LOCUS       sequence-360375-       10678 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsTNRC6A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10678)
  AUTHORS   Lazzaretti D, Tournier I, Izaurralde E
  TITLE     The C-terminal domains of human TNRC6A, TNRC6B, and TNRC6C silence 
            bound transcripts independently of Argonaute proteins.
  JOURNAL   RNA. 2009 Jun . 15(6):1059-66.
  PUBMED    19383768
REFERENCE   2  (bases 1 to 10678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2009 
            Jun . 15(6):1059-66."
COMMENT     Sequence Label: pCIneo-lambdaN-HA-HsTNRC6A_C
FEATURES             Location/Qualifiers
     source          1..10678
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1089..1098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1095..1160
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             1179..1205
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    5420..5431
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     polyA_signal    6382..6503
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6393..6412)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6447..6466
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      6689..7144
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6776..6795)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6986..7007
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7261..7618
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7261..7452
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7284..7304)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7469..7604
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7669..8463
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7723..7742)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8333..8352
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8527..8575
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(8616..8635)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8735..8757
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8795..8813)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8881..8985
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8986..9054,9055..9846)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9204..9223)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10017..10605
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10506..10525
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag
     1141 ctcaatggaa agctgcaaac ccaccgctcg agatggctta cccatacgat gttccagatt
     1201 acgctatgga tgctgattct gcctccagtt ctgaatcaga gagaaacatc actatcatgg
     1261 cttcagggaa cacaggtggt gaaaaagatg gccttcggaa tagcactgga cttggttccc
     1321 aaaacaagtt tgtagttggt agcagcagca ataatgtggg ccatggaagt agtactgggc
     1381 catggggttt ttcccatgga gccataataa gcacatgtca ggtctctgtg gatgctcctg
     1441 aaagcaaatc tgaaagtagc aacaatagaa tgaatgcttg gggcactgta agttcttcat
     1501 caaatggagg gttaaatcca agcactttga attcagctag caaccatggt gcctggccag
     1561 tattagagaa caatggactt gccctaaaag ggcctgtagg gagtggtagt tctggcatta
     1621 atattcagtg cagtactata ggccagatgc ctaacaatca gagtattaac tctaaagtga
     1681 gtggtggttc tacccatggt acctggggaa gccttcagga aacttgtgaa tctgaagtaa
     1741 gtggtacaca gaaggtttca ttcagtggtc aacctcaaaa tattaccact gaaatgactg
     1801 gaccaaataa cactactaac tttatgacct ctagtttacc aaactccggt tcagtgcaga
     1861 ataatgagct gcctagtagt aacacagggg cctggcgtgt gagcacaatg aatcatcctc
     1921 agatgcaggc tccatcaggt atgaatggca cttccctttc tcaccttagc aatggagagt
     1981 caaaaagtgg aggctcttat ggtactacat ggggtgccta tggttctaat tactctggag
     2041 acaaatgttc aggccctaat ggccaagcta atggtgacac tgtgaatgca actctaatgc
     2101 agcctggcgt aaatggtcct atgggcacta actttcaagt taacacaaac aaaggaggtg
     2161 gtgtgtggga atctggtgca gcaaactccc agagtacatc atggggaagt ggaaatggca
     2221 caaattctgg aggaagtcga agaggatggg gaacccctgc acaaaacact ggcactaatt
     2281 tacccagcgt tgagtggaac aaactgccta gcaatcagca ttccaatgat agtgcaaatg
     2341 gcaatggtaa gacgtttaca aatggatgga aatctactga ggaagaggat cagggttctg
     2401 ccacatctca gacaaatgag caaagcagtg tgtgggccaa aacaggaggt acagtggaga
     2461 gcgatggtag tacagaaagc actggacgcc ttgaggaaaa aggaactggg gaaagtcaga
     2521 gtagagacag aagaaaaatt gatcagcaca cattactcca aagcattgta aacagaactg
     2581 acttagatcc acgtgtcctg tccaactctg gttggggaca gactcctatt aagcagaata
     2641 ctgcctggga tacagaaaca tcacctagag gggaacgaaa gactgacaat gggacagagg
     2701 cctggggaag ctctgcaaca cagactttta actcaggggc atgtatagat aagactagcc
     2761 ctaatggtaa tgatacctca tctgtatcag ggtggggcga tcccaaacct gctctgaggt
