LOCUS       sequence-360365-       11236 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Replacement of endogenous rat PKA-RII-alpha subunits with the 
            RII-alpha S97A variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11236)
  AUTHORS   Church TW, Tewatia P, Hannan S, Antunes J, Eriksson O, Smart TG, 
            Hellgren Kotaleski J, Gold MG
  TITLE     AKAP79 enables calcineurin to directly suppress protein kinase A 
            activity.
  JOURNAL   Elife. 2021 Oct 6;10. pii: 68164. doi: 10.7554/eLife.68164.
  PUBMED    34612814
REFERENCE   2  (bases 1 to 11236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-10-6"; volume: "10. pii: 68164. doi"; pages: " 
            10.7554/eLife.68164"
COMMENT     Sequence Label: pFUGW-shRII-alpha-RII-alpha-S97A-IRES-EGFP
FEATURES             Location/Qualifiers
     source          1..11236
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(319..893,894..896,897..905)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(486..503)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     713..732
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(906..908,909..911,912..1625)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(906..908,909..911,912..1622)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(951..972)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1212..1231)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1559..1580
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1652..2240
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1705..1725)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2123..2134)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2243..2259)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2244..2260)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2765..2945
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2962..2979)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2968..3192
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3238..3666
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3325..3344)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3535..3556
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3675..3695)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3680..4009
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3860..3995
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3922..3941
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4057..4104
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4123..4497
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4627..4748
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4664..4683)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4718..4737
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4797..4813)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4797..4813)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4810..4832)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4821..4837
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4845..4851,4852..4869,4870..4875))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4890..4911
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5028..5045)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5199..5787)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5279..5298)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5958..6749,6750..6818))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6581..6600
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6819..6923)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6998..7017)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7189..7568
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7570..7768
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7723..7743
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7786..7966
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8013..8138
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8631..8864
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9049..9093
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9391..9508
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(9656..9870)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(9716..9735)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        9899..10298
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
ORIGIN
        1 ctggagaagt gagcatcttg attagaagca agactaaaac gaacaagaac ggcgggaacc
       61 aggaggttga gattgcccac tgccataagg ggcagtactt tggagaactt gccctggtga
      121 ccaacaaacc aagagctgct tctgcttatg cggttggaga cgtcaaatgc ttagtcatgg
      181 atgttcaagc atttgagagg cttctgggcc cctgcatgga catcatgaag aggaacatct
      241 cacattacga agaacagctg gtgaagatgt ttggctccaa cttggatcta ttggaccccg
      301 ggcagtagct cggatccgcc cctctccctc ccccccccnt aacgttactg gccgaagccg
      361 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
      421 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
      481 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
      541 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
      601 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
      661 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
      721 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
      781 tgatctgggg cctcggtaca catgctttac atgtgtttag tcgaggttaa aaaaacgtct
      841 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca
      901 caaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
      961 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     1021 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     1081 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     1141 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     1201 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     1261 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     1321 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     1381 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     1441 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     1501 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     1561 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     1621 agtaaagcaa ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga
     1681 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     1741 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     1801 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     1861 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     1921 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     1981 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     2041 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     2101 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     2161 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     2221 cctttgggcc gcctccccgc atcgataccg tcgacctcga gacctagaaa aacatggagc
     2281 aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga
     2341 ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa
     2401 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     2461 ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
     2521 tgattggcag aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg
     2581 ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac
     2641 ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga
     2701 gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg
     2761 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     2821 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     2881 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     2941 tagcagaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     3001 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     3061 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     3121 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     3181 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     3241 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     3301 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     3361 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     3421 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     3481 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     3541 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     3601 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     3661 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     3721 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     3781 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     3841 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     3901 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     3961 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     4021 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
