LOCUS       sequence-360574-        9366 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing HIV Envelope UFO.750.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9366)
  TITLE     Lentiviral vector expressing HIV envelope UFO.664
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGAE-UFO.750
FEATURES             Location/Qualifiers
     source          1..9366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        170..473
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        474..677
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      817..826
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3078..3666
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3131..3151)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3549..3560)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        complement(4413..4431)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4452..4468)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4452..4468)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4465..4487)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4476..4492
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4500..4506,4507..4524,4525..4530))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4545..4566
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4683..4700)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4854..5442)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4934..4953)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5613..6404,6405..6473))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6236..6255
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6474..6578)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6604..7059)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6741..6762)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6953..6972
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7186..7208
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7200..7217
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7201..7217
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7224..7243
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7224..7242
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        7357..7736
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7737..7940
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 aaatgctagc taagttaagg caggggtata ggccagtgtt ctcttcccca ccctcttatt
       61 tccagtagac tcatacccaa caggacccgg cactgccaac cagagaaggc aaagaaggag
      121 acggtggaga aggcggtgga tcctattatc gataagcttg ggagttccgc gttacataac
      181 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      241 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      301 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
      361 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
      421 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      481 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      541 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      601 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
      661 tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct
      721 gttttgacct ccatagaaga caccgggacc gatccagcct ccgcggccgg gaacggtgca
      781 ttggaacgcg gattccccgt gccaagagtg agatccgcca ccatggacag agccaaactc
      841 ctccttctcc ttttgctcct gctgctgcct caggcccagg ccgtggaaaa cctgtgggtc
      901 accgtgtact acggcgtgcc cgtgtggaag gacgccgaga caaccctgtt ctgtgccagc
      961 gacgccaagg cctacgacac cgaagtgcgg aatgtgtggg ccacccacgc ctgcgtgccc
     1021 accgatccta acccccagga aatcgtgctg gaaaacgtga ccgagaactt caacatgtgg
     1081 aagaacaaca tggtggaaca gatgcacacc gacatcatca gcctgtggga ccagagcctg
     1141 aagccctgcg tgaagctgac ccctctgtgc gtgaccctga actgcaccaa cgtgaacgtg
     1201 accaacacca ccaacaacac cgaggaaaag ggcgagatca agaactgcag cttcaacatc
     1261 accaccgagc tgcgggacaa gaaaaagaag gtgtacgccc tgttctaccg gctggacgtg
     1321 gtgcccatcg acgataacaa caacaactcc agcaattacc ggctgatcaa ctgcaacacc
     1381 agcgccatca cccaggcctg ccccaaggtg tccttcgagc ccatccccat ccactactgt
     1441 gcccctgccg gcttcgccat cctgaagtgc aacgacaaga agttcaacgg caccggcccc
     1501 tgcaagaacg tgtccaccgt gcagtgtacc cacggcatca agcccgtggt gtccacccag
     1561 ctgctgctga atggcagcct ggccgaggaa gagatcatca tcagaagcga gaacatcaca
     1621 aacaacgcca agaccatcat cgtgcagctg aacgagagcg tggaaatcaa ttgcacccgg
     1681 cccaacaaca ataccagaaa gagcatccgg atcggccctg gccagtggtt ttacgccacc
     1741 ggcgatatca tcggcgacat cagacaggcc cactgcaaca tctccggcac caagtggaac
     1801 aagaccctgc aacaggtcgt gaagaagctg agagagcact tcaacaacaa gacaatcatc
     1861 ttcaacccca gctctggcgg cgacctggaa atcaccaccc acagcttcaa ttgtggcggc
     1921 gagttcttct actgcaatac ctccggcctg ttcaacagca cctggatcgg caatggcacc
     1981 aagaacaaca acaacaccaa cgacaccatc accctgccct gcagaatcaa gcagatcatc
     2041 aatatgtggc agcgcgtggg ccagcctatg tacgcccctc ctattcaggg caagatcaga
     2101 tgcgtgtcca atatcaccgg cctgctgctg accagggacg gcggaaacaa caacacaaat
     2161 gagacagaga cattcagacc cggcggaggc gacatgagag acaactggcg gagcgagctg
     2221 tacaagtaca aggtcgtgaa aatcgagccc ctgggcgtgg cccccacccg gtgcaagaga
     2281 agagtggtgg aaggcggcgg aggatctggc ggagggggat ctgctgtggg aatcggcgct
     2341 gtgttcctgg gctttctggg agccgccgga agcacaatgg gcgctgccag catgaccctg
     2401 accgtgcagg ccagaaatct gctgtctggc ggcagcggct ctggctctgg atctacagtg
     2461 tgggggatca agcagctgca ggctagagtg ctggctgtgg aaagatacct gcgggaccag
     2521 cagctgctgg gcatctgggg ctgtagcggc aagctgatct gctgcaccaa tgtgccctgg
     2581 aacagctcct ggtccaacaa gagccaggac gagatctggg acaacatgac ctggatggaa
     2641 tgggacaaag agattaacaa ctacacagat atcatctaca gcctgatcga ggaaagccag
     2701 aaccagcagg aaaagaacga gcaggacctg ctggccctgg acaagtgggc ctccctgtgg
     2761 aattggttcg acatcaccaa ttggctgtgg tacatcaaga tcttcatcat gatcgtgggc
     2821 ggcctgatcg gcctgcggat cgtgtttgcc gtgctgagca tcgtgaacag agtgcggcag
     2881 ggcatcagcc ccctgagctt ccagaccctg atccccaacc ccagaggccc cgatagaccc
     2941 gagggcattg aagaagaggg cggcgagcag gatcgggaca gatccatccg gctcgtgaac
     3001 tgatgagcgg ccgctcgacg gtaccgagct cggatccact agtaacggcc gccagtgtgc
     3061 tggaattcgg ctttgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     3121 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     3181 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     3241 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     3301 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     3361 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     3421 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     3481 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     3541 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     3601 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     3661 ccccgcatcg gagccgaatt ctgcagatat ccatcacact ggcggccgct cgagttttat
