LOCUS       sequence-358692-        9788 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid that directs the expression of NFE2L2/Nrf2 in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9788)
  AUTHORS   Forcina GC, Pope L, Murray M, Dong W, Abu-Remaileh M, Bertozzi CR, 
            Dixon SJ
  TITLE     Ferroptosis regulation by the NGLY1/NFE2L1 pathway.
  JOURNAL   Proc Natl Acad Sci U S A. 2022 Mar 15;119(11):e2118646119. doi: 
            10.1073/pnas.2118646119. Epub 2022 Mar 10.
  PUBMED    35271393
REFERENCE   2  (bases 1 to 9788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2022-03-15"; volume: "119(11):e2118646119. 
            doi"; pages: " 10.1073/pnas.2118646119. Epub 2022 Mar 10"
COMMENT     Sequence Label: pLenti-CMV-NFE2L2-Puro
FEATURES             Location/Qualifiers
     source          1..9788
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..42
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     276..293
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    410..431
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(446..451,452..469,470..476)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    484..500
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     489..511
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     508..524
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     508..524
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     543..563
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        545..563
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        591..817
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             818..998
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1045..1170
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1663..1896
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2081..2125
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2392..2508
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2531..2834
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2835..3038
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2989..3009
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3035..3059
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3143..3167
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4983..5007)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5038..5626
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5091..5111)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5509..5520)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5640..6139
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5675..5698)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6072..6091
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6160..6759
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6160..6179)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6656..6676
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6897..7130
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7202..7323
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7239..7258)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7293..7312
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7363..7498
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7518..7537)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7519..7537)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7547..7564)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7547..7563)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7556..7578)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7705..8160
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7792..7811)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8002..8023
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8186..8290
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8291..8359,8360..9151)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8509..8528)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9322..9788,1..122)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
       61 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      121 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      181 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      241 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      301 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      361 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      421 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      481 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
      541 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
      601 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
      661 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
      721 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
      781 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
      841 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      901 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      961 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     1021 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     1081 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     1141 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     1201 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     1261 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     1321 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     1381 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     1441 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     1501 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1561 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1621 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1681 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1741 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1801 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1861 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1921 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1981 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2041 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2101 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2161 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2221 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2281 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2341 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag
     2401 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     2461 catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat cgataagctt
     2521 gggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2581 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2641 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2701 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2761 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2821 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2881 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2941 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     3001 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     3061 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgactc tagtccagtg
     3121 tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gttggcatga tggacttgga
     3181 gctgccgccg ccgggactcc cgtcccagca ggacatggat ttgattgaca tactttggag
     3241 gcaagatata gatcttggag taagtcgaga agtatttgac ttcagtcagc gacggaaaga
     3301 gtatgagctg gaaaaacaga aaaaacttga aaaggaaaga caagaacaac tccaaaagga
     3361 gcaagagaaa gcctttttcg ctcagttaca actagatgaa gagacaggtg aatttctccc
     3421 aattcagcca gcccagcaca tccagtcaga aaccagtgga tctgccaact actcccaggt
     3481 tgcccacatt cccaaatcag atgctttgta ctttgatgac tgcatgcagc ttttggcgca
     3541 gacattcccg tttgtagatg acaatgaggt ttcttcggct acgtttcagt cacttgttcc
     3601 tgatattccc ggtcacatcg agagcccagt cttcattgct actaatcagg ctcagtcacc
     3661 tgaaacttct gttgctcagg tagcccctgt tgatttagac ggtatgcaac aggacattga
     3721 gcaagtttgg gaggagctat tatccattcc tgagttacag tgtcttaata ttgaaaatga
     3781 caagctggtt gagactacca tggttccaag tccagaagcc aaactgacag aagttgacaa
     3841 ttatcatttt tactcatcta taccctcaat ggaaaaagaa gtaggtaact gtagtccaca
     3901 ttttcttaat gcttttgagg attccttcag cagcatcctc tccacagaag accccaacca
     3961 gttgacagtg aactcattaa attcagatgc cacagtcaac acagattttg gtgatgaatt
     4021 ttattctgct ttcatagctg agcccagtat cagcaacagc atgccctcac ctgctacttt
     4081 aagccattca ctctctgaac ttctaaatgg gcccattgat gtttctgatc tatcactttg
     4141 caaagctttc aaccaaaacc accctgaaag cacagcagaa ttcaatgatt ctgactccgg
     4201 catttcacta aacacaagtc ccagtgtggc atcaccagaa cactcagtgg aatcttccag
     4261 ctatggagac acactacttg gcctcagtga ttctgaagtg gaagagctag atagtgcccc
     4321 tggaagtgtc aaacagaatg gtcctaaaac accagtacat tcttctgggg atatggtaca
     4381 acccttgtca ccatctcagg ggcagagcac tcacgtgcat gatgcccaat gtgagaacac
     4441 accagagaaa gaattgcctg taagtcctgg tcatcggaaa accccattca caaaagacaa
     4501 acattcaagc cgcttggagg ctcatctcac aagagatgaa cttagggcaa aagctctcca
     4561 tatcccattc cctgtagaaa aaatcattaa cctccctgtt gttgacttca acgaaatgat
     4621 gtccaaagag cagttcaatg aagctcaact tgcattaatt cgggatatac gtaggagggg
     4681 taagaataaa gtggctgctc agaattgcag aaaaagaaaa ctggaaaata tagtagaact
     4741 agagcaagat ttagatcatt tgaaagatga aaaagaaaaa ttgctcaaag aaaaaggaga
     4801 aaatgacaaa agccttcacc tactgaaaaa acaactcagc accttatatc tcgaagtttt
     4861 cagcatgcta cgtgatgaag atggaaaacc ttattctcct agtgaatact ccctgcagca
     4921 aacaagagat ggcaatgttt tccttgttcc caaaagtaag aagccagatg ttaagaaaaa
     4981 ctacccaact ttcttgtaca aagtggttga tatccagcac agtggcggcc gctcgacaat
     5041 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     5101 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     5161 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     5221 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     5281 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     5341 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     5401 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     5461 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     5521 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     5581 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattctaccg
     5641 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
     5701 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
     5761 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
     5821 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
     5881 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
     5941 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
     6001 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
     6061 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
     6121 ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta caagcccacg
     6181 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     6241 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     6301 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     6361 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     6421 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     6481 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     6541 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     6601 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     6661 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     6721 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag
     6781 cgcccgaccg aaaggagcgc acgaccccat gcatctcgag ggcccggtac ctttaagacc
     6841 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     6901 agggctagct cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     6961 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7021 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7081 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     7141 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     7201 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     7261 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     7321 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     7381 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     7441 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     7501 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     7561 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     7621 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     7681 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     7741 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     7801 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     7861 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     7921 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     7981 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     8041 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     8101 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     8161 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     8221 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     8281 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     8341 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     8401 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     8461 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     8521 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     8581 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     8641 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     8701 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     8761 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     8821 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     8881 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     8941 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     9001 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     9061 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     9121 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     9181 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     9241 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     9301 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     9361 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     9421 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     9481 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     9541 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     9601 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     9661 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     9721 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     9781 aacaggag
//
