LOCUS       sequence-360711-       10317 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sp.Cas9 and gRNA yeast expression vector withXII-5 gRNA pre-cloned. 
            Selection =SplitKanamycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10317)
  AUTHORS   Bean BDM, Whiteway M, Martin VJJ
  TITLE     The MyLo CRISPR-Cas9 Toolkit: A Markerless Yeast Localization and 
            Overexpression CRISPR-Cas9 Toolkit.
  JOURNAL   G3 (Bethesda). 2022 Jun 16. pii: 6609175. doi: 
            10.1093/g3journal/jkac154.
  PUBMED    35708612
REFERENCE   2  (bases 1 to 10317)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Jun 16. pii: 6609175. doi: 
            10.1093/g3journal/jkac154."
COMMENT     Sequence Label: pBBK14 pCAS-Tyr-[gRNA: 4=XII-5] (SplitKanR, AmpR)
FEATURES             Location/Qualifiers
     source          1..10317
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(962..1009)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_RNA        1382..1457
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        1591..1933
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     complement(2002..2021)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      3268..3465
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(join(3616..4407,4408..4476))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4239..4258
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4477..4581)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5284..9387
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5284..9387
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             9397..9417
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9433..9456
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     terminator      9460..9649
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(9706..10294)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9786..9805)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgaagcatct gtgcttcatt ttgtagaaca aaaatgcaac gcgagagcgc taatttttca
       61 aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgaaag cgctatttta
      121 ccaacgaaga atctgtgctt catttttgta aaacaaaaat gcaacgcgag agcgctaatt
      181 tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc gagagcgcta
      241 ttttaccaac aaagaatcta tacttctttt ttgttctaca aaaatgcatc ccgagagcgc
      301 tatttttcta acaaagcatc ttagattact ttttttctcc tttgtgcgct ctataatgca
      361 gtctcttgat aactttttgc actgtaggtc cgttaaggtt agaagaaggc tactttggtg
      421 tctattttct cttccataaa aaaagcctga ctccacttcc cgcgtttact gattactagc
      481 gaagctgcgg gtgcattttt tcaagataaa ggcatccccg attatattct ataccgatgt
      541 ggattgcgca tactttgtga acagaaagtg atagcgttga tgattcttca ttggtcagaa
      601 aattatgaac ggtttcttct attttgtctc tatatactac gtataggaaa tgtttacatt
      661 ttcgtattgt tttcgattca ctctatgaat agttcttact acaatttttt tgtctaaaga
      721 gtaatactag agataaacat aaaaaatgta gaggtcgagt ttagatgcaa gttcaaggag
      781 cgaaaggtgg atgggtaggt tatataggga tatagcacag agatatatag caaagagata
      841 cttttgagca atgtttgtgg aagcggtatt cgcaatattt tagtagcccg ttacagtccg
      901 gtgcgttttt ggttttttga aagtgcgtct tcagagcgct tttggttttc aaaagcgctc
      961 tgaagttcct atactttcta gagaatagga acttcggaat aggaacttca aagcgtttcc
     1021 gaaaacgagc gcttccgaaa atgcaacgcg agctgcgcac atacagctca ctgttcacgt
     1081 cgcacctata tctgcgtgtt gcctgtatat atatatacat gagaagaacg gcatagtgcg
     1141 tgtttatgct taaatgcgta tatgtgttat gtagtatact ctttcttcaa caattaaata
     1201 ctctcggtag ccaagttggt ttaaggcgca agactgtaat ttatcactac gaaatcttga
     1261 gatcgggcgt tcgactcgcc cccgggagag atggccggca tggtcccagc ctcctcgctg
