LOCUS       sequence-360360-       10173 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This control vector contains a scrambled version of the targeting 
            sequence used in the pFUGW-shRII-alpha construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10173)
  AUTHORS   Church TW, Tewatia P, Hannan S, Antunes J, Eriksson O, Smart TG, 
            Hellgren Kotaleski J, Gold MG
  TITLE     AKAP79 enables calcineurin to directly suppress protein kinase A 
            activity.
  JOURNAL   Elife. 2021 Oct 6;10. pii: 68164. doi: 10.7554/eLife.68164.
  PUBMED    34612814
REFERENCE   2  (bases 1 to 10173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-10-6"; volume: "10. pii: 68164. doi"; pages: " 
            10.7554/eLife.68164"
COMMENT     Sequence Label: pFUGW-scrambled
FEATURES             Location/Qualifiers
     source          1..10173
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(32..49)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(203..791)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(283..302)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(962..1753,1754..1822))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1585..1604
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1823..1927)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2002..2021)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2193..2572
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2574..2772
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2727..2747
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2790..2970
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3017..3142
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3635..3868
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4053..4097
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4395..4512
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(4660..4874)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(4720..4739)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        4903..5302
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5951..5970
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      6077..6086
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6083..6085,6086..6088,6089..6802)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6083..6085,6086..6088,6089..6799)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6128..6149)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6389..6408)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6736..6757
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6829..7417
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6882..6902)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7300..7311)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7420..7436)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7421..7437)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7942..8122
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8139..8156)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8145..8369
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8415..8843
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8502..8521)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8712..8733
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8852..8872)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8857..9186
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9037..9172
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9099..9118
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9234..9281
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9300..9674
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9804..9925
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9841..9860)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9895..9914
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9974..9990)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9974..9990)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9987..10009)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9998..10014
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10022..10028,10029..10046,10047..10052))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10067..10088
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
       61 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
      121 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      181 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
      241 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      301 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      361 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
      421 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
      481 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
      541 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
      601 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
      661 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
      721 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
      781 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
      841 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
      901 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
      961 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     1021 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     1081 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     1141 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     1201 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     1261 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     1321 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     1381 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     1441 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     1501 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     1561 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     1621 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     1681 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     1741 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     1801 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     1861 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     1921 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga
     1981 tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct
     2041 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
     2101 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct
     2161 gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata
     2221 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     2281 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     2341 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     2401 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     2461 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     2521 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     2581 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     2641 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     2701 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     2761 ctatataagc agcgcgtttt gcctgtactg ggtctctctg gttagaccag atctgagcct
     2821 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     2881 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     2941 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     3001 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     3061 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     3121 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     3181 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     3241 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     3301 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     3361 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     3421 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     3481 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     3541 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     3601 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     3661 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     3721 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     3781 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     3841 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     3901 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     3961 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     4021 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     4081 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     4141 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     4201 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     4261 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     4321 gatccattcg attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca
     4381 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     4441 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     4501 attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggtt aattaagaaa
     4561 ggatccgaaa gaattcgccc ttagatctct agacaaaaaa gccacgtcat agagacactg
     4621 ttctcttgaa acagtgtctc tatgacgtgg cgggaaagag tggtctcata cagaacttat
     4681 aagattccca aatccaaaga catttcacgt ttatggtgat ttcccagaac acatagcgac
     4741 atgcaaatat tgcagggcgc cactcccctg tccctcacag ccatcttcct gccagggcgc
     4801 acgcgcgctg ggtgttcccg cctagtgaca ctgggcccgc gattccttgg agcgggttga
     4861 tgacgtcagc gttcttaatt aacccgtgtc ggctccagat ctggcctccg cgccgggttt
     4921 tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca gacgaagggc
     4981 gcagcgagcg tcctgatcct tccgcccgga cgctcaggac agcggcccgc tgctcataag
     5041 actcggcctt agaaccccag tatcagcaga aggacatttt aggacgggac ttgggtgact
     5101 ctagggcact ggttttcttt ccagagagcg gaacaggcga ggaaaagtag tcccttctcg
     5161 gcgattctgc ggagggatct ccgtggggcg gtgaacgccg atgattatat aaggacgcgc
     5221 cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg
     5281 atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg ctagccgggg ctttcgtggc
     5341 cgccgggccg ctcggtggga cggaagcgtg tggagagacc gccaagggct gtagtctggg
     5401 tccgcgagca aggttgccct gaactggggg ttggggggag cgcagcaaaa tggcggctgt
     5461 tcccgagtct tgaatggaag acgcttgtga ggcgggctgt gaggtcgttg aaacaaggtg
     5521 gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg cgggaaagct
     5581 cttattcggg tgagatgggc tggggcacca tctggggacc ctgacgtgaa gtttgtcact
     5641 gactggagaa ctcggtttgt cgtctgttgc gggggcggca gttatggcgg tgccgttggg
     5701 cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg tcgtgacgtc acccgttctg
     5761 ttggcttata atgcagggtg gggccacctg ccggtaggtg tgcggtaggc ttttctccgt
     5821 cgcaggacgc agggttcggg cctagggtag gctctcctga atcgacaggc gccggacctc
     5881 tggtgagggg agggataagt gaggcgtcag tttctttggt cggttttatg tacctatctt
     5941 cttaagtagc tgaagctccg gttttgaact atgcgctcgg ggttggcgag tgtgttttgt
     6001 gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa ttttcagtgt
     6061 tagactagta ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
     6121 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     6181 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     6241 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     6301 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     6361 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     6421 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     6481 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     6541 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     6601 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     6661 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     6721 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     6781 catggacgag ctgtacaagt aaagcaattc gatatcaagc ttatcgataa tcaacctctg
     6841 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     6901 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     6961 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     7021 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     7081 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     7141 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     7201 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     7261 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     7321 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     7381 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgagac
     7441 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc
     7501 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta
     7561 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     7621 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac
     7681 acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca
     7741 ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc
     7801 aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg
     7861 gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca catggcccga
     7921 gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     7981 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     8041 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     8101 tcagtgtgga aaatctctag cagaaacccg ctgatcagcc tcgactgtgc cttctagttg
     8161 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     8221 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     8281 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     8341 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     8401 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     8461 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     8521 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     8581 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     8641 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     8701 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     8761 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     8821 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     8881 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     8941 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     9001 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     9061 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     9121 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     9181 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca
     9241 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca
     9301 tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg
     9361 agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg
     9421 tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca
     9481 acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg
     9541 tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc
     9601 cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg
     9661 aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt
     9721 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     9781 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     9841 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     9901 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     9961 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
    10021 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
    10081 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
    10141 tgcattaatg aatcggccaa cgcgcgggga gag
//
