LOCUS       sequence-359551-        1976 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector containing pTac promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Goosens VJ, Walker KT, Aragon SM, Singh A, Senthivel VR, Dekker L, 
            Caro-Astorga J, Buat MLA, Song W, Lee KY, Ellis T
  TITLE     Komagataeibacter Tool Kit (KTK): A Modular Cloning System for 
            Multigene Constructs and Programmed Protein Secretion from Cellulose
            Producing Bacteria.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3422-3434. doi: 
            10.1021/acssynbio.1c00358. Epub 2021 Nov 12.
  PUBMED    34767345
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00358"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3422-3434"
COMMENT     Sequence Label: KTK_247
FEATURES             Location/Qualifiers
     source          1..1976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(202..306)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      409..997
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     898..917
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1131..1136,1137..1152,1153..1159)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1165..1184
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     CDS             complement(join(1317..1976,1..132,133..201))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1940..1959
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
       61 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      121 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      181 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      241 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      301 tccgcgcaca tttccccgaa aagtgccacc tgtcatgacc aaaatccctt aacgtgagtt
      361 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
      421 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
      481 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
      541 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
      601 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
      661 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
      721 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
      781 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
      841 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
      901 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
      961 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     1021 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     1081 ttctgtggat aaccgtagtc ggtctctgac cagaacgatc gttggctgtg ttgacaatta
     1141 atcatcggct cgtataatgt gtggaattgt gagcgctcac aatttactag agagctacta
     1201 gtagcggcct ggctgagacc aagattatca aaaaggatct tcacctagat ccttttaaat
     1261 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     1321 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     1381 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     1441 gctgcaatga taccgcggga cccacgctca ccggctccag atttatcagc aataaaccag
     1501 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     1561 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     1621 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     1681 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     1741 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     1801 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     1861 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     1921 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgct
//