     1321 gcgccggctg ggcaacacct tcgggtggcg aatgggactt tttgtcacag tgtcacatca
     1381 ggttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag
     1441 tggcaccgag tcggtgcttt ttttattttt tgtcactatt gttatgtaaa atgccacctc
     1501 tgacagtatg gaacgcaaac ttctgtctag tggatatagg tctagagatc tgtttagctt
     1561 gcctcgtccc cgccgggtca cccggccagc gacatggagg cccagaatac cctccttgac
     1621 agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtacat ttagcccata
     1681 catccccatg tataatcatt tgcatccata cattttgatg gccgcacggc gcgaagcaaa
     1741 aattacggct cctcgctgca gacctgcgag cagggaaacg ctcccctcac agacgcgttg
     1801 aattgtcccc acgccgcgcc cctgtagaga aatataaaag gttaggattt gccactgagg
     1861 ttcttctttc atatacttcc ttttaaaatc ttgctaggat acagttctca catcacatcc
     1921 gaacataaac aacaatgggt aaggaaaaga ctcacgtttc gaggccgcga ttaaattcca
     1981 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg
     2041 cgacaatcta tcgattgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca
     2101 aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat
     2161 ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca
     2221 ccactgcgat ccccggcaaa acagcattcc aggtattaga agaatatcct gattcaggtg
     2281 aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta
     2341 attgtccttt taacagcgat cgcgtatttc gtctggctca ggcgcaatca cgaatgaata
     2401 acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag
     2461 tctggaaaga aatgcataag cttttgccat tctcaccgga ttcagtcgtc actcatggtg
     2521 atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg
     2581 gacgagtcgg aatcgacgtc gctgcctgca ggatggcaaa ggtagcgttg ccaatgatgt
     2641 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
     2701 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggcaaaac
     2761 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
     2821 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg
     2881 cgtatttcgt ctggctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
     2941 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataagct
     3001 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
     3061 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
     3121 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
     3181 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
     3241 gatgctcgat gagtttttct aatcagtact gacaataaaa agattcttgt tttcaagaac
     3301 ttgtcatttg tatagttttt ttatattgta gttgttctat tttaatcaaa tgttagcgtg
     3361 atttatattt tttttcgcct cgacatcatc tgcccagatg cgaagttaag tgcgcagaaa
     3421 gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta tactgctgtc gattcgatac
     3481 taacgccgcc atccagtgtc gaaaacgagc tctcgagaac ccttaatcaa aaaggatctt
     3541 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     3601 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     3661 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     3721 cttaccatct ggccccagtg ctgcaatgat accgcgggac ccacgctcac cggctccaga
     3781 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     3841 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     3901 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     3961 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     4021 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     4081 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     4141 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     4201 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     4261 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     4321 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     4381 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     4441 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     4501 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     4561 taaacaaata ggggttcgtc gacagtcgaa caagaagcag gcaaagttta gagcactgcc
