LOCUS       sequence-359725-        9243 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CREB - Cyclic AMP gene reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9243)
  AUTHORS   O'Connell DJ, Kolde R, Sooknah M, Graham DB, Sundberg TB, Latorre 
            IJ, Mikkelsen TS, Xavier RJ
  TITLE     Simultaneous Pathway Activity Inference and Gene Expression Analysis
            Using RNA Sequencing.
  JOURNAL   Cell Syst. 2016 May 25;2(5):323-34. doi: 10.1016/j.cels.2016.04.011.
            Epub 2016 May 19.
  PUBMED    27211859
REFERENCE   2  (bases 1 to 9243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.04.011"; journalName: "Cell Syst"; date: 
            "2016-05-25- 25"; volume: "2"; issue: "5"; pages: "323-34"
COMMENT     Sequence Label: pLminP_Luc2P_RE16
FEATURES             Location/Qualifiers
     source          1..9243
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2075..2106
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      2133..2142
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2139..3788
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             3792..3911
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     misc_feature    4003..4591
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4056..4076)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4474..4485)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4606..4922
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4773..4908
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(4973..4975,4976..4978,4979..5692)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5018..5039)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5279..5298)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5626..5647
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5764..5997
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6069..6190
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6106..6125)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6160..6179
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6230..6365
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6385..6404)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6386..6404)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6414..6431)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6414..6430)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6423..6445)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6572..7027
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6659..6678)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6869..6890
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7053..7157
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7158..7226,7227..8018)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7376..7395)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8189..8777
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8678..8697
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8931..8948
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9065..9086
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9101..9106,9107..9124,9125..9131)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9139..9155
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9144..9166
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9163..9179
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9163..9179
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9198..9218
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9200..9218
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatcgc accagcgtgt ggatcacacc agacattgac gtaagctgcc agatcccatt
     1981 cccgtcatac tctgacgtct ttcagacacc ccattgacgt caatgggaga acgatccgag
     2041 aacagatctg gcctcggcgg ccaagcttag acactagagg gtatataatg gaagctcgac
     2101 ttccagcttg gcaatccggt actgttggta aagccaccat ggaagatgcc aaaaacatta
     2161 agaagggccc agcgccattc tacccactcg aagacgggac cgccggcgag cagctgcaca
     2221 aagccatgaa gcgctacgcc ctggtgcccg gcaccatcgc ctttaccgac gcacatatcg
     2281 aggtggacat tacctacgcc gagtacttcg agatgagcgt tcggctggca gaagctatga
     2341 agcgctatgg gctgaataca aaccatcgga tcgtggtgtg cagcgagaat agcttgcagt
     2401 tcttcatgcc cgtgttgggt gccctgttca tcggtgtggc tgtggcccca gctaacgaca
     2461 tctacaacga gcgcgagctg ctgaacagca tgggcatcag ccagcccacc gtcgtattcg
     2521 tgagcaagaa agggctgcaa aagatcctca acgtgcaaaa gaagctaccg atcatacaaa
     2581 agatcatcat catggatagc aagaccgact accagggctt ccaaagcatg tacaccttcg
     2641 tgacttccca tttgccaccc ggcttcaacg agtacgactt cgtgcccgag agcttcgacc
     2701 gggacaaaac catcgccctg atcatgaaca gtagtggcag taccggattg cccaagggcg
     2761 tagccctacc gcaccgcacc gcttgtgtcc gattcagtca tgcccgcgac cccatcttcg
     2821 gcaaccagat catccccgac accgctatcc tcagcgtggt gccatttcac cacggcttcg
     2881 gcatgttcac cacgctgggc tacttgatct gcggctttcg ggtcgtgctc atgtaccgct
     2941 tcgaggagga gctattcttg cgcagcttgc aagactataa gattcaatct gccctgctgg
     3001 tgcccacact atttagcttc ttcgctaaga gcactctcat cgacaagtac gacctaagca
     3061 acttgcacga gatcgccagc ggcggggcgc cgctcagcaa ggaggtaggt gaggccgtgg
     3121 ccaaacgctt ccacctacca ggcatccgcc agggctacgg cctgacagaa acaaccagcg
     3181 ccattctgat cacccccgaa ggggacgaca agcctggcgc agtaggcaag gtggtgccct
     3241 tcttcgaggc taaggtggtg gacttggaca ccggtaagac actgggtgtg aaccagcgcg
     3301 gcgagctgtg cgtccgtggc cccatgatca tgagcggcta cgttaacaac cccgaggcta
     3361 caaacgctct catcgacaag gacggctggc tgcacagcgg cgacatcgcc tactgggacg
     3421 aggacgagca cttcttcatc gtggaccggc tgaagagcct gatcaaatac aagggctacc
     3481 aggtagcccc agccgaactg gagagcatcc tgctgcaaca ccccaacatc ttcgacgccg
     3541 gggtcgccgg cctgcccgac gacgatgccg gcgagctgcc cgccgcagtc gtcgtgctgg
     3601 aacacggtaa aaccatgacc gagaaggaga tcgtggacta tgtggccagc caggttacaa
     3661 ccgccaagaa gctgcgcggt ggtgttgtgt tcgtggacga ggtgcctaaa ggactgaccg
     3721 gcaagttgga cgcccgcaag atccgcgaga ttctcattaa ggccaagaag ggcggcaaga
     3781 tcgccgtgaa ttctcacggc ttccctcccg aggtggagga gcaggccgcc ggcaccctgc
     3841 ccatgagctg cgcccaggag agcggcatgg atagacaccc tgctgcttgc gccagcgcca
     3901 ggatcaacgt ctaaggccgc gactctagaa cgcgtcactg cggcttacgg ggtgtataag
     3961 gcgactacag acatcagcgc gtaccggtta gtaatgatcg acaatcaacc tctggattac
     4021 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     4081 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     4141 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     4201 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     4261 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     4321 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     4381 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
     4441 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     4501 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     4561 agtcggatct ccctttgggc cgcctccccg cctggcgatg gtaccggtgt ggaaagtccc
     4621 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     4681 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     4741 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     4801 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     4861 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     4921 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcaccta gcatggtgag
     4981 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     5041 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     5101 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     5161 caccttcacc tacggcgtgc agtgcttcgc ccgctacccc gaccacatga agcagcacga
     5221 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     5281 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     5341 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     5401 gtacaactac aacagccaca aggtctatat caccgccgac aagcagaaga acggcatcaa
     5461 ggtgaacttc aagacccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     5521 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     5581 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     5641 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aaggtacctt
     5701 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     5761 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     5821 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     5881 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     5941 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     6001 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     6061 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     6121 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     6181 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     6241 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     6301 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     6361 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     6421 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     6481 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     6541 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     6601 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     6661 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     6721 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     6781 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     6841 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     6901 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     6961 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     7021 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     7081 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     7141 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     7201 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     7261 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     7321 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     7381 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     7441 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     7501 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     7561 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     7621 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     7681 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     7741 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     7801 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     7861 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     7921 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     7981 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     8041 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     8101 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     8161 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     8221 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     8281 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     8341 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     8401 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     8461 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     8521 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     8581 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     8641 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     8701 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     8761 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     8821 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     8881 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     8941 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     9001 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     9061 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     9121 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     9181 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
     9241 ctt
//
