LOCUS       sequence-35943-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Yang W, Paschen W
  TITLE     Gene expression and cell growth are modified by silencing SUMO2 and 
            SUMO3 expression.
  JOURNAL   Biochem Biophys Res Commun. 2009 Apr 24. 382(1):215-8.
  PUBMED    19275883
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem 
            Biophys Res Commun. 2009 Apr 24. 382(1):215-8."
COMMENT     Sequence Label: pcDNA6.2-GW/EmGFP-miR-SUMO23
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           86..112
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           176..217
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           224..250
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           314..355
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     protein_bind    complement(384..408)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(450..469)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    494..542
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(712..731)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(713..731)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(736..752)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(736..752)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tcggcatgga cgagctgtac aagtaagcta agcacttcgt ggccgtcgat cgtttaaagg
       61 gaggtagtga gtcgaccagt ggatcctgga ggcttgctga aggctgtatg ctgaatcgaa
      121 tctgcctcat tgacgttttg gccactgact gacgtcaatg acagattcga ttcaggacac
      181 aaggcctgtt actagcactc acatggaaca aatggcccag atcctggagg cttgctgaag
      241 gctgtatgct gtgatctgcc tcattgacaa acgttttggc cactgactga cgtttgtcag
      301 aggcagatca caggacacaa ggcctgttac tagcactcac atggaacaaa tggcccagat
      361 ctggccgcac tcgagatatc tagacccagc tttcttgtac aaagtggttg atctagaggg
      421 cccgcggttc gctgatgggg gaggctaact gaaacacgga aggagacaat accggaagga
      481 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt
      541 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgtgacccca
      601 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
      661 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcat cccctatagt
      721 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
      781 tgatggatct gcgcagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
      841 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgcccta
//