     4621 cctccgcact caaaaaagaa aaaactagga ggaaaataaa attctcaacc acacaaacac
     4681 ataaacacat acaaatacaa atacaagctt atttacttga catcgcgcga tcttccacta
     4741 ttcagcgccg tccgccctct ctcgtgtttt ttgtttacgc gacaactatg cgaaatccgg
     4801 agcaacgggc aaccgtttgg ggaaagacca cacccacgcg cgatcgccat ggcaacgagg
     4861 tcgcacacgc cccacaccca gacctccctg cgagcgggca tgggtacaat gtccccgttg
     4921 ccacagacac cacttcgtag cacagcgcag agcgtagcgt gttgttgctg ctgacaaaag
     4981 aaaatttttc ttagcaaagc aaaggagggg aagcacgggc agatagcacc gtaccatacc
     5041 cttggaaact cgaaatgaac gaagcaggaa atgagagaat gagagttttg taggtatata
     5101 tagcggtagt gtttgcgcgt taccatcatc ttctggatct atctattgtt cttttcctca
     5161 tcactttccc ctttttcgct cttcttcttg tcttttattt ctttcttttt tttaattgtt
     5221 ccctcgattg gctatctacc aaagaatcca aacttaatac acgtatttat ttgtccaatt
     5281 accatggata agaaatactc aataggctta gatatcggca caaatagcgt cggatgggcg
     5341 gtgatcactg atgaatataa ggttccgtct aaaaagttca aggttctggg aaatacagac
     5401 cgccacagta tcaaaaaaaa tcttataggg gctcttttat ttgacagtgg agagacagcg
     5461 gaagcgactc gactcaaacg gacagctcgt agaaggtata cacgtcggaa gaatcgtatt
     5521 tgttatctac aggagatttt ttcaaatgag atggcgaaag tagatgatag tttctttcat
     5581 cgacttgaag agtctttttt ggtggaagaa gacaagaagc atgaacgtca tcctattttt
     5641 ggaaatatag tagatgaagt tgcttatcat gagaaatatc caactatcta tcatctgcga
     5701 aaaaaattgg tagattctac tgataaagcg gatttgcgct taatctattt ggccttagcg
     5761 catatgatta agtttcgtgg tcattttttg attgagggag atttaaatcc tgataatagt
     5821 gatgtggaca aactatttat ccagttggta caaacctaca atcaattatt tgaagaaaac
     5881 cctattaacg caagtggagt agatgctaaa gcgattcttt ctgcacgatt gagtaaatca
     5941 agacgattag aaaatctcat tgctcagctc cccggtgaga agaaaaatgg cttatttggg
     6001 aatctcattg ctttgtcatt gggtttgacc cctaatttta aatcaaattt tgatttggca
     6061 gaagatgcta aattacagct ttcaaaagat acttacgatg atgatttaga taatttattg
     6121 gcgcaaattg gagatcaata tgctgatttg tttttggcag ctaagaattt atcagatgct
     6181 attttacttt cagatatcct aagagtaaat actgaaataa ctaaggctcc cctatcagct
     6241 tcaatgatta aacgctacga tgaacatcat caagacttga ctcttttaaa agctttagtt
     6301 cgacaacaac ttccagaaaa gtataaagaa atcttttttg atcaatcaaa aaacggatat
     6361 gcaggttata ttgatggggg agctagccaa gaagaatttt ataaatttat caaaccaatt
     6421 ttagaaaaaa tggatggtac tgaggaatta ttggtgaaac taaatcgtga agatttgctg
     6481 cgcaagcaac ggacctttga caacggctct attccccatc aaattcactt gggtgagctg
     6541 catgctattt tgagaagaca agaagacttt tatccatttt taaaagacaa tcgtgagaag
     6601 attgaaaaaa tcttgacttt tcgaattcct tattatgttg gtccattggc gcgtggcaat
     6661 agtcgttttg catggatgac tcggaagtct gaagaaacaa ttaccccatg gaattttgaa
     6721 gaagttgtcg ataaaggtgc ttcagctcaa tcatttattg aacgcatgac aaactttgat
     6781 aaaaatcttc caaatgaaaa agtactacca aaacatagtt tgctttatga gtattttacg
     6841 gtttataacg aattgacaaa ggtcaaatat gttactgaag gaatgcgaaa accagcattt
     6901 ctttcaggtg aacagaagaa agccattgtt gatttactct tcaaaacaaa tcgaaaagta
     6961 accgttaagc aattaaaaga agattatttc aaaaaaatag aatgttttga tagtgttgaa
     7021 atttcaggag ttgaagatag atttaatgct tcattaggta cctaccatga tttgctaaaa
     7081 attattaaag ataaagattt tttggataat gaagaaaatg aagatatctt agaggatatt
     7141 gttttaacat tgaccttatt tgaagatagg gagatgattg aggaaagact taaaacatat
     7201 gctcacctct ttgatgataa ggtgatgaaa cagcttaaac gtcgccgtta tactggttgg
     7261 ggacgtttgt ctcgaaaatt gattaatggt attagggata agcaatctgg caaaacaata
