LOCUS       sequence-35887-e       12020 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12020)
  AUTHORS   Yoo AS, Sun AX, Li L, Shcheglovitov A, Portmann T, Li Y, Lee-Messer 
            C, Dolmetsch RE, Tsien RW, Crabtree GR
  TITLE     MicroRNA-mediated conversion of human fibroblasts to neurons.
  JOURNAL   Nature. 2011 Jul 13. doi: 10.1038/nature10323.
  PUBMED    21753754
REFERENCE   2  (bases 1 to 12020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2011 Jul 13. doi: 10.1038/nature10323."
COMMENT     Sequence Label: phMYT1L-N106
FEATURES             Location/Qualifiers
     source          1..12020
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2221..2432
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2433..3371
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3328..3348
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3393..3402
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        6994..7493
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7029..7052)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7426..7445
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7520..7918
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    7942..8530
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7995..8015)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8413..8424)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8738..9371
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9500..9516)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9500..9516)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9513..9535)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9524..9540
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9548..9554,9555..9572,9573..9578))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9593..9614
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9731..9748)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9902..10487)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9982..10001)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10658..11449,11450..11518))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11281..11300
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11519..11623)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11671..11805
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11708..11727)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11762..11781
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccgcgg ccgccaccat ggaggtggac accgaggaga
     3421 agcggcatcg cacgcggtcc aaaggggttc gagttcccgt ggaaccagcc atacaagagc
     3481 tgttcagctg tcccacccct ggctgtgacg gcagtggtca tgtcagtggc aaatatgcaa
     3541 gacacagaag tgtatatggt tgtcccttgg cgaaaaaaag aaaaacacaa gataaacagc
     3601 cccaggaacc tgctcctaaa cgaaagccat ttgccgtgaa agcagacagc tcctcagtgg
     3661 atgagtgtga cgacagtgat gggactgagg acatggatga gaaggaggag gatgaggggg
     3721 aggagtactc cgaggacaat gacgagccag gggatgagga cgaggaggac gaggaggggg
     3781 accgggagga ggaggaggag atcgaggagg aggatgagga cgatgacgag gatggagaag
     3841 atgtggagga tgaagaagag gaagaggagg aggaggagga ggaggaagag gaagaagaaa
     3901 acgaagacca tcaaatgaat tgtcacaata ctcgaataat gcaagacaca gaaaaggatg
     3961 ataacaataa tgacgaatat gacaattacg atgaactggt ggccaagtca ttgttaaacc
     4021 tcggcaaaat cgctgaggat gcagcctacc gggccaggac tgagtcagaa atgaacagca
     4081 atacctccaa tagtctggaa gacgatagtg acaaaaacga aaacctgggt cggaaaagtg
     4141 agttgagttt agacttagac agtgatgttg ttagagaaac agtggactcc cttaaactat
     4201 tagcccaagg acacggtgtt gtgctctcag aaaacatgaa tgacagaaat tatgcagaca
     4261 gcatgtcgca gcaagacagt agaaatatga attacgtcat gttggggaag cccatgaaca
     4321 atggactcat ggaaaagatg gtggaggaga gcgatgagga ggtgtgtctg agcagtctgg
     4381 agtgtttgag gaatcagtgc ttcgacctgg ccaggaagct cagtgagacc aacccgcagg
     4441 agaggaatcc gcagcagaac atgaacatcc gtcagcatgt ccggccagaa gaggacttcc
     4501 caggaaggac gccggacaga aactactcgg acatgctgaa cctcatgcgg ctggaggagc
     4561 agttgagccc ccggtcgaga gtgtttgcca gctgtgcgaa ggaggatggg tgtcatgagc
     4621 gggacgacga taccacctct gtgaactcgg acaggtctga agaggtgttc gacatgacca
     4681 aggggaacct gaccctgctg gagaaagcca tcgctttgga aacggaaaga gcaaaggcca
     4741 tgagggagaa gatggccatg gaagctggga ggagggacaa tatgaggtca tatgaggacc
     4801 agtctccgag acaacttccc ggggaggaca gaaagcctaa atccagtgac agccatgtca
     4861 aaaagccata ctatgatccc tcaagaacag aaaagaaaga gagcaagtgt ccaacccccg
     4921 ggtgtgatgg aaccggccac gtaactgggc tgtacccaca tcaccgcagc ctgtccggat
     4981 gcccgcacaa agatagggtc cctccagaaa tccttgccat gcatgaaagt gtcctcaagt
     5041 gccccactcc gggctgcacg gggcgcgggc atgtcaacag caacaggaac tcccaccgaa
     5101 gcctctccgg atgcccgatc gctgcagcag agaaactggc caaggcacag gaaaagcacc
     5161 agagctgcga cgtgtccaag tccagccagg cctcggaccg cgtgctcagg ccaatgtgct
     5221 ttgtgaagca gctggagatt cctcagtatg gctacagaaa caatgtcccc acaactacgc
     5281 cgcgttccaa cctggccaag gagctcgaga aatattccaa gacctcgttt gaatacaaca
     5341 gttacgacaa ccatacttat ggcaagcgag ccatagctcc caaggtgcaa accagggata
     5401 tatcccccaa aggatatgat gatgcgaagc ggtactgcaa ggaccccagc cccagcagca
     5461 gcagcaccag cagctacgcg cccagcagca gcagcaacct gagctgcggc gggggcagca
     5521 gcgccagcag cacgtgcagc aagagcagct tcgactacac gcacgacatg gaggcggccc
     5581 acatggcggc caccgccatc ctcaacctgt ccacgcgctg ccgcgagatg ccgcagaacc
     5641 tgagcaccaa gccgcaggac ctgtgcgcca cgcggaaccc tgacatggag gtggatgaga
     5701 acgggaccct ggacctcagc atgaacaagc agaggccgcg ggacagctgc tgccccatcc
     5761 tgacccctct ggagcccatg tccccccagc agcaggcagt gatgaacaac cggtgtttcc
     5821 agctgggcga gggcgactgc tgggacttgc ccgtagacta caccaaaatg aaaccccgga
     5881 ggatagacga ggacgagtcc aaagacatta ctccagaaga cttggaccca ttccaggagg
     5941 ctctagaaga aagacggtat cccggggagg tgaccatccc aagtcccaaa cccaagtacc
     6001 ctcagtgcaa ggagagcaaa aaggacttaa taactctgtc tggctgcccc ctggcggaca
     6061 aaagcattcg aagtatgctg gccaccagct cccaagaact caagtgcccc acgcctggct
     6121 gtgatggttc tggacatatc accggcaatt atgcttctca tcggagcctt tcaggttgcc
     6181 caagagcaaa gaaaagtggt atcaggatag cacagagcaa agaagataaa gaagatcaag
     6241 aacccatcag gtgtccggtc cccgggtgcg acggccaggg ccacatcact gggaagtacg
     6301 cgtcccatcg cagcgcctcc gggtgcccct tggcggccaa gaggcagaaa gacgggtacc
     6361 tgaatggctc ccagttctcc tggaagtcgg tcaagacgga aggcatgtcc tgccccacgc