     2821 ggggagattc caaaggctca aactgccagg gggggtggga agatgattct gctgctacag
     2881 gaatggtcaa gagcaatcag tgggggaatt gcaaagagga gaaggctgca tggaatgact
     2941 cgcaaaagaa taaacaggga tggggtgatg gacaaaaatc aagccaaggg tggtctgttt
     3001 ctgccagtga taactgggga gaaacttcaa ggaataacca ttggggtgag gccaataaga
     3061 aatccagctc aggaggtagt gacagtgaca ggtccgtttc cggttggaac gaacttggta
     3121 aaactagttc tttcacttgg ggaaacaaca taaatccaaa taattcatca ggatgggatg
     3181 aatcttctaa acctactcct tcccagggat ggggagaccc tccaaagtct aatcagtctc
     3241 taggttgggg agattcgtca aagccagtca gctctccaga ctggaacaag caacaagaca
     3301 ttgttggatc ttggggaatc ccaccagcta caggcaaacc tcctggtaca ggctggctgg
     3361 ggggacctat accagcccca gcaaaagaag aagaacccac aggctgggag gaaccatccc
     3421 cagaatctat acgtcgcaaa atggagattg atgatggaac ttcagcttgg ggagatccaa
     3481 gcaaatacaa ctacaaaaat gtgaacatgt ggaacaaaaa cgtcccaaat ggcaacagcc
     3541 gttcagacca gcaagcacag gtacatcagc tgctaacgcc tgcaagtgcc atctcaaaca
     3601 aagaggcaag cagtggctct ggctggggtg agccctgggg ggagccttct actccagcca
     3661 caactgtgga taatggtact tcagcatggg gtaagcccat agacagtggt cccagctggg
     3721 gggaacccat tgctgcggca tccagcacat ccacgtgggg ctccagctct gttggtccac
     3781 aagcattaag caaatctggg ccaaaatcta tgcaagatgg ctggtgtggt gatgatatgc
     3841 cattgcctgg aaatcgcccc actggctggg aagaggaaga ggatgtggag attggaatgt
     3901 ggaatagtaa ttcatctcaa gagcttaact catctttaaa ttggccacca tatacaaaga
     3961 aaatgtcatc gaagggtctg agtggcaaaa aaaggagaag ggaaggggga atgatgaaag
     4021 gtggaaacaa acaagaagaa gcgtggataa atccatttgt taaacagttt tcaaacatca
     4081 gtttttcgag agactcacca gaggaaaatg tacaaagcaa taagatggac ctttctggag
     4141 gaatgttaca agacaaacga atggagatag ataaacatag cctaaatatt ggtgattaca
     4201 atcgaacggt cgggaaaggc cctggttctc ggcctcagat ttccaaagag tcttccatgg
     4261 agcgcaatcc ttattttgat aaggatggca ttgtagcaga tgaatcccaa aacatgcagt
     4321 ttatgtccag tcaaagcatg aagcttcccc cttcaaatag tgcactacct aaccaggccc
     4381 ttggctccat agcagggctg ggtatgcaaa acttgaattc tgttagacag aatggcaatc
     4441 ccagtatgtt tggtgttgga aacacagcag cacaaccccg gggcatgcag cagcctccag
     4501 cacaacctct tagttcatct cagcctaatc tccgtgctca agtgcctcct ccattactct
     4561 cccctcaggt tccagtttca ttgctgaagt atgcaccaaa caacggtggc ctgaatccac
     4621 tctttggccc tcaacaggta gccatgctga accagctatc ccagctaaac cagctttctc
     4681 agatctccca gttacagcga ttgttagcgc agcagcaaag ggcgcagagt cagagaagcg
     4741 tgccttctgg gaaccggccg cagcaagacc agcagggtcg acctcttagt gtgcagcagc
     4801 aaatgatgca acaatctcgt caacttgatc caaacctgtt ggtgaagcag cagactccac
     4861 catctcagca gcagccactc catcagccag ccatgaagtc tttccttgac aatgtcatgc
     4921 cccacactac acctgagctg caaaaagggc catcaccaat aaatgctttc agcaacttcc
     4981 ctataggctt gaactcaaac ttgaatgtaa atatggatat gaacagtatt aaagagccac
     5041 agtcaagact aaggaagtgg acgacagtgg gcagcatttc tgtgaacaca tctttggatc
     5101 aaaactccag caaacatggt gctatttcaa gtggtttcag gctggaagag tctccatttg
     5161 ttccctatga ctttatgaac agcagtactt caccagccag tcctccaggt tcaataggag
     5221 atggctggcc acgtgccaaa tcgcctaacg gctctagcag tgttaattgg ccaccagaat
     5281 ttcgtcctgg tgagccatgg aaaggttatc caaacattga ccctgaaact gacccttacg
     5341 tcactcctgg cagtgtcata aacaatcttt caattaatac tgtgcgggaa gttgaccacc
     5401 tcagggacag gaacagtggg tcatcctcat ccttgaacac cacgctgcct tcaactagtg
     5461 cctggtcatc cattcgtgcc tccaactaca acgttcccct cagcagtaca gcacaaagca
     5521 cttcagccag aaatagtgat tccaaattga catggtctcc tggttcagtt acaaacacct
     5581 ctctggctca tgagctgtgg aaggtccctt tgccacctaa aaacatcact gctccgtccc
     5641 gcccacctcc gggactgact ggtcagaagc cacccttgtc tacgtgggat aattctcccc
     5701 ttcgtatagg tggaggatgg ggaaattctg acgccagata taccccaggt tccagctggg
     5761 gtgagagcag ctcagggaga ataacaaatt ggcttgttct aaaaaacctt acacctcaga
     5821 tcgatggctc aactctgcgc actctgtgca tgcagcacgg cccgctgatc acattccacc
     5881 tgaacctccc tcacggaaat gctctggtcc gctacagttc aaaagaagag gtagtggagg
     5941 cacaaaagtc tctgcacatg tgtgtactgg ggaacactac tattcttgct gagtttgcca
     6001 gtgaagagga gatcagtcgt ttctttgcac aaagccagtc tctgacccct tctcccggct
     6061 ggcagtctct cgggtccagc cagagccggc tgggctccct cgactgttcc cactcattct
     6121 ccagccggac cgatctcaat cactggaatg gtgctgggct gtcgggaact aactgtggag
     6181 accttcacgg cacttcactc tgggggaccc cgcattattc cacaagcctg tggggtcccc
     6241 caagcagcag cgacccccga ggaattagca gcccatctcc cattaacgct tttctttctg
     6301 ttgaccacct gggtgggggt ggagagtcca tgtaagcggc cgcttccctt tagtgagggt