     7321 ttagattttt tgaaatcaga tggttttgcc aatcgcaatt ttatgcagct gatccatgat
     7381 gatagtttga catttaaaga agacattcaa aaagcacaag tgtctggaca aggcgatagt
     7441 ttacatgaac atattgcaaa tttagctggt agccctgcta ttaaaaaagg tattttacag
     7501 actgtaaaag ttgttgatga attggtcaaa gtaatggggc ggcataagcc agaaaatatc
     7561 gttattgaaa tggcacgtga aaatcagaca actcaaaagg gccagaaaaa ttcgcgagag
     7621 cgtatgaaac gaatcgaaga aggtatcaaa gaattaggaa gtcagattct taaagagcat
     7681 cctgttgaaa atactcaatt gcaaaatgaa aagctctatc tctattatct ccaaaatgga
     7741 agagacatgt atgtggacca agaattagat attaatcgtt taagtgatta tgatgtcgat
     7801 cacattgttc cacaaagttt ccttaaagac gattcaatag acaataaggt cttaacgcgt
     7861 tctgataaaa atcgtggtaa atcggataac gttccaagtg aagaagtagt caaaaagatg
     7921 aaaaactatt ggagacaact tctaaacgcc aagttaatca ctcaacgtaa gtttgataat
     7981 ttaacgaaag ctgaacgtgg aggtttgagt gaacttgata aagctggttt tatcaaacgc
     8041 caattggttg aaactcgcca aatcactaag catgtggcac aaattttgga tagtcgcatg
     8101 aatactaaat acgatgaaaa tgataaactt attcgagagg ttaaagtgat taccttaaaa
     8161 tctaaattag tttctgactt ccgaaaagat ttccaattct ataaagtacg tgagattaac
     8221 aattaccatc atgcccatga tgcgtatcta aatgccgtcg ttggaactgc tttgattaag
     8281 aaatatccaa aacttgaatc ggagtttgtc tatggtgatt ataaagttta tgatgttcgt
     8341 aaaatgattg ctaagtctga gcaagaaata ggcaaagcaa ccgcaaaata tttcttttac
     8401 tctaatatca tgaacttctt caaaacagaa attacacttg caaatggaga gattcgcaaa
     8461 cgccctctaa tcgaaactaa tggggaaact ggagaaattg tctgggataa agggcgagat
     8521 tttgccacag tgcgcaaagt attgtccatg ccccaagtca atattgtcaa gaaaacagaa
     8581 gtacagacag gcggattctc caaggagtca attttaccaa aaagaaattc ggacaagctt
     8641 attgctcgta aaaaagactg ggatccaaaa aaatatggtg gttttgatag tccaacggta
     8701 gcttattcag tcctagtggt tgctaaggtg gaaaaaggga aatcgaagaa gttaaaatcc
     8761 gttaaagagt tactagggat cacaattatg gaaagaagtt cctttgaaaa aaatccgatt
     8821 gactttttag aagctaaagg atataaggaa gttaaaaaag acttaatcat taaactacct
     8881 aaatatagtc tttttgagtt agaaaacggt cgtaaacgga tgctggctag tgccggagaa
     8941 ttacaaaaag gaaatgagct ggctctgcca agcaaatatg tgaatttttt atatttagct
     9001 agtcattatg aaaagttgaa gggtagtcca gaagataacg aacaaaaaca attgtttgtg
     9061 gagcagcata agcattattt agatgagatt attgagcaaa tcagtgaatt ttctaagcgt
     9121 gttattttag cagatgccaa tttagataaa gttcttagtg catataacaa acatagagac
     9181 aaaccaatac gtgaacaagc agaaaatatt attcatttat ttacgttgac gaatcttgga
     9241 gctcccgctg cttttaaata ttttgataca acaattgatc gtaaacgata tacgtctaca
     9301 aaagaagttt tagatgccac tcttatccat caatccatca ctggtcttta tgaaacacgc
     9361 attgatttga gtcagctagg aggtgacggt ggagggccaa aaaagaaaag aaaagttgaa
     9421 gatgcttctg ctcatcatca ccatcaccat caccattaaa tccgctctaa ccgaaaagga
     9481 aggagttaga caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt
     9541 aagaacgtta tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg
     9601 taacattata ctgaaaacct tgcttgagaa ggttttggga cgctcgaagt gagcaaaagg
     9661 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     9721 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     9781 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     9841 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     9901 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     9961 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
    10021 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
    10081 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    10141 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    10201 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    10261 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctac
//