     4081 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt
     4141 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg
     4201 ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg
     4261 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
     4321 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc
     4381 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc
     4441 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac
     4501 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     4561 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     4621 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     4681 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     4741 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     4801 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     4861 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     4921 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     4981 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     5041 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     5101 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     5161 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     5221 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     5281 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     5341 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     5401 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     5461 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     5521 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     5581 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     5641 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     5701 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     5761 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     5821 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     5881 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     5941 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     6001 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     6061 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     6121 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     6181 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     6241 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     6301 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     6361 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     6421 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     6481 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     6541 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     6601 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     6661 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     6721 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     6781 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     6841 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     6901 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
     6961 tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat
     7021 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
     7081 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
     7141 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat
     7201 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     7261 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     7321 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     7381 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     7441 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     7501 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     7561 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
     7621 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     7681 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
     7741 gtgtacggtg ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta
     7801 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     7861 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     7921 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     7981 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     8041 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     8101 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     8161 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     8221 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     8281 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     8341 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     8401 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     8461 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     8521 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     8581 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     8641 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     8701 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     8761 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     8821 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     8881 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     8941 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     9001 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     9061 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     9121 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     9181 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     9241 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     9301 gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt
     9361 ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     9421 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     9481 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt
     9541 ttggttaatt aagaaaggat ccgaaagaat tcgcccttag atctctagac aaaaaagcca
     9601 ggaatcagac tcgttcatct cttgaatgaa cgagtctgat tcctggcggg aaagagtggt
     9661 ctcatacaga acttataaga ttcccaaatc caaagacatt tcacgtttat ggtgatttcc
     9721 cagaacacat agcgacatgc aaatattgca gggcgccact cccctgtccc tcacagccat
     9781 cttcctgcca gggcgcacgc gcgctgggtg ttcccgccta gtgacactgg gcccgcgatt
     9841 ccttggagcg ggttgatgac gtcagcgttc ttaattaacc cgtgtcggct ccagatctgg
     9901 cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc tcctcacggc gagcgctgcc
     9961 acgtcagacg aagggcgcag cgagcgtcct gatccttccg cccggacgct caggacagcg
    10021 gcccgctgct cataagactc ggccttagaa ccccagtatc agcagaagga cattttagga
    10081 cgggacttgg gtgactctag ggcactggtt ttctttccag agagcggaac aggcgaggaa
    10141 aagtagtccc ttctcggcga ttctgcggag ggatctccgt ggggcggtga acgccgatga
    10201 ttatataagg acgcgccggg tgtggcacag ctagttccgt cgcagccggg atttgggtcg
    10261 cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct gctgggctag
    10321 caagcttcga attccaccat gagccacatc cagatcccac cggggctcac ggagctgctg
    10381 cagggctaca ccgtggaggt gcttcggcag cagccgcccg acctcgtcga cttcgcggtg
    10441 gagtacttca cacgcctgcg cgaggcccgc cggcaagagt cagactcgtt catcgccccc
    10501 ccgacgacct ttcacgcgca ggagtccagc ggggtccccg tcatcgagga ggacggggag
    10561 agtgaatcgg actcggacga tgaggatctg gaagttccga ttcccagcaa atttactaga
    10621 cgagtagcag tctgtgcaga aacgtttaac cctgatgaag aagaagataa tgatccaagg
    10681 gtggttcacc ccaaaaccga cgagcagagg tgcagacttc aggaagcctg taaagacatt
    10741 ctgcttttca aaaacctgga tcaggaacag ctttctcaag ttctggacgc catgtttgaa
    10801 aagatagtca aaactgacga gcatgtcatt gaccaaggag atgatggaga caacttttat
    10861 gtcatagaaa ggggaaccta tgacatttta gtaacaaaag ataatcaaac acgatctgtt
    10921 ggtcagtatg acaaccgtgg cagttttgga gaactagccc tgatgtacaa taccccgaga
    10981 gctgctacca ttgtggccac ctcagacggc tccctttggg gattggaccg ggtgactttt
    11041 aggagaatca tagtgaagaa caatgcaaag aagaggaaga tgttcgaatc gtttattgag
    11101 tctgtaccgc tctttaaatc actagagatg tcagaacgaa tgaagattgt ggatgtgatc
    11161 ggggaaaaga tctataagga tggagagcga ataatcactc agggtgaaaa ggccgacagc
    11221 ttttatatta tagagt
//
