LOCUS       sequence-360369-        7426 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7426)
  AUTHORS   Hahn WC, Dessain SK, Brooks MW, King JE, Elenbaas B, Sabatini DM, 
            DeCaprio JA, Weinberg RA
  TITLE     Enumeration of the simian virus 40 early region elements necessary 
            for human cell transformation.
  JOURNAL   Mol Cell Biol 2002 Apr;22(7):2111-23.
  PUBMED    11884599
REFERENCE   2  (bases 1 to 7426)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol"; date: "2002-04"; volume: "22(7)"; pages: "2111-23"
COMMENT     Sequence Label: pBABE-neo largeTcDNA H42Q
FEATURES             Location/Qualifiers
     source          1..7426
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..470
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    535..734
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             935..1351
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1281..1303
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1319..1335
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1389..3515
                     /codon_start=1
                     /product="large T antigen from SV40 (simian virus 40)"
                     /label=large T antigen
                     /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFQPDK
                     GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLF
                     CSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYT
                     TKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLI
                     CKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETK
                     CDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQA
                     VDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLP
                     KMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLN
                     FELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKR
                     TQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALL
                     LMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDD
                     DEDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFT
                     CFKKPPTPPPEPET"
     primer_bind     complement(3558..3578)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3563..3892
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3743..3878
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4249..5043
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4303..4322)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4913..4932
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5566..5585
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             5847..6316
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(join(6431..7222,7223..7291))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7054..7073
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7292..7396)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
       61 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
      121 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
      181 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      241 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
      301 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
      361 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
      421 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
      481 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggcaagctg
      541 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
      601 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
      661 gttctgaaca cccggccgca accctgggag acgtcccagg gactttgggg gccgtttttg
      721 tggcccgacc tgaggaaggg agtcgatgtg gaatccgacc ccgtcaggat atgtggttct
      781 ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc tttcggtttg
      841 gaaccgaagc cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt gttgtctctg
      901 tctgactgtg tttctgtatt tgtctgaaaa ttagggccag actgttacca ctcccttaag
      961 tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt cggtagatgt
     1021 caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta acgtcggatg
     1081 gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca aggtcttttc
     1141 acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg aagccttggc
     1201 ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc ctcctcttcc
     1261 tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc gatcctccct
     1321 ttatccagcc ctcactcctt ctctaggcgc cggccggatc ccagtgtggt ggtacgtagg
     1381 aattcaccat ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag
     1441 gtcttgaaag gagtgcctgg gggaatattc ctctgatgag aaaggcatat ttaaaaaaat
     1501 gcaaggagtt tcagcctgat aaaggaggag atgaagaaaa aatgaagaaa atgaatactc
     1561 tgtacaagaa aatggaagat ggagtaaaat atgctcatca acctgacttt ggaggcttct
     1621 gggatgcaac tgagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct
     1681 ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg
     1741 ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact
     1801 ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt
     1861 gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa
     1921 aatattctgt aacctttata agtaggcata acagttataa tcataacata ctgttttttc
     1981 ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct
     2041 ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta
     2101 gagatccatt ttctgttatt gaggaaagtt tgccaggtgg gttaaaggag catgatttta
     2161 atccagaaga agcagaggaa actaaacaag tgtcctggaa gcttgtaaca gagtatgcaa
     2221 tggaaacaaa atgtgatgat gtgttgttat tgcttgggat gtacttggaa tttcagtaca
     2281 gttttgaaat gtgtttaaaa tgtattaaaa aagaacagcc cagccactat aagtaccatg
     2341 aaaagcatta tgcaaatgct gctatatttg ctgacagcaa aaaccaaaaa accatatgcc
     2401 aacaggctgt tgatactgtt ttagctaaaa agcgggttga tagcctacaa ttaactagag
     2461 aacaaatgtt aacaaacaga tttaatgatc ttttggatag gatggatata atgtttggtt
     2521 ctacaggctc tgctgacata gaagaatgga tggctggagt tgcttggcta cactgtttgt
     2581 tgcccaaaat ggattcagtg gtgtatgact ttttaaaatg catggtgtac aacattccta
     2641 aaaaaagata ctggctgttt aaaggaccaa ttgatagtgg taaaactaca ttagcagctg
     2701 ctttgcttga attatgtggg gggaaagctt taaatgttaa tttgcccttg gacaggctga
     2761 actttgagct aggagtagct attgaccagt ttttagtagt ttttgaggat gtaaagggca
     2821 ctggagggga gtccagagat ttgccttcag gtcagggaat taataacctg gacaatttaa
     2881 gggattattt ggatggcagt gttaaggtaa acttagaaaa gaaacaccta aataaaagaa
     2941 ctcaaatatt tccccctgga atagtcacca tgaatgagta cagtgtgcct aaaacactgc
     3001 aggccagatt tgtaaaacaa atagatttta ggcccaaaga ttatttaaag cattgcctgg
     3061 aacgcagtga gtttttgtta gaaaagagaa taattcaaag tggcattgct ttgcttctta
     3121 tgttaatttg gtacagacct gtggctgagt ttgctcaaag tattcagagc agaattgtgg
     3181 agtggaaaga gagattggac aaagagttta gtttgtcagt gtatcaaaaa atgaagttta
     3241 atgtggctat gggaattgga gttttagatt ggctaagaaa cagtgatgat gatgatgaag
     3301 acagccagga aaatgctgat aaaaatgaag atggtgggga gaagaacatg gaagactcag
     3361 ggcatgaaac aggcattgat tcacagtccc aaggctcatt tcaggcccct cagtcctcac
     3421 agtctgttca tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     3481 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     3541 ggggatctcg accctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     3601 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     3661 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     3721 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     3781 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     3841 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttca
     3901 cgctgccgca agcactcagg gcgcaagggc tgctaaagga agcggaacac gtagaaagcc
     3961 agtccgcaga aacggtgctg accccggatg aatgtcagct actgggctat ctggacaagg
     4021 gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg gcgatagcta
     4081 gactgggcgg ttttatggac agcaagcgaa ccggaattgc cagctggggc gccctctggt
     4141 aaggttggga agccctgcaa agtaaactgg atggctttct tgccgccaag gatctgatgg
     4201 cgcaggggat caagatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     4261 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     4321 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     4381 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
     4441 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     4501 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     4561 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     4621 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     4681 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     4741 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
     4801 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     4861 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     4921 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     4981 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     5041 tgagcgggac tctggggttc gcatcgatgc atcgatgcgg cgggtgtggt ggttacgcgt
     5101 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     5161 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     5221 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     5281 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     5341 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     5401 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     5461 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     5521 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     5581 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     5641 gtcagggggg cggagcctat ggaaaaacgc cagcaacgca tcgataaaat aaaagatttt
     5701 atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc
     5761 ttaagtaacg ccattttgca aggcatggaa aaatacataa ctgagaatag agaagttcag
     5821 atcaaggtca ggaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag
     5881 cagttcctgc cccggctcag ggccaagaac agatggaaca gctgaatatg ggccaaacag
     5941 gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat
     6001 gcggtccagc cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga
     6061 cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc
     6121 gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc ggggcgccag
     6181 tcctccgatt gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc
     6241 atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt
     6301 cagcgggggt ctttcacatg cagcatgtat caaaattaat ttggtttttt ttcttaagta
     6361 tttacattaa atggccatag tgcggccgca aatcaatcta aagtatatat gagtaaactt
     6421 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     6481 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     6541 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     6601 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     6661 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     6721 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     6781 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     6841 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     6901 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     6961 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     7021 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     7081 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     7141 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     7201 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     7261 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     7321 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     7381 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgcagc
//
