LOCUS       sequence-360575-        9108 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing HIV Envelope UFO.664.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9108)
  TITLE     Lentiviral vector expressing HIV envelope UFO.664
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGAE-UFO.664
FEATURES             Location/Qualifiers
     source          1..9108
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        21..324
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        325..528
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      668..677
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2671..3259
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2724..2744)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3142..3153)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        complement(4006..4024)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4045..4061)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4045..4061)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4058..4080)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4069..4085
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4093..4099,4100..4117,4118..4123))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4138..4159
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4276..4293)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4447..5035)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4527..4546)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5206..5997,5998..6066))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5829..5848
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6067..6171)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6197..6652)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6334..6355)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6546..6565
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6779..6801
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6793..6810
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6794..6810
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6817..6836
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6817..6835
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        6950..7329
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7330..7533
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 cgataagctt gggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
       61 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      121 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      181 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      241 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      301 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
      361 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
      421 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
      481 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
      541 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac
      601 cgatccagcc tccgcggccg ggaacggtgc attggaacgc ggattccccg tgccaagagt
      661 gagatccgcc accatggaca gagccaaact cctccttctc cttttgctcc tgctgctgcc
      721 tcaggcccag gccgtggaaa acctgtgggt caccgtgtac tacggcgtgc ccgtgtggaa
      781 ggacgccgag acaaccctgt tctgtgccag cgacgccaag gcctacgaca ccgaagtgcg
      841 gaatgtgtgg gccacccacg cctgcgtgcc caccgatcct aacccccagg aaatcgtgct
      901 ggaaaacgtg accgagaact tcaacatgtg gaagaacaac atggtggaac agatgcacac
      961 cgacatcatc agcctgtggg accagagcct gaagccctgc gtgaagctga cccctctgtg
     1021 cgtgaccctg aactgcacca acgtgaacgt gaccaacacc accaacaaca ccgaggaaaa
     1081 gggcgagatc aagaactgca gcttcaacat caccaccgag ctgcgggaca agaaaaagaa
     1141 ggtgtacgcc ctgttctacc ggctggacgt ggtgcccatc gacgataaca acaacaactc
     1201 cagcaattac cggctgatca actgcaacac cagcgccatc acccaggcct gccccaaggt
     1261 gtccttcgag cccatcccca tccactactg tgcccctgcc ggcttcgcca tcctgaagtg
     1321 caacgacaag aagttcaacg gcaccggccc ctgcaagaac gtgtccaccg tgcagtgtac
     1381 ccacggcatc aagcccgtgg tgtccaccca gctgctgctg aatggcagcc tggccgagga
     1441 agagatcatc atcagaagcg agaacatcac aaacaacgcc aagaccatca tcgtgcagct
     1501 gaacgagagc gtggaaatca attgcacccg gcccaacaac aataccagaa agagcatccg
     1561 gatcggccct ggccagtggt tttacgccac cggcgatatc atcggcgaca tcagacaggc
     1621 ccactgcaac atctccggca ccaagtggaa caagaccctg caacaggtcg tgaagaagct
     1681 gagagagcac ttcaacaaca agacaatcat cttcaacccc agctctggcg gcgacctgga
     1741 aatcaccacc cacagcttca attgtggcgg cgagttcttc tactgcaata cctccggcct
     1801 gttcaacagc acctggatcg gcaatggcac caagaacaac aacaacacca acgacaccat
     1861 caccctgccc tgcagaatca agcagatcat caatatgtgg cagcgcgtgg gccagcctat
     1921 gtacgcccct cctattcagg gcaagatcag atgcgtgtcc aatatcaccg gcctgctgct
     1981 gaccagggac ggcggaaaca acaacacaaa tgagacagag acattcagac ccggcggagg
     2041 cgacatgaga gacaactggc ggagcgagct gtacaagtac aaggtcgtga aaatcgagcc
     2101 cctgggcgtg gcccccaccc ggtgcaagag aagagtggtg gaaggcggcg gaggatctgg
     2161 cggaggggga tctgctgtgg gaatcggcgc tgtgttcctg ggctttctgg gagccgccgg
     2221 aagcacaatg ggcgctgcca gcatgaccct gaccgtgcag gccagaaatc tgctgtctgg
     2281 cggcagcggc tctggctctg gatctacagt gtgggggatc aagcagctgc aggctagagt
     2341 gctggctgtg gaaagatacc tgcgggacca gcagctgctg ggcatctggg gctgtagcgg
     2401 caagctgatc tgctgcacca atgtgccctg gaacagctcc tggtccaaca agagccagga
     2461 cgagatctgg gacaacatga cctggatgga atgggacaaa gagattaaca actacacaga
     2521 tatcatctac agcctgatcg aggaaagcca gaaccagcag gaaaagaacg agcaggacct
     2581 gctggccctg gactgatgag cggccgctcg acggtaccga gctcggatcc actagtaacg
     2641 gccgccagtg tgctggaatt cggctttgat aatcaacctc tggattacaa aatttgtgaa
     2701 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     2761 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     2821 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     2881 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     2941 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     3001 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     3061 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     3121 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     3181 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     3241 ctttgggccg cctccccgca tcggagccga attctgcaga tatccatcac actggcggcc
     3301 gctcgagttt tataaaagaa aaggggggac tggaagggat ttattacagt gcaagaagac
     3361 atagaatctt agacatgtac ttagaaaagg aagaaggcat cataccagat tggcaggatt
