LOCUS       sequence-35926-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  TITLE     Other Lawson Lab plasmids
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 474 p5E-basprom
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    24..119
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    185..308
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(380..399)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(381..399)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(404..420)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(404..420)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(522..541)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(600..619)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gagttacgct accatggagc tccaaataat gattttattt tgactgatag tgacctgttc
       61 gttgcaacaa attgataagc aatgcttttt tataatgcca actttgtata gaaaagttgt
      121 acgtactaga gggtatataa tggatcccat cgcgtctcag cctcactttg agctcctcca
      181 cacgcaagtt tgtacaaaaa agttgaacga gaaacgtaaa atgatataaa tatcaatata
      241 ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca acatatgcag
      301 tcactatgaa ccaactactt agatggtatt agtgacctgt agaattcgag ctctagagct
      361 gcagggcggc cgcgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg
      421 gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
      481 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca
      541 tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg
      601 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg
      661 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
      721 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
      781 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac
      841 agcattcc
//
