LOCUS       sequence-359008-         432 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For the mammalian expression of the tardigrade protein 
            SAHS1_A0A1D1UJR2. This is one of the bottom performing targets in 
            our apoptosis assay (it induced apoptosis)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Veling MT, Nguyen DT, Thadani NN, Oster ME, Rollins NJ, Brock KP, 
            Bethel NP, Lim S, Baker D, Way JC, Marks DS, Chang RL, Silver PA
  TITLE     Natural and Designed Proteins Inspired by Extremotolerant Organisms 
            Can Form Condensates and Attenuate Apoptosis in Human Cells.
  JOURNAL   ACS Synth Biol. 2022 Mar 18;11(3):1292-1302. doi: 
            10.1021/acssynbio.1c00572. Epub 2022 Feb 18.
  PUBMED    35176859
REFERENCE   2  (bases 1 to 432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00572"; journalName: "ACS Synth Biol"; date: 
            "2022-03-18- 18"; volume: "11"; issue: "3"; pages: "1292-1302"
COMMENT     Sequence Label: SAHS10_A0A1D1UJR2_RAMVA pSecTag (pBS0250)
FEATURES             Location/Qualifiers
     source          1..432
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atggattaca aggatgatga tgataagatg atgggtatga gaagcgccga catccccctg
       61 agccaccccc acctgaagca caacaacaga gtgtgggtga acacctacaa gaagggcgac
      121 gagtaccacc acaagatcat catcaaggag gccggctaca ccaacgacgt ggtgttcaag
      181 ctgggccagg agagcgccgg cagccacaac ggcagcagct tcagcctgaa gtacgaggac
      241 aaggacggcg ccctggtggg caccgtgcac agaaccggca ccaaggagca gcccctggac
      301 aagaccatca acaacgtgtt caagctggag ggcgaccacc ttgtgatgac cagcaccatc
      361 gacggcgtga ctatgaagag atactacaag accagaacct gagggtgttt gtggttttgt
      421 tgtgtggtat tg
//
