LOCUS       sequence-35954-e         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Scott KL, Nogueira C, Heffernan TP, van Doorn R, Dhakal S, Hanna JA,
            Min C, Jaskelioff M, Xiao Y, Wu CJ, Cameron LA, Perry SR, Zeid R, 
            Feinberg T, Kim M, Vande Woude G, Granter SR, Bosenberg M, Chu GC, 
            DePinho RA, Rimm DL, Chin L
  TITLE     Proinvasion metastasis drivers in early-stage melanoma are 
            oncogenes.
  JOURNAL   Cancer Cell. 2011 Jul 12;20(1):92-103.
  PUBMED    21741599
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Cell."; date: "2011-07-12"; volume: "20(1)"; pages: "92-103"
COMMENT     Sequence Label: pLenti6/V5-DEST-ASF1B
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(24..65)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    118..142
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcaactcatt actaaccggt acgcgtagaa tcgagaccga ggagagggtt agggataggc
       61 ttaccttcga accgcgggcc ctctagactc gagcggccgc cactgtgctg gatatcaacc
      121 actttgtaca agaaagttgg gtagatgcag tccatggagt tctcagggag gaggccaggg
      181 atgcagccag ggagccccaa gcccttgata ggagtgcagt tgagtgggag gccgcagccc
      241 agggaggggt cctgggtctc tatggcctcc agcctgtcca tgttgttgtc ccagttgata
      301 tggaagcggg tcacccgggg gttcgaggcc aagatgttcc gctggagctg ggagaaatct
      361 ggcttcatgg gcgggttctc acgcagctca gggttgaggt actcgttgtt gacgtagtag
      421 cccactcgga tgaactcctg tccatggtag gtgcaggtga tgaggaccac agtcacaccc
      481 acggcatcag tctctgggat gagggatggg ttgggggcgt cggcctgaaa gacaaacatg
      541 tgtctccctg ctggcacagg gcccaccagc accgagtcta ggatctgatc aaattcctca
      601 ctctcagccg agccaacata aatgatcttc cactccaggt cgtccgccag ggcttcactg
      661 cactcgaagc tgatctcgaa ccggaagggg ctgtggaaag ggctcgggtt c
//