     6421 caggatgcga cggctcaggc cacgtcagcg gcagcttcct cacacaccgc agcttgtcag
     6481 gatgcccgag agccacgtca gcgatgaaga aggcaaagct ttctggagag cagatgctga
     6541 ccatcaaaca gcgggccagc aacggtatag aaaatgatga agaaatcaaa cagttagatg
     6601 aagaaatcaa ggagctaaat gaatccaatt cccagatgga agccgatatg attaaactca
     6661 gaactcagat taccacgatg gagagcaacc tgaagaccat cgaagaggag aacaaagtga
     6721 ttgagcagca gaacgagtct ctcctccacg agctggcgaa cctgagccag tctctgatcc
     6781 acagcctggc taacatccag ctgccgcaca tggatccaat caatgaacaa aattttgatg
     6841 cttacgtgac tactttgacg gaaatgtata caaatcaaga tcgttatcag agtccagaaa
     6901 ataaagccct actggaaaat ataaagcagg ctgtgagagg aattcaggtc tgaggccgcg
     6961 ccggctctag atcgcgaacg cgtgaattct accgggtagg ggaggcgctt ttcccaaggc
     7021 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct
     7081 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
     7141 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
     7201 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
     7261 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
     7321 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
     7381 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc
     7441 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca
     7501 gcccaagctt cctaggacca tggccaagcc tttgtctcaa gaagaatcca ccctcattga
     7561 aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc
     7621 agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg
     7681 accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac
     7741 ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg
     7801 acaggtgctt ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca
     7861 gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg agggctaacc
     7921 cgggcggggc gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact
     7981 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     8041 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     8101 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     8161 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     8221 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     8281 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     8341 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     8401 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     8461 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     8521 gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga gcaatcacaa
     8581 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     8641 aggtgggttt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct
     8701 gtagatctta gccacttttt aaaagaaaag aggggactgg aagggctaat tcactcccaa
     8761 cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattag
     8821 cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag
     8881 ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg
     8941 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg
     9001 tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag
     9061 aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt
     9121 ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt
     9181 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     9241 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     9301 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     9361 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     9421 atgaatatca gagagtgaga ggccttgaca ttgctagcgt tttaccgtcg acctctagct
     9481 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     9541 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     9601 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     9661 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     9721 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     9781 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     9841 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     9901 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     9961 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
    10021 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
    10081 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
    10141 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
    10201 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
    10261 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    10321 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
    10381 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtt
    10441 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
    10501 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
    10561 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
    10621 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
    10681 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    10741 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    10801 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    10861 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    10921 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    10981 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    11041 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    11101 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    11161 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    11221 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    11281 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    11341 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    11401 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    11461 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    11521 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    11581 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    11641 tgccacctga cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac
    11701 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
    11761 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
    11821 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattaccta
    11881 gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg
    11941 tatttgttaa atatgtacta caaacttagt agtttttaaa gaaattgtat ttgttaaata
    12001 tgtactacaa acttagtagt
//
