LOCUS       sequence-360073-         460 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express PC(75-228)-H6 in E. coli. PC(75-228)-H6 was used for GST-CBP
            pull-down assays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Tie F, Banerjee R, Fu C, Stratton CA, Fang M, Harte PJ
  TITLE     Polycomb inhibits histone acetylation by CBP by binding directly to 
            its catalytic domain.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Feb 9;113(6):E744-53. doi: 
            10.1073/pnas.1515465113. Epub 2016 Jan 22.
  PUBMED    26802126
REFERENCE   2  (bases 1 to 460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1515465113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-02-9- 9"; volume: "113"; issue: "6"; pages: "E744-53"
COMMENT     Sequence Label: pET11-PC(75-228)-H6
FEATURES             Location/Qualifiers
     source          1..460
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             176..205
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             254..277
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gaacaaatcc tccggaactc cctccaagcg aggcattaag aagaaggaga aagaacccga
       61 tccggagccg gaatccgagg aggatgagta caccttcacc gaaaacgatg tggacacgca
      121 tcaagccacc acctcatcgg ctacccacga taaggagtcg aagaaggaga agaagcacca
      181 tcaccaccac caccatcatc accacatcaa gtccgaacgc aacagtggac ggcgctcgga
      241 atctccgctg acccaccatc atcatcacca ccaccacgag tccaagcgtc agcgcattga
      301 tcacagctcc tcctcgaaca gcagcttcac gcacaactca tttgtccccg agccggacag
      361 caactcctcc agctccgagg atcagccgct gatcggcacc aaacgcaaag ccgaggtgct
      421 caaggaatcg ggcaagattg gagttacgat taagacctcc
//
