LOCUS       sequence-360901-        6365 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector containing a yeast integration cassette for RPL18B-driven 
            expression of SNF7-Link-Scarlet(rfp), a marker for the endosome, 
            class E compartment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6365)
  AUTHORS   Bean BDM, Whiteway M, Martin VJJ
  TITLE     The MyLo CRISPR-Cas9 Toolkit: A Markerless Yeast Localization and 
            Overexpression CRISPR-Cas9 Toolkit.
  JOURNAL   G3 (Bethesda). 2022 Jun 16. pii: 6609175. doi: 
            10.1093/g3journal/jkac154.
  PUBMED    35708612
REFERENCE   2  (bases 1 to 6365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Jun 16. pii: 6609175. doi: 
            10.1093/g3journal/jkac154."
COMMENT     Sequence Label: pBBK78
            pHomL-RPL18Bp-SNF7-Link-Scarlet(rfp)-SS...
FEATURES             Location/Qualifiers
     source          1..6365
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(1..792,793..861))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     624..643
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(862..966)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1069..1657
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1558..1577
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
       61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      121 cagtgctgca atgataccgc gggacccacg ctcaccggct ccagatttat cagcaataaa
      181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
      661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      961 tccgcgcaca tttccccgaa aagtgccacc tgtcatgacc aaaatccctt aacgtgagtt
     1021 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1081 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     1141 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     1201 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     1261 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     1321 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     1381 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     1441 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     1501 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     1561 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     1621 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     1681 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     1741 ttctgtggat aaccgtgcgg ccgccaatgt tatctattgg cagatgttca tacggggctt
     1801 tcaaatattg atgaaattat gtgatgttta gaagaagatt cgaactgttt tcagtagatt
     1861 tggtaactgt gcaaccataa ctcatgcccc ctatttatcg gcattgagat tgtaaaggaa
     1921 gtaaaacgca tttttgcaga tctgttctct tatgtatttt taatcgtcct tgtatggaag
     1981 tatcaaaggg gacgttcttc acctccttgg aaaacgcaaa ctttcttttt gcctatcaca
     2041 gtatcttatc gagctaacta ttttcgacac acatgaaaaa gcagaaatat taacgaaaaa
     2101 gaaaagaaag accatgtcat gtacgggcaa tcagaatatg caagatgagc gggaacgggc
     2161 gaaatgtgca cgcgcacaac ttacgaaacg cggatgagtc actgacagcc accgcagagg
     2221 ttctgactcc tactgagctc tattggaggt ggcagaaccg gtaatcccac agtgagcaac
     2281 ttgacaaacc aacagatata ggctgtgtct taaaatatac catccaatac cttgatgaac
     2341 ttttcaaaga tgactaaaat aagtgaaatt tcaacattaa cttcgagctg taaacacagt
     2401 ggtttctttg cataaacacc atcagcctca agtcgtcaag taaagatttc gtgttcatgc
     2461 agatagataa caatctatat gttgataatt agcgttgcct catcaatgcg tacacgaaga
     2521 atatactaaa aaatgagcag gcaagataaa cgaaggcaaa gatgacagag cagaaagccc
     2581 tagtaaagcg tattacaaat gaaaccaaga ttcagattgc gatccctgaa ttcgcatcta
     2641 gatggtagag ccacaaacag ccggtacaag caacgatctc caggaccatc tgaatcatgc
     2701 gcggatgaca cgaactcacg acggcgatca cagacattaa cccacagtac agacactgcg
     2761 acaacgtggc aattcgtcgc aataccgtct cactgaactg gccgataatt gcagacgaac
     2821 gaagaggatg tccaatattt tttttaagga ataaggatac ttcaagacta gattcccccc
     2881 tgcattccca tcagaaccgt aaaccttggc gctttccttg ggaagtattc aagaagtgcc
     2941 ttgtccggtt tctgtggctc acaaaccagc gcgcccgata tggctttctt ttcacttatg
     3001 aatgtaccag tacgggacaa ttagaacgct cctgtaacaa tctctttgca aatgtggggt
     3061 tacattctaa ccatgtcaca ctgctgacga aattcaaagt aaaaaaaaat gggaccacgt