     3421 acacctcagg accaggaatt agatacccaa agacatttgg ctggctatgg aaattagtcc
     3481 ctgtaaatgt atcagatgag gcacaggagg atgagaggca ttatttaatg cagccagctc
     3541 aaacttccaa gtgggatgac ccttggggag aggttctagc gtggaagttt gatccaactc
     3601 tagcctacac ttatgaggca tatgctagat acccagaaga gttggaagca agtcaggcct
     3661 gtcagaactg catttcgctc tgtattcagt cgctctgcgg agaggctggc agattgagcc
     3721 ctgggaggtt ctctccagca ctagcaggta gagcctgggt gttccctgct agactctcac
     3781 cagcacttgg ccagtgctgg gcagagtggc tccacgcttg cttgcttaaa gacctcttca
     3841 ataaagctgc cattttagaa gtaagccagt gtgtgttccc atctctccta gtcgccgcct
     3901 ggtcaactcg gtactcggta ataagaagac cctggtctgt taggaccctt tctgctttga
     3961 gaaaccgaag caggaaaatc cctagcatgg taccagcttt tgttcccttt agtgagggtt
     4021 aatttcgagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     4081 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     4141 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     4201 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     4261 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     4321 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     4381 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     4441 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     4501 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     4561 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     4621 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     4681 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     4741 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     4801 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     4861 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     4921 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     4981 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     5041 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     5101 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     5161 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     5221 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     5281 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     5341 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     5401 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     5461 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     5521 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     5581 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     5641 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     5701 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     5761 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     5821 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     5881 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     5941 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     6001 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     6061 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     6121 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     6181 ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     6241 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     6301 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     6361 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     6421 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     6481 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     6541 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     6601 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg
     6661 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     6721 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     6781 cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa
     6841 ttggagctcc accgcggtgg cggccgctct agaattccat tgcatacgtt gtatccatat
     6901 cataatatgt acatttatat tggctcatgt ccaacattac cgccatgttg acattgatta
     6961 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     7021 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     7081 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     7141 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     7201 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     7261 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     7321 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     7381 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     7441 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     7501 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgcag tcgctctgcg
     7561 gagaggctgg cagattgagc cctgggaggt tctctccagc actagcaggt agagcctggg
     7621 tgttccctgc tagactctca ccagcacttg gccagtgctg ggcagagtgg ctccacgctt
     7681 gcttgcttaa agacctcttc aataaagctg ccattttaga agtaagccag tgtgtgttcc
     7741 catctctcct agtcgccgcc tggtcaactc ggtactcggt aataagaaga ccctggtctg
     7801 ttaggaccct ttctgctttg agaaaccgaa gcaggaaaat ccctagcaga ttggcgcccg
     7861 aacagggact tgaaggagag tgagagactc ctgagtacgg ctgagtgaag gcagtaaggg
     7921 cggcaggaac caaccacgac ggagtgctcc tataaaggcg cgggtcggta ccagacggcg
     7981 tgaggagcgg gagaggagga ggcctccggt tgcaggtaag tgcaacacaa aaaagaaata
     8041 gctgtcttgt tatccaggaa gggataataa gatagagtgg gagatgggcg cgagaaactc
     8101 cgtcttgtca gggaagaaag cagatgaatt gaaaaaatta ttaatcgcat gaattttaaa
     8161 agaaggggag gaatagggga tatgactcca gcagaaagat taattaacat gatcactaca
     8221 gaacaagaaa tacaatttca acaatcaaaa aactcaaaat ttaaaaattt tcgggtctat
     8281 tacagagctc acgcgtgatt ggagttggga gattataaat tagtagagat cactccgatt
     8341 ggcttggccc ccacagatgt gaagaggtac actactggtg gcacctcaag aaataaaaga
     8401 ggggtctttg tgctagggtt cttgggtttt ctcgcaacgg caggttctgc aatgggcgcg
     8461 gcgtcgttga cgctgaccgc tcagtcccgg actttattgg ctgggatagt gcagcaacag
     8521 caacagctgt tggacgtggt caagagacaa caagaattgt tgcgactgac cgtctgggga
     8581 acaaagaacc tccagactag ggtcactgcc atcgagaagt acttaaagga ccaggcgcag
     8641 ctaaatgctt ggggatgtgc gtttagacaa gtctgccaca ctactgtacc atggccaaat
     8701 gcaagtctaa caccagactg gaacaatgat acttggcaag agtgggagcg aaaggttgac
     8761 ttcttggagg aaaatataac agccctccta gaagaggcac aaattcaaca agagaagaac
     8821 atgtatgaat tacaaaagtt gaatagctgg gatgtgtttg gcaattggtt tgaccttgct
     8881 tcttggataa agtatataca atatggaatt tatgtagttg taggagtaat actgttaaga
     8941 atagtgatct atatagtaca aatgctagct aagttaaggc aggggtatag gccagtgttc
     9001 tcttccccac cctcttattt ccagtagact catacccaac aggacccggc actgccaacc
     9061 agagaaggca aagaaggaga cggtggagaa ggcggtggat cctattat
//