     3721 aaaagaaaag gggggactgg aagggattta ttacagtgca agaagacata gaatcttaga
     3781 catgtactta gaaaaggaag aaggcatcat accagattgg caggattaca cctcaggacc
     3841 aggaattaga tacccaaaga catttggctg gctatggaaa ttagtccctg taaatgtatc
     3901 agatgaggca caggaggatg agaggcatta tttaatgcag ccagctcaaa cttccaagtg
     3961 ggatgaccct tggggagagg ttctagcgtg gaagtttgat ccaactctag cctacactta
     4021 tgaggcatat gctagatacc cagaagagtt ggaagcaagt caggcctgtc agaactgcat
     4081 ttcgctctgt attcagtcgc tctgcggaga ggctggcaga ttgagccctg ggaggttctc
     4141 tccagcacta gcaggtagag cctgggtgtt ccctgctaga ctctcaccag cacttggcca
     4201 gtgctgggca gagtggctcc acgcttgctt gcttaaagac ctcttcaata aagctgccat
     4261 tttagaagta agccagtgtg tgttcccatc tctcctagtc gccgcctggt caactcggta
     4321 ctcggtaata agaagaccct ggtctgttag gaccctttct gctttgagaa accgaagcag
     4381 gaaaatccct agcatggtac cagcttttgt tccctttagt gagggttaat ttcgagcttg
     4441 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     4501 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     4561 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     4621 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     4681 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     4741 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     4801 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     4861 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     4921 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     4981 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     5041 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     5101 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     5161 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     5221 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     5281 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     5341 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     5401 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     5461 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     5521 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     5581 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     5641 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     5701 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     5761 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     5821 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     5881 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     5941 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     6001 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     6061 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     6121 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     6181 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     6241 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     6301 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     6361 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     6421 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     6481 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     6541 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     6601 cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     6661 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     6721 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     6781 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     6841 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     6901 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     6961 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     7021 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc
     7081 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     7141 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     7201 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg gagctccacc
     7261 gcggtggcgg ccgctctaga attccattgc atacgttgta tccatatcat aatatgtaca
     7321 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
     7381 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     7441 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     7501 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     7561 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     7621 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     7681 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
     7741 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     7801 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     7861 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     7921 aggtctatat aagcagagct cgtttagtga accgcagtcg ctctgcggag aggctggcag
     7981 attgagccct gggaggttct ctccagcact agcaggtaga gcctgggtgt tccctgctag
     8041 actctcacca gcacttggcc agtgctgggc agagtggctc cacgcttgct tgcttaaaga
     8101 cctcttcaat aaagctgcca ttttagaagt aagccagtgt gtgttcccat ctctcctagt
     8161 cgccgcctgg tcaactcggt actcggtaat aagaagaccc tggtctgtta ggaccctttc
     8221 tgctttgaga aaccgaagca ggaaaatccc tagcagattg gcgcccgaac agggacttga
     8281 aggagagtga gagactcctg agtacggctg agtgaaggca gtaagggcgg caggaaccaa
     8341 ccacgacgga gtgctcctat aaaggcgcgg gtcggtacca gacggcgtga ggagcgggag
     8401 aggaggaggc ctccggttgc aggtaagtgc aacacaaaaa agaaatagct gtcttgttat
     8461 ccaggaaggg ataataagat agagtgggag atgggcgcga gaaactccgt cttgtcaggg
     8521 aagaaagcag atgaattgaa aaaattatta atcgcatgaa ttttaaaaga aggggaggaa
     8581 taggggatat gactccagca gaaagattaa ttaacatgat cactacagaa caagaaatac
     8641 aatttcaaca atcaaaaaac tcaaaattta aaaattttcg ggtctattac agagctcacg
     8701 cgtgattgga gttgggagat tataaattag tagagatcac tccgattggc ttggccccca
     8761 cagatgtgaa gaggtacact actggtggca cctcaagaaa taaaagaggg gtctttgtgc
     8821 tagggttctt gggttttctc gcaacggcag gttctgcaat gggcgcggcg tcgttgacgc
     8881 tgaccgctca gtcccggact ttattggctg ggatagtgca gcaacagcaa cagctgttgg
     8941 acgtggtcaa gagacaacaa gaattgttgc gactgaccgt ctggggaaca aagaacctcc
     9001 agactagggt cactgccatc gagaagtact taaaggacca ggcgcagcta aatgcttggg
     9061 gatgtgcgtt tagacaagtc tgccacacta ctgtaccatg gccaaatgca agtctaacac
     9121 cagactggaa caatgatact tggcaagagt gggagcgaaa ggttgacttc ttggaggaaa
     9181 atataacagc cctcctagaa gaggcacaaa ttcaacaaga gaagaacatg tatgaattac
     9241 aaaagttgaa tagctgggat gtgtttggca attggtttga ccttgcttct tggataaagt
     9301 atatacaata tggaatttat gtagttgtag gagtaatact gttaagaata gtgatctata
     9361 tagtac
//
