LOCUS       sequence-359004-         625 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Ef1a driven PE2 with pegRNA for editing C201R mutation (including 
            silent PAM mutation) in KCNQ2. See Addgene plasmid #184445.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 625)
  TITLE     All in one plasmids SCN1A and KCNQ2 mutations
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAIO-Ef1a-PE2-GFP:KCNQ2-C201R_pp
FEATURES             Location/Qualifiers
     source          1..625
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     84..103
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        182..257
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
       61 ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg
      121 atttcttggc tttatatatc ttgtggaaag gacgaaacac cgcagaatct gcaggaagca
      181 cgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag
      241 tggcaccgag tcggtgctcc ggagtctgcg cttcctgcag atttttttta gagccacctg
      301 cgggcagatc gaacctgcag gttccactgg gacgattaca tgcatctgga tcgatgagta
      361 attcatacaa aaggactcgc ccctgccttg gggaatccca gggaccgtcg ttaaactccc
      421 actaacgtag aacccagaga tcgctgcgtt cccgccccct cacccgcccg ctctcgtcat
      481 cactgaggtg gagaagagca tgcgtgaggc tccggtgccc gtcagtgggc agagcgcaca
      541 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga
      601 aggtggcgcg gggtaaactg ggaaa
//