     6361 taatgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     6421 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     6481 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     6541 agggggagat gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcc
     6601 gataaggatc gatccgggct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     6661 acagttgcgc agcctgaatg gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg
     6721 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     6781 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     6841 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     6901 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     6961 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     7021 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     7081 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     7141 atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgcg
     7201 gatctgcgca gcaccatggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt
     7261 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     7321 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     7381 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     7441 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     7501 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     7561 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     7621 gcttgattct tctgacacaa cagtctcgaa cttaaggcta gagccaccat gattgaacaa
     7681 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     7741 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     7801 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
     7861 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     7921 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     7981 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     8041 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     8101 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     8161 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
     8221 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     8281 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     8341 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     8401 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     8461 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgat
     8521 ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgaatcg
     8581 atagcgataa ggatccgcgt atggtgcact ctcagtacaa tctgctctga tgccgcatag
     8641 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     8701 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     8761 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     8821 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     8881 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     8941 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     9001 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     9061 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     9121 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     9181 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     9241 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     9301 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     9361 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     9421 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     9481 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     9541 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     9601 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     9661 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     9721 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     9781 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     9841 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     9901 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     9961 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
    10021 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
    10081 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
    10141 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
    10201 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
    10261 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
    10321 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
    10381 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
    10441 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
    10501 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
    10561 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
    10621 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatggctcg acagatct
//