     3121 cttgagaacg atagattttc tttattttac attgaacagt cgttgtctca gcgcgcttta
     3181 tgttttcatt catacttcat attataaaat aacaaaagaa gaatttcata ttcacgccca
     3241 agaaatcagg ctgctttcca aatgcaattg acacttcatt agccatcaca caaaactctt
     3301 tcttgctgga gcttctttta aaaaagacct cagtacacca aacacgttac ccgacctcgt
     3361 tattttacga caactatgat aaaattctga agaaaaaata aaaaaatttt catacttctt
     3421 gcttttattt aaaccattga atgatttctt ttgaacaaaa ctacctgttt caccaaagga
     3481 aatagaaaga aaaaatcaat tagaagaaaa caaaaaacaa aagatctatg tggtcatcac
     3541 tttttggttg gacatcaagt aatgccaaga ataaagagtc accaacaaag gccatagtgc
     3601 ggttgaggga gcatatcaac cttctatcca aaaagcaatc gcatttacgt actcaaatta
     3661 caaatcaaga gaatgaagct agaatctttt tgacgaaggg caataaagta atggcgaaga
     3721 atgcacttaa aaagaagaag acaatcgaac aacttttaag taaggtagaa ggcacaatgg
     3781 agtctatgga acagcagcta ttctctatag aaagtgcaaa cttaaatcta gagacaatga
     3841 gggctatgca ggaaggtgct aaggcaatga aaactattca cagtggcctt gacatagata
     3901 aagtggatga aactatggac gagataaggg agcaagtcga attaggagat gaaataagcg
     3961 acgctatatc caggccttta attactgggg caaacgaggt ggatgaagat gagctggacg
     4021 aggaattgga catgctggct caagaaaatg ctaaccaaga aacgtccaag atcgttaata
     4081 ataatgttaa tgcggcgcct atctcagaga acaaagtctc actacctagt gttccaagta
     4141 ataaaattaa acaaagtgag aactctgtga aggacgggga agaggaagag gatgaagaag
     4201 atgaagatga aaaagcatta agagaactac aagcagaaat ggggcttgga tccggtcctt
     4261 ctggcccaac tggcatggtt agtaaaggtg aagctgttat aaaagaattc atgaggttta
     4321 aagttcatat ggaaggttca atgaatggtc atgaatttga aattgaaggt gaaggtgaag
     4381 gtagaccata tgaaggtaca caaactgcta aattgaaagt tactaaaggt ggtccattac
     4441 cattttcttg ggatattttg tctccacaat tcatgtatgg ttctagagct tttattaagc
     4501 atccagctga tattccagat tattataaac aatcttttcc tgaaggtttt aaatgggaaa
     4561 gagttatgaa tttcgaagat ggtggtgctg ttactgttac tcaagatact tctttggaag
     4621 atggtacttt aatctataaa gttaaattga gaggtactaa ttttccacca gatggtccag
     4681 ttatgcaaaa gaaaactatg ggttgggaag catctactga aagattgtat ccagaagatg
     4741 gtgttttgaa aggagacatt aagatggctt tgagattgaa agatggtggt cggtacctgg
     4801 ccgacttcaa gaccacctat aaagctaaaa aaccagttca aatgccaggt gcatataatg
     4861 ttgatagaaa gttagatata acgtcgcata acgaggacta tactgtagtt gaacaatatg
     4921 aacgtagtga aggtagacat agtaccggag gaatggatga attgtataaa tagctcgagt
     4981 ggcgccaatt ggtgcggcaa ttgataataa cgaaaatgtc ttttaatgat ctgggtataa
     5041 tgaggaattt tccgaacgtt tttactttat atatatatat acatgtaaca tatattctat
     5101 acgctataga gaaaggaaat ttttcaatta aaaaaaaaat agagaaagag tttcacttct
     5161 tgattatcgc taacactaat ggttgaagta ctgctacttt aattttatgc tgccaatgag
     5221 acgacggggt catcacggct catcatgcgc caaacaaatg tgtgcaatac acgctcggat
     5281 gactgcatga tgaccgcact gactggggac agcagatcca cctaagcctg tgagagaagc
     5341 agacacccga cagatcaagg cagttaacta gtgcactgca gtacaaatcg tccccaacaa
     5401 aagtgggctc tcaaaattca tcacatttaa atgcatatag gaagagcaac agttggtttg
     5461 catctgatgt tccttaaaga tttcgacata atgtgcgaag tagatccctt cagcactgta
     5521 cttgtttttg cgactagatt gtaaatcatt ctttatttaa tctctttctt taactactgc
     5581 ttaaagtata atttggtccg tagtttaata actatactaa gcgaacgctc tctcgaaatt
     5641 tttcttaacg cgtccttgta ctgcgtctaa cgcttttgcc acttggattt ctattatagg
     5701 aaatagtctc acttactggg cgacgaattt tcgcgttttg atgaagcaca ggaagaattt
     5761 ctttatgctc agaagtttga cagcaagcaa gttcatcatt cgaactagcc ttattgtttt
     5821 agttcagtga cagcgaactg ccgtactcga tgctttattt ctcacggtag agcggaagaa
     5881 cagatagatc cctacatatt gctaccactt ctattacaca atagtttcaa tagcttgcag
     5941 cgtagctaaa ctctaaaatt tatctaaatc actcatataa accgaaccct tccccttccg
     6001 cttatagtac agctgatgct ccttcactat tttaacatgt ggaattcttg aaagaatgaa
     6061 atcgccatgc caagccatca cacggtcttt tatgcaattg attgaccgcc tgcaacacat
     6121 aggcagtaaa atttttacaa gcagtagcag aacaggccac acaatcgcaa gtgattaacg
     6181 tccacacagg tatagggttt ctggaccata tgatacatgc tctggccaag cattccggct
     6241 ggtcgctaat cgttgagtgc cgagcggccg cgattatcaa aaaggatctt cacctagatc
     6301 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     6361 gacag
//
