LOCUS       sequence-35898-e         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral transfer vector expressing shp53 under an 
            hUbiquitin promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P
  TITLE     A versatile tool for conditional gene expression and knockdown.
  JOURNAL   Nat Methods. 2006 Feb;3(2):109-16. doi: 10.1038/nmeth846
  PUBMED    16432520
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Methods"; date: "2006-02"; volume: "3"; issue: "2"; pages: 
            "109-16"
COMMENT     Sequence Label: pLVUHshp53
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(130..163)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             183..363
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(387..404)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    393..617
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(750..769)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctttggattt gggatcttat aagttctgta tgagaccaca gatccccgac tccagtggta
       61 atctacttca agagagtaga ttaccactgg agtctttttg gaaaagctta tcgataccgt
      121 cgcatgggaa taacttcgta tagcatacat tatacgaagt tatgctgctt tttgcttgta
      181 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
      241 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
      301 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
      361 gcagcatcta gaaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
      421 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
      481 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
      541 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
      601 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
      661 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
      721 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      781 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      841 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      901 gccatcgccc tgatagacgg tttttcgccc tttgacg
//
