgtaccagga ggtgtttggg
     2161 cggctgcgag atgagaacgg acgaccctac tcgcccagtc agtatgcgct ccagtacgcc
     2221 ggggacggca gtgtcctcct catcccccgc acgatggccg accagcaggc ccggcggcag
     2281 gagaggaagc caaaggaccg gagaaagtac ccctacgacg tgcccgacta cgcctgactc
     2341 gagcttattc cagatgcgtg cggatggaat tcgagctcgg taccatgcca aaagcaaagc
     2401 gctatcgcgc cttacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
     2461 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
     2521 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
     2581 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
     2641 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
     2701 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
     2761 tggatagttg tggaaagagt caaatggctc ccctcaagcg tattcaacaa ggggctgaag
     2821 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
     2881 tcatgtgttt agtcgaggtt aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
     2941 tcctttgaaa aacacgatga taataacatg accgagtaca agcccacggt gcgcctcgcc
     3001 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     3061 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     3121 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     3181 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     3241 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggagggc
     3301 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     3361 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggccgcc
     3421 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     3481 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     3541 tgcatgaccc gcaagcccgg tgcctaggct agcttgactg actgagtcga caatcaacct
     3601 tttggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     3661 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     3721 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     3781 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     3841 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     3901 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     3961 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     4021 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     4081 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     4141 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc gagctcggta
     4201 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     4261 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     4321 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     4381 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     4441 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     4501 agtcctggcc aacgtgagca ccgtgctgac ctccaaatat cgttaagctg gagcctggga
     4561 gccggcctgg ccctccgccc cccccacccc cgcagcccac ccctggtctt tgaataaagt
     4621 ctgagtgagt ggccgacagt gcccgtggag ttctcgtgac ctgaggtgca gggccggcgc
     4681 tagggacacg tccgtgcacg tgccgaggcc ccctgtgcag ctgcaaggga caggcctagc
     4741 cctgcaggcc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     4801 cctcatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     4861 ctattccaga agtagtgagg acgctttttt ggaggccgag gcttttgcaa agatcgaaca
     4921 agagacagga cctgcaggtt aattaaattt aaatcatgtg agcaaaaggc cagcaaaagg
     4981 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     5041 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     5101 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     5161 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     5221 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     5281 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     5341 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     5401 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     5461 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     5521 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     5581 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     5641 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     5701 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     5761 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     5821 ttcgttcatc catagttgca tttaaatggc cggcctggcg cgccgtttaa acctagatat
     5881 tgatagtctg atcggtcaac gtataatcga gtcctagctt ttgcaaacat ctatcaagag
     5941 acaggatcag caggaggctt tcgcatgagt attcaacatt tccgtgtcgc ccttattccc
     6001 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     6061 gatgctgaag atcagttggg tgcgcgagtg ggttacatcg aactggatct caacagcggt
     6121 aagatccttg agagttttcg ccccgaagaa cgctttccaa tgatgagcac ttttaaagtt
     6181 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     6241 atacactatt ctcagaatga cttggttgag tattcaccag tcacagaaaa gcatcttacg
     6301 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     6361 gccaacttac ttctgacaac gattggagga ccgaaggagc taaccgcttt tttgcacaac
     6421 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     6481 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caaccttgcg taaactatta
     6541 actggcgaac tacttactct agcttcccgg caacagttga tagactggat ggaggcggat
     6601 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     6661 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     6721 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     6781 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaaccgat tctaggtgca
     6841 ttggcgcaga aaaaaatgcc tgatgcgacg ctgcgcgtct tatactccca catatgccag
     6901 attcagcaac ggatacggct tccccaactt gcccacttcc atacgtgtcc tccttaccag
     6961 aaatttatcc ttaagatccc gaatcgttta aacgcgatcg cagtaatcaa ttacggggtc
     7021 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     7081 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     7141 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     7201 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     7261 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     7321 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     7381 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     7441 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     7501 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
     7561 tttagtgaac cggggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     7621 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     7681 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     7741 aaaatctcta gcagtggcgc ccgaacaggg acctgaaagc gaaagggaaa ccagagctct
     7801 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     7861 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     7921 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     7981 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     8041 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     8101 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     8161 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     8221 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct
     8281 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     8341 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     8401 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     8461 ggcgcagcct caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     8521 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     8581 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     8641 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     8701 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     8761 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     8821 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     8881 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     8941 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     9001 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     9061 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     9121 ggatctcgac ggtatcggtt aacttttaaa agaaaagggg ggattggggg gtacagtgca
     9181 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     9241 attacaaaaa ttcaaaattt tggctcccga tcgttgcgtt acacacacaa ttactgctga
     9301 tcgagtgtag ccttcgaatg aaagacccca cctgtaggtt tggcaagata gctgcagtaa
     9361 cgccattttg caaggcatgg aaaaatacca aaccaagaat agagaagttc agatcaaggg
     9421 cgggtacatg aaaatagcta acgttgggcc aaacaggata tctgcggtga gcagtttcgg
     9481 ccccggcccg gggccaagaa cagatggtca ccgcagtttc ggccccggcc cgaggccaag
     9541 aacagatggt ccccagatat ggcccaaccc tcagcagttt cttaagaccc atcagatgtt
     9601 tccaggctcc cccaaggacc tgaaatgacc ctgcgcctta tttgaattaa ccaatcagcc
     9661 tgcttctcgc ttctgttcgc gcgcttctgc ttcccgagct ctataaaaga gctcacaacc
     9721 cctcactcgg cgcgccagtc ctccgattga ctgagtcgcc ctgatcattg tcgatcctac
     9781 catccactcg acacacccgc cagggcccgc atccaccatc gcagacttat catggatcc
//
